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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 550-600 of 2875 product results
Transforming Growth Factor-Beta 3, Active Protein (Cat# AAA76535)
Full Name
Transforming Growth Factor-Beta 3, Recombinant Protein
Gene Names
TGFB3; ARVD; LDS5; RNHF; ARVD1; TGF-beta3
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing
CD23/FCER2, Active Protein (Cat# AAA49009)
Full Name
Recombinant human CD23/FCER2 protein
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(IL1alpha(Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1alpha results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1alpha. MCF-7 cells were seeded overnight at a density of 4,000 cells/well, and then treated with or without various concentrations of IL1alpha for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours in at 37 degree C. Inhibition of MCF-7 cell proliferation after incubation with IL1alpha for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1alpha for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1alpha significantly decreased cell viability of MCF-7 cells and the EC50 was 4.69 ug/ml.)
Interleukin 1 Alpha (IL1a), Active Protein (Cat# AAA161677)
Full Name
Active Interleukin 1 Alpha (IL1a)
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Matrix Metalloproteinase 2 (MMP2), Active Protein (Cat# AAA146612)
Full Name
Active Matrix Metalloproteinase 2 (MMP2)
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Pricing
Application Data
FGFR2, Active Protein (Cat# AAA258054)
Full Name
FGFR2 Protein, Human, Recombinant (alpha IIIb, hFc Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to bind human EGF in a functional ELISA.)
EGFR, Active Protein (Cat# AAA258225)
Full Name
EGFR Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
Purity
>90% as determined by SDS-PAGE
Pricing
Complement C5b, 6 complex ag, Active Protein (Cat# AAA75367)
Full Name
Complement C5b, 6 complex protein
Reactivity
Human
Purity
>=98% by SDS-PAGE
Pricing
Application Data
Fibronectin (FN), Active Protein (Cat# AAA148157)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Galectin-6(GAL6) is a protein that in humans is encoded by the LGALS6 gene. The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which)
Galectin 6 (GAL6), Active Protein (Cat# AAA153030)
Full Name
Active Galectin 6 (GAL6)
Gene Names
Lgals6; galectin-6
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Application Data
Interleukin 6 (IL6), Active Protein (Cat# AAA146610)
Full Name
Active Interleukin 6 (IL6)
Gene Names
Il6; Il-6
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Lactate Dehydrogenase, Active Protein (Cat# AAA44807)
Full Name
Lactate Dehydrogenase (LDH), Bovine Heart
Purity
Purified
Pricing
Growth Hormone, Active Protein (Cat# AAA38592)
Full Name
Recombinant Rabbit Growth Hormone Binding Protein
Purity
Greater than 98.0% as determined bySDS-PAGE.
Pricing
Deoxyribonuclease I, Active Protein (Cat# AAA38277)
Full Name
Recombinant Human Deoxyribonuclease I
Gene Names
DNASE1; DNL1; DRNI
Pricing
ICAM-1/CD54, Active Protein (Cat# AAA48983)
Full Name
Recombinant human ICAM-1/CD54 protein
Gene Names
ICAM1; BB2; CD54; P3.58
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
FGF-23, Active Protein (Cat# AAA79235)
Full Name
Recombinant Mouse FGF-23
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & HPLC analyses
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant rat RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rrRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rrRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >22 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161808)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Gene Names
Rbp4; RBPA
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
MIF, Active Protein (Cat# AAA214327)
Full Name
Recombinant Mouse MIF
Gene Names
Mif; GIF; DER6; Glif
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to bind recombinant human S100A9 in a functional ELISA.)
S100A8, Active Protein (Cat# AAA257965)
Full Name
S100A8 Protein, Human, Recombinant (His Tag)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized FGF2 Protein, Human at 2 ug/mL (100 uL/well) can bind Glypican 3 Protein, Human, Recombinant (495S/A, hFc Tag), the EC50 of Glypican 3 is 0.75-1.1 ug/mL.)
Glypican 3, Active Protein (Cat# AAA257828)
Full Name
Glypican 3 Protein, Human, Recombinant (495S/A, hFc Tag)
Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Purity
>95% as determined by SDS-PAGE
Pricing
MMP-1, Active Protein (Cat# AAA79296)
Full Name
Human MMP-1
Gene Names
MMP1; CLG; CLGN
Purity
> 98% by SDS-PAGE & HPLC analysis
Pricing
TRAIL/Apo2L, soluble, Active Protein (Cat# AAA79152)
Full Name
Human TRAIL/Apo2L, soluble
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Reactivity
Human
Purity
> 98% by SDS-PAGE ans HPLC analysis
Pricing
Application Data
VEGF165, Active Protein (Cat# AAA79212)
Full Name
Human VEGF165
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
BD1, Active Protein (Cat# AAA75578)
Full Name
BD1 protein
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Purity
>98% by SDS-PAGE gel and HPLC analysis
Pricing
GALECTIN-1, Active Protein (Cat# AAA50540)
Full Name
RECOMBINANT HUMAN GALECTIN-1
Gene Names
LGALS1; GBP; GAL1
Applications
Western Blot, Functional Assay
Purity
>98% by SDS PAGE/HPLC analysis
Pricing
Interleukin-3, Active Protein (Cat# AAA38782)
Full Name
Recombinant Canine Interleukin-3
Gene Names
IL3; IL-3; MCGF
Purity
Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Pricing
Tyrosine Phosphatase Non Receptor Type-1, Active Protein (Cat# AAA38391)
Full Name
Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-1
Gene Names
PTPN1; PTP1B
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Urokinase, Active Protein (Cat# AAA38242)
Full Name
Human Urokinase
Purity
Greater than 90.0%.
Pricing
Coagulation Factor IX, Active Protein (Cat# AAA38450)
Full Name
Human Coagulation Factor IX
Gene Names
F9; FIX; P19; PTC; HEMB; THPH8
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
CD80 (B7-1), Active Protein (Cat# AAA76080)
Full Name
Human CD80 Recombinant (B7-1)
Purity
>90%, as determined by SDS-PAGE and HPLC
Pricing
beta-Human Chorionic Gonadotrophin, Active Protein (Cat# AAA44799)
Full Name
beta-Human Chorionic Gonadotropin (b-hCG)
Purity
>98% (SDS-PAGE)
Pricing
Bioactivity
(Figure 2. Inhibition of Hepa1-6 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152988)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
Tnf; TNFA; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Glial Cell Line Derived Neurotrophic Factor (GDNF), Active Protein (Cat# AAA149225)
Full Name
Active Glial Cell Line Derived Neurotrophic Factor (GDNF)
Gene Names
Gdnf; ATF; AI385739
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Bioactivity
(Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, Balb/c 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 8, 000 cells/well and allowed to attach overnight, then the medium was replaced with 0.4% FBS standard DMEM prior to the addition of various concentrations of recombinant human EGF. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Balb/c 3T3 cells after incubation with rhEGF for 48h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhEGF was shown in Figure2. It was obvious that rhEGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 4.2 ng/ml.)
Epidermal Growth Factor (EGF), Active Protein (Cat# AAA161748)
Full Name
Active Epidermal Growth Factor (EGF)
Gene Names
EGF; URG; HOMG4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 ng/mL.)
FGF1, Active Protein (Cat# AAA257816)
Full Name
FGF1 Protein, Human, Recombinant
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to bind biotinylated human DMP1 in a functional ELISA.)
Factor H, Active Protein (Cat# AAA257920)
Full Name
Factor H Protein, Human, Recombinant (His Tag)
Gene Names
CFH; FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6. The ED50 for this effect is 50-250 ng/mL.)
CXCL16, Active Protein (Cat# AAA257935)
Full Name
CXCL16 Protein, Human, Recombinant (His Tag)
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Purity
>95% as determined by SDS-PAGE
Pricing
Vascular Endothelial Growth Factor, Active Protein (Cat# AAA38139)
Full Name
Recombinant Rat Vascular Endothelial Growth Factor Related Protein
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF164
Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Neutrophil Activating Protein-2, Active Protein (Cat# AAA38082)
Full Name
Recombinant Human Neutrophil Activating Protein-2 (CXCL7)
Gene Names
PPBP; PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; CTAP-III
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Application Data
VEGF120, Active Protein (Cat# AAA79154)
Full Name
Rat VEGF120
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF164
Reactivity
Rat
Purity
>95%, by SDS-PAGE and silver stain
Pricing
Granzyme B, Active Protein (Cat# AAA79267)
Full Name
Mouse Granzyme B
Gene Names
GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Reactivity
Mouse
Purity
> 97% by SDS-PAGE and HPLC analysis
Pricing
VEGFR-1/Flt-1(D7)-Fc Chimera, soluble, Active Protein (Cat# AAA79121)
Full Name
Human VEGFR-1/Flt-1(D7)-Fc Chimera, soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
PlGF-1/PGF, Active Protein (Cat# AAA48993)
Full Name
Recombinant human PlGF-1/PGF protein
Gene Names
PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
EGF, Active Protein (Cat# AAA49002)
Full Name
Recombinant Animal Free Human EGF protein
Gene Names
EGF; URG; HOMG4
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
VEGFR1/Flt-1, Active Protein (Cat# AAA49007)
Full Name
Recombinant mouse VEGFR1/Flt-1 protein
Gene Names
Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
Applications
Bioactivity, SDS-PAGE
Purity
> 85% by SDS-PAGE
Pricing
Bioactivity
(Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of recombinant human IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.)
Interleukin 5 (IL5), Active Protein (Cat# AAA161678)
Full Name
Active Interleukin 5 (IL5)
Gene Names
IL5; EDF; TRF; IL-5
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant rat TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rrTIMP1 (MW: 22.2 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rrTIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161745)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
Timp1; Timp; TIMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1beta can induced IL-8 production in A549 cells. To test the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with certain concentrations of IL-1beta for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1beta have shown in Figure1.)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA152227)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-3 on TF-1 cells)
Interleukin 3 (IL3), Active Protein (Cat# AAA152968)
Full Name
Active Interleukin 3 (IL3)
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant human MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rhMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 °C for 2 hours. The activated rhMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rhMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human MMP1 is > 140 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA152977)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Gene Names
MMP1; CLG; CLGN
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.