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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2750-2800 of 2875 product results
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150563)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Alpha-1-Antitrypsin, Active Protein (Cat# AAA11467)
Full Name
Alpha-1-Antitrypsin (A1AT), Human Serum
Purity
> 95% (SDS-PAGE)
Pricing
Bioactivity
(Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerizati)
Thymosin Beta 4 (TMSB4X), Active Protein (Cat# AAA153102)
Full Name
Active Thymosin Beta 4 (TMSB4X)
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Studies have shown that fibrinogen can enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse FGB and recombinant human ITGb3. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ITGb3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse FGB and recombinant human ITGb3 was shown in Figure 1, the EC50 for this effect is 0.016 ug/mL.)
Fibrinogen Beta Chain (FGB), Active Protein (Cat# AAA161834)
Full Name
Active Fibrinogen Beta Chain (FGB)
Gene Names
Fgb; 2510049G14Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
alpha-Amylase, Active Protein (Cat# AAA11469)
Full Name
a-Amylase, Human Pancreas
Gene Names
AMY2A; PA; AMY2
Pricing
HVEM-Fc, Active Protein (Cat# AAA14394)
Full Name
HVEM-Fc protein
Purity
>= 98% by SDS-PAGE and HPLC
Pricing
Osteoprotegerin/Fc Chimera, Active Protein (Cat# AAA10811)
Full Name
Recombinant Human Osteoprotegerin/Fc Chimera
Gene Names
TNFRSF11B; OPG; TR1; OCIF
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His is 10-60 ng/mL.)
COVID 19 Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Coronavirus, Active Protein (Cat# AAA258088)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Pricing
5-Lipoxygenase, Active Protein (Cat# AAA14373)
Full Name
5-Lipoxygenase protein
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Pricing
Purity
(The purity of RIPK1 was determined to be >70% by densitometry, approx. MW 108 kDa.)
Urinary Trypsin Inhibitor, Active Protein (Cat# AAA10834)
Full Name
Human Urinary Trypsin Inhibitor (UTI)
Pricing
SDS-PAGE
COVID 19 Spike S1 Protein Coronavirus, Active Protein (Cat# AAA22156)
Full Name
Recombinant 2019-nCoV S1 Protein
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
Aspartate Aminotransferase, Active Protein (Cat# AAA11475)
Full Name
Glutamate Oxaloacetate Transmainase
Gene Names
GOT1; cCAT; CASPAT
Pricing
SDS-PAGE
(SDS-PAGE of ~14 kDa Active Mouse Recombinant Alpha Synuclein Protein Aggregate. Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate (2 ug).)
Alpha Synuclein, Active Protein (Cat# AAA253925)
Full Name
Alpha Synuclein Protein
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Applications
Western Blot
Purity
>95%
Ion-exchange Purified
Ion-exchange Purified
Pricing
GDF11, Active Protein (Cat# AAA14381)
Full Name
GDF11 protein
Gene Names
GDF11; BMP11; BMP-11
Purity
> 98% by SDS-PAGE analysis and HPLC
Pricing
CD22, Active Protein (Cat# AAA14393)
Full Name
CD22 protein
Gene Names
CD22; SIGLEC2; SIGLEC-2
Purity
> 98% by SDS-PAGE and HPLC analyses
Pricing
Application Data
(Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is 0.2-1 ng/mL.)
Growth Hormone, Active Protein (Cat# AAA258048)
Full Name
Growth Hormone Protein, Human, Recombinant
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Purity
>90% as determined by SDS-PAGE
Pricing
Parathyroid Hormone, Active Protein (Cat# AAA10826)
Full Name
Recombinant Human Parathyroid Hormone (1-84)
Gene Names
PTH; PTH1
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
Pricing
Vascular Endothelial Growth Factor-E, Active Protein (Cat# AAA10810)
Full Name
Recombinant Vascular Endothelial Growth Factor-E (Orf Virus)
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
Bioactivity
(Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) is a member of the TGF beta protein family. Myostatin is a secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MSTN with BMP1.)
Myostatin, Active Protein (Cat# AAA150112)
Full Name
Active Myostatin (MSTN)
Gene Names
MSTN; GDF8; MSLHP
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human)
Neutrophil gelatinase-associated lipocalin (NGAL), Active Protein (Cat# AAA153086)
Full Name
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Gene Names
Lcn2; 24p3; Sip24; AW212229
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Insulin, Active Protein (Cat# AAA10812)
Full Name
Recombinant Human Insulin
Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
Purity
Greater than 98.0% as determined by RP-HPLC analysis.
Pricing
Application Data
Myostatin (MSTN), Active Protein (Cat# AAA148194)
Full Name
Active Myostatin (MSTN)
Gene Names
MSTN; GDF8; MSLHP
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Dipeptidyl-Peptidase 4, Active Protein (Cat# AAA10854)
Full Name
Recombinant Human Dipeptidyl-Peptidase 4
Gene Names
DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
Purity
Greater than 95.0% as determined by Analysis by SDS-PAGE.
Pricing
Interferon-beta 1a, Active Protein (Cat# AAA10808)
Full Name
Recombinant Human Interferon-beta 1a
Gene Names
IFNB1; IFB; IFF; IFNB
Purity
Greater than 99.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
Hepatocyte Growth Factor, Active Protein (Cat# AAA10809)
Full Name
Recombinant Human Hepatocyte Growth Factor, Sf9
Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
SDS-PAGE
(The purity of PAD4 was determined to be >95% by densitometry. Approx. MW 96 kDa.)
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150562)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Gene Names
Ace2; 2010305L05Rik
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
Monkeypox virus/MPXV A29L, Active Protein (Cat# AAA18896)
Full Name
Recombinant Monkeypox virus/MPXV A29L Protein, C-His
Applications
Western Blot
Purity
>90% by SDS-PAGE.
Pricing
Beta Lactamase, Active Protein (Cat# AAA10821)
Full Name
Recombinant Beta Lactamase
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Elcatonin, Active Protein (Cat# AAA10849)
Full Name
Elcatonin
Purity
Greater than 93.3% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
IFN ALPHA, Active Protein (Cat# AAA12301)
Full Name
RECOMBINANT CHICKEN INTERFERON ALPHA
Gene Names
IFNA3; IFNA; IFNA1; IFNA2; IFNA6; IFN-alpha; IFN-gamma
Applications
Functional Assay
Purity
>85% by Ni chelate chromatography
Pricing
Bioactivity
(E-Cadherin, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. As E-cadherin has the function of cell adhesion, we measure the activity of recombinant human E-cadherin by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When 5 x 104 cells/well are added to different concentrations of recombinat human E-cadherin coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of MCF7 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.23 ug/ml.)
E-cadherin, Active Protein (Cat# AAA161657)
Full Name
Active E-cadherin
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM6 at 2 ?g/mL can bind Anti- CEACAM5/CEACAM6 recombinant antibody, the EC50 is 0.9430-1.377 ng/mL.)
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), Active Protein (Cat# AAA279203)
Full Name
Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6)(Active)
Gene Names
CEACAM6; NCA; CEAL; CD66c
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
HIV1 tat, Active Protein (Cat# AAA14384)
Full Name
HIV1 tat protein
Applications
Western Blot
Purity
> 99% by SDS-PAGE and HPLC
Pricing
Application Data
Annexin A1, Active Protein (Cat# AAA150136)
Full Name
Active Annexin A1 (ANXA1)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing
L-Asparaginase, Active Protein (Cat# AAA10818)
Full Name
L-Asparaginase
Gene Names
iaaA; ECK0818; JW0812; spt; ybiK
Purity
Greater than 96% as determined by SDS-PAGE.
Pricing
Sequence
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150976)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Gene Names
ACE2; ACEH
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant chicken RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >50 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161804)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Gene Names
RBP4A; RBP; RBP4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Chorionic Gonadotropin, Active Protein (Cat# AAA10824)
Full Name
Human Chorionic Gonadotropin
Gene Names
CGB; CGB3; CGB5; CGB7; CGB8; hCGB
Pricing
Bioactivity
(Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant rat antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 ug/ml in the assay buffer and 10 ul different concentrations of recombinant rat antithrombin III (MW: 52.6 KD) was incubated with 10 ul diluted thrombin at 37 degree C for 30 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat antithrombin III significantly decreased thrombin activity. The inhibition IC50 was )
Antithrombin (AT), Active Protein (Cat# AAA161887)
Full Name
Active Antithrombin (AT)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors. Human growth hormone is a 191 amino acid single-chain polypeptide produced via the anterior pituitary of the brain in the acidophilic, somatotrophic cells. Its production is tightly regulated through several complex feedback mechanisms in response to stress, exercise, nutrition, sleep, and growth hormone itself. Annexin A2 (ANXA2) has been shown to play multiple roles in growth, development, metabolism and ANXA2 is a high affinity receptor for GH. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GH and recombinant human ANXA2. Briefly, GH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ANXA2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GH and recombinant human ANXA2 was shown in Figure 1, the EC50 for this effect is 2.1 ug/mL.)
Growth Hormone (GH), Active Protein (Cat# AAA161667)
Full Name
Active Growth Hormone (GH)
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Sample: Recombinant BMP7, Mouse;Antibody: Rabbit Anti-Mouse BMP7 Ab)
Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA21057)
Full Name
Active Bone Morphogenetic Protein 7 (BMP7)
Gene Names
Bmp7; OP1
Reactivity
Mus musculus (Mouse)
Applications
Cell Culture, Activity Assay
Purity
>98%
Pricing
Bioactivity
(Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspase 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. Thus the activity of recombinant mouse caspase 3 can be measuered by calculating the concentration of the pNA released from the substrate. The reaction was performed in adding 50 ul 2×buffer (50mM HEPES,100mM NaCl,10mM DTT, 2mM EDTA, 10% glycerol) to 96 well plates, then add 50 ul various concentrations of caspase 3 (diluted by 1×buffer, 25mM HEPES, 50mM NaCl, 5mM DTT, 1mM EDTA, 5% glycerol) to each well, finally, add 5 ul 4mM Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37 degree C for 1h. p-Nitroaniline (pNA) Standard curve prepare by double dilute 200 uM pNA with 1×buffer and record the OD value at 405 nm. The specific activity of recombinant mouse caspase3 is >3000 pmol/min/ug.)
Caspase 3 (CASP3), Active Protein (Cat# AAA161756)
Full Name
Active Caspase 3 (CASP3)
Gene Names
Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Hirudin, Active Protein (Cat# AAA10836)
Full Name
Recombinant Hirudin
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant human CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161776)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Tissue Plasminogen Activator, Active Protein (Cat# AAA10814)
Full Name
Recombinant Human Tissue Plasminogen Activator
Gene Names
PLAT; TPA; T-PA
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
Bioactivity
(Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant pig AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant pig AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant pig AP is >600 U/mg.)
Aprotinin (AP), Active Protein (Cat# AAA161813)
Full Name
Active Aprotinin (AP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Sequence
Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161902)
Full Name
Active Carbonic Anhydrase IV (CA4)
Gene Names
Car4; Ca4
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.