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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2100-2150 of 2875 product results
Application Data
(CyaA accelerates cell detachment and migration of TLR-activated DCs.Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.)
MIP-3 beta (CCL19), Active Protein (Cat# AAA76385)
Full Name
Mouse MIP-3 beta (CCL19) Recombinant
Gene Names
Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
Purity
>98%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B)
B-Lymphocyte Activation Antigen B7-1 (LAB7-1), Active Protein (Cat# AAA153084)
Full Name
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(PECAM-1 (platelet-endothelial cell adhesion molecule-1; also known as CD31) is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily. Expression is restricted to cells involved in circulation, especially endotheli)
Platelet/Endothelial Cell Adhesion Molecule (PECAM1), Active Protein (Cat# AAA153034)
Full Name
Active Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5)
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA153069)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure. The binding activity of FGF2 with CASP1.)
Fibroblast Growth Factor 2, Basic, Active Protein (Cat# AAA150090)
Full Name
Active Fibroblast Growth Factor 2, Basic (FGF2)
Gene Names
Fgf2; Fgfb; bFGF; Fgf-2
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Figure. The binding activity of PTEN with TP53.Phosphatase and tensin homolog (PTEN) is a protein is widely expressed throughout the body. PTEN protein acts as a phosphatase to dephosphorylate phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5)P3 or PIP3). PTEN specifically catalyses the dephosphorylation of the 3’ phosphate of the inositol ring in PIP3, resulting in the biphosphate product PIP2 (PtdIns (4,5) P2). PTEN also has weak protein phosphatase activity, but this activity is also crucial for its role as a tumor suppressor. PTENs protein phosphatase activity may be involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of PTEN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PTEN and recombinant human TP53. Briefly, PTEN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to TP53-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PTEN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PTEN and TP53 was shown in Figure 1, and this effect was in a dose dependent manner.)
Phosphatase And Tensin Homolog, Active Protein (Cat# AAA150140)
Full Name
Active Phosphatase And Tensin Homolog (PTEN)
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Application Data
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA146620)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(GSTa1 (Glutathione S-transferase a1) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant human GSTa1, mix gentliy. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human GSTa1 is >17000 pmol/min/ug.)
Glutathione S Transferase Alpha 1 (GSTa1), Active Protein (Cat# AAA161754)
Full Name
Active Glutathione S Transferase Alpha 1 (GSTa1)
Gene Names
GSTA1; GST2; GTH1; GSTA1-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Band 3 (BND3), also known as Anion Exchanger 1 and SLC4A1, is a 90-100 kDa polytropic membrane protein that is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it mediates the exchange of the cellular HCO3- with CI- in plasma. BND3 is required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Scaffold protein Ankyrin 3, Node Of Ranvier (ANK3) can Interacts with BND3, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat BND3 and recombinant human ANK3. Briefly, biotin-linked BND3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ANK3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat BND3 and recombinant human ANK3 was shown in Figure 1, the EC50 for this effect is 0.35 ug/mL.)
Band 3 (BND3), Active Protein (Cat# AAA161856)
Full Name
Active Band 3 (BND3)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
SARS-CoV S1 Protein, Active Protein (Cat# AAA177983)
Full Name
Recombinant SARS-CoV S1 Protein (mFc Tag)(Active)
Purity
>90% as determined by SDS-PAGE
Pricing
Growth Hormone, Active Protein (Cat# AAA38548)
Full Name
Recombinant Porcine Growth Hormone
Gene Names
GH1; GH
Purity
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Bioactivity
(Human IL-9 stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )
IL-9, Active Protein (Cat# AAA48322)
Full Name
Recombinant IL-9
Gene Names
IL9; P40; HP40; IL-9
Applications
Bioactivity, SDS-PAGE
Purity
> 85% by SDS - PAGE
Pricing
SDS-PAGE
SHP2 / PTPN11, Active Protein (Cat# AAA173592)
Full Name
Recombinant Mouse SHP2 / PTPN11 Protein (His tag)
Gene Names
Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
Purity
> 90 % as determined by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)
Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161666)
Full Name
Active Colony Stimulating Factor 3, Granulocyte (GCSF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 5 (C5), which forms part of the later stages of the complement pathway, is cleaved into C5a and C5b. C5a is a chemotactic agent for neutrophils, while C5b forms part of the complement membrane attack complex. Coagulation Factor II (F2) has been identified as an interactor of C5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C5 and recombinant mouse F2. Briefly, C5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C5 and recombinant mouse F2 was shown in Figure 1, the EC50 for this effect is 0.22 ug/mL.)
Complement Component 5 (C5), Active Protein (Cat# AAA161871)
Full Name
Active Complement Component 5 (C5)
Gene Names
C5; C5a; C5b; CPAMD4
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Apolipoprotein A1 (APOA1) is the major protein component of HDL particles in plasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. Besides, Plasminogen Activator, Urokinase (uPA) has been identified as an interactor of APOA1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOA1 and recombinant human uPA. Briefly, biotin-linked APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to uPA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human uPA and recombinant human APOA1 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.)
Apolipoprotein A1 (APOA1), Active Protein (Cat# AAA161738)
Full Name
Active Apolipoprotein A1 (APOA1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Interleukin 29 (IL29), Active Protein (Cat# AAA149255)
Full Name
Active Interleukin 29 (IL29)
Gene Names
IFNL1; IL29; IL-29
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(The interleukin-1 receptor antagonist (IL-1RA) is a member of the interleukin 1 cytokine family. IL1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effec)
Interleukin 1 Receptor Antagonist (IL1RA), Active Protein (Cat# AAA153008)
Full Name
Active Interleukin 1 Receptor Antagonist (IL1RA)
Gene Names
IL1RN; IRAP; IL-1RN; IL-1ra
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Programmed Cell Death 1 Ligand 2 (also known as PD-L2, B7-DC) is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance. Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) wit)
Programmed Cell Death Protein 1 Ligand 2 (PDL2), Active Protein (Cat# AAA153059)
Full Name
Active Programmed Cell Death Protein 1 Ligand 2 (PDL2)
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
Interleukin 4 (IL4), Active Protein (Cat# AAA146607)
Full Name
Active Interleukin 4 (IL4)
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human TMED1-His at 10 ug/mL (100 uL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50 ng/mL.)
TMED1, Active Protein (Cat# AAA258020)
Full Name
TMED1 Protein, Human, Recombinant (His Tag)
Gene Names
TMED1; Tp24; Il1rl1l; IL1RL1LG
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human VEGF121 at 10 ug/mL (100 uL/well) can bind human VEGFR2-Fc, the EC50 of human VEGFR2-Fc is 5-50 ng/mL.)
VEGF121, Active Protein (Cat# AAA257813)
Full Name
VEGF121 Protein, Human, Recombinant
Gene Names
VEGFA; VPF; VEGF; MVCD1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 (BA.2.75.2) Spike RBD Protein (His Tag)(Cat: AAA258583), the EC50 is 20-60 ng/mL.)
COVID 19 BA.2.75.2 (Omicron) Spike RBD Coronavirus, Active Protein (Cat# AAA258583)
Full Name
SARS-CoV-2 BA.2.75.2 (Omicron) Spike RBD Protein (His Tag)
Purity
>95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
Pricing
Bioactivity
(Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MIg-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SPC with MIg.)
Surfactant Protein C, Active Protein (Cat# AAA150111)
Full Name
Active Surfactant Protein C (SP-C)
Gene Names
Sftpc; SP-C
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5)
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA153070)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Fibroblast Growth Factor 2 (FGF2), Active Protein (Cat# AAA149236)
Full Name
Active Fibroblast Growth Factor 2, Basic (FGF2)
Gene Names
FGF2; BFGF; FGF-2; HBGF-2
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Bioactivity
TSLP, Active Protein (Cat# AAA177943)
Full Name
Recombinant Human TSLP (C-10His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
(NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), also known as DT-diaphorase, is a widely-distributed FAD-dependent flavoprotein that promotes 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes. As a result it prevents the one electron reduction of quinones that results in the production of radical species. NQO1 is a highly-inducible enzyme that is regulated by the Keap1/Nrf2/ARE pathway. The increase and decrease of NQO1 levels are associated with decreased and increased susceptibilities to oxidative stress, respectively. Thus, NQO1 is a marker cytoprotective enzyme in oxidative stress. Independently of its catalytic function, NQO1 plays a role in regulating the proteosomal degradation of p53, p73a, and p33. NQO1 physically interacts with p53 and p73 in an NADH-dependent manner and protects them from 20S proteasomal degradation in a ubiquitin independent pathway. The activity assay of recombinant human NQO1 was measured by its ability to oxidize the substrate resazurin to resorufin. The rhNQO1 was diluted to 100 ug/ml in the assay buffer 50 mM HEPES, 0.2 M NaCl, 5 uM FAD, 0.05% Tween 20, pH 7.5. 50 ul 100 ug/ml rhNQO1 was added into the microplate and start the reaction by adding 50 ul substrate mixture of 400 uM beta-NADH and 20 uM resazurin which was diluted in assay buffer. Read at excitation and emission wavelengths of 540 nm and 585 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human NQO1 is >18 pmol/min/ug.)
NADH Dehydrogenase, Quinone 1 (NQO1), Active Protein (Cat# AAA161938)
Full Name
Active NADH Dehydrogenase, Quinone 1 (NQO1)
Gene Names
NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Coagulation Factor IX, also known as Christmas Factor, is a secreted by the liver and plays a key role in the activation of the intrinsic clotting cascade. Factor IX consists of a Gla domain, two tandem EGF-like domains, an activation peptide, and an S1 serine protease domain. Mature human Factor IX shares approximately 81% amino acid sequence identity with mouse and rat Factor IX. Alternative splicing generates an additional isoform that lacks the first EGF-like domain. The activity of recombinant rabbit Coagulation Factor IX is measured by its ability to cleave a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 8.0. The Coagulation Factor IX is diluted to 100 ug/ml in assay buffer, then activated with a final concentration of 10 ug/ml Thermolysin at 37 degree C for 90min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated Coagulation Factor IX is diluted to 6 ug/mL in assay buffer. Loading into a black well plate 50 uL of 6 ug/mL Coagulation Factor IX and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no Coagulation Factor IX. Then read at 320/405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rabbit Coagulation Factor IX is > 20 pmol/min/ug.)
Coagulation Factor IX (F9), Active Protein (Cat# AAA161786)
Full Name
Active Coagulation Factor IX (F9)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Application Data
TFF2, Active Protein (Cat# AAA214282)
Full Name
Recombinant Human TFF2
Gene Names
TFF2; SP; SML1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 7-30 ng/mL.)
Flt3 Ligand, Active Protein (Cat# AAA258233)
Full Name
Flt3 Ligand Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Flt3l; Ly72L; Flt3lg
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(PLA2G-VII which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s,)
Phospholipase A2 Group VII (LpPLA2), Active Protein (Cat# AAA153065)
Full Name
Active Phospholipase A2 Group VII (LpPLA2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure. The binding activity of TAT with HSPA8)
Tyrosine Aminotransferase, Active Protein (Cat# AAA150132)
Full Name
Active Tyrosine Aminotransferase (TAT)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS-PAGE
Plasminogen Activator, Tissue (tPA), Active Protein (Cat# AAA149233)
Full Name
Active Plasminogen Activator, Tissue (tPA)
Gene Names
Plat; tPA; AU020998; AW212668; D8Ertd2e
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rabit CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rabbit CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161785)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Carcinoembryonic antigen (CEA), as one of the common tumor markers, is a human glycoprotein involved in cell adhesion and is expressed during human fetal development. Since the birth of human, CEA expression is largely inhibited, with only low levels in the plasma of healthy adults. Generally, CEA will overexpressed in many cancers, including gastric, breast, ovarian, lung, and pancreatic cancers, especially colorectal cancer. The lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is also a secreted, which was first identified as cancer and metastasis associated protein. LGALS3BP has been identified as an interactor of CEA, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CEA and recombinant human LGALS3BP. Briefly, biotin-linked CEA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LGALS3BP-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of CEA and LGALS3BP was shown in Figure 1, and this effect was in a dose dependent manner.)
Carcinoembryonic Antigen (CEA), Active Protein (Cat# AAA161710)
Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
HER3 / ErbB3, Active Protein (Cat# AAA173572)
Full Name
Recombinant Human HER3 / ErbB3 Protein (His tag)
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Purity
> 95 % as determined by SDS-PAGE
Pricing
Bioactivity
Signal Transducer CD24/CD24 Biotinylated, Active Protein (Cat# AAA177962)
Full Name
Recombinant Human Signal Transducer CD24/CD24 (C-Fc-Avi) Biotinylated
Gene Names
CD24; CD24A
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Application Data
CXCL1, Active Protein (Cat# AAA214328)
Full Name
Recombinant Mouse CXCL1
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.)
FGF2, Active Protein (Cat# AAA258121)
Full Name
FGF2 Protein, Mouse, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Activity
(Phospholipase A2 Group VII (PLA2G7) which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets and catalyzes the degradation of platelet-activating factor to biologically inactive products. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PLA2G7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PLA2G7 and recombinant rat PON1. Briefly, PLA2G7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PON1-coated microtiter wells and incubated for 1h at 37?. Wells were washed with PBST and incubated for 1h with anti-PLA2G7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37?. Finally, add 50uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PLA2G7 and recombinant rat PON1 was shown in Figure 1, the EC50 for this effect is 0.7 ug/mL)
Phospholipase A2, Lipoprotein Associated (LpPLA2), Active Protein (Cat# AAA21141)
Full Name
Active Phospholipase A2, Lipoprotein Associated (LpPLA2)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Applications
Cell Culture, Activity Assay
Purity
>95%
Pricing
WB (Western Blot)
(Western Blot: NP HIS Tag detected with Covid-19 positive patient serum)
COVID 19 Nucleocapsid (NP) Coronavirus, Active Protein (Cat# AAA58643)
Full Name
Recombinant Human SARS-CoV-2 Nucleoprotein (E. Coli)
Purity
Affinity chromatography. Purity: >95% by SDS PAGE
Pricing
Alpha-Amylase, Active Protein (Cat# AAA44779)
Full Name
a-Amylase, Human Saliva
Gene Names
AMY1A; AMY1
Purity
>= 90% (SDS-PAGE)
Pricing
SDS-PAGE
Carbonic Anhydrase IX / CA9, Active Protein (Cat# AAA173576)
Full Name
Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His tag)
Gene Names
CA9; MN; CAIX
Purity
> 95 % as determined by SDS-PAGE
Pricing
Bioactivity
(Ephrin-A4, also known as EFNA4 and EFL-4, is a member of the ligand of the EPH family. It is mainly expressed in the spleen, lymph nodes, ovary, small intestine and colon of adults, as well as in the heart, lungs, liver, and kidneys of the fetus. It is involved in the development of neurons, blood vessels, and epithelium by regulating cell migration, rejection, and adhesion. Ephrin-A4 has been shown to bind FYN,EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat EFNA4 and recombinant human FYN. Briefly, EFNA4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FYN-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat EFNA4 and recombinant human FYN was shown in Figure 1, the EC50 for this effect is 0.37 ug/mL.)
Ephrin A4 (EFNA4), Active Protein (Cat# AAA161918)
Full Name
Active Ephrin A4 (EFNA4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor. It is primarily secreted by macrophages and monocytes and can form disulfide-linked multimers. Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP. It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides. It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance. A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion. It also inhibits proteasome activity in activated endothelial cells. The activity of recombinant mouse PAI-2 was measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) in the assay buffer 50 mM Tris, 0.01% Tween 20, pH 8.5. The 50 ul different concentrations of rmPAI-2 (MW: 47.9 KD) was incubated with 50ul 2ug/ml rhuPA (EPA140Mu61) at room temperature for 15 minutes. Loading 50 uL of the incubated mixtures into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate (Z-GGR-AMC). Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that PAI2 significantly decreased uPA activity. The inhibition IC50 was )
Plasminogen Activator Inhibitor 2 (PAI2), Active Protein (Cat# AAA161740)
Full Name
Active Plasminogen Activator Inhibitor 2 (PAI2)
Gene Names
Serpinb2; PAI-2; Planh2; ovalbumin
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 4b (C4b) is a component of the complement system, which is activated by the recognition of foreign pathogens, such as bacteria and viruses, or altered self-cells. In the classical activation pathway of complement, C4b is produced by the proteolytic cleavage of the precursor protein C4 by the activated enzyme C1s, and it can bind to C2a to form C3 invertase (C4b2a) which is responsible for the cleavage of C3. Besides, the binding of MASP2 to C4b is an important step in the lectin pathway of the complement system, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C4b and recombinant human MASP2. Briefly, C4b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MASP2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C4b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C4b and recombinant human MASP2 was shown in Figure 1, the EC50 for this effect is 1.39 ug/mL.)
Complement C4-B (C4B), Active Protein (Cat# AAA161839)
Full Name
Active Complement C4-B (C4B)
Gene Names
C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Sample: Recombinant S100A8, Human;Antibody: Rabbit Anti-Human S100A8 Ab)
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA149250)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Interleukin-29 (IL-29) is a member of the helical cytokine family and is a type III interferon. It is also known as IFNlambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host)
Interleukin 29 (IL29), Active Protein (Cat# AAA153110)
Full Name
Active Interleukin 29 (IL29)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Ciliary Neurotrophic Factor (CNTF) is a common extracellular polypeptide hormone who has neuroprotective effects on a variety of central and also peripheral nervous system neurons. It promotes differentiation and maturation of oligodendrocyte precursor ce)
Ciliary Neurotrophic Factor (CNTF), Active Protein (Cat# AAA152949)
Full Name
Active Ciliary Neurotrophic Factor (CNTF)
Gene Names
CNTF; HCNTF
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.