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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 600-650 of 2875 product results
Application Data
FGFR2, Active Protein (Cat# AAA258055)
Full Name
FGFR2 Protein, Human, Recombinant (ECD, His Tag)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 ug/ml (100 ul/well) can bind zebrafish EFNB2A-Fc, The EC50 of zebrafish EFNB2A-Fc is 12.8-30.1 ng/ml.)
EFNB2A, Active Protein (Cat# AAA258248)
Full Name
EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc Tag)
Gene Names
efnb2a; efnb2; ephrin-B2a; id:ibd5072
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA148178)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Application Data
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), Active Protein (Cat# AAA148219)
Full Name
Active Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Gene Names
Wnt5a; Wnt-5a; 8030457G12Rik
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells.Thus, chemotaxis assay used 24-well microchemotaxis syste)
Interleukin 8 (IL8), Active Protein (Cat# AAA152972)
Full Name
Active Interleukin 8 (IL8)
Gene Names
IL8; CXCL8; AMCF-I
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Hepatocyte Growth Factor, Active Protein (Cat# AAA38133)
Full Name
Recombinant Human Hepatocyte Growth Factor, CHO
Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Pyrococcus Fruriosus Deoxyuridine Triphosphatase, Active Protein (Cat# AAA38252)
Full Name
Recombinant Pyrococcus Fruriosus Deoxyuridine Triphosphatase
Purity
Greater than 97.0% as determined by SDS-PAGE.
Pricing
Leptin, Active Protein (Cat# AAA38172)
Full Name
Recombinant Mouse Leptin
Gene Names
Lep; ob; obese
Purity
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Pufferfish Leptin, Active Protein (Cat# AAA38733)
Full Name
Recombinant Pufferfish Leptin
Purity
Greater than 99.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing
FSH beta, Active Protein (Cat# AAA75383)
Full Name
FSH beta protein
Purity
> 98% by SDS-PAGE
Pricing
IGF1, Active Protein (Cat# AAA75399)
Full Name
IGF1 protein
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Purity
> 98% pure
Pricing
Sonic Hedgehog, Active Protein (Cat# AAA75584)
Full Name
Sonic Hedgehog protein
Gene Names
Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
Purity
> 98% pure
Pricing
Bacterial Protocatechuate 3,4-Dioxygenase (rPCO), Active Protein (Cat# AAA78973)
Full Name
Bacterial Protocatechuate 3,4-Dioxygenase (rPCO)
Pricing
Fc gamma RIIA/CD32a (R167H), Active Protein (Cat# AAA48984)
Full Name
Recombinant human Fc gamma RIIA/CD32a (R167H) protein
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Luteinizing Hormone, Active Protein (Cat# AAA44840)
Full Name
Luteinizing Hormone (LH), Human Pituitary Gland
Purity
>= 98% (SDS-PAGE)
Bioactivity
(The cytochrome P450 enzyme CYP2E1 catalyzes the oxidative metabolism of many solvents and other small organic molecules. CYP2E1 is expressed in adult and fetal human liver in addition to extrahepatic tissues such as lung and placenta. Treatment of primary cultures of human hepatocytes with ethanol induces CYP2E1 protein, and this is consistent with the finding that hepatic CYP2E1 protein and mRNA levels are increased in individuals with alcoholism. Although only a few drugs (e.g., acetaminophen have been identified as substrates for CYP2E1, many low molecular weight procarcinogens are activated by this cytochrome P450 (P450). Chlorzoxazone 6-hydroxylation, N-nitrosodimethylamine N-demethylation and p-nitrophenol hydroxylation can be used to measure the catalytic activity of CYP2E1. Thus, the recombinant mouse CYP2E1 activity was measured by its ability to hydroxylate p-nitrophenol to p-nitrocatechol. The reaction was performed in 50 mM potassium phosphate, pH 7.4 (Assay Buffer), initiated by addition 20 uL of 500 ug/ml CYP2E1 to 10 uL of 5 mM substrate p-nitrophenol and 30 ul of 26 mM NADPH in a total volume of 500 ul. Incubated at 37 degree C for 30min, then read at a wavelength of 535 nm after acidification of the reaction mixture with trichloroacetic acid followed by neutralization using 2 M NaOH.)
Cytochrome P450 2E1 (CYP2E1), Active Protein (Cat# AAA161818)
Full Name
Active Cytochrome P450 2E1 (CYP2E1)
Gene Names
Cyp2e1; Cyp2e
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Serum Amyloid A (SAA) is a class of 104 amino acid conservative acute-phase proteins, which is essential in immune-mediated inflammatory processes, like IBD. SAA has dual roles in the inflammatory process, which it can stimulates the pro-inflammatory cytokine expression and promotes the pathogenic differentiation of TH17 cells. In addition, SAA can remove toxic lipids produced during inflammatory responses and membrane debris from dead cells, redirect HDL, and recycle cholesterol for tissue repair. Besides, C Reactive Protein (CRP) has been identified as an interactor of SAA, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SAA and recombinant human CRP. Briefly, SAA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CRP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SAA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SAA and recombinant human CRP was shown in Figure 1, the EC50 for this effect is 5.3 ug/mL.)
Serum Amyloid A (SAA), Active Protein (Cat# AAA161801)
Full Name
Active Serum Amyloid A (SAA)
Gene Names
SAA1; SAA; PIG4; SAA2; TP53I4
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
IL2, Active Protein (Cat# AAA214316)
Full Name
Recombinant Mouse IL2
Gene Names
Il2; Il-2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.)
Erythropoietin, Active Protein (Cat# AAA258227)
Full Name
Erythropoietin Protein, Mouse, Recombinant (His Tag)
Purity
>96% as determined by SDS-PAGE
Pricing
Sox2, Active Protein (Cat# AAA79199)
Full Name
Human Sox2
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Purity
>95 % (SDS-PAGE, HPLC)
Pricing
Chemerin, Active Protein (Cat# AAA79234)
Full Name
Human Chemerin
Gene Names
RARRES2; TIG2; HP10433
Reactivity
Human
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
Complement Factor B, Active Protein (Cat# AAA75246)
Full Name
Complement Factor B
Gene Names
CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
Purity
> 95% by SDS-PAGE
Pricing
BAFF R/TNFRSF13C, Active Protein (Cat# AAA48988)
Full Name
Recombinant human BAFF R/TNFRSF13C protein
Gene Names
TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
PIGF-2, Active Protein (Cat# AAA48996)
Full Name
Recombinant human PIGF-2 protein
Gene Names
PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Vascular Endothelial Growth Factor (rHuVEGF), Active Protein (Cat# AAA47955)
Full Name
Recombinant Human Vascular Endothelial Growth Factor
Gene Names
VEGFA; VPF; VEGF; MVCD1; MGC70609
Purity
Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Purification: Chromatographic
Purification: Chromatographic
Pricing
Pegylated Granulocyte-Colony, Active Protein (Cat# AAA38985)
Full Name
Recombinant Human Pegylated Granulocyte-Colony Stimulating Factor
Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Purity
Greater than 95.0% as determined by SEC-HPLC.
Pricing
Follistatin, Active Protein (Cat# AAA38122)
Full Name
Recombinant Human Follistatin
Gene Names
FST; FS
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Tumor Necrosis Factor Receptor 2 Fusion Protein, Active Protein (Cat# AAA38200)
Full Name
Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE.
Pricing
Activin receptor type-2B (ACVR2B), Active Protein (Cat# AAA116934)
Full Name
Recombinant Human Activin receptor type-2B (ACVR2B), partial
Gene Names
ACVR2B; HTX4; ACTRIIB; ActR-IIB
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Tissue Inhibitors of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. The activity of recombinant mouse TIMP4 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rmTIMP4 (MW: 22.55 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rmTIMP4 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant mouse TIMP4 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), Active Protein (Cat# AAA161702)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)
Gene Names
Timp4; TIMP-4
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
EGF, Active Protein (Cat# AAA214337)
Full Name
Recombinant Rat EGF
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with IFN-.)
Interferon Alpha, Active Protein (Cat# AAA150049)
Full Name
Active Interferon Alpha (IFNa)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Interleukin-3, Active Protein (Cat# AAA38225)
Full Name
Recombinant Human Interleukin-3, His Tag
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Pricing
r-3alpha-Hydroxysteroid Dehydrogenase, Active Protein (Cat# AAA78965)
Full Name
r-3alpha-Hydroxysteroid Dehydrogenase (r-3alpha-HSDH (Microorganism)
Pricing
Fc epsilon RI alpha, Active Protein (Cat# AAA48987)
Full Name
Recombinant Human Fc epsilon RI alpha protein
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
SOD1, Active Protein (Cat# AAA48526)
Full Name
SOD1, 1-154aa, Human, E Coli (Bioactivity Validated)
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Application Data
SCF, Active Protein (Cat# AAA79223)
Full Name
Human SCF
Gene Names
KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
GRO-alpha, Active Protein (Cat# AAA38089)
Full Name
Recombinant Human GRO-alpha (CXCL1)
Gene Names
CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Vascular Endothelial Growth Inhibitor, Active Protein (Cat# AAA38546)
Full Name
Recombinant Human Vascular Endothelial Growth Inhibitor
Gene Names
TNFSF15; TL1; TL1A; VEGI; VEGI192A
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Progranulin, Active Protein (Cat# AAA38550)
Full Name
Recombinant Human Progranulin
Gene Names
GRN; GEP; GP88; PEPI; PGRN; CLN11; PCDGF
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Parathyroid Hormone, Active Protein (Cat# AAA38891)
Full Name
Recombinant Human Parathyroid Hormone (1-84) N15 Labeled
Gene Names
PTH; PTH1
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Fibroblast Growth Factor-8, Active Protein (Cat# AAA38020)
Full Name
Recombinant Mouse Fibroblast Growth Factor-8, 194 a.a.
Gene Names
Fgf8; Aigf; Fgf-8
Purity
Greater than 97.0% as determined by analysis by SDS-PAGE.
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His (40592-V08H80), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His (40592-V08H80) is 5-20 ng/mL.)
COVID 19 Spike RBD(Y453F)-His Coronavirus, Active Protein (Cat# AAA258101)
Full Name
SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein
Purity
>95% as determined by SDS-PAGE
Pricing
Caspase-5, Active Protein (Cat# AAA77045)
Full Name
Caspase-5 (human), (recombinant)
Gene Names
CASP5; ICH-3; ICEREL-III; ICE(rel)III
Purity
>80% (SDS-PAGE)
Pricing
Bioactivity
(Interferon Beta (IFNb) is belongs to type I interferons (IFNs) family which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IFNb and recombinant human IFNa/bR1. Briefly, IFNb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNb and IFNa/bR1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Beta (IFNb), Active Protein (Cat# AAA152231)
Full Name
Active Interferon Beta (IFNb)
Gene Names
Ifnb1; Ifb; IFNB; IFN-beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Interferon-alpha 2c, Active Protein (Cat# AAA38061)
Full Name
Recombinant Human Interferon-alpha 2c
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Purity
>97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
Growth and Differentiation factor 5, Active Protein (Cat# AAA38207)
Full Name
Recombinant Human Growth and Differentiation factor 5
Gene Names
GDF5; OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.