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▼Recombinant Antibodies
Also available for purchase in our catalog, we have flexible recombinant antibodies that are excellent tools in many different types of experiments, and well-suited for those that are large-scale or long-term. Our collection of recombinant antibodies includes different forms/formats of said antibodies, such as labeled or purified versions, and can be made utilizing different species’ isotypes to suit various experimental requirements.
Viewing 250-300 of 3337 product results
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PPP2R5E staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
PPP2R5E, Monoclonal Recombinant Antibody (Cat# AAA266430)
Full Name
Recombinant Anti-PPP2R5E Rabbit mAb
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemisry)
(Immunochemical staining of human CD4 in human lymph node with rabbit monoclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
CD4, Monoclonal Recombinant Antibody (Cat# AAA259408)
Full Name
Recombinant Anti-CD4 Antibody, Rabbit Monoclonal
Gene Names
CD4; CD4mut
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A
Pricing
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with hydroxyurea (4mm, 24h, +)(line B); treated with hydroxyurea and lambda-phosphatase (line C) using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution.)
cdc2, Monoclonal Recombinant Antibody (Cat# AAA259508)
Full Name
Phospho-cdc2 (Tyr15) Antibody, Rabbit MAb
Reactivity
Human
Applications
Western Blot
Purity
Protein A
Pricing
FCM/FACS (Flow Cytometry)
(Untransfected CT26 cells (green line) and transfected Human ROR1 CT26 stable cells (red line) were stained with anti-ROR1 antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
ROR1, Monoclonal Recombinant Antibody (Cat# AAA279017)
Full Name
ROR1 Recombinant Monoclonal Antibody
Gene Names
ROR1; NTRKR1; dJ537F10.1
Reactivity
Human
Applications
ELISA
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279126 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ACTA1, Monoclonal Recombinant Antibody (Cat# AAA279126)
Full Name
ACTA1 Recombinant Monoclonal Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279134 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WDR5, Monoclonal Recombinant Antibody (Cat# AAA279134)
Full Name
WDR5 Recombinant Monoclonal Antibody
Gene Names
WDR5; SWD3; BIG-3; CFAP89
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF-7 cells stained with AAA279055 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WFDC2, Monoclonal Recombinant Antibody (Cat# AAA279055)
Full Name
WFDC2 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279156 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SNRPD1, Monoclonal Recombinant Antibody (Cat# AAA279156)
Full Name
SNRPD1 Recombinant Monoclonal Antibody
Gene Names
SNRPD1; SMD1; SNRPD; Sm-D1; HsT2456
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of NIH/3T3 cell with AAA279163 at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
VIM, Monoclonal Recombinant Antibody (Cat# AAA279163)
Full Name
VIM Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279164 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,001 events was performed.)
HIF1A, Monoclonal Recombinant Antibody (Cat# AAA279164)
Full Name
HIF1A Recombinant Monoclonal Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
IHC (Immunohistochemisry)
(IHC image of AAA279317 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.)
HGF, Monoclonal Recombinant Antibody (Cat# AAA279317)
Full Name
HGF Recombinant Monoclonal Antibody
Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-chromatography
Pricing
Bioactivity
(The Binding Activity of Human CD79A&CD79B with Anti-CD79B recombinant antibodyActivity:Measured by its binding ability in a functional ELISA. Immobilized Human CD79A&CD79B at 2ug/mL can bind Anti-CD79B recombinant antibody. The EC50 is 14.85-17.47ng/mL.)
CD79B, Monoclonal Recombinant Antibody (Cat# AAA279340)
Full Name
CD79B Recombinant Monoclonal Antibody
Gene Names
CD79B; B29; IGB; AGM6
Reactivity
Human
Applications
ELISA
Purity
Affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MST4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
MST4, Monoclonal Recombinant Antibody (Cat# AAA266422)
Full Name
Recombinant Anti-MST4 Rabbit mAb
Gene Names
MST4; MASK
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SMAD5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
SMAD5, Monoclonal Recombinant Antibody (Cat# AAA266342)
Full Name
Recombinant Anti-SMAD5 Rabbit mAb
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of ENPP5 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
ENPP5, Monoclonal Recombinant Antibody (Cat# AAA266354)
Full Name
Recombinant Anti-ENPP5 Rabbit mAb
Gene Names
ENPP5; KIAA0879
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of RhoA/B/C staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
RhoA/B/C, Monoclonal Recombinant Antibody (Cat# AAA266355)
Full Name
Recombinant Anti-RhoA/B/C Rabbit mAb
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MAD2L2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
MAD2L2, Monoclonal Recombinant Antibody (Cat# AAA266379)
Full Name
Recombinant Anti-MAD2L2 Rabbit mAb
Gene Names
MAD2L2; REV7; MAD2B; POLZ2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of STAU1 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
STAU1, Monoclonal Recombinant Antibody (Cat# AAA266300)
Full Name
Recombinant Anti-STAU1 Rabbit mAb
Gene Names
STAU1; STAU; PPP1R150
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279155 diluted at 1:50 and staining in paraffin-embedded human varian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
TK1, Monoclonal Recombinant Antibody (Cat# AAA279155)
Full Name
TK1 Recombinant Monoclonal Antibody
Gene Names
TK1; TK2
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279091 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
STAT1, Monoclonal Recombinant Antibody (Cat# AAA279091)
Full Name
STAT1 Recombinant Monoclonal Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279112 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
KRT6A, Monoclonal Recombinant Antibody (Cat# AAA279112)
Full Name
KRT6A Recombinant Monoclonal Antibody
Gene Names
KRT6A; K6A; K6C; K6D; PC3; CK6A; CK6C; CK6D; KRT6C; KRT6D
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279138 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
TP53, Monoclonal Recombinant Antibody (Cat# AAA279138)
Full Name
TP53 Recombinant Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279139 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GAPDH, Monoclonal Recombinant Antibody (Cat# AAA279139)
Full Name
GAPDH Recombinant Monoclonal Antibody
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC3 cells stained with AAA279322 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
MIF, Monoclonal Recombinant Antibody (Cat# AAA279322)
Full Name
MIF Recombinant Monoclonal Antibody
Gene Names
MIF; GIF; GLIF; MMIF
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity-chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC-3 cells stained with AAA279341 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FGF1, Monoclonal Recombinant Antibody (Cat# AAA279341)
Full Name
FGF1 Recombinant Monoclonal Antibody
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279344 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ICAM1, Monoclonal Recombinant Antibody (Cat# AAA279344)
Full Name
ICAM1 Recombinant Monoclonal Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Affinity chromatography
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of STAG2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
STAG2, Monoclonal Recombinant Antibody (Cat# AAA266362)
Full Name
Recombinant Anti-STAG2 Rabbit mAb
Gene Names
STAG2; SA2; SA-2; SCC3B; bA517O1.1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PAX6 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
PAX6, Monoclonal Recombinant Antibody (Cat# AAA266365)
Full Name
Recombinant Anti-PAX6 Rabbit mAb
Gene Names
PAX6; AN; AN2; MGDA; WAGR; D11S812E
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BRD4 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
BRD4, Monoclonal Recombinant Antibody (Cat# AAA266371)
Full Name
Recombinant Anti-BRD4 Rabbit mAb
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of p38 staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
p38, Monoclonal Recombinant Antibody (Cat# AAA266280)
Full Name
Recombinant Anti-p38 Rabbit mAb
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD71 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
CD71, Monoclonal Recombinant Antibody (Cat# AAA266393)
Full Name
Recombinant Anti-CD71 Rabbit mAb
Gene Names
TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Cathepsin H/K/L/V staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
Cathepsin H/K/L/V, Monoclonal Recombinant Antibody (Cat# AAA266396)
Full Name
Recombinant Anti-Cathepsin H/K/L/V Rabbit mAb
Gene Names
CTSH; CPSB; ACC-4; ACC-5; minichain
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of TRIM25 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
TRIM25, Monoclonal Recombinant Antibody (Cat# AAA266400)
Full Name
Recombinant Anti-TRIM25 Rabbit mAb
Gene Names
TRIM25; EFP; Z147; RNF147; ZNF147
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of hnRNP E2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
hnRNP E2, Monoclonal Recombinant Antibody (Cat# AAA266426)
Full Name
Recombinant Anti-hnRNP E2 Rabbit mAb
Gene Names
PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Ubiquitin (Linkage specific-K63) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Ubiquitin, Monoclonal Recombinant Antibody (Cat# AAA266449)
Full Name
Recombinant Anti-Ubiquitin (Linkage specific-K63) Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from serum-starved SkBr3, treated with EGF (200ng/mL, 10 min), using Phospho-HER2/ErbB2 (Tyr1221, 1222) Antibody, Rabbit Mab (#AAA259501)(Sino Biological) and other brands' antibodies (Company C) at dilution of 1:300000, 1:100000 and 1:10000.(Validation Experiment))
HER2/ErbB2, Monoclonal Recombinant Antibody (Cat# AAA259501)
Full Name
Phospho-HER2/ErbB2 (Tyr1221,1222) Antibody, Rabbit MAb
Reactivity
Human
Applications
Western Blot
Purity
Protein A
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279089 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
RELB, Monoclonal Recombinant Antibody (Cat# AAA279089)
Full Name
RELB Recombinant Monoclonal Antibody
Gene Names
RELB; IREL; I-REL; REL-B
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing K562 cells stained with AAA279130 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
TPSB2, Monoclonal Recombinant Antibody (Cat# AAA279130)
Full Name
TPSB2 Recombinant Monoclonal Antibody
Gene Names
TPSAB1; TPS1; TPS2; TPSB1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Human DLL3 with Anti-DLL3 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human DLL3 (CSB-MP882142HU2d7) at 2 ug/mL can bind Anti-DLL3 recombinant antibody. The EC50 is 3.990-4.723 ng/mL.)
IL2RG, Monoclonal Recombinant Antibody (Cat# AAA279309)
Full Name
IL2RG Recombinant Monoclonal Antibody
Gene Names
IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
Reactivity
Human, Cynomolgus
Applications
ELISA
Purity
Affinity-chromatography
Pricing
Bioactivity
(The Binding Activity of Mouse Tnfsf15 with Anti-TNFSF15 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Tnfsf15 at 2ug/mL can bind Anti-TNFSF15 recombinant antibody. The EC50 is 1.671-2.506ng/mL.)
TNFSF15, Monoclonal Recombinant Antibody (Cat# AAA279347)
Full Name
TNFSF15 Recombinant Monoclonal Antibody
Gene Names
TNFSF15; TL1; TL1A; VEGI; VEGI192A
Reactivity
Human, Cynomolgus, Mouse
Applications
ELISA
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA279177 (red line) at 1:300. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,018 events was performed.)
CALR, Monoclonal Recombinant Antibody (Cat# AAA279177)
Full Name
CALR Recombinant Monoclonal Antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Human CEACAM6 with Anti- CEACAM5/CEACAM6 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM6 at 2 ug/mL can bind Anti- CEACAM5/CEACAM6 recombinant antibody, the EC50 is 0.9430-1.377 ng/mL.)
CEACAM5/CEACAM6, Monoclonal Recombinant Antibody (Cat# AAA279028)
Full Name
CEACAM5/CEACAM6 Recombinant Monoclonal Antibody
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Mus musculus (Mouse)
Applications
ELISA
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC3 cells stained with AAA278985 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ARG2, Monoclonal Recombinant Antibody (Cat# AAA278985)
Full Name
ARG2 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278986 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FADS1, Monoclonal Recombinant Antibody (Cat# AAA278986)
Full Name
FADS1 Recombinant Monoclonal Antibody
Gene Names
FADS1; D5D; TU12; FADS6; FADSD5; LLCDL1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278990 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ISG15, Monoclonal Recombinant Antibody (Cat# AAA278990)
Full Name
ISG15 Recombinant Monoclonal Antibody
Gene Names
ISG15; G1P2; IP17; UCRP; IFI15; hUCRP
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PSMB8 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
PSMB8, Monoclonal Recombinant Antibody (Cat# AAA266366)
Full Name
Recombinant Anti-PSMB8 Rabbit mAb
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of E2F1 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
E2F1, Monoclonal Recombinant Antibody (Cat# AAA266367)
Full Name
Recombinant Anti-E2F1 Rabbit mAb
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of LCAT staining in human Brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
LCAT, Monoclonal Recombinant Antibody (Cat# AAA266382)
Full Name
Recombinant Anti-LCAT Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SKP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
SKP1, Monoclonal Recombinant Antibody (Cat# AAA266384)
Full Name
Recombinant Anti-SKP1 Rabbit mAb
Gene Names
SKP1; OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
PTEN, Monoclonal Recombinant Antibody (Cat# AAA266258)
Full Name
Recombinant Anti-PTEN Rabbit mAb
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
What are recombinant antibodies?
Recombinant antibodies are produced in the lab by utilizing a known genetic sequence (an amino acid and nucleotide sequence). This allows for high consistency in how each new lot will perform and how specific they will be for the target, regardless of how many times they are produced/reproduced.
They help solve the problems observed when traditional non-recombinant techniques are utilized, such as changes in cell behavior over time, differences between production batches, and accuracy in complex biological systems.
A powerful feature of recombinant antibodies is that they can also be modified through progressive genetic engineering and subsequent testing in order to make them perform better and/or more reliably.
Applications For Recombinant Antibodies
1. Basic Research
Recombinant antibodies are primarily utilized in laboratory experiments to study the function of various proteins in cells. They are helpful in finding and tracking specific molecules through their use in techniques such as ELISA, Western blotting, immunofluorescence, and flow cytometry.
2. Diagnostics
They are also key components in diagnostic tests used to detect diseases and infections. As recombinant antibodies are produced in a consistent and controlled way, they are able to provide highly reliable results across batches. Though recombinant antibodies are indeed quite useful in diagnostic applications, all of the products listed in AAA Biotech’s also known as AAA Bio or AAABio catalog are strictly for research-use only (RUO).
3. Therapeutics (Medical Treatments)
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
- Chimeric Antibodies: Commonly used in therapeutic treatment. They combine different pieces of mouse and human antibodies in order to reduce immune reactions.
- Humanized Antibodies: Modified non-human antibodies that are made to look the same as actual native human antibodies, making them safer and more effective.
- Antibody Fragments (Fab, scFv, VHH): Smaller pieces of antibody fragments that can reach deep into tissues and tumors. They are useful in cancer treatments and imaging.
- Bispecific Antibodies: Specially-engineered antibodies that can bind to two different target molecules at once. Each antibody has two “arms” that allow for two places where the target antigen can bind (both “arms” will recognize the same exact epitope). These bispecific antibodies are engineered so that each arm can detect a completely different epitope than the other. Used in many of the treatments for cancer, diabetes, Alzheimer’s disease, and more.
- Fc-Fusion Proteins: Used as drugs and research tools. They combine parts of antibodies with other proteins to improve stability and effectiveness.
4. Imaging and Detection
Small antibody fragments are also used as imaging agents in medical scans. Their small size helps them reach and report on the status of tumors quickly.
5. Drug Development and Delivery
Recombinant antibodies can also be designed and made to deliver drugs directly to diseased cells, especially in targeted cancer therapy.
6. Immunotherapy
These antibodies can aid the immune system recognize and fight diseases more effectively, which is often their role in cancer treatment.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Advantages of Recombinant Antibodies
- Reliable Results Every Time: Recombinant antibodies are exceptionally consistent from batch to batch. This means researchers can trust that results will remain the same each time they purchase and use them.
- More Specific and Accurate: Compared to polyclonal antibodies, recombinant antibodies are significantly less likely to exhibit non-specific binding, which promotes more accurate and clearer results.
- Easier to Develop: Making recombinant antibodies is simpler and quicker than the traditional method of producing monoclonal antibodies via hybridomas.
- No Genetic Drift: Over time, hybridoma cells can change or become unstable, which can result in the antibodies progressively losing their function/specificity. Recombinant antibodies avoid this problem because they are produced from a fixed template DNA sequence.
- Truly Monoclonal: Studies have shown that many hybridomas thought to be monoclonal actually are not so, which can lead to, in some cases, unreliable results. Recombinant antibodies are made using precise genetic sequence templates, ensuring they are truly specific to one target.
- Long-Term Stability and Safety: Since their amino acid and genetic sequences are known and stored, recombinant antibodies can be reproduced at any time with the same quality. This method protects against contamination, genetic changes over time, or even accidental loss/deterioration of cell lines.
Why Buy Recombinant Antibody from AAA Biotech?
- Highly Validated: The vast majority of our antibodies are thoroughly tested to ensure they work reliably across different applications and experimental platforms.
- Versatile Applications: Our antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).
- Support for Rare Species: We offer antibodies specifically designed for rare or less commonly used laboratory species — something many other suppliers do not provide.
- Wide Selection of Conjugated and Recombinant Forms: Choose from various labeled (conjugated) options and browse our high-quality recombinant antibodies – we are confident that we will have materials to meet your research needs.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
FAQ
1. What are recombinant antibodies used for?
Research (e.g., ELISA, Western blot, flow cytometry)
Disease diagnostics
Therapeutic treatments (e.g., cancer, autoimmune diseases)
Medical imaging
Targeted drug delivery
Vaccine development
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
2. What is the difference between traditional and recombinant antibodies?
Traditional antibodies: Produced using animal hosts (hybridomas are typically made from an original animal inoculation), slower, less consistent, risk of genetic drift
Recombinant antibodies: Produced using DNA template sequences via laboratory cell lines, faster, highly consistent, animal-free, easily customizable
3. What are antibodies used to treat?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Antibodies are proteins that your body makes to protect you from infections, allergies, and other potentially harmful substances. Scientists have used the intrinsic properties of antibodies to develop them into tools for scientific advancement, and can now create special lab-grown antibodies that aid in the treatment of diseases such as cancer, heart-related issues, and arthritis.
4. Why do we need recombinant proteins?
Recombinant proteins are lab-made proteins that have become essential in the advancement of science and medicine. They’re used in the lab for innumerable purposes, such as to help grow and support cells, especially for tasks like expanding cell colonies, helping them develop into other types, or turning them into stem cells.