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10000 results for "Recombinant Proteins" - showing 8600-8650
Bioactivity
(Interferon Beta (IFNb) is belongs to type I interferons (IFNs) family which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IFNb and recombinant human IFNa/bR1. Briefly, IFNb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNb and IFNa/bR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Beta (IFNb), Active Protein (Cat# AAA152231)
Full Name
Active Interferon Beta (IFNb)
Gene Names
Ifnb1; Ifb; IFNB; IFN-beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Recombinant Human MMP-7 cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RD#ES001). The specific activity is >812 pmol/min/ug, as measured under the described conditions.)
MMP-7, Recombinant Protein (Cat# AAA283372)
Full Name
Recombinant Human MMP-7 Protein
Purity
>90% by SDS-PAGE.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NCOA1 antibody. Blue: DAPI for nuclear staining.)
NCOA1, Polyclonal Antibody (Cat# AAA36958)
Full Name
NCOA1 Polyclonal Antibody
Gene Names
NCOA1; SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using EPB41 antibody. Blue: DAPI for nuclear staining.)
EPB41, Polyclonal Antibody (Cat# AAA36843)
Full Name
EPB41 Polyclonal Antibody
Gene Names
EPB41; HE; EL1; 4.1R
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody at dilution of 1:100 (40x lens).)
UBE2I, Polyclonal Antibody (Cat# AAA37049)
Full Name
UBE2I Polyclonal Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SOX17 protein (Right) or empty vector (Left) detected with SOX17 antibody)
SOX17, Monoclonal Antibody (Cat# AAA107093)
Full Name
SOX17 antibody
Applications
Western Blot
Purity
SOX17 antibody was purified by affinity chromatography.
Pricing
Amino Acid Info
(Amino Acid info)
Transglutaminase 6 (TGM6), Recombinant Protein (Cat# AAA153662)
Full Name
Recombinant Transglutaminase 6 (TGM6)
Gene Names
TGM6; TG6; TGY; SCA35; TGM3L; dJ734P14.3
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ST3GAL4 antibody. Blue: DAPI for nuclear staining.)
ST3GAL4, Polyclonal Antibody (Cat# AAA37606)
Full Name
ST3GAL4 Polyclonal Antibody
Gene Names
ST3GAL4; STZ; SAT3; CGS23; SIAT4; NANTA3; SIAT4C; ST3GalIV
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
Application Data
(HTRF Assay for Recombinant BRD1 (556-688) activity. 3.3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD1 (556-688) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
BRD1, Recombinant Protein (Cat# AAA59683)
Full Name
Recombinant BRD1 (556-688) protein
Gene Names
BRD1; BRL; BRPF1; BRPF2
Purity
The recombinant protein is >86% pure by SDS-PAGE.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using ABAT antibody. Blue: DAPI for nuclear staining.)
ABAT, Polyclonal Antibody (Cat# AAA37614)
Full Name
ABAT Polyclonal Antibody
Gene Names
ABAT; GABAT; NPD009; GABA-AT
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of PGD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
PGD, Monoclonal Antibody (Cat# AAA48230)
Full Name
PGD antibody
Gene Names
PGD; 6PGD
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IHC (Immunohistochemisry)
(Immunochemical staining of human FOLR2 in human spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
FOLR2, Polyclonal Antibody (Cat# AAA255362)
Full Name
Anti-FOLR2 Antibody, Rabbit Polyclonal
Gene Names
FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of Cofilin1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cofilin1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Cofilin 1, Monoclonal Antibody (Cat# AAA48042)
Full Name
Cofilin 1 antibody
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(Anti-SULT1A1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:35 kDa)
SULT1A1, Polyclonal Antibody (Cat# AAA255453)
Full Name
Anti-SULT1A1 Antibody, Rabbit Polyclonal
Gene Names
SULT1A1; PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-IL1F8 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:16 kDa(We are unsure as to the identity of these extra bands.))
IL1-F8, Polyclonal Antibody (Cat# AAA257254)
Full Name
Anti-IL1-F8 Antibody, Rabbit Polyclonal
Gene Names
Il1f8; Il36b; 2310043N20Rik
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(Mouse UCHL3 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse UCHL3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
UCHL3, Monoclonal Antibody (Cat# AAA257210)
Full Name
Recombinant Anti-UCHL3 Antibody, Rabbit Monoclonal
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
IHC (Immunohiostchemistry)
(Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human beta-Tubulin antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
TUBB2b, Monoclonal Antibody (Cat# AAA48070)
Full Name
TUBB2b antibody
Gene Names
TUBB2B; CDCBM7; PMGYSA; bA506K6.1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Anti- IP3 receptor antibody, AAA46090,IHC(P)IHC(P): Human Lung Cancer Tissue)
Inositol 1,4,5-trisphosphate receptor type 1, Polyclonal Antibody (Cat# AAA46090)
Full Name
Anti-IP3 receptor Antibody
Gene Names
ITPR1; ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(SUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-SUB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUB1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
PC4/SUB1, Polyclonal Antibody (Cat# AAA255757)
Full Name
Anti-PC4/SUB1 Antibody, Rabbit Polyclonal
Gene Names
SUB1; P15; PC4; p14
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant FHL1 protein using FHL1 antibody)
FHL1, Monoclonal Antibody (Cat# AAA106296)
Full Name
FHL1 antibody
Gene Names
FHL1; KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; SLIM-1; SLIMMER
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
FHL1 antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of SGTA in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SGTA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
SGTA, Monoclonal Antibody (Cat# AAA48219)
Full Name
SGTA antibody
Gene Names
SGTA; SGT; hSGT; alphaSGT
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of KRT23 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT23 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
KRT23, Monoclonal Antibody (Cat# AAA48122)
Full Name
KRT23 antibody
Gene Names
KRT23; K23; CK23; HAIK1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(Anti-PTPN2 rabbit polyclonal antibody at 1:500 dilutionLane A: MOLT4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:51 kDa(We are unsure as to the identity of these extra bands.))
TCPTP, Polyclonal Antibody (Cat# AAA255014)
Full Name
Anti-TCPTP Antibody, Rabbit Polyclonal
Gene Names
PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Western blot detection of hHR23b in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using hHR23b antibody (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.)
hHR23b, Monoclonal Antibody (Cat# AAA300769)
Full Name
hHR23b Monoclonal Antibody
Gene Names
RAD23B; P58; HR23B; HHR23B
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ARFGAP1 protein using ARFGAP1 antibody)
ARFGAP1, Monoclonal Antibody (Cat# AAA106452)
Full Name
ARFGAP1 antibody
Gene Names
ARFGAP1; ARF1GAP; HRIHFB2281
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
ARFGAP1 antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RASSF1 protein using RASSF1 antibody)
RASSF1, Monoclonal Antibody (Cat# AAA108222)
Full Name
RASSF1 antibody
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
RASSF1 antibody was purified by affinity chromatography.
Pricing
Application Data
(PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.)
PRMT6, Recombinant Protein (Cat# AAA59658)
Full Name
Recombinant PRMT6 protein
Gene Names
PRMT6; HRMT1L6
Purity
This recombinant protein is >= 85% pure by SDS-PAGE.
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mono-nucleosomes H3K9ac (EPL) Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K9ac (EPL) (Lane 2) were detected with H3K9ac antibody and H4 antibody , respectively. H3 was detected as loading control. Only Recombinant Mononucleosomes H3K9ac (EPL) can be detected by anti-H3K9ac antibody.)
Mononucleosomes H3K9ac, Recombinant Protein (Cat# AAA60123)
Full Name
Recombinant Mononucleosomes H3K9ac (EPL)
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
WB (Western Blot)
(Anti-SBDS rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:36 kDa)
SBDS, Polyclonal Antibody (Cat# AAA257269)
Full Name
Anti-SBDS Antibody, Rabbit Polyclonal
Gene Names
Sbds; SDS; CGI-97; AI836084; 4733401P19Rik
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(ERK2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-ERK2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ERK2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa)
ERK2, Polyclonal Antibody (Cat# AAA254188)
Full Name
Anti-ERK2 Antibody, Rabbit Polyclonal
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
SDS-PAGE
Oncomodulin (OCM), Recombinant Protein (Cat# AAA133991)
Full Name
Recombinant Oncomodulin (OCM)
Gene Names
OCM; OM; OCM1; ONCM
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 95%
Pricing
IP (Immunoprecipitation)
(CNPY2 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-CNPY2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CNPY2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: kDa)
CNPY2, Polyclonal Antibody (Cat# AAA255694)
Full Name
Anti-CNPY2 Antibody, Rabbit Polyclonal
Gene Names
CNPY2; MSAP; TMEM4; ZSIG9; HP10390
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SIRT5 protein using SIRT5 antibody)
SIRT5, Monoclonal Antibody (Cat# AAA106684)
Full Name
SIRT5 antibody
Gene Names
SIRT5; SIR2L5
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
SIRT5 antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Anti-LRPAP1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: U87MG Whole Cell LysateLane C: PC3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)
LRPAP1, Polyclonal Antibody (Cat# AAA257129)
Full Name
Anti-LRPAP1 Antibody, Rabbit Polyclonal
Gene Names
Lrpap1; RAP; HBP44; C77774; AA617339; AI790446; AU042172
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
ELISA
(Biotinylated Human IGF1R/CD221 Protein ELISA DataImmobilized Anti-IGF1R Antibody at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA.)
IGF1R/CD221, Recombinant Protein (Cat# AAA76148)
Full Name
Biotinylated Human IGF1R/CD221 Protein Recombinant
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
ELISA
(Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 Protein ELISA DataImmobilized Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 at 1?g/ml (100?l/Well) on the plate. Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.)
ROR1, Recombinant Protein (Cat# AAA76346)
Full Name
Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 Protein Recombinant
Gene Names
ROR1; NTRKR1; dJ537F10.1
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
SDS-PAGE
(SDS-PAGE)
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Recombinant Protein (Cat# AAA152076)
Full Name
Eukaryotic Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
TIMP1; EPA; EPO; HCI; CLGI; TIMP
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Cathepsin D is a lysosomal aspartic protease of the pepsin family. Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 118), a propeptide (residues 1964), and a mature chain (residues 65412). The mature chain can be processed further to the light (residues 65161) and heavy (residues 169412) chains. It is expressed in most cells and overexpressed in breast cancer cells. It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis. The activity of recombinant human CTSD is measured by its ability to cleave a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 0.1 M NaOAc, 0.2 M NaCl, pH 3.5. The rhCTSD is diluted to 20 ug/ml in assay buffer, then incubated at 37 degree C for 30min. The incubated rhCTSD is diluted to 5 ug/mL in assay buffer. Loading into a black well plate 50 uL of 5 ug/mL rhCTSD and start the reaction by adding 50 uL of 60 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhCTSD. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human CTSD is > 675 pmol/min/ug.)
Cathepsin D (CTSD), Active Protein (Cat# AAA161838)
Full Name
Active Cathepsin D (CTSD)
Gene Names
CTSD; CPSD; CLN10
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of GNS using anti-GNS antibody (AAA124609).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse lung tissue lysates,Lane 6: mouse kidney tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse spleen tissue lysates,Lane 9: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.)
GNS, Polyclonal Antibody (Cat# AAA124609)
Full Name
Anti-GNS Picoband antibody
Gene Names
GNS; G6S
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Pricing
DB (Dot Blot)
(Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)
Tet3, Recombinant Protein (Cat# AAA59672)
Full Name
Recombinant Tet3 (824-1795) protein
Gene Names
TET3; hCG_40738
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified CD9Mouse Monoclonal Antibody (CD9/1619). Confirmation of Integrity and Purity of Antibody.)
CD9 (TSPAN29), Monoclonal Antibody (Cat# AAA214577)
Full Name
CD9 (TSPAN29) (Motility-Related Protein-1)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of PP2C alpha in HeLa cells. The cell was stained with APP0401 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
PP2C alpha, Monoclonal Antibody (Cat# AAA47976)
Full Name
PP2C alpha antibody
Gene Names
PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(HMT assay for recombinant PRMT5 protein activity 0.25 ug of Histone H4 was incubated with 0 ug, 0.075 ug, 0.15 ug, and 0.3 ug PRMT5 protein in a 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. Reaction products were run on a 12.5% SDS-PAGE and detected by H4R3me2s antibody . PRMT5 only was used as negative control. Western Blot was used for activity detection.)
PRMT5, Recombinant Protein (Cat# AAA59657)
Full Name
Recombinant PRMT5 protein
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Pricing
WB (Western Blot)
(Anti-MGC29506 rabbit polyclonal antibody at 1:500 dilutionLane A: Molt4 Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))
MZB1/PERP1, Polyclonal Antibody (Cat# AAA255838)
Full Name
Anti-MZB1/PERP1 Antibody, Rabbit Polyclonal
Gene Names
MZB1; PACAP; pERp1; MEDA-7
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-METTL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
METTL1, Polyclonal Antibody (Cat# AAA255422)
Full Name
Anti-METTL1 Antibody, Rabbit Polyclonal
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
ELISA
(Biotinylated Human IL-6 R alpha/CD126 Protein ELISA DataImmobilized Human IL-6, No Tag at 2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.54?g/ml determined by ELISA.)
IL-6 R alpha/CD126, Recombinant Protein (Cat# AAA76215)
Full Name
Biotinylated Human IL-6 R alpha/CD126 Protein Recombinant
Gene Names
IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
ELISA
(Biotinylated Human PD-L1/B7-H1 Protein ELISA DataImmobilized Anti-PD-L1 Antibody, hFc Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 2.8ng/ml determined by ELISA.)
PD-L1/B7-H1, Recombinant Protein (Cat# AAA76231)
Full Name
Biotinylated Human PD-L1/B7-H1 Protein Recombinant
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Amino Acid Info
Eukaryotic Target Of The Antiproliferative Antibody 1 (TAPA1), Protein (Cat# AAA153199)
Full Name
Eukaryotic Target Of The Antiproliferative Antibody 1 (TAPA1)
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using SDHB Mouse Monoclonal Antibody (SDHB/2382). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
SDHB (Succinate Dehydrogenase B), Monoclonal Antibody (Cat# AAA215191)
Full Name
SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker)
Gene Names
SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
Reactivity
Human
Applications
Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)
GRP94/HSP90B1, Antibody (Cat# AAA215236)
Full Name
GRP94/HSP90B1 (Endoplasmic Reticulum Marker)
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
Applications
Immunohistochemistry, Flow Cytometry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing