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10000 results for "Recombinant Proteins" - showing 5700-5750
Application Data
(Anti-SynCAM Picoband antibody, AAA46044-2.jpgAll lanes: Anti SYNCAM (AAA46044) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 60KDObserved bind size: 60KD)
Cell adhesion molecule 1, Polyclonal Antibody (Cat# AAA46044)
Full Name
Anti-SynCAM Antibody
Gene Names
CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of AK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
AK2, Monoclonal Antibody (Cat# AAA48206)
Full Name
AK2 antibody
Gene Names
AK2; ADK2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of MYL2 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYL2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
MYL2, Monoclonal Antibody (Cat# AAA48057)
Full Name
MYL2 antibody
Gene Names
MYL2; MLC2; CMH10; MLC-2s/v
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
Application Data
(Recombinant SMYD2 protein activity assay 2 ug Calf Thymus Octamers was incubated with 0.5 ug and 1 ug SMYD2 protein respectivley in reaction buffer containing radioactive 3H-SAM for 3 hours at room temperature. Activity was detected by Autoradiography. *Our result showed that H4 may also be methylated by SMYD2.)
SMYD2, Recombinant Protein (Cat# AAA59718)
Full Name
Recombinant SMYD2 protein
Gene Names
SMYD2; KMT3C; HSKM-B; ZMYND14
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for Recombinant SETDB1 protein activity. 3 uM histone peptide H3 (1-21) was incubated with SETDB1 in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.)
WB (Western Blot)
(The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human Hexokinase1 protein Lane 2.: Recombinant human Hexokinase2 protein Lane 3.: Recombinant human Hexokinase3 protein Lane 4.: Recombinant human Hexokinase4 protein)
Hexokinase, Monoclonal Antibody (Cat# AAA48339)
Full Name
Hexokinase
Gene Names
HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of PGM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
PGM1, Monoclonal Antibody (Cat# AAA48217)
Full Name
PGM1 antibody
Gene Names
PGM1; CDG1T; GSD14
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
CKB, Monoclonal Antibody (Cat# AAA48237)
Full Name
CKB antibody
Gene Names
CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant CLPP protein using CLPP antibody)
CLPP, Monoclonal Antibody (Cat# AAA106414)
Full Name
CLPP antibody
Gene Names
CLPP; DFNB81; PRLTS3
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
CLPP antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SH2B3 protein using SH2B3 antibody)
SH2B3, Monoclonal Antibody (Cat# AAA107502)
Full Name
SH2B3 antibody
Gene Names
SH2B3; LNK; IDDM20
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
SH2B3 antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mononucleosomes H3K4me1 (EPL) - biotinylated. Unmodified mononucleosomes (Lane 1) and Mononucleosomes H3K4me1 (Lane 2) were detected with anti-H3K4me1 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Mononucleosomes H3K4me1 can be detected by anti- H3K4me1 antibody. Nucleosomes = Mononucleosomes)
Mononucleosomes H3K4me1, Recombinant Protein (Cat# AAA59764)
Full Name
Recombinant Mononucleosomes H3K4me1 (EPL)-biotinylated
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
IP (Immunoprecipitation)
(ANPEP was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ANPEP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ANPEP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 140 kDaObserved band size :180 kDa)
CD13, Polyclonal Antibody (Cat# AAA254267)
Full Name
Anti-CD13 Antibody, Rabbit Polyclonal
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
SDS-PAGE
(SDS-PAGE)
Signaling Lymphocytic Activation Molecule Family, Member 5 (SLAMF5), Recombinant Protein (Cat# AAA152186)
Full Name
Recombinant Signaling Lymphocytic Activation Molecule Family, Member 5 (SLAMF5)
Gene Names
CD84; LY9B; hCD84; mCD84; SLAMF5
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
ELISA
(Biotinylated Human SIRP alpha V4 Protein ELISA DataImmobilized Human CD47, hFc Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.47?g/ml determined by ELISA.)
SIRP alpha V4, Recombinant Protein (Cat# AAA76349)
Full Name
Biotinylated Human SIRP alpha V4 Protein Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
(ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseinalphasynucleinmonomersandfibrils.Uponbinding,theemissionspectrumofthedyeexperiencesared-shift,andincreasedfluorescenceintensity.TheRatAlphaSynucleinmonomer isveryactiveandwasabletoformmorebeta-sheetstructurealonethanwiththecombinationofmonomerandfibril.Incombination,thefibril(AAA254099)isthemajorityoftheseed.)
Alpha Synuclein, Recombinant Protein (Cat# AAA254099)
Full Name
Rat Recombinant Alpha Synuclein Protein Pre-formed Fibrils
Applications
Western Blot
Purity
>95%; Ion-exchange Purified
Pricing
Amino Acid Info
(Amino Acid info)
Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked (aTRX), Recombinant Protein (Cat# AAA153696)
Full Name
Recombinant Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked (aTRX)
Gene Names
ATRX; JMS; SHS; XH2; XNP; ATR2; SFM1; RAD54; MRXHF1; RAD54L; ZNF-HX
Applications
Western Blot
Purity
Greater than 80% by SDS-PAGE
Pricing
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K18I) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added to 60 &mixeo;l 2×SDS loading buffer and boiled for 10 min at 95°&C. 2.4 micro;l samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel shows that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60217)
Full Name
Recombinant Mononucleosomes H3.3 (K18I)-biotin
Pricing
Application Data
(Recombinant Mononucleosomes H3.3 (K4I) DNA gel Recombinant Mononucleosomes H3.3 (K4I) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K4I). Intact mononucleosomes H3.3 (K4I) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60229)
Full Name
Recombinant Mononucleosomes H3.3 (K4I)
Pricing
Application Data
(HTRF assay for JMJD3 / KDM6B (1043-1682) activity.1 uM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.)
JMJD3/KDM6B, Recombinant Protein (Cat# AAA59730)
Full Name
Recombinant JMJD3/KDM6B (1043-1682) protein
Gene Names
KDM6B; JMJD3
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ITM2B protein in paraffin embedded Human Kidney tissue using ITM2B antibody)
ITM2B, Monoclonal Antibody (Cat# AAA106915)
Full Name
ITM2B antibody
Gene Names
ITM2B; BRI; FBD; ABRI; BRI2; E25B; E3-16; RDGCA; imBRI2; BRICD2B
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ITM2B antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant KCNJ3 protein using KCNJ3 antibody)
KCNJ3, Monoclonal Antibody (Cat# AAA106606)
Full Name
KCNJ3 antibody
Gene Names
KCNJ3; KGA; GIRK1; KIR3.1
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
KCNJ3 antibody was purified by affinity chromatography.
Pricing
SDS-PAGE
Neuropilin 2 (NRP2), Recombinant Protein (Cat# AAA134618)
Full Name
Recombinant Neuropilin 2 (NRP2)
Reactivity
Rat
Applications
Western Blot
Purity
>98%
Pricing
Application Data
(Biotinylated Human CEACAM-6/CD66c Protein SPR DataHuman CEACAM-8, His Tag immobilized on CM5 Chip can bind Biotinylated Human CEACAM6, His-Avi Tag with an affinity constant of 3.81 ?M as determined in SPR assay (Biacore T200).)
CEACAM-6/CD66c, Recombinant Protein (Cat# AAA76247)
Full Name
Biotinylated Human CEACAM-6/CD66c Protein Recombinant
Gene Names
CEACAM6; NCA; CEAL; CD66c
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
SDS-PAGE
(SDS-PAGE)
Phospholipase A2 Group VII (LpPLA2), Recombinant Protein (Cat# AAA152254)
Full Name
Recombinant Phospholipase A2 Group VII (LpPLA2)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Applications
Western Blot
Purity
Greater than 80% by SDS-PAGE
Pricing
Amino Acid Info
Eukaryotic Fatty Acid Binding Protein 2, Intestinal (FABP2), Protein (Cat# AAA153175)
Full Name
Eukaryotic Fatty Acid Binding Protein 2, Intestinal (FABP2)
Gene Names
Fabp2; Fabpi; I-FABP
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2). Confirmation of Purity and Integrity of Antibody.)
RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9, Monoclonal Antibody (Cat# AAA215279)
Full Name
RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9
Gene Names
EBAG9; EB9; PDAF
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
EpCAM, Polyclonal Antibody (Cat# AAA255085)
Full Name
Anti-EpCAM Antibody, Rabbit Polyclonal
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
Amino Acid Info
Eukaryotic Cluster Of Differentiation 58 (CD58), Protein (Cat# AAA153191)
Full Name
Eukaryotic Cluster Of Differentiation 58 (CD58)
Gene Names
CD58; ag3; LFA3; LFA-3
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Heat Shock Protein Beta 8 (HSPb8), Recombinant Protein (Cat# AAA134569)
Full Name
Recombinant Heat Shock Protein Beta 8 (HSPb8)
Gene Names
HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 95%
Pricing
ELISA
(Biotinylated Human E-Selectin/CD62E Protein ELISA DataImmobilized Biotinylated Human E-Selectin at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.)
E-Selectin/CD62E, Recombinant Protein (Cat# AAA76283)
Full Name
Biotinylated Human E-Selectin/CD62E Protein Recombinant
Gene Names
SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
SDS-PAGE
(SDS-PAGE)
Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1), Recombinant Protein (Cat# AAA152241)
Full Name
Recombinant Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1)
Gene Names
KIR2DL1; NKAT; NKAT1; p58.1; CD158A; KIR221; NKAT-1; KIR-K64
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 17 (FGF17) is a member of the fibroblast growth factorfamily. FGF family members possess broad mitogenicand cell survival activities and are involved in a varietyof biological processes, including embryonicdevelopment cell growth, morphogenesis, tissue repair, tumor growth, andinvasion. Fibroblast Growth Factor Receptor 1 (FGFR1) is high affinity receptor for FGF17, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF17 and recombinant human FGFR1. Briefly, biotin-linked FGF17 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF17 and human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.52 ug/mL.)
Fibroblast Growth Factor 17 (FGF17), Active Protein (Cat# AAA161896)
Full Name
Active Fibroblast Growth Factor 17 (FGF17)
Gene Names
FGF17; HH20; FGF-13; FGF-17
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE Analysis of Purified CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/1495R).)
CD99/MIC2, Monoclonal Antibody (Cat# AAA214447)
Full Name
CD99/MIC2 (Ewing's Sarcoma Marker)
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry
Pricing
Application Data
(HTRF assay for recombinant AKT1 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT1 protein a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
AKT1, Recombinant Protein (Cat# AAA60154)
Full Name
Recombinant AKT1 protein
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Pricing
Amino Acid Info
Eukaryotic Catenin Delta 1 (CTNNd1), Protein (Cat# AAA153224)
Full Name
Eukaryotic Catenin Delta 1 (CTNNd1)
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of CD59 using anti-CD59 antibody (AAA124602).CD59 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD59 Antibody (AAA124602) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD59, Polyclonal Antibody (Cat# AAA124602)
Full Name
Anti-CD59 Picoband antibody
Gene Names
Cd59a; Cd59; AA987121; protectin
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
SARS Associated Coronavirus Spike, Active Protein (Cat# AAA38073)
Full Name
Recombinant SARS Associated Coronavirus Spike (14-1195 a.a.)
Gene Names
S; E2
Purity
Greater than 85.0% as determined by SDS-PAGE.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD1a-Monospecific Recombinant Rabbit Monoclonal Antibody (C1A/1506R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
CD1a/HTA1, Monoclonal Antibody (Cat# AAA214570)
Full Name
CD1a/HTA1 (Mature Langerhans Cells Marker)
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Reactivity
Human
Applications
Immunohistochemistry
Pricing
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant nucleosomes and histone octamers as substrates. 2 ug Recombinant mononucleosomes (H3.1) were incubated with 0 ug (-), 0.05 ug (+), 0.1 ug (++), 0.2 ug (+++) NSD2-SET protein, respectively, in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H3K36me2). The Western blot result shows that recombinant mononucleosomes (H3.1) are better substrate than histone octamer for NSD2-SET assay.)
Mononucleosomes, Recombinant Protein (Cat# AAA59701)
Full Name
Recombinant Mononucleosomes (H3.1)-biotinylated
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified CD59 Rabbit Recombinant Monoclonal Antibody (MACIF/2867R). Confirmation of Purity and Integrity of Antibody.)
CD59/Complement Regulatory Protein/Protectin, Monoclonal Antibody (Cat# AAA214854)
Full Name
CD59/Complement Regulatory Protein/Protectin
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human.
Does not react with baboon, horse. Others not tested.
Does not react with baboon, horse. Others not tested.
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R). Confirmation of Purity and Integrity of Antibody.)
HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA214390)
Full Name
HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey and Chicken.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry
Pricing
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Renin, Monoclonal Antibody (Cat# AAA255266)
Full Name
Recombinant Anti-Renin Antibody, Rabbit Monoclonal
Gene Names
REN; HNFJ2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Protein A
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of CKB using anti-CKB antibody (AAA124662).CKB was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CKB Antibody (AAA124662) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CKB, Polyclonal Antibody (Cat# AAA124662)
Full Name
Anti-CKB Picoband antibody
Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Skeletal muscle Tissue; Primary Ab: 20ug/ml Mouse AntiHuman ISLR AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR), Monoclonal Antibody (Cat# AAA151881)
Full Name
Monoclonal Antibody to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR)
Gene Names
ISLR; HsT17563
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
WB (Western Blot)
(Western Blot; Sample: Rat Serum Primary Ab: 4ug/ml Mouse AntiHuman CRP Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
C Reactive Protein (CRP), Monoclonal Antibody (Cat# AAA151638)
Full Name
Monoclonal Antibody to C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Reactivity
Human, Rat, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Progesterone Receptor Mouse Monoclonal Antibody (PGR/2694).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Progesterone Receptor, Monoclonal Antibody (Cat# AAA214792)
Full Name
Progesterone Receptor (Marker of Progestin Dependence)
Gene Names
PGR; PR; NR3C3
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of IGF2R using anti-IGF2R antibody (AAA125909).IGF2R was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-IGF2R Antibody (AAA125909) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IGF2R, Monoclonal Antibody (Cat# AAA125909)
Full Name
Anti-IGF2R Antibody (monoclonal, 6G2)
Gene Names
IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Serum Primary Ab: 3ug/ml Mouse AntiHuman LUM Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Lumican (LUM), Monoclonal Antibody (Cat# AAA151720)
Full Name
Monoclonal Antibody to Lumican (LUM)
Gene Names
LUM; LDC; SLRR2D
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody.Creatine Kinase MM was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Creatine Kinase MM antibody.Creatine Kinase MM was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Creatine Kinase MM Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Creatine Kinase MM/CKM, Polyclonal Antibody (Cat# AAA124953)
Full Name
Anti-Creatine Kinase MM/CKM Antibody
Gene Names
CKM; CKMM; M-CK; CPK-M
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Porcine Colon Tissue; Primary Ab: 10ug/ml Mouse AntiHuman PKCe AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Protein Kinase C Epsilon (PKCe), Monoclonal Antibody (Cat# AAA151538)
Full Name
Monoclonal Antibody to Protein Kinase C Epsilon (PKCe)
Gene Names
PRKCE; PKCE; nPKC-epsilon
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing