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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1000-1050 of 2875 product results
Bioactivity
(Ribonuclease P (RNASEP) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme – a ribonucleic acid that acts as a catalyst in the same way that a protein based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on tRNA molecules. Besides, Methyl CpG Binding Protein 2 (MECP2) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RNASEP and recombinant human MECP2. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MECP2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of RNASEP and MECP2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of RNASEP with MECP2.)
Ribonuclease P, Active Protein (Cat# AAA150082)
Full Name
Active Ribonuclease P (RNASEP)
Gene Names
RPP40; RNASEP1; bA428J1.3
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
PTH, Active Protein (Cat# AAA214250)
Full Name
Recombinant Human PTH
Gene Names
PTH; PTH1
Purity
>95% as determined by SDS-PAGE
Pricing
Purity Data
(Sample Purity Data.)
SDS-PAGE
(The purity of KRAS was determined to be >90% by densitometry. Approx. MW 23kDa.)
SDS-PAGE
COVID 19 Spike S1 Protein Coronavirus, Active Protein (Cat# AAA176996)
Full Name
Recombinant 2019-nCoV S1 Protein (mFc Tag)
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
Glutathione S-Transferase Alpha 4, Active Protein (Cat# AAA39148)
Full Name
Recombinant Human Glutathione S-Transferase Alpha 4, Active
Gene Names
GSTA4; GTA4; GSTA4-4
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Gilthead Seabream Growth Hormone, Active Protein (Cat# AAA38553)
Full Name
Recombinant Gilthead Seabream Growth Hormone
Purity
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing
GRO/KC, Active Protein (Cat# AAA38092)
Full Name
Recombinant Mouse GRO/KC (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Tyrosine Phosphatase Non Receptor Type-6, Active Protein (Cat# AAA38392)
Full Name
Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-6
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Bioactivity
(Mouse LIFR alpha inhibits human LIF (Cat# ATGP3533) induced cell proliferation in the TF-1 human erythroleukemic cells. The ED50 range is )
LIFR alpha, Active Protein (Cat# AAA48367)
Full Name
Recombinant Mouse LIFR alpha Protein
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Application Data
VEGF189, Active Protein (Cat# AAA79232)
Full Name
Human VEGF189
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
IL-2, Active Protein (Cat# AAA79184)
Full Name
Human IL-2
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
FGF-4, Active Protein (Cat# AAA79203)
Full Name
Mouse FGF-4
Gene Names
Fgf4; KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; Hstf-1
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native rabbit hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)
Hemoglobin (HB), Active Protein (Cat# AAA161850)
Full Name
Active Hemoglobin (HB)
Gene Names
HBA; HBA1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Immobilized Mouse Ephrin A2/EFNA2 His at 2 ug/ml (100 ul/well) can bind Mouse EphA4 hFc, the EC50 of Mouse EphA4 hFc is 60-200 ng/mL.)
Ephrin A2/EFNA2, Active Protein (Cat# AAA258172)
Full Name
Ephrin A2/EFNA2 Protein, Mouse, Recombinant (His Tag)
Purity
>94% as determined by SDS-PAGE
Pricing
Creatine Kinase BB (CK-BB) Recombinant, Active Protein (Cat# AAA58337)
Full Name
CK-BB, Recombinant
Gene Names
CKB; B-CK; CKBB
Applications
ELISA
Purity
>95% pure (SDS-PAGE). Purified under non-denaturing conditions.
Pricing
Bioactivity
(Canine IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
IL-5, Active Protein (Cat# AAA48383)
Full Name
Recombinant canine IL-5 protein
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Application Data
Vascular Endothelial Growth Factor A (VEGFA), Active Protein (Cat# AAA146617)
Full Name
Active Vascular Endothelial Growth Factor A (VEGFA)
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF111; VEGF164
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Bioactivity
(Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Decorin (DCN) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FN and recombinant mouse DCN. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to DCN-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FN and DCN was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of FN with DCN.)
Fibronectin, Active Protein (Cat# AAA150050)
Full Name
Active Fibronectin (FN)
Gene Names
Fn1; Fn; Fn-1; E330027I09
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
Interleukin-2/IL-2) Biotinylated, Active Protein (Cat# AAA177957)
Full Name
Recombinant Human Interleukin-2/IL-2 (C-6His-Avi) Biotinylated
Gene Names
IL2; IL-2; TCGF; lymphokine
Purity
>90% as determined by reducing SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a.)
DKK1, Active Protein (Cat# AAA257837)
Full Name
DKK1 Protein, Human, Recombinant (His Tag)
Gene Names
DKK1; SK; DKK-1
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measure by its ability to bind with human PCSK9 in a functional ELISA. Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 ug/ml.)
LDLR/LDL Receptor, Active Protein (Cat# AAA257851)
Full Name
LDLR/LDL Receptor Protein, Human, Recombinant (His Tag)
Gene Names
LDLR; FH; FHC; LDLCQ2
Purity
>85% as determined by SDS-PAGE
Pricing
Carboxypeptidase-B, Active Protein (Cat# AAA38666)
Full Name
Recombinant Rat Carboxypeptidase-B
Gene Names
Cpb1; Cpb
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing
Matrix Metalloproteinase-2, Active Protein (Cat# AAA39014)
Full Name
Recombinant Human Matrix Metalloproteinase-2, HEK
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
Insulin Like Growth Factor-1, Active Protein (Cat# AAA38121)
Full Name
Recombinant Mouse Insulin Like Growth Factor-1
Gene Names
Igf1; Igf-1; Igf-I; C730016P09Rik
Purity
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Leptin, Active Protein (Cat# AAA38126)
Full Name
Recombinant Ovine Leptin
Gene Names
LEP; ob
Purity
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Interleukin-17A, Active Protein (Cat# AAA38132)
Full Name
Recombinant Human Interleukin-17A
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Neurotrophin-3, Active Protein (Cat# AAA38136)
Full Name
Recombinant Human Neurotrophin-3
Gene Names
NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Macrophage Inflammatory protein-3 alpha, Active Protein (Cat# AAA38581)
Full Name
Recombinant Mouse Macrophage Inflammatory protein-3 alpha (CCL20)
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
IL-12 receptor beta 1, Active Protein (Cat# AAA76114)
Full Name
Human IL-12 receptor beta 1 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Mouse CD200 is coated at 1 ug/ml (100 ul/well) can bind CD200 R1 (CAT# ATGP3457) in a Functional ELISA assay.)
CD200, Active Protein (Cat# AAA48373)
Full Name
Recombinant mouse CD200 protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
TGF-beta RI/ALK-5, Active Protein (Cat# AAA48990)
Full Name
Recombinant human TGF-beta RI/ALK-5 protein
Gene Names
TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Application Data
(Interleukin 1 beta (IL-1) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1 can induced IL-8 production in A549 cells. To test the bioactivity of IL-1, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations (0.1ng/mL-1000ng/mL) of IL-1 for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1 have shown in Figure1.Figure. IL-8 levels in the cell supernatant of A549 induced by IL-1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150092)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
PDGFA, Active Protein (Cat# AAA214228)
Full Name
Recombinant Human PDGFA
Gene Names
PDGFA; PDGF1; PDGF-A
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
GSTA1, Active Protein (Cat# AAA48584)
Full Name
GSTA1, 1-222aa, Human, E Coli (Bioactivity Validated)
Gene Names
GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
Applications
Enzyme Activity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
WB (Western Blot)
COVID 19 Spike S Protein (RBD) Coronavirus, Active Protein (Cat# AAA176991)
Full Name
Recombinant 2019-nCoV Spike S Protein (RBD, mFc Tag)
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
IFN GAMMA, Active Protein (Cat# AAA50533)
Full Name
RECOMBINANT HUMAN INTERFERON GAMMA
Gene Names
IFNG; IFG; IFI
Applications
Western Blot, Functional Assay
Purity
>98% by SDS PAGE and HPLC
Pricing
Glucose-6-Phosphate Dehydrogenase, Active Protein (Cat# AAA38735)
Full Name
Recombinant E Coli Glucose-6-Phosphate Dehydrogenase
Gene Names
G6PD; G6PD1
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
Persephin, Active Protein (Cat# AAA39062)
Full Name
Recombinant Human Persephin
Gene Names
PSPN; PSP
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
Interleukin-15, Active Protein (Cat# AAA39137)
Full Name
Recombinant Human Interleukin-15, HEK
Gene Names
IL15; IL-15
Purity
Greater than 95.0% as determined by SDS-PAGE.
Granulocyte-Colony Stimulating Factor, Active Protein (Cat# AAA38194)
Full Name
Recombinant Mouse Granulocyte-Colony Stimulating Factor
Gene Names
Csf3; Csfg; G-CSF; MGI-IG
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
MERS-CoV Spike, Active Protein (Cat# AAA48982)
Full Name
Recombinant MERS-CoV Spike protein
Applications
Bioactivity, SDS-PAGE
Purity
> 85% by SDS-PAGE
Pricing
Application Data
IL33, Active Protein (Cat# AAA214237)
Full Name
Recombinant Human IL33
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His is 200-800 ng/mL.)
COVID 19 Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Coronavirus, Active Protein (Cat# AAA258080)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Recombinant Protein
Purity
>85% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2ug/mL (100uL/well) can bind S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc, the EC50 of S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc is 1.5-8.0 ng/mL.)
COVID 19 Spike RBD (N501Y) Protein (Fc Tag) Coronavirus, Active Protein (Cat# AAA258093)
Full Name
SARS-CoV-2 (2019-nCoV) Spike RBD (N501Y) Protein (Fc Tag)
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 2.5-10 ug/mL.)
BMPR1A/ALK-3, Active Protein (Cat# AAA257883)
Full Name
BMPR1A/ALK-3 Protein, Human, Recombinant (His Tag)
Gene Names
BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
Purity
>85% as determined by SDS-PAGE
Pricing
Purity Info
PDE7A, Active Protein (Cat# AAA72929)
Full Name
PDE7A, Active recombinant protein
Gene Names
PDE7A; HCP1; PDE7
Purity
>90%
Pricing
ACE2, Active Protein (Cat# AAA78494)
Full Name
Human ACE2 Protein (Fc Tag)
Purity
>=90% (SDS-PAGE)
Pricing
Application Data
VEGF-C, Active Protein (Cat# AAA79180)
Full Name
Rat VEGF-C
Reactivity
Rat
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Keratinocyte Growth Factor-2, Active Protein (Cat# AAA38945)
Full Name
Recombinant Mouse Keratinocyte Growth Factor-2
Gene Names
Fgf10; AEY17; Fgf-10; BB213776; Gsfaey17
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.