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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
8299 results for "Phosphorylation" - showing 3250-3300
WB (Western Blot)
(Western blot analysis of extract from HT-29 cells using NF-kappaB p105/p50 (Ab-337) antibody (Lane 1 and 2).)
NFkappaB-p105/p50, Antibody (Cat# AAA111086)
Full Name
NFkappaB-p105/p50 (Ab-337) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using HtrA2(Ab-142) antibody. Western blot analysis of extracts from HeLa cells, using HtrA2(Ab-142) antibody.)
HtrA2, Antibody (Cat# AAA110571)
Full Name
HtrA2 (Ab-142) Antibody
Gene Names
HTRA2; OMI; MGCA8; PARK13; PRSS25
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1(Phospho-Ser232) Antibody.)
G3BP-1, Polyclonal Antibody (Cat# AAA301654)
Full Name
G3BP-1 (Phospho-Ser232) Antibody
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.)
IKK-alpha, Polyclonal Antibody (Cat# AAA316513)
Full Name
IKK-alpha (Phospho-Thr23) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The lane on the right is blocked with the phospho peptide.)
IL-8R beta/CDw128 beta, Polyclonal Antibody (Cat# AAA316406)
Full Name
IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody
Gene Names
CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
IHC (Immunohiostchemistry)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human testis tissue using Ephrin B (Ab-330) antibody.)
EFNB1/2, Antibody (Cat# AAA112210)
Full Name
EFNB1/2 (Ab-330) Antibody
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mnk1 (Phospho-Thr385) antibody. Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Phospho-Thr385) antibody.)
Mnk1, Antibody (Cat# AAA109174)
Full Name
Mnk1 (Phospho-Thr385) Antibody
Gene Names
MKNK1; MNK1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(Western blot analysis of extract from MDA-MB-435 cells untreated or treated with UV, using MKK6 (Ab-207) antibody and MKK6 (phospho-Ser207) antibody.)
MKK6, Antibody (Cat# AAA111203)
Full Name
MKK6 (Phospho-Ser207) Antibody
Gene Names
MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.)
IRF-3, Antibody (Cat# AAA110646)
Full Name
IRF-3 (Phospho-Ser385) Antibody
Gene Names
IRF3; IIAE7
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody (left)or the same antibody preincubated with blocking peptide (right).)
FER, Polyclonal Antibody (Cat# AAA243206)
Full Name
Phospho-FER (Tyr402) Antibody
Gene Names
FER; FerT; TYK3; PPP1R74
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by UV using 2.5ug Phospho-SMC1A-S957 antibody. Western blot was performed from the immunoprecipitate using Phospho-SMC1A-S957 antibody at a dilition of 1:1000.)
SMC1A-S957, Antibody (Cat# AAA36761)
Full Name
Phospho-SMC1A-S957 Polyclonal Antibody
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
Application Data
NFkB-p100, Polyclonal Antibody (Cat# AAA47800)
Full Name
NFkB-p100 (Phospho-Ser870)
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p16-INK4a (Phospho-Ser152) antibody. The picture on the right is treated with the synthesized peptide.)
p16-INK4a, Polyclonal Antibody (Cat# AAA306318)
Full Name
p16-INK4a (Phospho-Ser152) Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser190) antibody (left)or the same antibody preincubated with blocking peptide (right).)
FADD, Polyclonal Antibody (Cat# AAA305159)
Full Name
FADD (Phospho-Ser191) Antibody
Gene Names
FADD; GIG3; MORT1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells treated with EPO 20U/ml 15', using p16-INK4a (Phospho-Ser152) Antibody. The lane on the right is blocked with the phospho peptide.)
p16-INK4a, Polyclonal Antibody (Cat# AAA316657)
Full Name
p16-INK4a (Phospho-Ser152) Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
SDS-PAGE
Free Fatty Acid Receptor 2 (FFAR2), Recombinant Protein (Cat# AAA151067)
Full Name
Recombinant Free Fatty Acid Receptor 2 (FFAR2)
Reactivity
Rattus norvegicus (Rat)
Applications
Western Blot
Purity
> 90%
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using EPHB1/2 antibody)
EPHB1/2, Polyclonal Antibody (Cat# AAA108370)
Full Name
EPHB1/2 antibody
Gene Names
EPHB1; ELK; NET; Hek6; EPHT2
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCbeta (Phospho-Thr641) antibody)
PKC beta, Polyclonal Antibody (Cat# AAA107784)
Full Name
PKC beta antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PKC beta antibody was purified by immunoaffinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDK7 (Phospho-Thr170) antibody. Western blot analysis of extracts from HeLa cells, treated with Calyculin A (50nM, 30mins), using CDK7 (Phospho-Thr170) antibody.)
CDK7, Antibody (Cat# AAA111657)
Full Name
CDK7 (Phospho-Thr170) Antibody
Gene Names
CDK7; CAK; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
Application Data
(Sequence)
Transferrin Receptor 2 (TFR2), Recombinant Protein (Cat# AAA133956)
Full Name
Recombinant Transferrin Receptor 2 (TFR2)
Gene Names
TFR2; HFE3; TFRC2
Applications
Western Blot
Purity
>90%
Pricing
SDS-PAGE
DNA Repair Protein RAD50 (RAD50), Recombinant Protein (Cat# AAA134084)
Full Name
Recombinant DNA Repair Protein RAD50 (RAD50)
Gene Names
RAD50; NBSLD; RAD502; hRad50
Applications
Western Blot
Purity
> 97%
Pricing
SDS-PAGE
Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17), Recombinant Protein (Cat# AAA149278)
Full Name
Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17)
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 80%
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody. Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Neutrophil Cytosol Factor 1, Antibody (Cat# AAA112188)
Full Name
Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NFkappaB-p105/p50(Phospho-Ser337) Antibody.)
NFkappaB-p105/p50, Polyclonal Antibody (Cat# AAA306251)
Full Name
NFkappaB-p105/p50(Phospho-Ser337) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody.)
Histone H3.1, Polyclonal Antibody (Cat# AAA303320)
Full Name
Histone H3.1 (Phospho-Ser10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
SDS-PAGE
Caspase 11 (CASP11), Recombinant Protein (Cat# AAA135518)
Full Name
Recombinant Caspase 11 (CASP11)
Gene Names
Scaf11; SIP1; CASP11; SRRP129; Sfrs2ip; Srsf2ip; AI462454; mKIAA3013; 1110061H03Rik; 2610510E10Rik
Applications
Western Blot
Purity
> 97%
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Merlin (Phospho-Ser10) antibody. Western blot analysis of extracts from 3T3 cells, treated with IFN (2500U/ml, 30mins), using Merlin (Phospho-Ser10) antibody.)
Merlin, Antibody (Cat# AAA109181)
Full Name
Merlin (Phospho-Ser10) Antibody
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human tonsil tissue with Stat4 Polyclonal Antibody.)
Stat4, Polyclonal Antibody (Cat# AAA173768)
Full Name
Stat4 Polyclonal Antibody
Gene Names
STAT4; SLEB11
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using Phospho-GSK3 alpha/beta (Tyr279/216) Antibody)
GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA321641)
Full Name
Phospho-GSK3 alpha/beta (Tyr279/216) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using at 1: 2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain. )
BFAR, Polyclonal Antibody (Cat# AAA78312)
Full Name
Polyclonal Antibody to BFAR (BAR)
Gene Names
BFAR; BAR; RNF47
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Pricing
WB (Western Blot)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Cytochrome b-c1 complex subunit 10, Polyclonal Antibody (Cat# AAA81433)
Full Name
Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Applications
ELISA
Purity
>95%, Protein G purified
Pricing
SDS-PAGE
Nuclear Receptor Coactivator 3, Recombinant Protein (Cat# AAA144386)
Full Name
Recombinant Nuclear Receptor Coactivator 3 (NCOA3)
Gene Names
Ncoa3; Actr; Aib1; Rac3; Src3; pCip; Tram1; p/Cip; KAT13B; Tram-1; bHLHe42; AW321064; 2010305B15Rik
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 97%
Pricing
IHC (Immunohistochemisry)
(AAA322315 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT5A, Polyclonal Antibody (Cat# AAA322315)
Full Name
STAT5A Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(WB Suggested Anti-AKT1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)
AKT1, Polyclonal Antibody (Cat# AAA201690)
Full Name
AKT1 antibody - N-terminal region
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Cow, Dog, Goat, Human, Mouse, Pig, Rat, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of pancreas lysate using SNAI2 antibody. The lane on the left is treated with the antigen-specific peptide.)
SNAI2, Polyclonal Antibody (Cat# AAA325983)
Full Name
SNAI2 Antibody
Gene Names
SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Human A549 Whole Cell)
KDR, Polyclonal Antibody (Cat# AAA201730)
Full Name
KDR antibody - N-terminal region
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Horse, Human, Pig
Predicted Reactivity: Cow, Dog, Horse, Human, Pig
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(AAA322152 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
SNAI1, Polyclonal Antibody (Cat# AAA322152)
Full Name
SNAI1 Antibody
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA321564 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p105/p50, Polyclonal Antibody (Cat# AAA321564)
Full Name
Phospho-NF kappaB p105/p50 (Ser907) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
SDS-PAGE
(SDS-PAGE of ~14 kDa Active Human Recombinant Alpha Synuclein Protein Aggregate. Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.)
Alpha Synuclein, Active Protein (Cat# AAA253923)
Full Name
Alpha Synuclein Protein
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Applications
Western Blot
Purity
>95%
Ion-exchange Purified
Ion-exchange Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.)
STAT3, Monoclonal Antibody (Cat# AAA124520)
Full Name
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity-chromatography
Pricing
DB (Dot Blot)
(DotBlotanalysisofHuman,Mouse,RatTau2N4RP301S(phospho&non-phospho)showingdetectionofTauproteinusingMouseAnti-TauMonoclonalAntibody,Clone15B7 .Lane1:hTau2N4RP301SMonomer(0.5ug).Lane2:hTau2N4RP301SMonomer(1.0ug).Lane3:RecombinanthTau-441(2N4R)P301SMonomer,Baculovirus/Sf9Expressed(phosphorylated)(0.5ug).Lane4:RecombinanthTau-441(2N4R)P301SMonomer,Baculovirus/Sf9Expressed(phosphorylated)(1.0ug).Load:0.5ug,1.0ug.Block:5%BSAinTBST.PrimaryAntibody:MouseAnti-TauMonoclonalAntibody at1:4forOvernight,4C,withshaking.SecondaryAntibody:Goatanti-mouseIgG:HRPat1:4000for1houratRTwithshaking.ColorDevelopment:ChemiluminescentforHRP(Moss)for5mininRT.DotBlotperformedwithsupernatants.)
Tau (pThr217) IgG1 Kappa, Monoclonal Antibody (Cat# AAA254065)
Full Name
Mouse Anti-Human Tau (pThr217) Monoclonal IgG1 Kappa
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Dot Blot, Western Blot
Purity
Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
cGKI (cGKI beta), Polyclonal Antibody (Cat# AAA285984)
Full Name
cGKI (cGKI beta) Antibody (C-term)
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM/FACS (Flow Cytometry)
(CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CEBPB, Polyclonal Antibody (Cat# AAA285541)
Full Name
CEBPB Antibody (C-term)
Gene Names
CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
Interferon Gamma Receptor 2 (IFNgR2), Active Protein (Cat# AAA161936)
Full Name
Active Interferon Gamma Receptor 2 (IFNgR2)
Gene Names
IFNGR2; AF-1; IFGR2; IFNGT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Standard Curve (Sample)
Insulin Receptor Substrate 2 Phosphorylation (IRS2), ELISA Kit (Cat# AAA203690)
Full Name
Human Insulin Receptor Substrate 2 Phosphorylation (IRS2) ELISA Kit
Reactivity
Human
Pricing
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X)
Histone H2A.X, Monoclonal Antibody (Cat# AAA290507)
Full Name
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Pricing
WB (Western Blot)
(WB Suggested Anti-BCL2 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Placenta)
BCL2, Polyclonal Antibody (Cat# AAA201655)
Full Name
BCL2 antibody - N-terminal region
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western Blot analysis of HuvEc cells using MerTK/Tyro3 Polyclonal Antibody)
MERTK/TYRO3, Polyclonal Antibody (Cat# AAA236302)
Full Name
MERTK/TYRO3 Antibody
Gene Names
MERTK; MER; RP38; c-mer
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western Blot analysis of RAT-KIDNEY cells using Phospho-GSK3beta (S9) Polyclonal Antibody)
GSK3B, Polyclonal Antibody (Cat# AAA235827)
Full Name
Phospho-GSK3B (S9) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(WB Suggested Anti-Irf1 Antibody Titration: 0.2-1 ug/mlPositive Control: Mouse Muscle)
Irf1, Polyclonal Antibody (Cat# AAA198145)
Full Name
Irf1 antibody - C-terminal region
Gene Names
Irf1; Irf-1; AU020929
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep
Applications
Western Blot
Purity
Affinity Purified
Pricing