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▼Recombinant Antibodies
Also available for purchase in our catalog, we have flexible recombinant antibodies that are excellent tools in many different types of experiments, and well-suited for those that are large-scale or long-term. Our collection of recombinant antibodies includes different forms/formats of said antibodies, such as labeled or purified versions, and can be made utilizing different species’ isotypes to suit various experimental requirements.
Viewing 350-400 of 3337 product results
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279063 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
USP22, Monoclonal Recombinant Antibody (Cat# AAA279063)
Full Name
USP22 Recombinant Monoclonal Antibody
Gene Names
USP22; USP3L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279080 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
PANX1, Monoclonal Recombinant Antibody (Cat# AAA279080)
Full Name
PANX1 Recombinant Monoclonal Antibody
Gene Names
PANX1; PX1; MRS1; UNQ2529
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of AK2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
AK2, Monoclonal Recombinant Antibody (Cat# AAA266272)
Full Name
Recombinant Anti-AK2 Rabbit mAb
Gene Names
AK2; ADK2; AK 2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of BAF155 staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
BAF155, Monoclonal Recombinant Antibody (Cat# AAA266283)
Full Name
Recombinant Anti-BAF155 Rabbit mAb
Gene Names
SMARCC1; Rsc8; SRG3; SWI3; BAF155; CRACC1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ALB staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
ALB, Monoclonal Recombinant Antibody (Cat# AAA266305)
Full Name
Recombinant Anti-ALB Rabbit mAb
Gene Names
ALB; PRO0883; PRO0903; PRO1341
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SNRPA1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
SNRPA1, Monoclonal Recombinant Antibody (Cat# AAA266318)
Full Name
Recombinant Anti-SNRPA1 Rabbit mAb
Gene Names
SNRPA1; Lea1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MSI2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
MSI2, Monoclonal Recombinant Antibody (Cat# AAA266331)
Full Name
Recombinant Anti-MSI2 Rabbit mAb
Gene Names
MSI2; MSI2H
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of IgG4 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IgG4, Monoclonal Recombinant Antibody (Cat# AAA266343)
Full Name
Recombinant Anti-IgG4 Rabbit mAb
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of IBA1 staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IBA1, Monoclonal Recombinant Antibody (Cat# AAA266397)
Full Name
Recombinant Anti-IBA1 Rabbit mAb
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Gephyrin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
Gephyrin, Monoclonal Recombinant Antibody (Cat# AAA266440)
Full Name
Recombinant Anti-Gephyrin Rabbit mAb
Gene Names
GPHN; GPH; GEPH; HKPX1; GPHRYN; MOCODC
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Cytokeratin 18 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Cytokeratin 18, Monoclonal Recombinant Antibody (Cat# AAA266240)
Full Name
Recombinant Anti-Cytokeratin 18 Rabbit mAb
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Caldesmon staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Caldesmon, Monoclonal Recombinant Antibody (Cat# AAA266254)
Full Name
Recombinant Anti-Caldesmon Rabbit mAb
Gene Names
CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279324 diluted at 1?100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.)
CSF3R, Monoclonal Recombinant Antibody (Cat# AAA279324)
Full Name
CSF3R Recombinant Monoclonal Antibody
Gene Names
CSF3R; CD114; GCSFR
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-chromatography
Pricing
Bioactivity
(The Binding Activity of Human CD79A&CD79B with Anti-CD79A recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human CD79A&CD79B at 2ug/mL can bind Anti-CD79A recombinant antibody. The EC50 is 8.012-9.339ng/mL.)
CD79A, Monoclonal Recombinant Antibody (Cat# AAA279339)
Full Name
CD79A Recombinant Monoclonal Antibody
Gene Names
CD79A; IGA; MB-1
Reactivity
Human
Applications
ELISA
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Untransfected HEK293T cells (green line) and transfected Human TSHR HEK293T stable cells (red line) were stained with anti-TSHR antibody (AAA279348) (2ug/1*106cells), washed and then followed by APC-conjugated anti-Human IgG FC/FACS antibody and analyzed with flow cytometry.)
TSHR, Monoclonal Recombinant Antibody (Cat# AAA279348)
Full Name
TSHR Recombinant Monoclonal Antibody
Gene Names
TSHR; LGR3; CHNG1; hTSHR-I
Reactivity
Human
Applications
ELISA
Purity
Affinity chromatography
Pricing
IHC (Immunohistochemisry)
(IHC image of AAA278975 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
FIS1, Monoclonal Recombinant Antibody (Cat# AAA278975)
Full Name
FIS1 Recombinant Monoclonal Antibody
Gene Names
FIS1; TTC11; CGI-135
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with AAA279001 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
PAH, Monoclonal Recombinant Antibody (Cat# AAA279001)
Full Name
PAH Recombinant Monoclonal Antibody
Gene Names
PAH; PH; PKU; PKU1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA279306MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD276, Monoclonal Recombinant Antibody (Cat# AAA279306)
Full Name
CD276 Recombinant Monoclonal Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human, Cynomolgus
Applications
ELISA
Purity
Affinity-chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279120 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
MKI67, Monoclonal Recombinant Antibody (Cat# AAA279120)
Full Name
MKI67 Recombinant Monoclonal Antibody
Gene Names
MKI67; KIA
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279144 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ATP7B, Monoclonal Recombinant Antibody (Cat# AAA279144)
Full Name
ATP7B Recombinant Monoclonal Antibody
Gene Names
ATP7B; WD; PWD; WC1; WND
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC-3 cells stained with AAA279154 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.)
HIST1H4A, Monoclonal Recombinant Antibody (Cat# AAA279154)
Full Name
HIST1H4A Recombinant Monoclonal Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279004 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
NTHL1, Monoclonal Recombinant Antibody (Cat# AAA279004)
Full Name
NTHL1 Recombinant Monoclonal Antibody
Gene Names
NTHL1; NTH1; OCTS3; hNTH1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Mouse Prlr with Anti-PRLR recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Prlr at 5 ug/mL can bind Anti-PRLR recombinant antibody, the EC50 is 4.021-8.706 ng/mL.)
PRLR, Monoclonal Recombinant Antibody (Cat# AAA279019)
Full Name
PRLR Recombinant Monoclonal Antibody
Gene Names
PRLR; hPRLrI
Reactivity
Human, Mouse
Applications
ELISA
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279048 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
NCAM, Monoclonal Recombinant Antibody (Cat# AAA279048)
Full Name
NCAM Recombinant Monoclonal Antibody
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Monkeypox virus (strain Zaire-96-I-16)
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of eIF2 alpha staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
eIF2 alpha, Monoclonal Recombinant Antibody (Cat# AAA266420)
Full Name
Recombinant Anti-eIF2 alpha Rabbit mAb
Gene Names
EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of IDH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IDH1, Monoclonal Recombinant Antibody (Cat# AAA266437)
Full Name
Recombinant Anti-IDH1 Rabbit mAb
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of TARBP2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
TARBP2, Monoclonal Recombinant Antibody (Cat# AAA266443)
Full Name
Recombinant Anti-TARBP2 Rabbit mAb
Gene Names
TARBP2; LOQS; TRBP; TRBP1; TRBP2
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of CALU staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
CALU, Monoclonal Recombinant Antibody (Cat# AAA266376)
Full Name
Recombinant Anti-CALU Rabbit mAb
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of UQCRH staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
UQCRH, Monoclonal Recombinant Antibody (Cat# AAA266401)
Full Name
Recombinant Anti-UQCRH Rabbit mAb
Gene Names
UQCRH; QCR6; UQCR8
Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of EGLN2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
EGLN2, Monoclonal Recombinant Antibody (Cat# AAA266408)
Full Name
Recombinant Anti-EGLN2 Rabbit mAb
Gene Names
EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of CCR8 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
CCR8, Monoclonal Recombinant Antibody (Cat# AAA266412)
Full Name
Recombinant Anti-CCR8 Rabbit mAb
Gene Names
CCR8; CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of Beta-actin expression in wild type (WT) and knockdown (KD) HeLa cell lysates.)
Beta-actin, Monoclonal Recombinant Antibody (Cat# AAA266249)
Full Name
Recombinant Anti-Beta-actin Rabbit mAb [KO/KD Validated]
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of BAG6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
BAG6, Monoclonal Recombinant Antibody (Cat# AAA266270)
Full Name
Recombinant Anti-BAG6 Rabbit mAb
Gene Names
BAG6; G3; BAT3; BAG-6; D6S52E
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Vimentin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
Vimentin, Monoclonal Recombinant Antibody (Cat# AAA266313)
Full Name
Recombinant Anti-Vimentin Rabbit mAb
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Smooth Muscle Actin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Smooth Muscle Actin, Monoclonal Recombinant Antibody (Cat# AAA266332)
Full Name
Recombinant Anti-Smooth Muscle Actin Rabbit mAb
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279327 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SLC1A5, Monoclonal Recombinant Antibody (Cat# AAA279327)
Full Name
SLC1A5 Recombinant Monoclonal Antibody
Gene Names
SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity-chromatography
Pricing
Bioactivity
(The Binding Activity of Human PTK7 with Anti-PTK7 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human PTK7 at 2ug/mL can bind Anti-PTK7 recombinant antibody. The EC50 is 0.3675 - 0.6584ng/mL.)
PTK7, Monoclonal Recombinant Antibody (Cat# AAA279350)
Full Name
PTK7 Recombinant Monoclonal Antibody
Gene Names
PTK7; CCK4; CCK-4
Reactivity
Human, Macaca fascicularis
Applications
ELISA
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA279174 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,015 events was performed.)
CTLA4, Monoclonal Recombinant Antibody (Cat# AAA279174)
Full Name
CTLA4 Recombinant Monoclonal Antibody
Gene Names
CTLA4; CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Jurkat cells stained with AAA279107 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
AKT1/2/3, Monoclonal Recombinant Antibody (Cat# AAA279107)
Full Name
AKT1/2/3 Recombinant Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA278965 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
E2F4, Monoclonal Recombinant Antibody (Cat# AAA278965)
Full Name
E2F4 Recombinant Monoclonal Antibody
Gene Names
E2F4; E2F-4
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA278978 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
COPS3, Monoclonal Recombinant Antibody (Cat# AAA278978)
Full Name
COPS3 Recombinant Monoclonal Antibody
Gene Names
COPS3; CSN3; SGN3
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279054 diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.)
HIST1H3A, Monoclonal Recombinant Antibody (Cat# AAA279054)
Full Name
Formyl-HIST1H3A (K122) Recombinant Monoclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279057 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
MAPT, Monoclonal Recombinant Antibody (Cat# AAA279057)
Full Name
Phospho-MAPT (S199) Recombinant Monoclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279060 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GLA, Monoclonal Recombinant Antibody (Cat# AAA279060)
Full Name
GLA Recombinant Monoclonal Antibody
Gene Names
GLA; GALA
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
IHC (Immunohistochemisry)
(IHC image of AAA279064 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.)
CASP3, Monoclonal Recombinant Antibody (Cat# AAA279064)
Full Name
CASP3 Recombinant Monoclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279076 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
LGR6, Monoclonal Recombinant Antibody (Cat# AAA279076)
Full Name
LGR6 Recombinant Monoclonal Antibody
Gene Names
LGR6; GPCR; VTS20631
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279002 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
NSDHL, Monoclonal Recombinant Antibody (Cat# AAA279002)
Full Name
NSDHL Recombinant Monoclonal Antibody
Gene Names
NSDHL; H105E3; XAP104; SDR31E1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
WB (Western Blot)
(Anti-Carboxypeptidase A monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human Carboxypeptidase A Protein 100ngSecondaryRabbit Anti-Mouse IgG1/HRP(#AAA259413-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions.)
IgG1, Monoclonal Recombinant Antibody (Cat# AAA259413)
Full Name
Rabbit Anti-Mouse IgG1 Secondary Antibody, Rabbit MAb
Reactivity
Mouse
Applications
ELISA
Purity
Protein A
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of SARS-COV-2 Spike (YP_009724390.1, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Spike Chimera Mab,then a FITC-conjugated second step antibody.(Validation Experiment))
SARS-CoV/SARS-CoV-2 Spike, Monoclonal Recombinant Antibody (Cat# AAA259163)
Full Name
SARS-CoV/SARS-CoV-2 Spike antibody,Chimeric Mab
Reactivity
SARS-CoV-2
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Protein A
Pricing
WB (Western Blot)
(Anti-IL-3R alpha/CD123 monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human IL-3R alpha/CD123 Protein 100ngSecondaryRabbit Anti-Mouse IgG2b/HRP(#AAA259473-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions. (Validation Experiment))
IgG2b, Monoclonal Recombinant Antibody (Cat# AAA259473)
Full Name
Rabbit Anti-Mouse IgG2b Secondary Antibody, Rabbit MAb
Reactivity
Mouse
Applications
ELISA
Purity
Protein A
Pricing
What are recombinant antibodies?
Recombinant antibodies are produced in the lab by utilizing a known genetic sequence (an amino acid and nucleotide sequence). This allows for high consistency in how each new lot will perform and how specific they will be for the target, regardless of how many times they are produced/reproduced.
They help solve the problems observed when traditional non-recombinant techniques are utilized, such as changes in cell behavior over time, differences between production batches, and accuracy in complex biological systems.
A powerful feature of recombinant antibodies is that they can also be modified through progressive genetic engineering and subsequent testing in order to make them perform better and/or more reliably.
Applications For Recombinant Antibodies
1. Basic Research
Recombinant antibodies are primarily utilized in laboratory experiments to study the function of various proteins in cells. They are helpful in finding and tracking specific molecules through their use in techniques such as ELISA, Western blotting, immunofluorescence, and flow cytometry.
2. Diagnostics
They are also key components in diagnostic tests used to detect diseases and infections. As recombinant antibodies are produced in a consistent and controlled way, they are able to provide highly reliable results across batches. Though recombinant antibodies are indeed quite useful in diagnostic applications, all of the products listed in AAA Biotech’s also known as AAA Bio or AAABio catalog are strictly for research-use only (RUO).
3. Therapeutics (Medical Treatments)
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
- Chimeric Antibodies: Commonly used in therapeutic treatment. They combine different pieces of mouse and human antibodies in order to reduce immune reactions.
- Humanized Antibodies: Modified non-human antibodies that are made to look the same as actual native human antibodies, making them safer and more effective.
- Antibody Fragments (Fab, scFv, VHH): Smaller pieces of antibody fragments that can reach deep into tissues and tumors. They are useful in cancer treatments and imaging.
- Bispecific Antibodies: Specially-engineered antibodies that can bind to two different target molecules at once. Each antibody has two “arms” that allow for two places where the target antigen can bind (both “arms” will recognize the same exact epitope). These bispecific antibodies are engineered so that each arm can detect a completely different epitope than the other. Used in many of the treatments for cancer, diabetes, Alzheimer’s disease, and more.
- Fc-Fusion Proteins: Used as drugs and research tools. They combine parts of antibodies with other proteins to improve stability and effectiveness.
4. Imaging and Detection
Small antibody fragments are also used as imaging agents in medical scans. Their small size helps them reach and report on the status of tumors quickly.
5. Drug Development and Delivery
Recombinant antibodies can also be designed and made to deliver drugs directly to diseased cells, especially in targeted cancer therapy.
6. Immunotherapy
These antibodies can aid the immune system recognize and fight diseases more effectively, which is often their role in cancer treatment.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Advantages of Recombinant Antibodies
- Reliable Results Every Time: Recombinant antibodies are exceptionally consistent from batch to batch. This means researchers can trust that results will remain the same each time they purchase and use them.
- More Specific and Accurate: Compared to polyclonal antibodies, recombinant antibodies are significantly less likely to exhibit non-specific binding, which promotes more accurate and clearer results.
- Easier to Develop: Making recombinant antibodies is simpler and quicker than the traditional method of producing monoclonal antibodies via hybridomas.
- No Genetic Drift: Over time, hybridoma cells can change or become unstable, which can result in the antibodies progressively losing their function/specificity. Recombinant antibodies avoid this problem because they are produced from a fixed template DNA sequence.
- Truly Monoclonal: Studies have shown that many hybridomas thought to be monoclonal actually are not so, which can lead to, in some cases, unreliable results. Recombinant antibodies are made using precise genetic sequence templates, ensuring they are truly specific to one target.
- Long-Term Stability and Safety: Since their amino acid and genetic sequences are known and stored, recombinant antibodies can be reproduced at any time with the same quality. This method protects against contamination, genetic changes over time, or even accidental loss/deterioration of cell lines.
Why Buy Recombinant Antibody from AAA Biotech?
- Highly Validated: The vast majority of our antibodies are thoroughly tested to ensure they work reliably across different applications and experimental platforms.
- Versatile Applications: Our antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).
- Support for Rare Species: We offer antibodies specifically designed for rare or less commonly used laboratory species — something many other suppliers do not provide.
- Wide Selection of Conjugated and Recombinant Forms: Choose from various labeled (conjugated) options and browse our high-quality recombinant antibodies – we are confident that we will have materials to meet your research needs.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
FAQ
1. What are recombinant antibodies used for?
Research (e.g., ELISA, Western blot, flow cytometry)
Disease diagnostics
Therapeutic treatments (e.g., cancer, autoimmune diseases)
Medical imaging
Targeted drug delivery
Vaccine development
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
2. What is the difference between traditional and recombinant antibodies?
Traditional antibodies: Produced using animal hosts (hybridomas are typically made from an original animal inoculation), slower, less consistent, risk of genetic drift
Recombinant antibodies: Produced using DNA template sequences via laboratory cell lines, faster, highly consistent, animal-free, easily customizable
3. What are antibodies used to treat?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Antibodies are proteins that your body makes to protect you from infections, allergies, and other potentially harmful substances. Scientists have used the intrinsic properties of antibodies to develop them into tools for scientific advancement, and can now create special lab-grown antibodies that aid in the treatment of diseases such as cancer, heart-related issues, and arthritis.
4. Why do we need recombinant proteins?
Recombinant proteins are lab-made proteins that have become essential in the advancement of science and medicine. They’re used in the lab for innumerable purposes, such as to help grow and support cells, especially for tasks like expanding cell colonies, helping them develop into other types, or turning them into stem cells.