Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 600-650 of 2583 product results
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150563)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of woundsIt can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Ra GAL7 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL7 is 25 ug/mL.)
Galectin 7 (GAL7), Active Protein (Cat# AAA152173)
Full Name
Active Galectin 7 (GAL7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains)
Toll Like Receptor 2 (TLR2), Active Protein (Cat# AAA153053)
Full Name
Active Toll Like Receptor 2 (TLR2)
Gene Names
TLR2; TIL4; CD282
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Epithelial Neutrophil Activating Peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines. Full-length ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. ENA-78 is expressed by many immune cells, such as macrop)
Epithelial Neutrophil Activating Peptide 78 (ENA78), Active Protein (Cat# AAA153064)
Full Name
Active Epithelial Neutrophil Activating Peptide 78 (ENA78)
Gene Names
Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 (assay buffer), initiated by addition 50 ul of 3 ug/ml ACP1 (diluted by assay buffer) to 50 uL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 ul of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/ug.)
Acid Phosphatase 1 (ACP1), Active Protein (Cat# AAA161821)
Full Name
Active Acid Phosphatase 1 (ACP1)
Gene Names
Acp1; Acp-1; LMW-PTP; AI427468; 4632432E04Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation. It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The activity of recombinant human TFPI was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 20 ul different concentrations of recombinant human TFPI (MW: 61.9 KD) was incubated with 20 ul diluted trypsin at 37 degree C for 15 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 20 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TFPI significantly decreased trypsin activity. The inhibition IC50 was )
Tissue Factor Pathway Inhibitor (TFPI), Active Protein (Cat# AAA161730)
Full Name
Active Tissue Factor Pathway Inhibitor (TFPI)
Gene Names
TFPI; EPI; TFI; LACI; TFPI1
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152987)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
LITAF; TNF-alpha
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
CYR61, Active Protein (Cat# AAA75577)
Full Name
CYR61 protein
Gene Names
CYR61; CCN1; GIG1; IGFBP10
Purity
> 95% pure
Pricing
MMP1, Active Protein (Cat# AAA75571)
Full Name
MMP1 protein
Gene Names
Mmp-1; Mmp1
Purity
> 90% pure
Pricing
Granzyme A, Active Protein (Cat# AAA77033)
Full Name
Granzyme A (human), (recombinant)
Gene Names
GZMA; HFSP; CTLA3
Purity
>98% (SDS-PAGE)
Pricing
Application Data
(Fig 2 Immobilized Recombinant Human ACE2 at 2 ugmL (100uLwell) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 ugmL.)
COVID 19 Spike S1 Coronavirus, Active Protein (Cat# AAA78534)
Full Name
Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)
Applications
Functional Assay
Purity
>90% by SDS-PAGE.
Pricing
Application Data
(TcREG inhibit osteoclast activity in an antigen- and contact-independent manner by secreted cytokines.Cells were re-fed with medium containing M-CSF and RANKL every 2 to 3 days.)
Macrophage Colony stimulating Factor, Active Protein (Cat# AAA76388)
Full Name
Mouse Macrophage Colony stimulating Factor Recombinant
Gene Names
Csf1; op; Csfm; MCSF; C87615
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
(Validation of the array expression pattern by quantitative RT-PCR.The mRNA levels of CCL2/MCP-1 , CCL5/RANTES , ICAM-1 , VCAM-1 , E-selectin , and P-selectin were determined by quantitative real-time PCR, and values were normalized to 18S rRNA as reference gene.)
RANTES-CCL5, Active Protein (Cat# AAA76394)
Full Name
Mouse RANTES-CCL5 Recombinant
Gene Names
Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
(Expression and function of the CXCR4 receptor in MET-depleted cell.(b) Western blot analysis of AKT and MAPK activation in shMET RH30 cells showed a decrease in the phosphorylation of these kinases following stimulation with human HGF (20?ng/ml) and SDF-1 (10?ng/ml and 100?ng/ml).)
SDF-1 alpha, Active Protein (Cat# AAA76395)
Full Name
Mouse SDF-1 alpha Recombinant
Gene Names
Cxcl12; Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
VEGF145, Active Protein (Cat# AAA79177)
Full Name
Human VEGF145
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
MIG (CXCL9), Active Protein (Cat# AAA79183)
Full Name
Human MIG (CXCL9)
Gene Names
CXCL9; CMK; MIG; Humig; SCYB9; crg-10
Reactivity
Human
Purity
>= 98% by SDS-PAGE gel and HPLC analyses.
Pricing
Application Data
IL-3, Active Protein (Cat# AAA79188)
Full Name
Human IL-3
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
GM-CSF, Active Protein (Cat# AAA79195)
Full Name
Human GM-CSF
Gene Names
CSF2; GMCSF
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
GM-CSF, Active Protein (Cat# AAA79202)
Full Name
Human GM-CSF
Gene Names
CSF2; GMCSF
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
IL-17A, Active Protein (Cat# AAA79205)
Full Name
Human IL-17A
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analyses
Pricing
Application Data
IL-6, Active Protein (Cat# AAA79207)
Full Name
Human IL-6
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
RANKL, soluble, Active Protein (Cat# AAA79211)
Full Name
Rat RANKL, soluble
Gene Names
Tnfsf11; RANKL
Reactivity
Rat
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
Application Data
IL-1 beta, Active Protein (Cat# AAA79215)
Full Name
Human IL-1 beta
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
BMP-13 (GDF6), Active Protein (Cat# AAA79222)
Full Name
Human BMP-13 (GDF6)
Gene Names
Gdf6; BMP13; GDF16
Reactivity
Human
Purity
> 95% by SDS-PAGE & HPLC analysis
Pricing
Application Data
SCF, Active Protein (Cat# AAA79223)
Full Name
Human SCF
Gene Names
KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
VEGF-E, Active Protein (Cat# AAA79244)
Full Name
Orf Virus VEGF-E
Reactivity
Orf virus
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Application Data
PDGF-AA, Active Protein (Cat# AAA79247)
Full Name
Human PDGF-AA
Gene Names
PDGFA; PDGF1; PDGF-A
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
GM-CSF, Active Protein (Cat# AAA79248)
Full Name
Human GM-CSF
Gene Names
CSF2; GMCSF
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
CD105/Endoglin, soluble, Active Protein (Cat# AAA79282)
Full Name
Mouse CD105/Endoglin, soluble
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Application Data
SCF, Active Protein (Cat# AAA79283)
Full Name
Human SCF
Gene Names
KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
VEGF-C, Active Protein (Cat# AAA79305)
Full Name
Human VEGF-C
Gene Names
VEGFC; VRP; Flt4-L
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
PlGF, Active Protein (Cat# AAA79311)
Full Name
Mouse PlGF
Gene Names
Pgf; PIGF; Plgf; AI854365
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
VEGF164, Active Protein (Cat# AAA79312)
Full Name
Mouse VEGF164
Gene Names
Vegfa; Vpf; Vegf
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
VEGF-F (Bothrops insularis), Active Protein (Cat# AAA79314)
Full Name
Snake VEGF-F (Bothrops insularis)
Reactivity
Snake
Purity
> 95% by SDS-PAGE & silver stain
Pricing
VEGFR-1/Flt-1(D7)-Fc Chimera, soluble, Active Protein (Cat# AAA79121)
Full Name
Human VEGFR-1/Flt-1(D7)-Fc Chimera, soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Isocitrate Dehydrogenase (NADP+), Active Protein (Cat# AAA78966)
Full Name
Yeast Isocitrate Dehydrogenase (NADP+) (rICDH (NADP))
Pricing
Application Data
D-Lactate dehydrogenase, Active Protein (Cat# AAA78979)
Full Name
D-Lactate dehydrogenase, recombinant from bacteria
Pricing
Bioactivity
SARS-CoV S1 Protein, Active Protein (Cat# AAA177981)
Full Name
Recombinant SARS-CoV S1 Protein (His Tag)(Active)
Purity
>90% as determined by SDS-PAGE
Pricing
SDS-PAGE
IL-12 p35, Active Protein (Cat# AAA178004)
Full Name
Recombinant Mouse IL-12 p35 protein (N-His)(active)
Purity
>98% as determined by SDS-PAGE.
Pricing
SDS-PAGE
COVID 19 Spike S1 Protein Coronavirus, Active Protein (Cat# AAA176996)
Full Name
Recombinant 2019-nCoV S1 Protein (mFc Tag)
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
CALMODULIN, Active Protein (Cat# AAA50491)
Full Name
NATIVE BOVINE CALMODULIN
Applications
ELISA
Purity
> 95%. Determined by SDS-PAGE.
Pricing
ELASTASE, Active Protein (Cat# AAA50496)
Full Name
NATIVE HUMAN ELASTASE (NEUTROPHIL)
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Applications
Functional Assay
Purity
>95% by SDS PAGE
Pricing
EGF, Active Protein (Cat# AAA50531)
Full Name
RECOMBINANT HUMAN EGF
Gene Names
EGF; URG; HOMG4
Applications
Western Blot, Functional Assay
Purity
>98% by SDS PAGE and HPLC analysis
Pricing
Bioactivity
(HTRF assay for RPS6KA1/RSK1 activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA1/RSK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
RPS6KA1/RSK1, Active Protein (Cat# AAA60316)
Full Name
Recombinant RPS6KA1/RSK1 protein
Purity
>=90%
Pricing
Bioactivity
(HTRF Assay for Recombinant PHKG2 activity. 1 uM STK S1 substrate was incubated with different concentrations of PHKG2 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Bioactivity
(HTRF Assay for Recombinant BRD2 (BD1+BD2) activity. 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 (BD1+BD2) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-His antibody was used to detect reaction products.)
BRD2 (BD1+BD2), Active Protein (Cat# AAA60342)
Full Name
Recombinant BRD2 (BD1+BD2) protein
Purity
>=85%
Pricing
Application Data
Growth Differentiation Factor 15 (hGDF15), Active Protein (Cat# AAA60567)
Full Name
Recombinant Human Growth Differentiation Factor 15 (hGDF15)
Purity
>95%, determined by SDS-PAGE
Pricing
IGF-I, Active Protein (Cat# AAA60570)
Full Name
Fish (denis) IGF-I
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Purity
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
super leptin antagonist, Active Protein (Cat# AAA60573)
Full Name
Rat super leptin antagonist (mutant D23/L39A/D40A/F41A)
Purity
The purity of super rat leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.