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10000 results for "Recombinant Proteins" - showing 7400-7450
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific CD23 Mouse Monoclonal Antibody (FCER2/3592). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
CD23 (Fc Epsilon RII), Monoclonal Antibody (Cat# AAA215479)
Full Name
CD23 (Fc Epsilon RII)
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab conjugated to Biotin
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of GRB10 using anti-GRB10 antibody (AAA125616).GRB10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GRB10 Antibody (AAA125616) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GRB10, Polyclonal Antibody (Cat# AAA125616)
Full Name
Anti-GRB10 Antibody
Gene Names
Grb10; Meg1; AI325020; mKIAA0207; 5730571D09Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of U87 cells using anti-GRB14 antibody (AAA125717).Overlay histogram showing U87 cells stained with AAA125717 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB14 Antibody (AAA125717,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GRB14, Polyclonal Antibody (Cat# AAA125717)
Full Name
Anti-GRB14 Antibody
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of A431 cells using anti-SHMT1 antibody (AAA125924).Overlay histogram showing A431 cells stained with AAA125924 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- SHMT1 Antibody (AAA125924, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SHMT1, Monoclonal Antibody (Cat# AAA125924)
Full Name
Anti-SHMT1 Antibody (monoclonal, 9C7)
Gene Names
SHMT1; SHMT; CSHMT
Reactivity
Human, Mouse, Rat, Monkey
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 5. IF analysis of AKAP12 using anti- AKAP12 antibody (AAA125648).AKAP12 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- AKAP12 Antibody (AAA125648) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
AKAP12, Polyclonal Antibody (Cat# AAA125648)
Full Name
Anti-AKAP12 Antibody
Gene Names
Akap12; Srcs5; SSeCKS; Tsga12; AI317366
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (AAA125585).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human T-47D whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human SW620 whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: rat ovary tissue lysatesLane 8: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # AAA125585) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 210KD.)
Integrin beta 4/ITGB4, Polyclonal Antibody (Cat# AAA125585)
Full Name
Anti-Integrin beta 4/ITGB4 Antibody
Gene Names
ITGB4; CD104
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of A431 cells using anti-STAT5A/B antibody (AAA127034).Overlay histogram showing A431 cells stained with AAA127034 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5A/B Antibody (AAA127034, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STAT5A/B, Polyclonal Antibody (Cat# AAA127034)
Full Name
Anti-STAT5A/B Antibody Picoband
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-HAO1 antibody (AAA127570).Overlay histogram showing SiHa cells stained with AAA127570 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAO1 Antibody (AAA127570, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HAO1, Polyclonal Antibody (Cat# AAA127570)
Full Name
Anti-HAO1 Antibody Picoband
Gene Names
HAO1; GOX; GOX1; HAOX1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot; Sample: Rat Heart lysate Primary Ab: 2ug/ml Mouse AntiHuman FABP4 Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Fatty Acid Binding Protein 4 (FABP4), Monoclonal Antibody (Cat# AAA151734)
Full Name
Monoclonal Antibody to Fatty Acid Binding Protein 4 (FABP4)
Gene Names
FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
Reactivity
Human, Rat, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
Bioactivity
(Carbonic anhydrase catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Carbonic Anhydrase VII encoded by the CA7 gene is a cytosolic protein predominantly expressed in the salivary gland. CA7 may have additional tissue distributions and functions. For example, studies with CA inhibitors provide evidence that human CA7 is the CA isozyme responsible for the anticonvulsant/antiepileptic activity of sulfonamides and sulfamates. In fact, CA7 has been shown in a rat model to act as a developmental switch in GABAergic signaling in neurons. The activity of recombinant mouse CA7 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA7 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase VII (CA7), Active Protein (Cat# AAA161904)
Full Name
Active Carbonic Anhydrase VII (CA7)
Gene Names
Car7; Ca7; AV343731
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-CRABP1 antibody (AAA127490).Overlay histogram showing SH-SY5Y cells stained with AAA127490 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRABP1 Antibody (AAA127490, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CRABP1, Polyclonal Antibody (Cat# AAA127490)
Full Name
Anti-CRABP1 Antibody Picoband
Gene Names
CRABP1; RBP5; CRABP; CRABPI; CRABP-I
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-Amphiphysin/AMPH antibody (AAA127110).Overlay histogram showing U87 cells stained with AAA127110 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Amphiphysin/AMPH Antibody (AAA127110, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Amphiphysin/AMPH, Polyclonal Antibody (Cat# AAA127110)
Full Name
Anti-Amphiphysin/AMPH Antibody Picoband
Gene Names
AMPH; AMPH1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HEL cells using anti-HTRA2 antibody (AAA127088).Overlay histogram showing HEL cells stained with AAA127088 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HTRA2 Antibody (AAA127088, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HTRA2, Polyclonal Antibody (Cat# AAA127088)
Full Name
Anti-HTRA2 Antibody Picoband
Gene Names
HTRA2; OMI; PARK13; PRSS25
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-PKC Alpha/PRKCA antibody (AAA127009).Overlay histogram showing SiHa cells stained with AAA127009 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC Alpha/PRKCA Antibody (AAA127009, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PKC Alpha/PRKCA, Polyclonal Antibody (Cat# AAA127009)
Full Name
Anti-PKC Alpha/PRKCA Antibody Picoband
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-Serine racemase/SRR antibody (AAA127133).Overlay histogram showing U87 cells stained with AAA127133 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serine racemase/SRR Antibody (AAA127133, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Serine racemase/SRR, Polyclonal Antibody (Cat# AAA127133)
Full Name
Anti-Serine racemase/SRR Antibody Picoband
Gene Names
SRR; ILV1; ISO1
Reactivity
Human, Monkey
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
ICC (Immunocytochemistry)
(Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20? and using anti-CSK mouse mAb (dilution 1:50).)
CSK, Monoclonal Antibody (Cat# AAA320344)
Full Name
CSK Mouse mAb
Reactivity
Human, Rat
Applications
Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western blot detection of cIAP2 in Ramos.COS7.Raji and HeLa cell lysates using cIAP2 antibody(1:1000 diluted). Exposion time: 5min. Predicted band size: 68KDa.Observed band size:72KDa.)
cIAP2, Monoclonal Antibody (Cat# AAA320355)
Full Name
cIAP2 Mouse mAb
Gene Names
BIRC3; AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; MALT2; RNF49; c-IAP2
Reactivity
Human, Monkey
Applications
Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing
IP (Immunoprecipitation)
(B2M was immunoprecipitated using:; Lane A:0.5 mg A-431 Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; Lane C:0.5 mg HL-60 Whole Cell Lysate; 4 uL anti- B2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- B2M rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 14kDa; Observed band size :12 kDa)
Beta-2 microglobulin, Polyclonal Antibody (Cat# AAA258634)
Full Name
Beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
Interferon Gamma Receptor 2 (IFNgR2), Active Protein (Cat# AAA161936)
Full Name
Active Interferon Gamma Receptor 2 (IFNgR2)
Gene Names
IFNGR2; AF-1; IFGR2; IFNGT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of NUP133 using anti-NUP133 antibody (AAA127290) and anti-Beta Tubulin antibody (M01857-3).NUP133 was detected in immunocytochemical section of C6 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-NUP133 Antibody (AAA127290) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
NUP133, Polyclonal Antibody (Cat# AAA127290)
Full Name
Anti-NUP133 Antibody Picoband
Gene Names
NUP133; hNUP133
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Fanca/FAA antibody (AAA126330).Overlay histogram showing ANA-1 cells stained with AAA126330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fanca/FAA Antibody (AAA126330, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Fanca/FAA, Polyclonal Antibody (Cat# AAA126330)
Full Name
Anti-Fanca/FAA Antibody Picoband
Gene Names
Fanca; FACA; AW208693
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-ATR antibody (AAA125998).Overlay histogram showing U87 cells stained with AAA125998 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATR Antibody (AAA125998, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATR, Polyclonal Antibody (Cat# AAA125998)
Full Name
Anti-ATR Antibody Picoband
Gene Names
ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U2OS cells using anti-SERPINB13 antibody (AAA126555).Overlay histogram showing U2OS cells stained with AAA126555 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB13 Antibody (AAA126555, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SERPINB13, Polyclonal Antibody (Cat# AAA126555)
Full Name
Anti-SERPINB13 Antibody Picoband
Gene Names
SERPINB13; HUR7; PI13; headpin; HSHUR7SEQ; MGC126870
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (AAA126184).Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (AAA126184) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Histidine decarboxylase/HDC, Polyclonal Antibody (Cat# AAA126184)
Full Name
Anti-Histidine decarboxylase/HDC Antibody Picoband
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-NHP2 antibody (AAA127315).Overlay histogram showing SH-SY5Y cells stained with AAA127315 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NHP2 Antibody (AAA127315, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NHP2, Polyclonal Antibody (Cat# AAA127315)
Full Name
Anti-NHP2 Antibody Picoband
Gene Names
NHP2; DKCB2; NHP2P; NOLA2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of JK cells using anti-Smoothened/SMO antibody (AAA126028).Overlay histogram showing JK cells stained with AAA126028 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Smoothened/SMO Antibody (AAA126028, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Smoothened/SMO, Polyclonal Antibody (Cat# AAA126028)
Full Name
Anti-Smoothened/SMO Antibody Picoband
Gene Names
SMO; Gx; SMOH; FZD11
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of K562 cells using anti-DNA Ligase III/LIG3 antibody (AAA126249).Overlay histogram showing K562 cells stained with AAA126249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNA Ligase III/LIG3 Antibody (AAA126249, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DNA Ligase III/LIG3, Polyclonal Antibody (Cat# AAA126249)
Full Name
Anti-DNA Ligase III/LIG3 Antibody Picoband
Gene Names
LIG3; LIG2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Dexras1/RASD1 antibody (AAA126448).Overlay histogram showing HepG2 cells stained with AAA126448 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dexras1/RASD1 Antibody (AAA126448, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Dexras1/RASD1, Polyclonal Antibody (Cat# AAA126448)
Full Name
Anti-Dexras1/RASD1 Antibody Picoband
Gene Names
RASD1; AGS1; DEXRAS1; MGC:26290
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-nNOS (neuronal)/NOS1 antibody (AAA126104).Overlay histogram showing U87 cells stained with AAA126104 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-nNOS (neuronal)/NOS1 Antibody (AAA126104, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
nNOS (neuronal)/NOS1, Polyclonal Antibody (Cat# AAA126104)
Full Name
Anti-nNOS (neuronal)/NOS1 Antibody Picoband
Gene Names
NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of JK cells using anti-PSMB9 antibody (AAA127146).Overlay histogram showing JK cells stained with AAA127146 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB9 Antibody (AAA127146, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PSMB9, Polyclonal Antibody (Cat# AAA127146)
Full Name
Anti-PSMB9 Antibody Picoband
Gene Names
PSMB9; LMP2; PSMB6i; RING12; beta1i
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-IMP3/IGF2BP3 antibody (AAA126218).Overlay histogram showing U937 cells stained with AAA126218 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMP3/IGF2BP3 Antibody (AAA126218, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IMP3/IGF2BP3, Polyclonal Antibody (Cat# AAA126218)
Full Name
Anti-IMP3/IGF2BP3 Antibody Picoband
Gene Names
IGF2BP3; CT98; IMP3; KOC1; IMP-3; VICKZ3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells stained with TORC1 Monoclonal Antibody (red). followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control. normal mouse IgG.)
TORC1, Monoclonal Antibody (Cat# AAA320287)
Full Name
TORC1 Monoclonal Antibody
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human, Mouse, Rat, Pig
Applications
Immunocytochemistry, Immunofluorescence, Flow Cytometry, Western Blot
Purity
Affinity purification
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-Sox11 antibody (AAA126238).Overlay histogram showing ANA-1 cells stained with AAA126238 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sox11 Antibody (AAA126238, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sox11, Polyclonal Antibody (Cat# AAA126238)
Full Name
Anti-Sox11 Antibody Picoband
Gene Names
Sox11; end1; AI836553; 1110038H03Rik; 6230403H02Rik
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-RPS21 antibody (AAA127627).Overlay histogram showing HepG2 cells stained with AAA127627 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS21 Antibody (AAA127627, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS21, Polyclonal Antibody (Cat# AAA127627)
Full Name
Anti-RPS21 Antibody Picoband
Gene Names
RPS21; S21
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U251 cells using anti-Alpha Taxilin/TXLNA antibody (AAA126435).Overlay histogram showing U251 cells stained with AAA126435 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Taxilin/TXLNA Antibody (AAA126435, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alpha Taxilin/TXLNA, Polyclonal Antibody (Cat# AAA126435)
Full Name
Anti-Alpha Taxilin/TXLNA Antibody Picoband
Gene Names
TXLNA; IL14; TXLN; RP4-622L5.4
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Hela cells using anti-ACSL5 antibody (AAA127276).Overlay histogram showing Hela cells stained with AAA127276 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL5 Antibody (AAA127276, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ACSL5, Polyclonal Antibody (Cat# AAA127276)
Full Name
Anti-ACSL5 Antibody Picoband
Gene Names
ACSL5; ACS2; ACS5; FACL5
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of Jurkat cells using anti-DMC1 antibody (AAA126272).Overlay histogram showing Jurkat cells stained with AAA126272 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DMC1 Antibody (AAA126272, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DMC1, Polyclonal Antibody (Cat# AAA126272)
Full Name
Anti-DMC1 Antibody Picoband
Gene Names
DMC1; DMC1H; LIM15; dJ199H16.1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (AAA126363).CCXCR1/XCR1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CCXCR1/XCR1 Antibody (AAA126363) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
CCXCR1/XCR1, Polyclonal Antibody (Cat# AAA126363)
Full Name
Anti-CCXCR1/XCR1 Antibody Picoband
Gene Names
XCR1; GPR5; CCXCR1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of THP-1 cells using anti-ABP1/AOC1 antibody (AAA126517).Overlay histogram showing THP-1 cells stained with AAA126517 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABP1/AOC1 Antibody (AAA126517, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ABP1/AOC1, Polyclonal Antibody (Cat# AAA126517)
Full Name
Anti-ABP1/AOC1 Antibody Picoband
Gene Names
AOC1; ABP; DAO; KAO; ABP1; DAO1
Reactivity
Human, Monkey
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of THP-1 cells using anti-P2X7/P2RX7 antibody (AAA126125).Overlay histogram showing THP-1 cells stained with AAA126125 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P2X7/P2RX7 Antibody (AAA126125, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
P2X7/P2RX7, Polyclonal Antibody (Cat# AAA126125)
Full Name
Anti-P2X7/P2RX7 Antibody Picoband
Gene Names
P2RX7; P2X7
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of JK cells using anti-CD9 antibody (AAA126124).Overlay histogram showing JK cells stained with AAA126124 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (AAA126124, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD9, Polyclonal Antibody (Cat# AAA126124)
Full Name
Anti-CD9 Antibody Picoband
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
Standard Curve (Sample)
CNTF, ELISA Kit (Cat# AAA105901)
Full Name
Rat CNTF ELISA Kit
Reactivity
Rat
Applications
Sandwich ELISA
Pricing
Standard Curve (Sample)
IL-13, CLIA Kit (Cat# AAA318569)
Full Name
IL-13 (Murine) LumiAb ELISA Kit
Gene Names
Il13; Il-13
Reactivity
Mouse
Pricing
WB (Western Blot)
(Western blot analysis of extracts of mouse liver, using SCTR antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
SCTR, Polyclonal Antibody (Cat# AAA37101)
Full Name
SCTR Polyclonal Antibody
Gene Names
SCTR; SR
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.)
HAT-tag, Monoclonal Antibody (Cat# AAA40875)
Full Name
HAT-tag Antibody [4A6F3]
Applications
Immunoprecipitation, Western Blot
Purity
Protein A chromatography purified.
Pricing
Standard Curve (Sample)
BMP-5, ELISA Kit (Cat# AAA124762)
Full Name
Monkey primate BMP-5 PicoKine ELISA Kit
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Pricing
Standard Curve (Sample)
Endothelin, ELISA Kit (Cat# AAA124819)
Full Name
Chinese Hamster Endothelin PicoKine ELISA Kit
Gene Names
EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
Reactivity
Hamster
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Pricing
WB (Western Blot)
(Western blot analysis of (1) 20 ng of DDDDK-tagged recombinant GGP1 protein and (2) 20 ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.)
DDDDK-tag, Monoclonal Antibody (Cat# AAA40874)
Full Name
DDDDK-tag Antibody [1D1B12]
Applications
Immunoprecipitation, Western Blot
Purity
Protein G chromatography purified.
Pricing
SDS-PAGE
(SDS-PAGE)
Loricrin (LOR), Recombinant Protein (Cat# AAA152224)
Full Name
Recombinant Loricrin (LOR)
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(The OmniKine Rat EGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat EGF proteins within the range of 8-1000 pg/ml.)