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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1150-1200 of 2567 product results
DLL-4, soluble, Active Protein (Cat# AAA79284)
Full Name
Human DLL-4, soluble
Gene Names
DLL4; hdelta2
Purity
> 95%, by SDS-PAGE and HPLC analysis
Pricing
Application Data
EGF, Active Protein (Cat# AAA79304)
Full Name
Human EGF
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153016)
Full Name
Active Galectin 4 (GAL4)
Gene Names
LGALS4; GAL4; L36LBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Pregnancy-associated plasma protein A(PAPPA), also known as pappalysin-1, is a secreted protease whose main substrate is insulin-like growth factor binding proteins. PAPPA's proteolytic function is activated upon collagen binding. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). Besides, Plasminogen (Plg) has been identified as an interactor of PAPPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PAPPA and recombinant human Plg. Briefly, biotinylated PAPPA were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to Plg-coated microtiter wells and incubated for 1 h at 37 degree C. Wells were washed 3 times with PBST and incubated for 0.5 h at 37 degree C with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of PAPPA and Plg was shown in Figure 1, and the ED50 for this effect is 0.04936 ug/ml.)
Pregnancy Associated Plasma Protein A (PAPPA), Active Protein (Cat# AAA152234)
Full Name
Active Pregnancy Associated Plasma Protein A (PAPPA)
Gene Names
PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates. Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B). The full-length (amino acid residues 1-707) of mouse C1r was expressed which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 50 mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50 uL of various concentrations of C1r (diluted by Assay Buffer) to 50 ul substrate mixture of 0.2mM Z-Lys-SBzl•HCl and 0.2 mM DTNB. The final well serves as a negative control with no C1r, replaced with 50 ul assay buffer. Then read in kinetic mode for 5 minutes at an absorbance of 405 nm. The specific activity of recombinant mouse C1r is > 500 pmol/min/ug.)
Complement Component 1, R Subcomponent (C1r), Active Protein (Cat# AAA161831)
Full Name
Active Complement Component 1, R Subcomponent (C1r)
Gene Names
C1ra; C1r; mC1rA; AI132558
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 uL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 degree C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/ug.)
Hyaluronidase (HAase), Active Protein (Cat# AAA161835)
Full Name
Active Hyaluronidase (HAase)
Gene Names
HYAL1; NAT6; LUCA1; HYAL-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 9 (C9), a component of the Membrane attack Complex (MAC) plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. During MAC assembly, multiple copies of C9 are sequentially recruited to membrane associated C5b8 to form a pore. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C9 and recombinant human C5. Briefly, biotin-linked C9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C5-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human C9 and recombinant human C5 was shown in Figure 1, the EC50 for this effect is 0.13 ug/mL.)
Complement Component 9 (C9), Active Protein (Cat# AAA161862)
Full Name
Active Complement Component 9 (C9)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation. It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The activity of recombinant rat TFPI was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 20 ul different concentrations of recombinant rat TFPI (MW: 61.9 KD) was incubated with 20 ul diluted trypsin at 37 degree C for 15 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 20 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TFPI significantly decreased trypsin activity. The inhibition IC50 was )
Tissue Factor Pathway Inhibitor (TFPI), Active Protein (Cat# AAA161732)
Full Name
Active Tissue Factor Pathway Inhibitor (TFPI)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
prolactin soluble receptor, Active Protein (Cat# AAA60571)
Full Name
Bovine prolactin soluble receptor
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) Gel filtration at pH 8 under non denaturative conditions.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) Gel filtration at pH 8 under non denaturative conditions.
Interleukin-6, Active Protein (Cat# AAA38185)
Full Name
Recombinant Rat Interleukin-6
Gene Names
Il6; ILg6; Ifnb2
Purity
Greater than 95.0% as determined by:
Analysis by RP-HPLC
Analysis by SDS-PAGE
Analysis by RP-HPLC
Analysis by SDS-PAGE
Pricing
Complement C7, Active Protein (Cat# AAA75234)
Full Name
Complement C7 protein
Purity
> 98% pure
Pricing
Complement Factor B, Active Protein (Cat# AAA75246)
Full Name
Complement Factor B
Gene Names
CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
Purity
> 95% by SDS-PAGE
Pricing
Cathepsin D, Active Protein (Cat# AAA75402)
Full Name
Cathepsin D protein
Gene Names
CTSD; CPSD; CLN10; HEL-S-130P
Purity
> 98% pure
Pricing
Insulin, Active Protein (Cat# AAA75422)
Full Name
Insulin protein
Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
Purity
> 98% (by SDS-PAGE and RP-HPLC)
Pricing
IL6, Active Protein (Cat# AAA75428)
Full Name
IL6 protein
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Purity
> 98% pure
Pricing
Trefoil Factor-2, Active Protein (Cat# AAA38603)
Full Name
Recombinant Human Trefoil Factor-2
Gene Names
TFF2; SP; SML1
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Factor V, Active Protein (Cat# AAA71836)
Full Name
Human Factor V
Gene Names
F5; FVL; PCCF; THPH2; RPRGL1
Purity
Purity: >95% by SDS-PAGE analysis
Purification: Immunoaffinity chromatography
Purification: Immunoaffinity chromatography
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 1-25 ng/ml.)
EphB4, Active Protein (Cat# AAA257852)
Full Name
EphB4 Protein, Human, Recombinant (His Tag)
Gene Names
EPHB4; HTK; MYK1; TYRO11
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse PD-L2-his at 10 ug/mL (100 ul/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1.63 ug/mL.)
PD-L2, Active Protein (Cat# AAA258205)
Full Name
PD-L2 Protein, Mouse, Recombinant (His Tag)
Gene Names
Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized canine NTRK-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 0.07-0.17 ug/ml.)
TrkB, Active Protein (Cat# AAA258251)
Full Name
TrkB Protein, Canine, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 ug/ml (100 ul/well) can bind human ICOS with a linear range of 40-1000 ng/ml.)
ICOS ligand, Active Protein (Cat# AAA258141)
Full Name
ICOS ligand Protein, Mouse, Recombinant (His Tag)
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EFNA1-His at 10 ug/ml (100 ul /well) can bind human EPHA1-Fc, The EC50 of human EPHA1-Fc is 10-30 ng/ml.)
EphA1, Active Protein (Cat# AAA258037)
Full Name
EphA1 Protein, Human, Recombinant (hFc Tag)
Gene Names
EPHA1; EPH; EPHT; EPHT1
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1),the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL.)
SARS-CoV Spike/S1, Active Protein (Cat# AAA258071)
Full Name
SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)
Reactivity
SARS
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1) is 400-1200 ng/mL.)
COVID 19 Spike S1+S2 ECD-His Coronavirus, Active Protein (Cat# AAA258075)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Pricing
SDS-PAGE
SPARC, Active Protein (Cat# AAA258167)
Full Name
SPARC Protein, Mouse, Recombinant (His Tag)
Gene Names
Sparc; ON; BM-40
Purity
>96% as determined by SDS-PAGE
Pricing
Bioactivity
Ephrin Type-A Receptor 3 (EPHA3), Active Protein (Cat# AAA243729)
Full Name
Recombinant Human Ephrin Type-A Receptor 3 (EPHA3), Partial (Active)
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Decorin (DCN) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FN and recombinant mouse DCN. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to DCN-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FN and DCN was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of FN with DCN.)
Fibronectin, Active Protein (Cat# AAA150050)
Full Name
Active Fibronectin (FN)
Gene Names
Fn1; Fn; Fn-1; E330027I09
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
(Figure 1.Gelatin hydrolysis by recombinant human MMP13.)
Matrix Metalloproteinase 13, Active Protein (Cat# AAA150069)
Full Name
Active Matrix Metalloproteinase 13 (MMP13)
Gene Names
MMP13; CLG3; MDST; MANDP1; MMP-13
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure. The binding activity of TGF-1 with LTBP1.Transforming growth factor beta 1 or TGF-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Besides, Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) has been identified as an interactor of TGF-1, thus a binding ELISA assay was conducted to detect the interaction of recombinant pig TGF-1 and recombinant pig LTBP1. Briefly, TGF-1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to LTBP1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TGF-1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-1 and LTBP1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Transforming Growth Factor Beta 1, Active Protein (Cat# AAA150075)
Full Name
Active Transforming Growth Factor Beta 1 (TGFb1)
Gene Names
TGFB1; TGF-BETA-1
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TIMP4 and recombinant human MMP2. Briefly, TIMP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TIMP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TIMP4 and MMP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Tissue Inhibitors Of Metalloproteinase 4, Active Protein (Cat# AAA150077)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)
Gene Names
TIMP4; TIMP-4
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
(Figure. Inhibition of FGF basic-dependent HUVEC proliferation after stimulated with PF4.)
Platelet Factor 4, Active Protein (Cat# AAA150080)
Full Name
Active Platelet Factor 4 (PF4)
Gene Names
Pf4; Cxcl4; Scyb4
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
Vascular Endothelial Growth Factor A (VEGFA), Active Protein (Cat# AAA146617)
Full Name
Active Vascular Endothelial Growth Factor A (VEGFA)
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF111; VEGF164
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Bioactivity
(Figure. The binding activity of FGF19 with FGFR1.Fibroblast Growth Factor 19 (FGF19) is a member of the fibroblast growth factor (FGF) family. FGF19 has important roles as a hormone produced in the ileum in response to bile acid absorption, regulates new bile acid synthesis, acting through the FGFR4/Klotho- receptor complexes in the liver to inhibit CYP7A1. FGF19 also has metabolic effects, affecting glucose and lipid metabolism. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF19, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF19 and recombinant human FGFR1. Briefly, FGF19 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-FGF19 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF19 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Fibroblast Growth Factor 19, Active Protein (Cat# AAA150129)
Full Name
Active Fibroblast Growth Factor 19 (FGF19)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Figure. The binding activity of PTEN with TP53.Phosphatase and tensin homolog (PTEN) is a protein is widely expressed throughout the body. PTEN protein acts as a phosphatase to dephosphorylate phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5)P3 or PIP3). PTEN specifically catalyses the dephosphorylation of the 3’ phosphate of the inositol ring in PIP3, resulting in the biphosphate product PIP2 (PtdIns (4,5) P2). PTEN also has weak protein phosphatase activity, but this activity is also crucial for its role as a tumor suppressor. PTENs protein phosphatase activity may be involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of PTEN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PTEN and recombinant human TP53. Briefly, PTEN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to TP53-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PTEN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PTEN and TP53 was shown in Figure 1, and this effect was in a dose dependent manner.)
Phosphatase And Tensin Homolog, Active Protein (Cat# AAA150140)
Full Name
Active Phosphatase And Tensin Homolog (PTEN)
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Bioactivity
(Figure. The binding activity of PRKG2 with HSP90aA1.Protein Kinase, cGMP Dependent Type II (PRKG2) belong to cGMP-dependent protein kinase or Protein Kinase G (PKG) which is a serine/threonine-specific protein kinase that is activated by cGMP. Two PKG genes, coding for PKG type I (PKG-I) and type II (PKG-II), have been identified in mammals. The PKG-I and PKG-II are homodimers of two identical subunits (~75kDa and ~85kDa, respectively) and share common structural features. PKG phosphorylates a number of biologically important targets and is implicated in the regulation of smooth muscle relaxation, platelet function, sperm metabolism, cell division, and nucleic acid synthesis. Besides, Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) has been identified as an interactor of PRKG2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRKG2 and recombinant human HSP90aA1. Briefly, PRKG2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP90aA1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PRKG2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRKG2 and HSP90aA1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Protein Kinase, cGMP Dependent Type II, Active Protein (Cat# AAA150142)
Full Name
Active Protein Kinase, cGMP Dependent Type II (PRKG2)
Gene Names
PRKG2; PKG2; cGK2; cGKII; PRKGR2
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150562)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Gene Names
Ace2; 2010305L05Rik
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Growth Hormone (rHuGH), Active Protein (Cat# AAA47952)
Full Name
Recombinant Human Growth Hormone
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Purity
Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Purification: Chromatographic
Purification: Chromatographic
Pricing
Tissue Plasminogen Activator, Active Protein (Cat# AAA76078)
Full Name
Human Tissue Plasminogen Activator Recombinant
Purity
>98%, as determined by SDS-PAGE and HPLC
CD155, Active Protein (Cat# AAA76100)
Full Name
Human CD155 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Biotinylated COV2 S trimer(coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml)
COVID 19 Spike protein S Trimer Coronavirus, Active Protein (Cat# AAA76126)
Full Name
Biotinylated SARS COV2 Spike protein S Trimer Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
ELISA
(SARS COV2 RBD B.1.1.529/omicron ACE functional binding ELISA assay. COV2 RBD (coating at 0.1ug-well) binding with Human ACE2-Fc .The linear range was found to be 100 ng/ml)
COVID 19 B.1.1.529 (Omicron) Spike RBD Coronavirus, Active Protein (Cat# AAA76129)
Full Name
COV2 Spike protein RBD Recombinant B.1.1.529 (Omicron)-His
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(Human IL-1ra/IL-1F3 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ?10 ng/ml.)
IL-1RA/IL-1F3, Active Protein (Cat# AAA48355)
Full Name
Recombinant human IL-1RA/IL-1F3 protein
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS - PAGE
Pricing
Bioactivity
(Human Angiopoietin-2 is coated at 5 ug/ml (100 ul/well) can bind Human Tie-2. The ED50 range )
Tie-2, Active Protein (Cat# AAA48363)
Full Name
Recombinant human Tie-2 protein
Gene Names
TEK; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Bioactivity
(SARS-CoV Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)
COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48374)
Full Name
Recombinant SARS-CoV Spike RBD protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)
COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48376)
Full Name
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Application Data
(TGF-? is a negative regulator of NK cell generation CD11cdnR and wild-type mice were injected (i.p.)Four days later, mice were sacrificed and HSC-enriched bone marrow cells were cultured in the presence of IL-7, SCF, and Flt3L.)
Interleukin-7, Active Protein (Cat# AAA76391)
Full Name
Mouse Interleukin-7 Recombinant
Gene Names
Il7; Il-7; hlb368; A630026I06Rik
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Transforming Growth Factor-Beta 2, Active Protein (Cat# AAA76534)
Full Name
Transforming Growth Factor-Beta 2, Recombinant Protein
Gene Names
TGFB2; TGF-beta-2
Purity
Greater than 97.0% as determined by SDS-PAGE.
Pricing
IL-21, Active Protein (Cat# AAA48978)
Full Name
Recombinant Animal Free Human IL-21 Protein
Gene Names
IL21; Za11; IL-21; CVID11
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
TMIGD2/CD28H, Active Protein (Cat# AAA48995)
Full Name
Recombinant human TMIGD2/CD28H protein
Gene Names
TMIGD2; CD28H; IGPR1; IGPR-1
Applications
Bioactivity, SDS-PAGE
Purity
95% by SDS-PAGE
Pricing
SDS-PAGE
SHP-1, Active Protein (Cat# AAA48446)
Full Name
SHP-1,243-541aa, Human, E Coli (Bioactivity Validated)
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.