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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2650-2700 of 2875 product results
Purity
(The purity of LRRK2 (I2020T) was determined to be >70% by densitometry, approx. MW 210 kDa.)
LRRK2, Active Protein (Cat# AAA14255)
Full Name
LRRK2 Mutant (I2020T), Active
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Purity
>70%
Pricing
Bioactivity
(CD40, also known as Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5), is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transm)
Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40), Active Protein (Cat# AAA153079)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40)
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Figure 2. SDS-PAGESample: Active recombinant VDR, Human)
Vitamin D Receptor (VDR), Active Protein (Cat# AAA148172)
Full Name
Active Vitamin D Receptor (VDR)
Gene Names
VDR; NR1I1; PPP1R163
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Pepsinogen I, Active Protein (Cat# AAA11504)
Full Name
Pepsinogen I (PGI/PGA), Human Stomach
Purity
> 90% (SDS-PAGE, may contain minor cleavage products)
Pricing
Bioactivity
(Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant human antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 ug/ml in the assay buffer and 10 ul different concentrations of recombinant human antithrombin III (MW: 50 KD) was incubated with 10 ul diluted thrombin at 37 degree C for 30 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human antithrombin III significantly decreased thrombin activity. The inhibition IC50 was )
Antithrombin (AT), Active Protein (Cat# AAA161886)
Full Name
Active Antithrombin (AT)
Gene Names
SERPINC1; AT3; AT3D; ATIII; THPH7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
ELISA
(Fig 2: Binding of VEGF-A isoforms to recombinant human soluble Neuropilin-1 receptor (NRP-1) in a functional ELISA. NRP-1 was coated with 1ug/ml (100ul/well), the ligands were added with 10ng/ml.)
NRP-1, soluble, Active Protein (Cat# AAA14949)
Full Name
Recombinant Human Soluble Neuropilin-1
Gene Names
NRP1; NP1; NRP; BDCA4; CD304; VEGF165R
Purity
>98% by SDS-PAGE & Coomassie stain
Pricing
Bioactivity
(Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma.It may also play a role in liver development or regeneration. Besides, Tau Protein (MAPT) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human MAPT. Briefly, ITIH4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MAPT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of ITIH4 and MAPT was shown in Figure 1, the EC50 for this effect is 0.18 ug/mL.)
Inter Alpha-Globulin Inhibitor H4 (ITIH4), Active Protein (Cat# AAA161928)
Full Name
Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)
Gene Names
ITIH4; H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Application Data
(3CL Proteinase inhibitor screening using 2019-nCoV 3CL MPro Recombinant Protein Provided by customer.)
COVID 19 3CL MPro Coronavirus, Active Protein (Cat# AAA27953)
Full Name
2019-nCoV 3CL MPro Recombinant Protein
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Kallikrein 7, Active Protein (Cat# AAA174597)
Full Name
Recombinant Human Kallikrein 7/KLK7 Protein (His Tag)
Gene Names
KLK7; hK7; SCCE; PRSS6
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Galectin 9 (GAL9) is a member of the beta-galactoside-binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused o)
Galectin 9 (GAL9), Active Protein (Cat# AAA153026)
Full Name
Active Galectin 9 (GAL9)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissuenonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant human ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 (assay buffer), ainitiated by addition 50 uL of 0.625 ug/ml ALPL (diluted by assay buffer) to 50 uL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human ALPL is >11000 pmol/min/ug.)
Alkaline Phosphatase, Tissue-nonspecific (ALPL), Active Protein (Cat# AAA161829)
Full Name
Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A1+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A1+B2+G2), Active Protein (Cat# AAA60318)
Full Name
Recombinant AMPK Complex (A1+B2+G2) protein
Purity
>=90%
Pricing
Bioactivity
(Activating transcription factor 3 (ATF3) is a stress-induced transcription factor that plays vital roles in modulating metabolism, immunity and oncogenesis. ATF3 acts as a hub of the cellular adaptive-response network. Multiple extracellular signals, such as endoplasmic reticulum (ER) stress, cytokines, chemokines, and LPS, are connected to ATF3 induction. Proto-oncogene c-Fos can bind to ATF3 to regulate signal transduction, cell proliferation and differentiation. Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse ATF3 and recombinant human FOS. Briefly, ATF3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FOS-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ATF3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse ATF3 and recombinant human FOS was shown in Figure 1, the EC50 for this effect is 0.13 ug/mL.)
Activating Transcription Factor 3 (ATF3), Active Protein (Cat# AAA161885)
Full Name
Active Activating Transcription Factor 3 (ATF3)
Gene Names
Atf3; LRG-21
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant human Annexin V-GFP and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 1), the combination of Annexin V-GFP and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-GFP can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)
Annexin V (ANXA5), Active Protein (Cat# AAA161724)
Full Name
Active Annexin V (ANXA5)
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Glypican 1 (GPC1) is one of the six glycosylphosphatidylinositol-anchored, cell surface heparan sulfate proteoglycans that acts as a growth factor signaling. Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells. The function of GPC-1 is complex and varied, it can modulate various signaling pathways including Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), fibroblast growth factors (FGF), vascular endothelial growth factor-A (VEGF-A), transforming growth factor-beta (TGF-beta), Wnt, Hedgehog (Hh), and bone morphogenic protein (BMP) through specific interactions with pathway ligands and receptors. Besides, Syndecan 4 (SDC4) has been also identified as an interactor of GPC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC1 and recombinant chicken SDC4. Briefly, GPC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SDC4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse GPC1 and recombinant chicken SDC4 was shown in Figure 1, the EC50 for this effect is 2.5 ug/mL.)
Glypican 1 (GPC1), Active Protein (Cat# AAA161826)
Full Name
Active Glypican 1 (GPC1)
Gene Names
Gpc1; AI462976
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag), the EC50 of SARS-CoV-2 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag) is 100-700ng/mL.)
COVID 19 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag) Coronavirus, Active Protein (Cat# AAA258078)
Full Name
SARS-CoV-2 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
HIV-1 env gp120, Strain YU2, Active Protein (Cat# AAA14753)
Full Name
HIV-1 env gp120, Strain YU2 (M Tropic), Recombinant (Human Immunodeficiency Virus-1)
Applications
Western Blot
Purity
>95% (SDS-PAGE, reduced). Purified by immunoaffinity chromatography.
Pricing
Bioactivity
(Galectin 8 (GAL8), also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Bovine GAL8 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL8 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL8, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL8 is 0.781 ug/mL.)
Galectin 8 (GAL8), Active Protein (Cat# AAA152177)
Full Name
Active Galectin 8 (GAL8)
Gene Names
LGALS8; galectin-8
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant GZMM, Human;Antibody: Rabbit Anti-Human GZMM Ab)
Granzyme M (GZMM), Active Protein (Cat# AAA21105)
Full Name
Active Granzyme M (GZMM)
Gene Names
GZMM; MET1; LMET1
Reactivity
Homo sapiens (Human)
Applications
Cell Culture, Activity Assay
Purity
>92%
Pricing
Application Data
(Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/ml.)
IL-15 Receptor alpha & IL-15 Fusion Protein/IL15RA&IL15, Active Protein (Cat# AAA22119)
Full Name
Recombinant Human IL-15 Receptor alpha & IL-15 Fusion Protein/IL15RA&IL15 Protein (C-Fc)
Gene Names
IL15RA; CD215
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
CD200 Receptor 1, Active Protein (Cat# AAA22128)
Full Name
Recombinant Human CD200 Receptor 1 Protein
Gene Names
CD200R1; OX2R; MOX2R; CD200R; HCRTR2
Purity
> 95% as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)
Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161665)
Full Name
Active Colony Stimulating Factor 3, Granulocyte (GCSF)
Gene Names
CSF3; G-CSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Aryl Hydrocarbon Receptor (AhR), also known as bHLHE76, is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. It is a ligand-activated transcription factor best known for its role in sensing xenobiotics and regulating the expression of genes involved in xenobiotic metabolism, including drug transporters and drug metabolizing enzymes. Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) has been identified as an interactor of AhR, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AhR and recombinant human HSP90aA1. Briefly, AhR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HSP90aA1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AhR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of AhR and HSP90aA1 was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 for this effect is 0.09 ug/mL.)
Aryl Hydrocarbon Receptor (AhR), Active Protein (Cat# AAA161844)
Full Name
Active Aryl Hydrocarbon Receptor (AhR)
Gene Names
AHR; bHLHe76
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Figure 1.Cell proliferation of ECV304 cells after stimulated with VEGFA.)
Vascular Endothelial Growth Factor A, Active Protein (Cat# AAA150078)
Full Name
Active Vascular Endothelial Growth Factor A (VEGFA)
Gene Names
VEGFA; VEGF
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Apolipoprotein A5 (APOA5) is a small protein, expressed predominantly in the liver. It has been identified to play an important role in lipid metabolism, specifically in triglyceride (TG) and TG-rich lipoproteins (TRLs) metabolism. Apolipoprotein C3 (APOC3) has been proved to be one of the ligands of APOA5. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOA5 and recombinant human APOC3. Briefly, APOA5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOC3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOA5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human APOA5 and recombinant human APOC3 was shown in Figure 1, the EC50 for this effect is 0.14 ug/mL.)
Apolipoprotein A5 (APOA5), Active Protein (Cat# AAA161872)
Full Name
Active Apolipoprotein A5 (APOA5)
Gene Names
APOA5; RAP3; APOAV
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Amine Oxidase, Copper Containing 3 (AOC3) is a copper amine oxidase with a topaquinone cofactor. AOC3 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. AOC3 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. The enzyme is sensitive to inhibition by semicarbazide. AOC3 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver. The activity of recombinant mouse AOC3 was measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of AOC3 (diluted by assay buffer) to 50 ul substrate mixture of 2 mM Benzylamine, 2 units/mL HRP and 100 uM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.)
Amine Oxidase, Copper Containing 3 (AOC3), Active Protein (Cat# AAA161883)
Full Name
Active Amine Oxidase, Copper Containing 3 (AOC3)
Gene Names
Aoc3; SSAO; VAP1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 ug/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 ug/ml.)
CD47, Active Protein (Cat# AAA258008)
Full Name
CD47 Protein, Human, Recombinant (ECD, hFc Tag)
Gene Names
CD47; IAP; OA3; MER6
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
CD55/DAF, Active Protein (Cat# AAA27760)
Full Name
CD55/DAF Protein, Human, Recombinant (His Tag)
Gene Names
CD55; CR; TC; DAF; CROM
Purity
> 95% as determined by SDS-PAGE
> 90% as determined by SEC-HPLC
> 90% as determined by SEC-HPLC
Pricing
Angiopoietin-2 / Ang-2, Active Protein (Cat# AAA14953)
Full Name
Human Angiopoietin-2 / Ang-2
Gene Names
VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
Reactivity
Human
Applications
Immunoblot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Sandwich ELISA
Purity
> 95%, by SDS-PAGE and HPLC analysis
Pricing
Glycerol-3-phosphate dehydrogenase, Active Protein (Cat# AAA11456)
Full Name
Glycerol-3-phosphate Dehydrogenase (GPDH), Rabbit Muscle
Pricing
Bioactivity
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide r)
Superoxide Dismutase 2, Mitochondrial (SOD2), Active Protein (Cat# AAA153068)
Full Name
Active Superoxide Dismutase 2, Mitochondrial (SOD2)
Gene Names
Sod2; MnSOD; Sod-2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Monocyte Chemotactic Protein 1 (MCP1), also known as C-C motif chemokine 2(CCL2), is a member of the beta (C-C) subfamily of chemokines that is a chemoattractant for monocytes and basophils. Mouse CCL2 is secreted as a 14 kDa glycoprotein monomer but nonc)
Monocyte Chemotactic Protein 1 (MCP1), Active Protein (Cat# AAA152975)
Full Name
Active Monocyte Chemotactic Protein 1 (MCP1)
Gene Names
Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
Programmed Cell Death 1 Ligand 1, Active Protein (Cat# AAA174604)
Full Name
Recombinant Mouse Programmed Cell Death 1 Ligand 1 Protein
Gene Names
Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
Application Data
(Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The EC50 for this effect is typically 2-14 ng/mL.)
FGF10, Active Protein (Cat# AAA257898)
Full Name
FGF10 Protein, Human, Recombinant
Purity
>95% as determined by SDS-PAGE
Pricing
Hazard Indicator
Flagellin, Active Protein (Cat# AAA14744)
Full Name
Flagellin (FliC)
Purity
Purified
>90% (SDS-PAGE)
>90% (SDS-PAGE)
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Acid phosphatase 1, Active Protein (Cat# AAA48522)
Full Name
Acid phosphatase 1,1-158aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
ACP1; HAAP; LMWPTP; LMW-PTP
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 3. The sandard curve of Dns-Tyr-Val-NH2)
Peptidylglycine Alpha Amidating Monooxygenase (PAM), Active Protein (Cat# AAA161894)
Full Name
Active Peptidylglycine Alpha Amidating Monooxygenase (PAM)
Gene Names
PAM; PAL; PHM
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Figure 3. Western BlotSample: Recombinant SOD3, Mouse;Antibody: Rabbit Anti-Mouse SOD3 Ab)
Superoxide Dismutase 3, Extracellular, Active Protein (Cat# AAA150073)
Full Name
Active Superoxide Dismutase 3, Extracellular (SOD3)
Gene Names
Sod3; EC-SOD; AI314465
Reactivity
Mus musculus (Mouse)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS-PAGE
(10% SDS-PAGE Coomassie staining. Lane 1: 3ug F4600-21. Lane 2: Protein Marker.)
Flt3, active, N-terminal His tag Receptor Tyrosine Kinase, Active Protein (Cat# AAA14760)
Full Name
Flt3, active, Recombinant, Human, N-terminal His tag Receptor Tyrosine Kinase (Fms-like Tyrosine Kinase 3, CD135)
Gene Names
Fiz1; AI790204; mFLJ00416
Reactivity
Human
Purity
> 80% by SDS-PAGE.
Pricing
Application Data
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1), Active Protein (Cat# AAA148210)
Full Name
Active Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Apolipoprotein A4 (APOA4) is a lipid-binding protein, which is primarily synthesized in the small intestine, packaged into chylomicrons, and secreted into intestinal lymph during fat absorption. In the circulation, apoA-IV is present on chylomicron remnants, high-density lipoproteins, and also in lipid-free form. ApoA-IV is involved in a myriad of physiological processes such as lipid absorption and metabolism, anti-atherosclerosis, platelet aggregation and thrombosis, glucose homeostasis and food intake. Besides, Apolipoprotein H (APOH) has been identified as an interactor of APOA4, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOA4 and recombinant bovine APOH. Briefly, APOA4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOH-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human APOA4 and recombinant bovine APOH was shown in Figure 1, the EC50 for this effect is 0.16 ug/mL.)
Apolipoprotein A4 (APOA4), Active Protein (Cat# AAA161870)
Full Name
Active Apolipoprotein A4 (APOA4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Canine IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range )
IL-21, Active Protein (Cat# AAA48379)
Full Name
Recombinant canine IL-21 protein
Gene Names
IL21; IL-21
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.)
Galectin 2 (GAL2), Active Protein (Cat# AAA153012)
Full Name
Active Galectin 2 (GAL2)
Gene Names
Lgals2; AI324147; 2200008F12Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177979)
Full Name
Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-Fc)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
(Plasminogen activators are serine proteases that catalyze the activation of plasmin via proteolytic cleavage of its zymogen form plasminogen. Plasmin is an important factor in fibrinolysis, the breakdown of fibrin polymers formed during blood clotting. Th)
Tissue Plasminogen Activator (tPA), Active Protein (Cat# AAA153036)
Full Name
Active Tissue Plasminogen Activator (tPA)
Gene Names
PLAT; TPA; T-PA
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Ephrin-A4, also known as EFNA4 and EFL-4, is a member of the ligand of the EPH family. It is mainly expressed in the spleen, lymph nodes, ovary, small intestine, and colon of adults, as well as in the heart, lungs, liver, and kidneys of the fetus. It is involved in the development of neurons, blood vessels, and epithelium by regulating cell migration, rejection, and adhesion. Ephrin-A4 has been shown to bind EphA1,EphA2, EphA3, EphA4, EphA5, EphA6, EphA7 and EphB1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse EFNA4 and recombinant mouse EPHA1. Briefly, EFNA4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EphA1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse EFNA4 and recombinant mouse EphA1 was shown in Figure 1, the EC50 for this effect is 0.06 ug/mL.)
Ephrin A4 (EFNA4), Active Protein (Cat# AAA161917)
Full Name
Active Ephrin A4 (EFNA4)
Gene Names
Efna4; Epl4; EFL-4; LERK-4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 1. The binding activity of BMP2 with NOG.)
Bone Morphogenetic Protein 2, Active Protein (Cat# AAA150048)
Full Name
Active Bone Morphogenetic Protein 2 (BMP2)
Gene Names
BMP2; BDA2; BMP2A; SSFSC
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells.The ED50 for this effect is typically 2.9 ng/ml.)
Stem Cell Factor (SCF), Active Protein (Cat# AAA161694)
Full Name
Active Stem Cell Factor (SCF)
Gene Names
Kitlg; Mgf; SCF; Kitl
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant bovine RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rbRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rbRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >190 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161803)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Angiotensin-Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA235619)
Full Name
Recombinant Human Angiotensin-Converting Enzyme 2 (ACE2), Partial
Gene Names
ACE2; ACEH
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.