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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1400-1450 of 2567 product results
Bioactivity
(CD200 Subunit is coated at 10 ug/ml (100 ul/well) can bind Human CD200R1 (CAT# ATGP3809) in a Functional ELISA assay.)
CD200, Active Protein (Cat# AAA48391)
Full Name
Recombinant human CD200 protein
Gene Names
CD200; MRC; MOX1; MOX2; OX-2
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(Human TL1A/TNFSF15 (CAT# ATGP1049) is coated at 10 ug/ml (100 ul/well) can bind Human DR3/TNFRSF25. The ED50 range )
DR3/TNFRSF25, Active Protein (Cat# AAA48394)
Full Name
Recombinant human DR3/TNFRSF25 protein
Gene Names
TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(Human EPHB1 is coated at 2 ug/ml (100 ul/well) can bind Human EFNB1 (CAT# ATGP3800) in a Functional ELISA assay.)
EphB1, Active Protein (Cat# AAA48404)
Full Name
Recombinant human EphB1 protein
Gene Names
EPHB1; ELK; NET; Hek6; EPHT2
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range )
Osteopontin/OPN, Active Protein (Cat# AAA48410)
Full Name
Recombinant mouse Osteopontin/OPN protein
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Bioactivity
(Human IL-9 stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )
IL-9, Active Protein (Cat# AAA48322)
Full Name
Recombinant IL-9
Gene Names
IL9; P40; HP40; IL-9
Applications
Bioactivity, SDS-PAGE
Purity
> 85% by SDS - PAGE
Pricing
SDS-PAGE
Interleukin-3, Active Protein (Cat# AAA48476)
Full Name
Interleukin-3, 20-152aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
SOD1, Active Protein (Cat# AAA48526)
Full Name
SOD1, 1-154aa, Human, E Coli (Bioactivity Validated)
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
TGF-beta RII, Active Protein (Cat# AAA48997)
Full Name
Recombinant human TGF-beta RII protein
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Interferon alpha-2b, Active Protein (Cat# AAA62203)
Full Name
Human Interferon alpha-2b (INF alpha-2b), yeast
Purity
> 97% (by SDS-PAGE and HPLC)
Pricing
IGF-1, Active Protein (Cat# AAA79164)
Full Name
Human IGF-1
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
VE-Statin/EGFL-7, Active Protein (Cat# AAA79165)
Full Name
Human VE-Statin/EGFL7
Gene Names
EGFL7; NEU1; ZNEU1; VE-STATIN; RP11-251M1.2
Reactivity
Human
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
RELM alpha, Active Protein (Cat# AAA79173)
Full Name
Mouse RELM alpha
Gene Names
Retnla; HIMF; Xcp2; Fizz1; RELMa; Fizz-1; RELMalpha; RELM-alpha; 1810019L16Rik
Reactivity
Mouse
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
Application Data
(Immobilized Human BAFF/BLyS hFc at 2 ug/mL (100 uL/well) can bind Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated, the EC50 of Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated is 40-200 ng/mL.)
BAFFR/TNFRSF13C, Active Protein (Cat# AAA258045)
Full Name
BAFFR/TNFRSF13C Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated
Gene Names
TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD47-His at 2 ug/ml (100 ul/well) can bind human SIRPa(V2)-mFc, the EC50 of human SIRPa(V2)-mFc is 2-14 ng/mL.)
SIRP alpha, Active Protein (Cat# AAA258063)
Full Name
SIRP alpha Protein, Human, Recombinant (ECD, SIRP alphaV2, mFc Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 ug/ml (100 ul/well) can bind biotinylated human DPP4. The EC50 of of biotinylated DPP4 is 0.6-1.39 ug/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 ug/ml (100 ul/well) can bind biotinylated Fc-DPP4. The EC50 of biotinylated Fc-DPP4 is 0.02-0.05 ug/ml.)
MERS-CoV Spike/S1, Active Protein (Cat# AAA258067)
Full Name
MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 (D80A, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) (ECD, His Tag), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 (D80A, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) (ECD, His Tag) is 100-700ng/mL.)
COVID 19 Spike S1+S2 (D80A, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) Coronavirus, Active Protein (Cat# AAA258083)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1+S2 (D80A, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag)
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. 1. Immobilized mouse ErbB3-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125 ug/ml. 2. Immobilized mouse ErbB3-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 6.25-100 ng/ml.)
HER3/ERBB3, Active Protein (Cat# AAA258220)
Full Name
HER3/ERBB3 Protein, Mouse, Recombinant (His Tag)
Gene Names
Erbb3; Her3; C76256; Erbb-3; Erbb3r
Purity
>93% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 ug/ml (100 ul/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/ml.)
CD200R, Active Protein (Cat# AAA258144)
Full Name
CD200R Protein, Mouse, Recombinant (His & hFc Tag)
Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2 ug/ml (100 ul/well) can bind mouse CD155 with a linear ranger of 6.4-160 ng/ml.)
CD226, Active Protein (Cat# AAA258146)
Full Name
CD226 Protein, Mouse, Recombinant (His Tag)
Gene Names
Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His at 2 ug/ml (100 ul/well) can bind Mouse CD40 Ligand hFc, the EC50 of Mouse CD40 Ligand hFc is 60-300 ng/mL.)
CD40 Ligand, Active Protein (Cat# AAA258155)
Full Name
CD40 Ligand Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Purity
>80% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Mouse Ephrin B2hFc at 2 ug/ml (100 ul/well) can bind Mouse EphB4 His, the EC50 of Mouse EphB4 His is 3.5-20 ng/mL.)
EphB4, Active Protein (Cat# AAA258174)
Full Name
EphB4 Protein, Mouse, Recombinant (His Tag)
Gene Names
Ephb4; Htk; MDK2; Myk1; Tyro11; AI042935; b2b2412Clo
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human NCR3 at 10 ug/mL (100 uL/well) can bind human B7-H6, the EC50 of human B7-H6 is 20-100 ng/mL.)
NKp30/NCR3, Active Protein (Cat# AAA257889)
Full Name
NKp30/NCR3 Protein, Human, Recombinant (His Tag)
Gene Names
NCR3; 1C7; MALS; CD337; LY117; NKp30
Purity
>97% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Sample: Active recombinant AST, Human)
Aspartate Aminotransferase (AST), Active Protein (Cat# AAA152060)
Full Name
Active Aspartate Aminotransferase (AST)
Gene Names
GOT1; cCAT; GIG18; cAspAT; ASTQTL1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
SDS-PAGE
(SDS-PAGE)
Acid Sphingomyelinase (ASM), Active Protein (Cat# AAA152129)
Full Name
Active Acid Sphingomyelinase (ASM)
Gene Names
SMPD1; ASM; NPD; ASMASE
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Figure. The chemotactic effect of recombinant human MIP3b on Jurkat cells.)
Macrophage Inflammatory Protein 3 Beta, Active Protein (Cat# AAA150068)
Full Name
Active Macrophage Inflammatory Protein 3 Beta (MIP3b)
Gene Names
CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Figure. The binding activity of PK2 with PKR1)
Prokineticin 2, Active Protein (Cat# AAA150071)
Full Name
Active Prokineticin 2 (PK2)
Gene Names
PROK2; BV8; HH4; PK2; KAL4; MIT1
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Ribonuclease P (RNASEP) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme – a ribonucleic acid that acts as a catalyst in the same way that a protein based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on tRNA molecules. Besides, Methyl CpG Binding Protein 2 (MECP2) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RNASEP and recombinant human MECP2. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MECP2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of RNASEP and MECP2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of RNASEP with MECP2.)
Ribonuclease P, Active Protein (Cat# AAA150082)
Full Name
Active Ribonuclease P (RNASEP)
Gene Names
RPP40; RNASEP1; bA428J1.3
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
(Figure. Cell proliferation of 3T3 cells after stimulated with EGF.)
Epidermal Growth Factor, Active Protein (Cat# AAA150091)
Full Name
Active Epidermal Growth Factor (EGF)
Gene Names
Egf; AI790464
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure. The binding activity of UGCG with LMP2.)
UDP Glucose Ceramide Glucosyltransferase, Active Protein (Cat# AAA150115)
Full Name
Active UDP Glucose Ceramide Glucosyltransferase (UGCG)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Figure. Western Blot)
Platelet Derived Growth Factor D, Active Protein (Cat# AAA150130)
Full Name
Active Platelet Derived Growth Factor D (PDGFD)
Gene Names
PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
(Figure. The binding activity of GDNF with GFRa2.Glial cell-derived neurotrophic factor (GDNF) is a small protein that potently promotes the survival of many types of neurons. This protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Besides, Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 2 (GFRa2) has been identified as an interactor of GDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDNF and recombinant human GFRa2. Briefly, GDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GFRa2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-GDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GDNF and GFRa2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Regenerating Islet Derived Protein 3 Alpha, Active Protein (Cat# AAA150135)
Full Name
Active Regenerating Islet Derived Protein 3 Alpha (REG3a)
Gene Names
REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150561)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Gene Names
ACE2; ACEH
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45a) is a member of the GADD45 family. It may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Besides, Proliferating Cell Nuclear Antigen (PCNA) has been identified as an interactor of GADD45a, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GADD45a and recombinant rat PCNA. Briefly, GADD45a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to PCNA-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-GADD45a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GADD45a and PCNA was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of GADD45a with PCNA.)
Growth Arrest And DNA Damage Inducible Protein Alpha, Active Protein (Cat# AAA150146)
Full Name
Active Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)
Gene Names
Gadd45a; Ddit1; Gadd45
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
NKG2-D Type II Integral Membrane (KLRK1), Active Protein (Cat# AAA243731)
Full Name
Recombinant Human NKG2-D Type II Integral Membrane Protein (KLRK1), Partial (Active)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), Active Protein (Cat# AAA243733)
Full Name
Recombinant Human Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), Partial (Active)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observ)
Annexin V (ANXA5), Active Protein (Cat# AAA153011)
Full Name
Active Annexin V (ANXA5)
Gene Names
Anxa5; Anx5; R74653
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Apolipoprotein A2 (APOA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of APOA2 are associated with disorders of ch)
Apolipoprotein A2 (APOA2), Active Protein (Cat# AAA153050)
Full Name
Active Apolipoprotein A2 (APOA2)
Gene Names
APOA2; apoAII; Apo-AII; ApoA-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Aspartate transaminase(AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or glutamic oxaloacetic transaminase, is a pyridoxal phosphate-dependent transaminase enzyme AST catalyzes the reversible transfer of an alpha-amino group between aspa)
Aspartate Aminotransferase (AST), Active Protein (Cat# AAA153078)
Full Name
Active Aspartate Aminotransferase (AST)
Gene Names
Got1; cCAT; Got-1; cAspAT; AI789014
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Lysophosphatidic acid phosphatase type 6 (ACP6), is a 44-47 kDa monomeric member of the histidine acid phosphatase family of proteins. ACP6 is highly expressed in kidney, heart, small intestine, muscle, liver, prostate, testis, ovary and exists as two isoforms as a result of alternative splicing events. It plays a role in regulating lipid metabolism that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Annexin V (ANXA5) binds to phospholipid bilayers, forming two-dimensional crystals that block the phospholipids from availability for coagulation enzyme reactions. A functional binding ELISA assay was conducted to detect the interaction of recombinant human ACP6 and recombinant mouse ANXA5. Briefly, ACP6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ANXA5-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ACP6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human ACP6 and recombinant mouse ANXA5 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.)
Acid Phosphatase 6, Lysophosphatidic (ACP6), Active Protein (Cat# AAA161908)
Full Name
Active Acid Phosphatase 6, Lysophosphatidic (ACP6)
Gene Names
ACP6; LPAP; ACPL1; PACPL1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)
Janus Kinase 1 (JAK1), Active Protein (Cat# AAA161890)
Full Name
Active Janus Kinase 1 (JAK1)
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Insulin-like growth factor 2 (IGF2) is one of three protein hormones that share structural similarity to insulin. It has growth-regulating, insulin-like and mitogenic activities. IGF2 exerts its effects by binding to the IGF-1 receptor and to the short isoform of the insulin receptor. IGF2 may also bind to the IGF2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. Besides, Insulin Like Growth Factor Binding Protein 2 (IGFBP2) has been identified as an interactor of IGF2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IGF2 and recombinant mouse IGFBP2. Briefly, IGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IGFBP2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse IGF2 and recombinant mouse IGFBP2 was shown in Figure 1, the EC50 for this effect is 0.009 ug/mL.)
Insulin Like Growth Factor 2 (IGF2), Active Protein (Cat# AAA161671)
Full Name
Active Insulin Like Growth Factor 2 (IGF2)
Gene Names
Igf2; Mpr; M6pr; Peg2; Igf-2; Igf-II; AL033362
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development. To test the effect of OSM on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human OSM. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with OSM for 72 hours observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human OSM for 72 hours. The result was shown in Figure 2. It was obvious that recombinant human OSM significantly increased cell viability of TF-1 cells, the EC50 was 0.08 ng/ml.)
Oncostatin M (OSM), Active Protein (Cat# AAA161690)
Full Name
Active Oncostatin M (OSM)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant bovine TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rbTIMP2 (MW: 51.75 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rhTIMP2 curve dilutions, 12.8 ul of activated rbMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant bovine TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161695)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Apolipoprotein A1 (APOA1) is the major protein component of HDL particles in plasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. Besides, Plasminogen Activator, Urokinase (uPA) has been identified as an interactor of APOA1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOA1 and recombinant human uPA. Briefly, biotin-linked APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to uPA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human uPA and recombinant human APOA1 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.)
Apolipoprotein A1 (APOA1), Active Protein (Cat# AAA161738)
Full Name
Active Apolipoprotein A1 (APOA1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Ephrin Type-A Receptor 3 (EPHA3), Active Protein (Cat# AAA243729)
Full Name
Recombinant Human Ephrin Type-A Receptor 3 (EPHA3), Partial (Active)
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 1-25 ng/ml.)
EphB4, Active Protein (Cat# AAA257852)
Full Name
EphB4 Protein, Human, Recombinant (His Tag)
Gene Names
EPHB4; HTK; MYK1; TYRO11
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EFNA1-His at 10 ug/ml (100 ul /well) can bind human EPHA1-Fc, The EC50 of human EPHA1-Fc is 10-30 ng/ml.)
EphA1, Active Protein (Cat# AAA258037)
Full Name
EphA1 Protein, Human, Recombinant (hFc Tag)
Gene Names
EPHA1; EPH; EPHT; EPHT1
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1),the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL.)
SARS-CoV Spike/S1, Active Protein (Cat# AAA258071)
Full Name
SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)
Reactivity
SARS
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1) is 400-1200 ng/mL.)
COVID 19 Spike S1+S2 ECD-His Coronavirus, Active Protein (Cat# AAA258075)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse PD-L2-his at 10 ug/mL (100 ul/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1.63 ug/mL.)
PD-L2, Active Protein (Cat# AAA258205)
Full Name
PD-L2 Protein, Mouse, Recombinant (His Tag)
Gene Names
Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
Purity
>95% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.