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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2600-2650 of 2875 product results
WB (Western Blot)
(Western Blot: Spike RBD HIS Tag detected with Anti-HIS-Tag antibody HRP conjugate showing a band at approx. 30 kDa)
COVID 19 Spike Glycoprotein S1 RBD Coronavirus, Active Protein (Cat# AAA13473)
Full Name
Recombinant Human SARS-CoV-2 Spike Glycoprotein S1 RBD (HEK)
Purity
Affinity chromatography. Purity: >95% by SDS PAGE gel
Pricing
Bioactivity
(Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)
Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153089)
Full Name
Active Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(WNT3 Is a Functional Biomarker for Predicting the DE Differentiation Potential (A) WNT3 levels in control and various knockdown HUES8 sublines correlate with their DE differentiation efficiency.The four protocols included the following treatments in a serum-free medium: (1) A - > A (Activin A only for 4 days); (2) AW - > A (Activin A and Wnt3a for 2 days followed by Activin A only for another 2 days); (3) A - > AX (Activin A only for 2 days followed by Activin A and XAV 939 for another 2 days); and (4) AW - > AX (Activin A and Wnt3a for 2 days followed by Activin A and XAV 939 for another 2 days).)
Activin, Active Protein (Cat# AAA76389)
Full Name
Human Activin Recombinant (CHO Cell)
Gene Names
INHBA; EDF; FRP
Purity
>97%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, Balb/c 3T3 cells were seeded into triplicate wells of 96-well plates and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant human EGF. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Balb/c 3T3 cells after incubation with rhEGF for 48h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhEGF was shown in Figure2. It was obvious that rhEGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 3.9 ng/ml.)
Epidermal Growth Factor (EGF), Active Protein (Cat# AAA161747)
Full Name
Active Epidermal Growth Factor (EGF)
Gene Names
EGF; URG; HOMG4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Elastase, Active Protein (Cat# AAA11482)
Full Name
Elastase, Porcine Pancreas
Gene Names
CELA1; ELA1; ELS1; Elastase-1
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153046)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3, Active Protein (Cat# AAA177967)
Full Name
Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of rmTPO on MO7e cells)
Thrombopoietin (TPO), Active Protein (Cat# AAA161704)
Full Name
Active Thrombopoietin (TPO)
Gene Names
Thpo; Ml; Tpo; Mgdf; Tpo1; Tpo2; Tpo3; Tpo4; Mpllg
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(CLEC3B (Ctype lectin domain family 3 member B), also known as Tetranectin (TN), is a plasminogen-kringle-4-binding protein that is detected in various endocrine tissues and in epithelial and mesenchymal cells, including fibroblasts, monocytes, and neutrophils. Studies have shown that abnormal CLEC3B expression plays an important role in tumourigenesis and metastasis in gastric adenocarcinoma, breast cancer, cervical cancer, ovarian cancer, carcinoma of the urinary bladder, and melanoma. Besides, Secreted Phosphoprotein 2 (SPP2) has been identified as an interactor of CLEC3B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SPP2 and recombinant human CLEC3B. Briefly, CLEC3B was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SPP2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CLEC3B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CLEC3B and recombinant human SPP2 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.)
C-Type Lectin Domain Family 3, Member B (CLEC3B), Active Protein (Cat# AAA161934)
Full Name
Active C-Type Lectin Domain Family 3, Member B (CLEC3B)
Gene Names
CLEC3B; TN; TNA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The chemotactic effect of ENA-78 on THP-1 cells)
Epithelial Neutrophil Activating Peptide 78 (ENA78), Active Protein (Cat# AAA153063)
Full Name
Active Epithelial Neutrophil Activating Peptide 78 (ENA78)
Gene Names
CXCL5; SCYB5; ENA-78
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), is a divergent member of the Transforming Growth Factor beta (TGF-beta) superfamily. Human GDF-15 shares 66% and 68% amino acid sequence identity with the rat and mouse proteins, respectively. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF15 has been shown to have various functions, including inhibition of Tumor Necrosis Factor alpha (TNF-alpha) production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. A functional binding ELISA assay was conducted to detect the interaction of recombinant rat GDF-15 and recombinant human GP9. Briefly, GDF-15 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GP9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GDF-15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GDF-15 and GP9 was shown in Figure 1, the EC50 for this effect is 0.47 ug/mL.)
Growth Differentiation Factor 15 (GDF15), Active Protein (Cat# AAA161875)
Full Name
Active Growth Differentiation Factor 15 (GDF15)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the TNF family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. A functional ELISA assay was conducted to detect the interaction of recombinant human FASL and recombinant human FAS. Briefly, FASL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FAS-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FASL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FASL and recombinant human FAS was shown in Figure 1, the EC50 for this effect is 0.044 ug/mL.)
Factor Related Apoptosis Ligand (FASL), Active Protein (Cat# AAA161660)
Full Name
Active Factor Related Apoptosis Ligand (FASL)
Gene Names
FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
SDS-PAGE
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA148249)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Reactivity
Rat
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Bioactivity
SARS-CoV Spike Protein, Active Protein (Cat# AAA177982)
Full Name
Recombinant SARS-CoV Spike Protein (RBD, mFc Tag)(Active)
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(The epidermal growth factor receptor (EGFR) is a growth factor receptor that induces cell differentiation and proliferation upon activation through the binding of one of its ligands. The receptor is located at the cell surface, where the binding of a ligand activates a tyrosine kinase in the intracellular region of the receptor. This tyrosine kinase phosphorylates a number of intracellular substrates that activates pathways leading to cell growth, DNA synthesis and the expression of oncogenes such as fos and jun. Epidermal growth factor (EGF) family peptides are ligands for the EGF receptor (EGFR), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EGFR and recombinant human EGF. Briefly, biotin-linked EGFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EGF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human EGFR and recombinant human EGF was shown in Figure 1, the EC50 for this effect is 11.46 ug/mL.)
Epidermal Growth Factor Receptor (EGFR), Active Protein (Cat# AAA161774)
Full Name
Active Epidermal Growth Factor Receptor (EGFR)
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153094)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Activity
(The activity was determined by immobilized Spike Protein S1 binding with Human ACE2 in a functional ELISA assay, the ED50 was determined to be 0.17nM.)
COVID 19 Spike S RBD Coronavirus, Active Protein (Cat# AAA14434)
Full Name
2019 nCoV/COVID19 Spike S RBD Recombinant
Purity
>90% by SDS-PAGE.
Pricing
Application Data
(BMP-2 BioLISA using recombinant human soluble BMPR-IA for capturing and recombinant human BMP-2 as standard. A rabbit anti-human BMP-2 antibody in combination with an goat anti-rabbit Biotin antibody was used for detection.)
BMP receptor-1A, soluble, Active Protein (Cat# AAA79169)
Full Name
Human BMP receptor-1A, soluble
Gene Names
BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Gelsolin (GSN), one of the most abundant actin-binding proteins, is a multifunctional regulator of physiological and pathological cellular process and regulate cell migration, cell morphology, proliferation and apoptosis. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm and a previous study revealed that the levels of GSN are decreased in various cancer. Profilin-1 (PFN1) can also bind to actin and affects the structure of the cytoskeleton. PFN1 is one of the ligands of GSN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GSN and recombinant human PFN1. Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PFN1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GSN and recombinant human PFN1 was shown in Figure 1, the EC50 for this effect is 1.11 ug/mL.)
Gelsolin (GSN), Active Protein (Cat# AAA161725)
Full Name
Active Gelsolin (GSN)
Gene Names
GSN; ADF; AGEL
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 1), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)
Annexin V (ANXA5), Active Protein (Cat# AAA161722)
Full Name
Active Annexin V (ANXA5)
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD40L-His at 10 ug/mL (100 uL/well) can bind Human CD40-Fc. The EC50 of Human CD40-Fc is 10-30 ng/mL.)
CD40, Active Protein (Cat# AAA257926)
Full Name
CD40 Protein, Human, Recombinant (ECD, hFc Tag)
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(Figure. The binding activity of GDNF with GFRa2.Glial cell-derived neurotrophic factor (GDNF) is a small protein that potently promotes the survival of many types of neurons. This protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Besides, Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 2 (GFRa2) has been identified as an interactor of GDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDNF and recombinant human GFRa2. Briefly, GDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GFRa2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-GDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GDNF and GFRa2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Regenerating Islet Derived Protein 3 Alpha, Active Protein (Cat# AAA150135)
Full Name
Active Regenerating Islet Derived Protein 3 Alpha (REG3a)
Gene Names
REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Afamin (AFM, also known as Alpha -Albumin), a bioactive protein, is expressed in the liver and secreted into the bloodstream. Human afamin is a 87 kDa protein which is encoded by the fourth member of the human albumin gene family. Mouse AFM shares 66% aa sequence identity with Full-length human AFM. This protein can bind and transport vitamin E across the blood-brain barrier in body fluids under conditions where the lipoprotein system is not sufficient, it also can act as a carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse AFM and recombinant human WNT3A. Briefly, AFM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to WNT3A-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AFM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse AFM and recombinant human WNT3A was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL.)
Afamin (AFM), Active Protein (Cat# AAA161881)
Full Name
Active Afamin (AFM)
Gene Names
Afm; Alf; alpha-Alb
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B)
B-Lymphocyte Activation Antigen B7-1 (LAB7-1), Active Protein (Cat# AAA153085)
Full Name
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Thyroid Stimulating Hormone, Active Protein (Cat# AAA11501)
Full Name
Thyroid Stimulating Hormone, High Purity
Purity
> 95% (SDS-PAGE)
Pricing
Application Data
(Figure. Cell proliferation of Raji cells after stimulated with IL6.Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant dog IL6. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL6 for 5 days. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of Raji cells.(A) Raji cells cultured in 1640, stimulated with 1ng/mL IL6 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.)
Interleukin 6, Active Protein (Cat# AAA150062)
Full Name
Active Interleukin 6 (IL6)
Gene Names
IL6; IL-6
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Application Data
TFF3, Active Protein (Cat# AAA214283)
Full Name
Recombinant Human TFF3
Gene Names
TFF3; ITF; P1B; TFI
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains)
Toll Like Receptor 2 (TLR2), Active Protein (Cat# AAA153053)
Full Name
Active Toll Like Receptor 2 (TLR2)
Gene Names
TLR2; TIL4; CD282
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure. The binding activity of PK2 with PKR1)
Prokineticin 2, Active Protein (Cat# AAA150071)
Full Name
Active Prokineticin 2 (PK2)
Gene Names
PROK2; BV8; HH4; PK2; KAL4; MIT1
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant chicken CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161775)
Full Name
Active Carbonic Anhydrase II (CA2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
Arginase (ARG), Active Protein (Cat# AAA148187)
Full Name
Active Arginase (ARG)
Reactivity
Rat
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
SDS-PAGE
(SDS-PAGE)
Acid Sphingomyelinase (ASM), Active Protein (Cat# AAA152332)
Full Name
Active Acid Sphingomyelinase (ASM)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
SDS_PAGE
(Sample: Active recombinant ESM1, Human)
Endothelial Cell Specific Molecule 1 (ESM1), Active Protein (Cat# AAA148202)
Full Name
Active Endothelial Cell Specific Molecule 1 (ESM1)
Gene Names
ESM1; endocan
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Bioactivity
(Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells.The ED50 for this effect is typically 0.54 ng/ml.)
Stem Cell Factor (SCF), Active Protein (Cat# AAA161692)
Full Name
Active Stem Cell Factor (SCF)
Gene Names
KITLG; CSF; MGF; 710-712
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Immobilized Recombinant SARS-CoV-2 Envelope at 2ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/mL.)
COVID 19 Envelope Coronavirus, Active Protein (Cat# AAA28309)
Full Name
Recombinant SARS-CoV-2 Envelope Protein
Purity
> 95% by SDS-PAGE.
Pricing
Bioactivity
(Aspartate transaminase(AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or glutamic oxaloacetic transaminase, is a pyridoxal phosphate-dependent transaminase enzyme AST catalyzes the reversible transfer of an alpha-amino group between aspa)
Aspartate Aminotransferase (AST), Active Protein (Cat# AAA153078)
Full Name
Active Aspartate Aminotransferase (AST)
Gene Names
Got1; cCAT; Got-1; cAspAT; AI789014
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Application Data
(Conditioned media from BMPC regulates miR-155 expression and fibrogenic response in mouse cardiac fibroblasts in vitro.Neutralizing antibodies against HGF (HGF-Ab) reversed this effect as compared to IgG treated cells.)
Hepatocyte Growth Factor, Active Protein (Cat# AAA76400)
Full Name
Mouse Hepatocyte Growth Factor Recombinant
Gene Names
Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
Purity
>97%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of sixIGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structuresand bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Withintissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and blocktheir access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.IGFBP3 also interacts with cell-surface proteins, affecting cell signaling fromoutside the cell or after internalization, and also enters the cell nucleus where itbinds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)has been identified as an interactor of IGFBP3, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human IGFBP3 andrecombinant human FN.Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100ul were then transferred to FN-coated microtiter wells andincubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1hwith anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78ng/ml.)
Insulin Like Growth Factor Binding Protein 3 (IGFBP3), Active Protein (Cat# AAA161673)
Full Name
Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
Gene Names
IGFBP3; IBP3; BP-53
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Neutrophil elastase (NE), also known as polymorphonuclear leukocyte elastase, is a major protease in the primary granules of neutrophils, is involved in microbicidal activity. It is located primarily in the azurophil granules of polymorphonuclear leukocytes. NE is an important factor promoting inflammation, has bactericidal effects, and shortens the inflammatory process. NE also regulates tumor growth by promoting metastasis and tumor microenvironment remodeling. However, NE plays a role in killing tumors under certain conditions and promotes other diseases such as pulmonary ventilation dysfunction. Additionally, it plays a complex role in various physiological processes and mediates several diseases. Myeloperoxidase (MPO) has been identified as an interactor of NE, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human NE and recombinant rat MPO. Briefly, biotin-linked NE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MPO-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human NE and recombinant rat MPO was shown in Figure 1, the EC50 for this effect is 0.66 ug/mL.)
Neutrophil Elastase (NE), Active Protein (Cat# AAA161712)
Full Name
Active Neutrophil Elastase (NE)
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Collagen Type I Alpha 2 (COL1a2) is a major component of collagen type I found in skin, bone, muscle, tendons, ligaments and other connective tissues.The COL1a2 protein is essential for the assembly, secretion, and stability of collagen fibers, which are vital for tissue development and repair.Mutations in COL1A2 are associated with a variety of genetic disorders, such as osteogenesis imperfecta (dysplasia of the bones) and cutis laxa. These diseases are often accompanied by weak bones and loss of skin elasticity. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of COL1a2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat COL1a2 and recombinant human FBN1. Briefly, COL1a2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FBN1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-COL1a2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat COL1a2 and recombinant human FBN1 was shown in Figure 1, the EC50 for this effect is 2.53 ug/mL.)
Collagen Type I Alpha 2 (COL1a2), Active Protein (Cat# AAA161716)
Full Name
Active Collagen Type I Alpha 2 (COL1a2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
DNase I, Active Protein (Cat# AAA258026)
Full Name
DNase I Protein, Human, Recombinant (His Tag)
Gene Names
DNASE1; DNL1; DRNI
Purity
>85% as determined by SDS-PAGE
Pricing
SDS-PAGE
Carboxypeptidase B1, Active Protein (Cat# AAA27761)
Full Name
Carboxypeptidase B1 Protein, Human, Recombinant (His Tag)
Gene Names
CPB1; CPB; PASP; PCPB
Purity
>98% as determined by SDS-PAGE
Pricing
Bioactivity
(Homing Associated Cell Adhesion Molecule (HCAM), also known as CD44, is a ubiquitous multistructural and multifunctional cells surface adhesion molecule involved in cell-cell and cell-matrix interactions. CD44 is broadly expressed, including in the membranes of B cells, granulocytes, monocytes, and erythrocytes as well as on many thymocytes and mature T cells, besides it is highly expressed in many cancers and regulates metastasis via recruitment of CD44 to the cell surface. This protein is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HCAM and biotinylated hyaluronan (HA). Briefly, biotin-linked HA was diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HCAM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 1 hour, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse HCAM and biotinylated HA was shown in Figure 1, and this effect was in a dose dependent manner.)
Homing Associated Cell Adhesion Molecule (HCAM), Active Protein (Cat# AAA161765)
Full Name
Active Homing Associated Cell Adhesion Molecule (HCAM)
Gene Names
Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
SDS-PAGE
AGO2 / Argonaute 2 / EIF2C2, Active Protein (Cat# AAA173551)
Full Name
Recombinant Human AGO2/Argonaute 2/EIF2C2 Protein (His Tag)(Active)
Gene Names
AGO2; PPD; Q10; CASC7; EIF2C2; LINC00980
Purity
>85 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
Leukocyte surface antigen CD47, Active Protein (Cat# AAA244029)
Full Name
Recombinant Human Leukocyte surface antigen CD47 (CD47), partial
Gene Names
CD47; IAP; OA3; MER6
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
WB (Western Blot)
(Western Blot Sample: Recombinant PIIINP, Human;Antibody: Rabbit Anti-Human PIIINP Ab)
Procollagen III N-Terminal Propeptide, Active Protein (Cat# AAA150095)
Full Name
Active Procollagen III N-Terminal Propeptide (PIIINP)
Gene Names
COL3A1; EDS4A; EDSVASC; PMGEDSV
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Pricing
Application Data
Interferon Gamma (IFNg), Active Protein (Cat# AAA146601)
Full Name
Active Interferon Gamma (IFNg)
Gene Names
IFNG; IFG; IFI
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(Thrombopoietin (TPO) is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis.TPO can promote platelet production, aggregation, ECM adhesion, and activation. It is principally produced in t)
Thrombopoietin (TPO), Active Protein (Cat# AAA152997)
Full Name
Active Thrombopoietin (TPO)
Gene Names
Thpo; Ml; Tpo; Mgdf; Tpo1; Tpo2; Tpo3; Tpo4; Mpllg
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
VEGF145, Active Protein (Cat# AAA14947)
Full Name
Human VEGF145
Gene Names
VEGFA; VPF; VEGF; MVCD1
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Purity
(The purity of LRRK2 (I2020T) was determined to be >70% by densitometry, approx. MW 210 kDa.)
LRRK2, Active Protein (Cat# AAA14255)
Full Name
LRRK2 Mutant (I2020T), Active
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Purity
>70%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.