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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 650-700 of 2875 product results
Trefoil Factor-1, Active Protein (Cat# AAA38601)
Full Name
Recombinant Human Trefoil Factor-1
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Thyroid Stimulating Hormone, Active Protein (Cat# AAA39027)
Full Name
Human Thyroid Stimulating Hormone
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
Growth and Differentiation factor 5, Active Protein (Cat# AAA38207)
Full Name
Recombinant Human Growth and Differentiation factor 5
Gene Names
GDF5; OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Resistin, Active Protein (Cat# AAA38215)
Full Name
Recombinant Human Resistin
Gene Names
RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
Purity
Greater than 98% as determined by SDS-PAGE.
Pricing
Interferon-alpha 2c, Active Protein (Cat# AAA38061)
Full Name
Recombinant Human Interferon-alpha 2c
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Purity
>97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
EphA3, Active Protein (Cat# AAA48999)
Full Name
Recombinant mouse EphA3 protein
Gene Names
Epha3; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
NP-1, Active Protein (Cat# AAA79176)
Full Name
Human NP-1
Gene Names
NPTX1; NP1
Reactivity
Human
Purity
>98 % by SDS-PAGE, HPLC analysis
Pricing
MIA (Melanoma inhibitory activity), Active Protein (Cat# AAA79186)
Full Name
Human MIA (Melanoma inhibitory activity)
Gene Names
MIA; CD-RAP
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analysis
Pricing
FGFR-2/Fc Chimera, soluble, Active Protein (Cat# AAA79217)
Full Name
Human FGFR-2/Fc Chimera, soluble
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
IL-3 receptor (CD123), Active Protein (Cat# AAA76117)
Full Name
Human IL-3 receptor Recombinant (CD123)
Purity
>97%, as determined by SDS-PAGE and HPLC
Pricing
Insulin, Active Protein (Cat# AAA75245)
Full Name
Insulin protein
Gene Names
INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
Purity
> 99% pure
Pricing
Bioactivity
(Glypican-3 (GPC3), a 70 kDa protein, is a member of the glypican family that attaches to the cell surface by a glycosylphosphatidylinositol anchor, is specifically up-regulated in hepatocellular carcinoma (HCC) although rarely or not expressed in normal liver tissues, making it a perfect test and treatment target for HCC. GPC3 is also a negative transcriptional regulator and tumor suppressor that inhibits the growth of breast, ovary, and lung cancer cells. It is reported that GPC3 can form a complex with insulin-like growth factor 2 (IGF2), and might thereby modulate IGF2 action. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC3 and recombinant rat IGF2. Briefly, GPC3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IGF2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse GPC3 and recombinant rat IGF2 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.)
Glypican 3 (GPC3), Active Protein (Cat# AAA161815)
Full Name
Active Glypican 3 (GPC3)
Gene Names
Gpc3; OCI-5
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Cathepsin D is a lysosomal aspartic protease of the pepsin family. Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 118), a propeptide (residues 1964), and a mature chain (residues 65412). The mature chain can be processed further to the light (residues 65161) and heavy (residues 169412) chains. It is expressed in most cells and overexpressed in breast cancer cells. It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis. The activity of recombinant human CTSD is measured by its ability to cleave a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 0.1 M NaOAc, 0.2 M NaCl, pH 3.5. The rhCTSD is diluted to 20 ug/ml in assay buffer, then incubated at 37 degree C for 30min. The incubated rhCTSD is diluted to 5 ug/mL in assay buffer. Loading into a black well plate 50 uL of 5 ug/mL rhCTSD and start the reaction by adding 50 uL of 60 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhCTSD. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human CTSD is > 675 pmol/min/ug.)
Cathepsin D (CTSD), Active Protein (Cat# AAA161838)
Full Name
Active Cathepsin D (CTSD)
Gene Names
CTSD; CPSD; CLN10
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
B-Cell Receptor CD22 (CD22), Active Protein (Cat# AAA243725)
Full Name
Recombinant Human B-Cell Receptor CD22 (CD22), Partial (Active)
Gene Names
CD22; SIGLEC2; SIGLEC-2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
VEGF120, Active Protein (Cat# AAA79261)
Full Name
Mouse VEGF120
Gene Names
Vegfa; Vpf; Vegf
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
(VEGF-B167 BioLISA using recombinant human soluble Flt-1 for capturing and recombinant human VEGF-B167 as standard. A mouse anti-human VEGF-B antibody in combination with a goat anti-mouse Biotin-conjugated antibody was used for detection.)
VEGF-B167, Active Protein (Cat# AAA79319)
Full Name
Human VEGF-B167
Gene Names
VEGFB; VRF; VEGFL
Reactivity
Human
Purity
> 98% by SDS-PAGE & Coomassie stain
Pricing
SDF-1 alpha (CXCL12), Active Protein (Cat# AAA79145)
Full Name
Human SDF-1 alpha (CXCL12)
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Purity
> 98% by SDS-PAGE & HPLC analyses
Pricing
VEGF120, Active Protein (Cat# AAA79185)
Full Name
Mouse VEGF120
Gene Names
Vegfa; Vpf; Vegf
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
HYALURONIDASE, Active Protein (Cat# AAA50501)
Full Name
NATIVE BOVINE HYALURONIDASE
Applications
ELISA
Pricing
Interleukin-3, Active Protein (Cat# AAA38970)
Full Name
Recombinant Rhesus Macaque Interleukin-3
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Purity
Greater than 97.0% as determined by SDS-PAGE.
Pricing
Granzyme-B, Active Protein (Cat# AAA38045)
Full Name
Recombinant Human Granzyme-B, Sf9
Gene Names
GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Interleukin-8, Active Protein (Cat# AAA38079)
Full Name
Recombinant Human Interleukin-8 (1-72) (CXCL8)
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Monocyte Chemotactic Protein-1, Active Protein (Cat# AAA38086)
Full Name
Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
Gene Names
Ccl2; MCP-1; Scya2; Sigje
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing
Granulocyte-Colony Stimulating Factor, Active Protein (Cat# AAA38114)
Full Name
Recombinant Human Granulocyte-Colony Stimulating Factor
Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
c-AMP dependant Protein Kinase A catalytic subunit alpha, Active Protein (Cat# AAA38382)
Full Name
Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha
Gene Names
PRKACA; PKACA; PPNAD4
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
Follistatin, Active Protein (Cat# AAA76116)
Full Name
Human Follistatin Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Elastase (ELA-2), Active Protein (Cat# AAA44742)
Full Name
Elastase (ELA-2), Human Neutrophils
Purity
> 96% (SDS-PAGE. Multiple bands observed)
Pricing
Bioactivity
(Troponin T Type 2, Cardiac (TNNT2) is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury. Besides, Troponin I Type 3, Cardiac (TNNI3) has been identified as an interactor of TNNT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNT2 and recombinant human TNNTI3. Briefly, TNNT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to TNNI3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNNT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450/630nm immediately. The binding activity of TNNT2 and TNNI3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Troponin T Type 2, Cardiac (TNNT2), Active Protein (Cat# AAA152235)
Full Name
Active Troponin T Type 2, Cardiac (TNNT2)
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
Interleukin 10 (IL10), Active Protein (Cat# AAA146605)
Full Name
Active Interleukin 10 (IL10)
Gene Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
SDS-PAGE
IL22BP / IL22RA2, Active Protein (Cat# AAA173554)
Full Name
Recombinant Human IL22BP / IL22RA2 Protein (His tag)
Gene Names
IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; ZCYTOR16; IL-22R-alpha-2
Purity
> 95 % as determined by reducing SDS-PAGE
Pricing
Application Data
IL13, Active Protein (Cat# AAA214322)
Full Name
Recombinant Mouse IL13
Gene Names
Il13; Il-13
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 ug/ml.)
PCSK9, Active Protein (Cat# AAA258147)
Full Name
PCSK9 Protein, Mouse, Recombinant (His Tag)
Gene Names
Pcsk9; FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized PDGFRbeta-His at 10 ug/ml (100 ul/well) can bind Cynomolgus PDGFB, The EC50 of Cynomolgus PDGFB is 3-8 ng/ml.)
PDGFRB, Active Protein (Cat# AAA257890)
Full Name
PDGFRB Protein, Human, Recombinant (His Tag)
Gene Names
PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
Purity
>90% as determined by SDS-PAGE
Pricing
Dihydrofolate Reductase, Active Protein (Cat# AAA38726)
Full Name
Recombinant Human Dihydrofolate Reductase
Gene Names
DHFR; DYR; DHFRP1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Insulin Like Growth Factor Binding Protein-1, Active Protein (Cat# AAA38149)
Full Name
Human Insulin Like Growth Factor Binding Protein-1
Gene Names
IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Creatine Kinase MB Isoenzyme Type-2, Active Protein (Cat# AAA38100)
Full Name
Recombinant Human Creatine Kinase MB Isoenzyme Type-2
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
EPO, Active Protein (Cat# AAA75440)
Full Name
EPO protein
Gene Names
EPO; EP; MVCD2
Reactivity
Human
Purity
> 98% pure by SDS-PAGE analysis
Pricing
Lactate Dehydrogenase 4, Active Protein (Cat# AAA44812)
Full Name
Lactate Dehydrogenase 4 (LDH-4), Human Liver
Pricing
Granulocyte Macrophage Colony Stimulating Factor (rHuGM-CSF), Active Protein (Cat# AAA47951)
Full Name
Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rHuGM-CSF)
Gene Names
CSF2; GMCSF; MGC131935; MGC138897
Purity
>98% as determined by RPHPLC and SDS-PAGE.
Pricing
CD4, Active Protein (Cat# AAA48979)
Full Name
Recombinant human CD4 protein
Gene Names
CD4; CD4mut
Applications
Bioactivity, SDS-PAGE
Purity
90% by SDS-PAGE
Pricing
CALMODULIN, Active Protein (Cat# AAA50491)
Full Name
NATIVE BOVINE CALMODULIN
Applications
ELISA
Purity
> 95%. Determined by SDS-PAGE.
Pricing
Bioactivity
(Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF is a monomeric glycoprotein that functions as a cytokine - it is a white blood cell growth factor. It also has some effects on mature cells of the immune system, such as inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GM-CSF and recombinant human CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human GM-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.04 ug/mL.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein (Cat# AAA161668)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.)
Nerve Growth Factor (NGF), Active Protein (Cat# AAA161688)
Full Name
Active Nerve Growth Factor (NGF)
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant bovine TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rbTIMP2 (MW: 51.75 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rhTIMP2 curve dilutions, 12.8 ul of activated rbMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant bovine TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161695)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 degree C for 30 minutes. Loading 50 uL 20 uM substrate to start the reaction including a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was )
Serpin A10 (SERPINA10), Active Protein (Cat# AAA161873)
Full Name
Active Serpin A10 (SERPINA10)
Gene Names
SERPINA10; PZI; ZPI
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant dog TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and TIMP1 (MW: 20.86 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of TIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant dog TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161742)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
TIMP1; TIMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 i)
Toll Like Receptor 4 (TLR4), Active Protein (Cat# AAA153056)
Full Name
Active Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not know)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153076)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Gene Names
Vgf; Gm1052
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Application Data
Indoleamine-2,3-Dioxygenase (IDO), Active Protein (Cat# AAA148135)
Full Name
Active Indoleamine-2,3-Dioxygenase (IDO)
Gene Names
IDO1; IDO; INDO; IDO-1
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.)
BID, Active Protein (Cat# AAA257888)
Full Name
BID Protein, Human, Recombinant
Gene Names
BID; FP497
Purity
>90% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.