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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A549 cells using anti-BAP29/BCAP29 antibody (AAA19965).Overlay histogram showing A549 cells stained with AAA19965 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP29/BCAP29 Antibody (AAA19965, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of BAP29/BCAP29 using anti-BAP29/BCAP29 antibody (AAA19965).BAP29/BCAP29 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP29/BCAP29 Antibody (AAA19965) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP29/BCAP29 Antibody (AAA19965) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP29/BCAP29 Antibody (AAA19965) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP29/BCAP29 Antibody (AAA19965) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP29/BCAP29 Antibody (AAA19965) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U251 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP29/BCAP29 antigen affinity purified polyclonal antibody (#AAA19965) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAP29/BCAP29 at approximately 25 kDa. The expected band size for BAP29/BCAP29 is at 28 kDa.)

BAP29/BCAP29, Polyclonal Antibody (Cat# AAA19965)

Full Name

Anti-BAP29/BCAP29 Antibody Picoband

Gene Names

BCAP29; BAP29

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

Autophagy protein 5, ATG5, ELISA Kit (Cat# AAA22525)

Full Name

Human Autophagy protein 5, ATG5 ELISA Kit

Gene Names

ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE

Reactivity

Human

Pricing

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

PIP5K3, Monoclonal Antibody (Cat# AAA24317)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

MLH1, Monoclonal Antibody (Cat# AAA24573)

Full Name

MLH1 (MutL Homolog 1, MutL Protein Homolog 1, COCA2, DNA Mismatch Repair Protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, MGC5172, MutL Homolog 1 Colon Cancer Nonpolyposis Type 2) APC

Gene Names

MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

EHD3, Monoclonal Antibody (Cat# AAA25085)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) (FITC)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in PC-12 (Cat # L012V1).)

USF2, Monoclonal Antibody (Cat# AAA26109)

Full Name

USF2 (Upstream Transcription Factor 2, c-fos Interacting, FIP, bHLHb12) (APC)

Gene Names

USF2; FIP; bHLHb12

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

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WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).)

CDK4, Monoclonal Antibody (Cat# AAA26365)

Full Name

CDK4 (Cyclin-Dependent Kinase 4, CMM3, MGC14458, PSK-J3) (FITC)

Gene Names

CDK4; CMM3; PSK-J3

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

ASB3, Polyclonal Antibody (Cat# AAA22266)

Full Name

ASB3 Polyclonal Antibody

Gene Names

ASB3; ASB-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-PDIA6 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HepG2 cell lysate.PDIA6 is strongly supported by BioGPS gene expression data to be expressed in HepG2)

PDIA6, Polyclonal Antibody (Cat# AAA23546)

Full Name

PDIA6 antibody - middle region

Gene Names

PDIA6; P5; ERP5; TXNDC7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Jurkat, HDLM-2, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

LAG3, Monoclonal Recombinant Antibody (Cat# AAA23802)

Full Name

Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F]

Gene Names

LAG3; CD223

Reactivity

Human

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

PIP5K3, Monoclonal Antibody (Cat# AAA24058)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)

TADA2B, Monoclonal Antibody (Cat# AAA24570)

Full Name

TADA2B (Transcriptional Adapter 2-beta, ADA2-like Protein beta, ADA2-beta, ADA2B, MGC21874) APC

Gene Names

TADA2B; ADA2B; ADA2(beta)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

DUSP6, Monoclonal Antibody (Cat# AAA25082)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (FITC)

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

CD83 antigen (CD83), ELISA Kit (Cat# AAA27130)

Full Name

Bovine CD83 antigen (CD83) ELISA Kit

Gene Names

CD83; BL11; HB15

Reactivity

Bovine

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

NRF2, Polyclonal Antibody (Cat# AAA28410)

Full Name

NRF2 Rabbit pAb

Gene Names

LOC4339539; GPB-LR; RACK1B; OsJ_19467

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using PTPN6 antibody. Blue: DAPI for nuclear staining.)

PTPN6, Polyclonal Antibody (Cat# AAA29690)

Full Name

PTPN6 Antibody

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

Hepatocyte Growth Factor (HGF), ELISA Kit (Cat# AAA12795)

Full Name

Human Hepatocyte Growth Factor (HGF) ELISA kit

Reactivity

Human

Pricing

Progesterone, ELISA Kit (Cat# AAA14587)

Full Name

Progesterone ELISA Kit

Reactivity

Human

Pricing

Melatonin, ELISA Kit (Cat# AAA16891)

Full Name

Canine Melatonin ELISA Kit

Reactivity

Canine

Pricing

Recombinant Human Glycophorin-A

Gene Names

GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

CD133, Polyclonal Antibody (Cat# AAA29951)

Full Name

CD133 Antibody

Gene Names

PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

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ICC (Immunocytochemistry) (ICC staining STAT5b in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

STAT5b, Monoclonal Antibody (Cat# AAA30207)

Full Name

STAT5b Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

BCMA, Monoclonal Antibody (Cat# AAA30719)

Full Name

Anti-BCMA antibody (DM6), Rabbit mAb

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

Protein Kinase C alpha Type (PRKCA), ELISA Kit (Cat# AAA31487)

Full Name

Human Protein Kinase C alpha Type (PRKCA) ELISA Kit

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha

Reactivity

Human

Pricing

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647)

HLA ABC, Monoclonal Antibody (Cat# AAA11895)

Full Name

MOUSE ANTI HUMAN HLA ABC:HRP

Reactivity

Macaque, Bovine, Cynomolgus Monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew

Applications

Immunohistology Frozen*

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Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)

CD169, Monoclonal Antibody (Cat# AAA12223)

Full Name

RAT ANTI MOUSE CD169:Low Endotoxin

Gene Names

Siglec1; Sn; Cd169; Siglec-1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

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CD68, Monoclonal Antibody (Cat# AAA12147)

Full Name

MOUSE ANTI RAT CD68:Biotin

Applications

Flow cytometry (FC/FACS)*

Pricing

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CD204, Monoclonal Antibody (Cat# AAA12075)

Full Name

RAT ANTI MOUSE CD204:Low Endotoxin

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

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CD81, Monoclonal Antibody (Cat# AAA11941)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Reactivity

Rat

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

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Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)