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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2700-2750 of 2875 product results
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150561)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Gene Names
ACE2; ACEH
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Sample: Active recombinant AST, Human)
Aspartate Aminotransferase (AST), Active Protein (Cat# AAA152060)
Full Name
Active Aspartate Aminotransferase (AST)
Gene Names
GOT1; cCAT; GIG18; cAspAT; ASTQTL1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Alanine Aminotransferase, Active Protein (Cat# AAA11476)
Full Name
Glutamate Pyruvate Transaminase (GPT), Alanine Transaminase (ALT)
Pricing
Butyrylcholinesterase, Active Protein (Cat# AAA11470)
Full Name
Butyryl Cholinesterase
Gene Names
BCHE; E1; CHE1; CHE2
Pricing
Application Data
(SDS-page of 90kDa Hsp90beta protein (AAA17806))
Hsp90 beta, Active Protein (Cat# AAA17806)
Full Name
Recombinant Human HSP90 beta, native sequence
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation
Purity
Affinity Purified
Pricing
Application Data
VEGF206, Active Protein (Cat# AAA14948)
Full Name
Human VEGF206
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
>=75% by SDS-PAGE & Coomasie stain
Pricing
SDS-PAGE
COVID 19 Spike S1+S2 Protein (ECD) Coronavirus, Active Protein (Cat# AAA22159)
Full Name
Recombinant 2019-nCoV S1+S2 Protein (ECD, His Tag)
Purity
>90% as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(One unit of enzyme activity is defined as the 1 ug of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37 degree C. The specific activity of recombinant mouse CA4 is > 50 pmol/min/ug.)
Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161901)
Full Name
Active Carbonic Anhydrase IV (CA4)
Gene Names
Car4; Ca4; AW456718
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A1+B1+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A1+B1+G2), Active Protein (Cat# AAA60344)
Full Name
Recombinant AMPK Complex (A1+B1+G2) protein
Purity
>=80%
Pricing
Application Data
(Biological Acitivity: Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
Interleukin 6, Active Protein (Cat# AAA48546)
Full Name
Interleukin 6, 30-212aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant horse IL6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with IL6 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant horse IL6 for 72h. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of TF-1 cells, the EC50 was 1.3 ng/ml.)
Interleukin 6 (IL6), Active Protein (Cat# AAA161679)
Full Name
Active Interleukin 6 (IL6)
Gene Names
IL6; IL-6
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
TCN2, Active Protein (Cat# AAA22097)
Full Name
Recombinant Human TCN2 Protein (His tag)
Gene Names
TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
Purity
> 90 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Apolipoprotein C1 (APOC1), also known as Apo-CI; ApoC-I; apo-CIB; apoC-IB, is the smallest size apolipoprotein of all apolipoprotein C family (Mr = 6.6 kDa) and located at position 19q13.32. APOC1 is primarily expressed in the liver and activated when monocytes differentiate into macrophages. It plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. It has anti-inflammatory activity and also can promote the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Besides, Apolipoprotein C3 (APOC3) has been identified as an interactor of APOC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOC1 and recombinant mouse APOC3. Briefly, APOC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOC3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human APOC1 and recombinant mouse APOC3 was shown in Figure 1, the EC50 for this effect is 2.19 ug/mL.)
Apolipoprotein C1 (APOC1), Active Protein (Cat# AAA161719)
Full Name
Active Apolipoprotein C1 (APOC1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 (assay buffer), initiated by addition 50 ul of 3 ug/ml ACP1 (diluted by assay buffer) to 50 uL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 ul of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/ug.)
Acid Phosphatase 1 (ACP1), Active Protein (Cat# AAA161821)
Full Name
Active Acid Phosphatase 1 (ACP1)
Gene Names
Acp1; Acp-1; LMW-PTP; AI427468; 4632432E04Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measurements of kinetic parameters of Recombinant 2019-nCoV 3C-like Proteinase (CAT# AAA27964) (For reference only).)
COVID 19 3C-like Proteinase Coronavirus, Active Protein (Cat# AAA27964)
Full Name
Recombinant 2019-nCoV 3C-like Proteinase (N-6His) (CR76)
Purity
> = 95% as determined by reducing SDS-PAGE. Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Pricing
Bioactivity
(Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissuenonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant mouse ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 (assay buffer), ainitiated by addition 50 ul of 0.3 ug/ml ALPL (diluted by assay buffer) to 50 uL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse ALPL is >11000 pmol/min/ug.)
Alkaline Phosphatase, Tissue-nonspecific (ALPL), Active Protein (Cat# AAA161830)
Full Name
Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
Gene Names
Alpl; ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE)
Acid Sphingomyelinase (ASM), Active Protein (Cat# AAA152129)
Full Name
Active Acid Sphingomyelinase (ASM)
Gene Names
SMPD1; ASM; NPD; ASMASE
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177978)
Full Name
Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-10His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Apo-SAA1, Active Protein (Cat# AAA14945)
Full Name
Human Apo-SAA1
Gene Names
SAA1; SAA; PIG4; SAA2; TP53I4
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analyses
Pricing
Bioactivity
(Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant bovine AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant bovine AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant bovine AP is >2000 U/mg.)
Aprotinin (AP), Active Protein (Cat# AAA161810)
Full Name
Active Aprotinin (AP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 4a (C4a) is a component of the complement system, which is a cleavage product of the complement C4 protein. C4a has been implicated in various inflammatory and immune responses. It acts as a chemoattractant, recruiting immune cells such as neutrophils and macrophages to the site of inflammation. Additionally, C4a can stimulate the release of pro-inflammatory cytokines and chemokines, further amplifying the immune response. Besides, the binding of MASP2 to C4a is an important step in the lectin pathway of the complement system, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C4a and recombinant human MASP2. Briefly, C4a was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MASP2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C4a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse C4a and recombinant human MASP2 was shown in Figure 1, the EC50 for this effect is 0.21 ug/mL.)
Complement Component 4a (C4a), Active Protein (Cat# AAA161729)
Full Name
Active Complement Component 4a (C4a)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Myoglobin (symbol Mb or MB or MYO) is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates in general and in almost all mammals. It is related to hemoglobin, which is the iron- and oxygen-binding protein in blood, specifically in the red blood cells. Myoglobin is found in Type I muscle, Type II A and Type II B, but most texts consider myoglobin not to be found in smooth muscle. Besides, Proteasome 26S Subunit, Non ATPase 4 (PSMD4) has been identified as an interactor of MYO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MYO and recombinant human PSMD4 Briefly, MYO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to PSMD4-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-MYO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MYO and PSMD4 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MYO with PSMD4.)
Myoglobin, Active Protein (Cat# AAA150089)
Full Name
Active Myoglobin (MYO)
Gene Names
MB; PVALB
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Maltose binding protein (MBP) encoded by the malE gene in Escherichia coli, is a 44-kD monomeric periplasmic protein. MBP is component of the Escherichia coli maltose/maltodextrin system, which regulates the uptake and catabolism of maltrodextrins as part of the chemotactic response. This protein is used in recombinant protein expression as an affinity and solubility tag. Glutathione S Transferase Alpha 3 (GSTa3) has been identified as an interactor of MBP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant MBP and recombinant mouse GSTa3. Briefly, biotin-linked MBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GSTa3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant MBP and recombinant mouse GSTa3 was shown in Figure 1, the EC50 for this effect is 0.46 ug/mL.)
Maltose Binding Protein (MBP), Active Protein (Cat# AAA161942)
Full Name
Active Maltose Binding Protein (MBP)
Gene Names
malE; ECK4026; JW3994; malJ
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
Tumor necrosis factor ligand superfamily member 13B (TNFSF13B), Active Protein (Cat# AAA244036)
Full Name
Recombinant Human Tumor necrosis factor ligand superfamily member 13B (TNFSF13B), partial
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA146615)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
(FLT-1 Sandwich ELISA using recombinant human soluble FLT-1 as standard . Mouse anti-human FLT-1 #EWI was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection)
VEGFR-1/Flt-1 (native), soluble, Active Protein (Cat# AAA79126)
Full Name
Human VEGFR-1/Flt-1 (native), soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 10 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 1), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)
Annexin V (ANXA5), Active Protein (Cat# AAA161723)
Full Name
Active Annexin V (ANXA5)
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(The activity was determined by immobilized Spike Protein S1 binding with Human ACE2 in a functional ELISA assay, the ED50 was determined to be 4nm.)
COVID 19 Spike Protein S1 Coronavirus, Active Protein (Cat# AAA14436)
Full Name
2019 nCoV/COVID19 Spike protein S1 Recombinant-Fc
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A2+B2+G2) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G2) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A2+B2+G2), Active Protein (Cat# AAA60320)
Full Name
Recombinant AMPK Complex (A2+B2+G2) protein
Purity
>=90%
Pricing
Bioactivity
(Fibroblast Growth Factor 19 (FGF19) is a member of the fibroblast growth factor (FGF) family. FGF19 has important roles as a hormone produced in the ileum in response to bile acid absorption, regulates new bile acid synthesis, acting through the FGFR4/Klo)
Fibroblast Growth Factor 19 (FGF19), Active Protein (Cat# AAA153122)
Full Name
Active Fibroblast Growth Factor 19 (FGF19)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Spike glycoprotein (S), Active Protein (Cat# AAA244032)
Full Name
Recombinant Human Novel Coronavirus Spike glycoprotein (S), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Alkaline Phosphatase, Active Protein (Cat# AAA11489)
Full Name
Alkaline Phosphatase (ALP), Porcine Kidney
Pricing
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 20-80 ng/mL in the presence of 10 ng/mL Recombinant Human Activin A.)
Follistatin, Active Protein (Cat# AAA258012)
Full Name
Follistatin Protein, Human, Recombinant (His Tag)
Gene Names
FST; FS
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
IL-1 beta, Active Protein (Cat# AAA79215)
Full Name
Human IL-1 beta
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
WB (Western Blot)
(Sample: Recombinant DPP4, Human;Antibody: Rabbit Anti-Human DPP4 Ab)
Dipeptidyl Peptidase IV (DPP4), Active Protein (Cat# AAA149244)
Full Name
Active Dipeptidyl Peptidase IV (DPP4)
Gene Names
DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
Reactivity
Homo sapiens (Human)
Applications
Activity Assay
Purity
>90%
Pricing
Application Data
(Type 1 alpha synuclein preformed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers . Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer , as compared to when 10 uM of Type 2 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer or 100 uM of alpha Synuclein monomer . Thioflavin T ex = 450 nm, em = 485 nm.)
Alpha Synuclein, Active Protein (Cat# AAA103871)
Full Name
Alpha Synuclein Protein
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Applications
Western Blot
Purity
Ion-exchange Purified
Pricing
SDS-PAGE
Interleukin-36 alpha protein (Il36a), Active Protein (Cat# AAA114251)
Full Name
Recombinant Mouse Interleukin-36 alpha protein (Il36a) (Active)
Gene Names
Il1f6; Fil1; Il1f9; Il36a; IL-1H1; IL1RP2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse ST2-His at 10ug/mL (100uL/well) can bind human TMED1-Fc, the EC50 of human TMED1-Fc is 0.05-0.2ug/mL.)
TMED1, Active Protein (Cat# AAA258019)
Full Name
TMED1 Protein, Human, Recombinant (hFc Tag)
Gene Names
TMED1; Tp24; Il1rl1l; IL1RL1LG
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A2+B2+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A2+B2+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 ul ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A2+B2+G1), Active Protein (Cat# AAA60319)
Full Name
Recombinant AMPK Complex (A2+B2+G1) protein
Purity
>=90%
Pricing
SDS-PAGE
Annexin A2 (ANXA2), Active Protein (Cat# AAA149253)
Full Name
Active Annexin A2 (ANXA2)
Gene Names
Anxa2; Cal1h; AW215814
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Thymosin-Beta 4 (TMSB4X), Active Protein (Cat# AAA214284)
Full Name
Recombinant Human Thymosin-Beta 4 (TMSB4X)
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.5-3 ug/mL in the presence of 10 ng/ml Recombinant Human ctivin A.)
Follistatin, Active Protein (Cat# AAA257910)
Full Name
Follistatin Protein, Human, Recombinant (hFc Tag)
Gene Names
FST; FS
Purity
>85% as determined by SDS-PAGE
Pricing
Myeloperoxidase, Active Protein (Cat# AAA11485)
Full Name
Myeloperoxidase (MPO), Human Neutrophil
Purity
> 96% (SDS-PAGE)
Pricing
Bioactivity
(C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Ficolin 2 (FCN2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CRP and recombinant mouse FCN2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FCN2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human CRP and recombinant mouse FCN2 was shown in Figure 1, the EC50 for this effect is 0.008 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161782)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Apo-SAA, Active Protein (Cat# AAA14951)
Full Name
Human Apo-SAA
Gene Names
SAA1; SAA; PIG4; SAA2; TP53I4
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analyses
Pricing
Bioactivity
Angiotensin-converting enzyme (ACE2), Active Protein (Cat# AAA244034)
Full Name
Recombinant Macaca fascicularis Angiotensin-converting enzyme (ACE2), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant bovine AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant bovine AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant bovine AP is >3000 U/mg.)
Aprotinin (AP), Active Protein (Cat# AAA161811)
Full Name
Active Aprotinin (AP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Biological Activity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range )
Growth Hormone, 20kD Variant, Active Protein (Cat# AAA14756)
Full Name
Growth Hormone, Recombinant, Human, 20kD Variant (hGH)
Highly Purified
Highly Purified
Applications
SDS-PAGE, Bioactivity
Purity
> 95% by SDS-PAGE
Pricing
Application Data
(Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family.It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control.Result: Gelatin hydrolysis by recombinant mouse MMP2 was shown in figure 1.Figure 1. Hydrolysis of gelatin by recombinant mouse MMP2.)
Matrix Metalloproteinase 2, Active Protein (Cat# AAA150070)
Full Name
Active Matrix Metalloproteinase 2 (MMP2)
Gene Names
Mmp2; 2-MMP; anon-WO0118547.84; CG1794; dm-2MMP; Dm2-MMP; DmelCG1794; dmmp2; dMMP2; l(2)02353; mmp2; MMP2
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150563)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.