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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 750-800 of 2567 product results
Application Data
D-Lactate dehydrogenase, Active Protein (Cat# AAA78979)
Full Name
D-Lactate dehydrogenase, recombinant from bacteria
Pricing
Bioactivity
(Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
IL-4, Active Protein (Cat# AAA48382)
Full Name
Recombinant human IL-4 protein
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Bioactivity
(Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range )
IL-5, Active Protein (Cat# AAA48401)
Full Name
Recombinant mouse IL-5 Protein
Gene Names
Il5; Il-5
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing conditionandvisualized by coomassie blue stain)
Neprilysin, Active Protein (Cat# AAA48320)
Full Name
Recombinant Neprilysin
Gene Names
MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS - PAGE
Pricing
Bioactivity
(Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is )
IL-10 R alpha, Active Protein (Cat# AAA48321)
Full Name
Recombinant IL-10 R alpha
Gene Names
IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS - PAGE.
Pricing
Bioactivity
(Porcine GM-CSF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ? 40 ng/ml.)
GM-CSF, Active Protein (Cat# AAA48356)
Full Name
Recombinant Porcine GM-CSF protein
Gene Names
CSF2; GM-CSF
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS - PAGE
Pricing
Bioactivity
(Human BMP-2 stimulates alkaline phosphatase in the ATDC5 mouse chondrogenic cells. The ED50 range ? 100 ng/ml.)
BMP-2, Active Protein (Cat# AAA48357)
Full Name
Recombinant human BMP-2 protein
Gene Names
BMP2; BDA2; BMP2A; SSFSC
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)
COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48371)
Full Name
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Bioactivity
(Mouse CD200 is coated at 1 ug/ml (100 ul/well) can bind CD200 R1 (CAT# ATGP3457) in a Functional ELISA assay.)
CD200, Active Protein (Cat# AAA48373)
Full Name
Recombinant mouse CD200 protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Biliverdin reductase A, Active Protein (Cat# AAA48555)
Full Name
Biliverdin reductase A, 3-296aa, Human, E Coli (Bioactivity Validated)
Gene Names
BLVRA; BVR; BLVR; BVRA
Applications
Enzyme Activity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-Page under reducing condition and visualized by coomassie blue stain.)
FKBP3, Active Protein (Cat# AAA48579)
Full Name
FKBP3, 1-224aa, Human, E Coli (Bioactivity Validated)
Gene Names
FKBP3; FKBP-3; FKBP25; PPIase; FKBP-25
Applications
SDS-PAGE, Enzyme Activity
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
PKLR, Active Protein (Cat# AAA48595)
Full Name
PKLR, 47-574aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
PKLR; PK1; PKL; PKR; RPK; PKRL
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
FKBP1a /FKBP12, Active Protein (Cat# AAA48496)
Full Name
FKBP1a /FKBP12, 1-108aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
SDS-PAGE
Fumarase, Active Protein (Cat# AAA48502)
Full Name
Fumarase, 44-510aa, Human, E Coli (Bioactivity Validated)
Gene Names
FH; MCL; FMRD; LRCC; HLRCC; MCUL1
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Peroxiredoxin 2, Active Protein (Cat# AAA48528)
Full Name
Peroxiredoxin 2, 1-198aa, Human, E Coli (Bioactivity Validated)
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a
Applications
Enzyme Activity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
PRDX4, Active Protein (Cat# AAA48624)
Full Name
PRDX4, 38-271aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
SDS-PAGE
CDA, Active Protein (Cat# AAA48626)
Full Name
CDA, 1-146aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
CDA; CDD
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
(Figure 1. Human BCMA, mFc Tag on SDS-PAGE under reducing condition.)
BCMA, Active Protein (Cat# AAA47216)
Full Name
Recombinant human BCMA protein with C-terminal mouse Fc tag
Gene Names
TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
Applications
Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Pricing
SDS-PAGE
Pyrethroid hydrolase Ces2e, Active Protein (Cat# AAA48914)
Full Name
Pyrethroid hydrolase Ces2e, 27-559aa, Mouse, His tag, Baculovirus (Bioactivity Validated)
Gene Names
Ces2e; Ces5; 9030624L02Rik
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
SDS-PAGE
Erythropoietin, Active Protein (Cat# AAA48918)
Full Name
Erythropoietin, 28-193aa, Human, His tag, Baculovirus (Bioactivity Validated)
Gene Names
EPO; EP; MVCD2
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
CD4, Active Protein (Cat# AAA48979)
Full Name
Recombinant human CD4 protein
Gene Names
CD4; CD4mut
Applications
Bioactivity, SDS-PAGE
Purity
90% by SDS-PAGE
Pricing
Fc epsilon RI alpha, Active Protein (Cat# AAA48987)
Full Name
Recombinant Human Fc epsilon RI alpha protein
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
FCRN/FCGRT&B2M, Active Protein (Cat# AAA48991)
Full Name
Recombinant human FCRN/FCGRT&B2M protein
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
EGF, Active Protein (Cat# AAA49002)
Full Name
Recombinant Animal Free Human EGF protein
Gene Names
EGF; URG; HOMG4
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
CD23/FCER2, Active Protein (Cat# AAA49009)
Full Name
Recombinant human CD23/FCER2 protein
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Chorionic Gonadotropin, Active Protein (Cat# AAA59624)
Full Name
Chorionic Gonadotropin, Alpha Subunit, Human
Purity
> or = 95% by SDS-PAGE
Pricing
Luteinizing Hormone, Active Protein (Cat# AAA59626)
Full Name
Luteinizing Hormone, Human
Gene Names
LHB; CGB4; HH23; LSH-B; LSH-beta
Purity
>/= 98% by SDS-PAGE
Pricing
WB (Western Blot)
(Western Blot: NP HIS Tag detected with Covid-19 positive patient serum)
COVID 19 Nucleocapsid (NP) Coronavirus, Active Protein (Cat# AAA58643)
Full Name
Recombinant Human SARS-CoV-2 Nucleoprotein (E. Coli)
Purity
Affinity chromatography. Purity: >95% by SDS PAGE
Pricing
Application Data
VEGF120, Active Protein (Cat# AAA79154)
Full Name
Rat VEGF120
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF164
Reactivity
Rat
Purity
>95%, by SDS-PAGE and silver stain
Pricing
FGFR-2/Fc Chimera, soluble, Active Protein (Cat# AAA79161)
Full Name
Human FGFR-2/Fc Chimera, soluble
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Application Data
(Functional ELISA to test the binding of sTIE-2 to its ligand Ang-2. Ang-2 was coated on the plate and increasing amounts of recombinant human soluble sTIE-2 was added.)
TIE-2, soluble, Active Protein (Cat# AAA79167)
Full Name
Human TIE-2, soluble
Gene Names
TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
VEGFR-1/Flt-1 (D5), soluble, Active Protein (Cat# AAA79171)
Full Name
Human VEGFR-1/Flt-1 (D5), soluble
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human TMED1-His at 10 ug/mL (100 uL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50 ng/mL.)
TMED1, Active Protein (Cat# AAA258020)
Full Name
TMED1 Protein, Human, Recombinant (His Tag)
Gene Names
TMED1; Tp24; Il1rl1l; IL1RL1LG
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2 His at 2 ug/mL (100 uL/well) can bind Human PVRIG mFc, the EC50 of Human PVRIG mFc is 40-260 ng/mL.)
PVRIG, Active Protein (Cat# AAA258061)
Full Name
PVRIG Protein, Human, Recombinant (mFc Tag)
Gene Names
PVRIG; C7orf15
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Interleukin 11 (IL-11) is a multifunctional cytokine. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation IL-11 causes bone-resorption. Besides, IL-11 have been proved can promote migration of A549 cells, 5×104 cells were seeded into 6 well plates. After cell confluent, replace with DMEM without serum overnight. Using a (yellow) pipette tip make a straight scratch, simulating a wound, then washing the wells three times with PBS. Adding 1% serum standard DMEM containing various concentrations of recombinan human IL-11 to each well, incubating the plate for 48 hours at 37, 5% CO2. The results observed by inverted microscope was shown in Figure.(A) A549 cells cultured in DMEM with 100ng/mL IL-11 for 48h; (B) A549 cells cultured in DMEM before addition IL-11.Figure. Wound healing assay of A549 cells after stimulated with IL-11.)
Interleukin 11, Active Protein (Cat# AAA150057)
Full Name
Active Interleukin 11 (IL11)
Gene Names
IL11; AGIF; IL-11
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
WB (Western Blot)
(Sample: Recombinant Fibronectin, Human; Antibody: Rabbit Anti-Human Fibronectin Ab)
Fibronectin (FN), Active Protein (Cat# AAA148156)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing
Application Data
(Interleukin 1 beta (IL-1) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1 can induced IL-8 production in A549 cells. To test the bioactivity of IL-1, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations (0.1ng/mL-1000ng/mL) of IL-1 for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1 have shown in Figure1.Figure. IL-8 levels in the cell supernatant of A549 induced by IL-1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150092)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Sequence
Fibrillin 1, Active Protein (Cat# AAA150097)
Full Name
Active Fibrillin 1 (FBN1)
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing
Bioactivity
(Figure. VEGFR2 inhibit VEGF-dependent proliferation of ECV-304 cells.)
Vascular Endothelial Growth Factor Receptor 2, Active Protein (Cat# AAA150107)
Full Name
Active Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
(Figure. Cell proliferation of Raji cells after stimulated with GP39.Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1) is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 plays a role in cancer cell proliferation, survival, invasiveness and in the regulation of cell-matrix interactions. To test the effect of GP39 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant human GP39. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Proliferation of Raji cells after incubation with GP39 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GP39 for 5 days. The result was shown in Figure 2. It was obvious that GP39 significantly increased cell viability of Raji cells.(A) Raji cells cultured in 1640, stimulated with 10ng/mL GP39 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.)
Chitinase-3-like Protein 1, Active Protein (Cat# AAA150108)
Full Name
Active Chitinase-3-like Protein 1 (CHI3L1)
Gene Names
CHI3L1; GP39; ASRT7; GP-39; YK-40; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
WB (Western Blot)
(Figure. Western Blot)
Transforming Growth Factor Beta 3, Active Protein (Cat# AAA150122)
Full Name
Active Transforming Growth Factor Beta 3 (TGFb3)
Gene Names
TGFB3; ARVD; LDS5; RNHF; ARVD1; TGF-beta3
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure. The binding activity of GaNAB with MYC)
Glucosidase Alpha, Neutral AB, Active Protein (Cat# AAA150131)
Full Name
Active Glucosidase Alpha, Neutral AB (GaNAB)
Gene Names
GANAB; G2AN; GIIA; PKD3; GLUII
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Platelet Factor 4 (PF4), Active Protein (Cat# AAA150559)
Full Name
Active Platelet Factor 4 (PF4)
Gene Names
PF4; PF-4; CXCL4; SCYB4
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
WB (Western Blot)
(WB Image)
Angiotensin I Converting Enzyme 2 (ACE2), Active Protein (Cat# AAA150563)
Full Name
Active Angiotensin I Converting Enzyme 2 (ACE2)
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of woundsIt can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Ra GAL7 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL7 is 25 ug/mL.)
Galectin 7 (GAL7), Active Protein (Cat# AAA152173)
Full Name
Active Galectin 7 (GAL7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TLR2 is a member of TLR family which is type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor that contains)
Toll Like Receptor 2 (TLR2), Active Protein (Cat# AAA153053)
Full Name
Active Toll Like Receptor 2 (TLR2)
Gene Names
TLR2; TIL4; CD282
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Epithelial Neutrophil Activating Peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines. Full-length ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. ENA-78 is expressed by many immune cells, such as macrop)
Epithelial Neutrophil Activating Peptide 78 (ENA78), Active Protein (Cat# AAA153064)
Full Name
Active Epithelial Neutrophil Activating Peptide 78 (ENA78)
Gene Names
Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 (assay buffer), initiated by addition 50 ul of 3 ug/ml ACP1 (diluted by assay buffer) to 50 uL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 ul of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/ug.)
Acid Phosphatase 1 (ACP1), Active Protein (Cat# AAA161821)
Full Name
Active Acid Phosphatase 1 (ACP1)
Gene Names
Acp1; Acp-1; LMW-PTP; AI427468; 4632432E04Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation. It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The activity of recombinant human TFPI was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 20 ul different concentrations of recombinant human TFPI (MW: 61.9 KD) was incubated with 20 ul diluted trypsin at 37 degree C for 15 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 20 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TFPI significantly decreased trypsin activity. The inhibition IC50 was )
Tissue Factor Pathway Inhibitor (TFPI), Active Protein (Cat# AAA161730)
Full Name
Active Tissue Factor Pathway Inhibitor (TFPI)
Gene Names
TFPI; EPI; TFI; LACI; TFPI1
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152987)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
LITAF; TNF-alpha
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.