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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2250-2300 of 2583 product results
Application Data
TNFSF15, Active Protein (Cat# AAA214297)
Full Name
Recombinant Human TNFSF15
Gene Names
TNFSF15; TL1; TL1A; VEGI; TNLG1B; VEGI192A
Pricing
Application Data
IL11, Active Protein (Cat# AAA214315)
Full Name
Recombinant Mouse IL11
Gene Names
Il11; IL-11
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
MIF, Active Protein (Cat# AAA214327)
Full Name
Recombinant Mouse MIF
Gene Names
Mif; GIF; DER6; Glif
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CXCL2, Active Protein (Cat# AAA214329)
Full Name
Recombinant Mouse CXCL2
Gene Names
Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL17, Active Protein (Cat# AAA214331)
Full Name
Recombinant Mouse IL17
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Beta-Defensin 1 (DEFB1), Active Protein (Cat# AAA214212)
Full Name
Recombinant Human Beta-Defensin 1 (DEFB1)
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Beta-Defensin 2 (DEFB4A), Active Protein (Cat# AAA214213)
Full Name
Recombinant Human Beta-Defensin 2 (DEFB4A)
Gene Names
DEFB4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CD40 Ligand (CD40LG), Active Protein (Cat# AAA214215)
Full Name
Recombinant Human CD40 Ligand (CD40LG)
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
HCC-1 (CCL14), Active Protein (Cat# AAA214225)
Full Name
Recombinant Human HCC-1 (CCL14)
Gene Names
CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
PDGFB, Active Protein (Cat# AAA214227)
Full Name
Recombinant Human PDGFB
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL18, Active Protein (Cat# AAA214234)
Full Name
Recombinant Human IL18
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL2, Active Protein (Cat# AAA214235)
Full Name
Recombinant Human IL2
Gene Names
IL2; IL-2; TCGF; lymphokine
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL33, Active Protein (Cat# AAA214237)
Full Name
Recombinant Human IL33
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
LIF, Active Protein (Cat# AAA214240)
Full Name
Recombinant Human LIF
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
MCP-2 (CCL8), Active Protein (Cat# AAA214243)
Full Name
Recombinant Human MCP-2 (CCL8)
Gene Names
CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CCL3, Active Protein (Cat# AAA214246)
Full Name
Recombinant Human CCL3
Gene Names
CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CCL4, Active Protein (Cat# AAA214247)
Full Name
Recombinant Human CCL4
Gene Names
CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
MIP-3 (CCL23), Active Protein (Cat# AAA214248)
Full Name
Recombinant Human MIP-3 (CCL23)
Gene Names
CCL23; CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Neutrophil Activating Protein 3 (NAP3),also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, arteriogenesis, inflammation, wound healing, and tumorigenesis. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 5×105 cells/ml in RPMI 1640 with FBS free) and recombinant human NAP3 (0.01ng/mL, 0.1ng/mL, 1ng/mL, 10ng/mL, 100ng/mL, 1000ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×200) randomly (five fields for each filter). Result shows recombinant human NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification(×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification(×200). Statistical results were shown in Figure 2. The optimum chemotaxis of NAP3 occurs at 0.1~1ng/mL.)
Chemokine (C-X-C Motif) Ligand 1 (CXCL1), Active Protein (Cat# AAA152237)
Full Name
Active Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and mo)
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Active Protein (Cat# AAA152958)
Full Name
Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Gene Names
CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152990)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFa
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell apoptosis of A549 cells after stimulated with recombinant dog IFNb.)
Interferon Beta (IFNb), Active Protein (Cat# AAA153001)
Full Name
Active Interferon Beta (IFNb)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell apoptosis of A549 cells after stimulated with recombinant rat IFNb.)
Interferon Beta (IFNb), Active Protein (Cat# AAA153006)
Full Name
Active Interferon Beta (IFNb)
Gene Names
Ifnb1; Ifnb
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 3 receptor alpha (IL3Ra), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity mouse IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)
Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153092)
Full Name
Active Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
Il3ra; CD123; SUT-1; CDw123
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in l)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153098)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin-6 receptor (IL-6R) is a receptor for IL-6, belonging to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. IL-6)
Interleukin 6 Receptor (IL6R), Active Protein (Cat# AAA153104)
Full Name
Active Interleukin 6 Receptor (IL6R)
Gene Names
IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. H2O2 will have specific absorbance at 240 nm . when wi)
Catalase (CAT), Active Protein (Cat# AAA153116)
Full Name
Active Catalase (CAT)
Gene Names
Cat; Cas1; Cs-1; Cas-1; 2210418N07
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37 degree C. The specific activity of recombinant human CES3 is 700 pmol/min/ug.)
Carboxylesterase 3 (CES3), Active Protein (Cat# AAA153133)
Full Name
Active Carboxylesterase 3 (CES3)
Gene Names
CES3; ES31
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37 degree C. The specific activity of recombinant mouse CES3 is 900 pmol/min/ug.)
Carboxylesterase 3 (CES3), Active Protein (Cat# AAA153134)
Full Name
Active Carboxylesterase 3 (CES3)
Gene Names
Ces1d; TGH; Ces3
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.)
Galectin 2 (GAL2), Active Protein (Cat# AAA153014)
Full Name
Active Galectin 2 (GAL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. It is a heterotrimer that consists of two alpha 1 chains and one alpha 2 chain, although homotrimers consisting of three iden)
Collagen Type I Alpha 1 (COL1a1), Active Protein (Cat# AAA153033)
Full Name
Active Collagen Type I Alpha 1 (COL1a1)
Gene Names
COL1A1; OI4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-beta is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many)
Fibroblast Growth Factor 2, Basic (FGF2), Active Protein (Cat# AAA153039)
Full Name
Active Fibroblast Growth Factor 2, Basic (FGF2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153045)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
IL-1B; IL1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decrea)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA153054)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Gene Names
FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1beta which promote migration and recruitment)
Osteopontin (OPN), Active Protein (Cat# AAA153066)
Full Name
Active Osteopontin (OPN)
Gene Names
Spp1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Motility Related Protein (MRP1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. It can enable ATPase-coupled inorganic anion transmembrane transporter activity. Involved in anion transmembrane transport; renal tubular secretion)
Motility Related Protein (MRP1), Active Protein (Cat# AAA153072)
Full Name
Active Motility Related Protein (MRP1)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant bovine BMP4)
Bone Morphogenetic Protein 4 (BMP4), Active Protein (Cat# AAA161652)
Full Name
Active Bone Morphogenetic Protein 4 (BMP4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant human BMP4)
Bone Morphogenetic Protein 4 (BMP4), Active Protein (Cat# AAA161654)
Full Name
Active Bone Morphogenetic Protein 4 (BMP4)
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant GZMK, Human)
Granzyme K (GZMK), Active Protein (Cat# AAA161446)
Full Name
Active Granzyme K (GZMK)
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
SDS_PAGE
(Sample: Active recombinant CAPN1, Human)
Calpain 1 (CAPN1), Active Protein (Cat# AAA161456)
Full Name
Active Calpain 1 (CAPN1)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS_PAGE
(Sample: Active recombinant GZMK, Mouse)
Granzyme K (GZMK), Active Protein (Cat# AAA161832)
Full Name
Active Granzyme K (GZMK)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
(Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGB. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGB and recombinant mouse FGA. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FGB and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Fibrinogen Beta Chain (FGB), Active Protein (Cat# AAA161833)
Full Name
Active Fibrinogen Beta Chain (FGB)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Elastin (ELN) is an extracellular matrix (ECM) protein responsible for the extensibility and elastic recoil of many vertebrate tissues. ELN is abundant in elastic tissues, and tissues rich in elastin include the aorta and major blood vessels, the lungs, elastic ligaments, tendons and the skin. ELN can bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS), autosomal dominant cutis laxa. Decorin (DCN), an extracellular matrix (ECM) protein, is one of targets of ECM. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse ELN and recombinant human DCN. Briefly, ELN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to DCN-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ELN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse ELN and recombinant human DCN was shown in Figure 1, the EC50 for this effect is 0.068 ug/mL.)
Elastin (ELN), Active Protein (Cat# AAA161843)
Full Name
Active Elastin (ELN)
Gene Names
Eln; AI385707; AI480567; E030024M20Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant human NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant human NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 uL of the different concentrations of Fe(DHBA)3 and 50 uL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant human Lipocalin-2 can bind >8 uM of Fe(DHBA)3.)
Neutrophil gelatinase-associated lipocalin (NGAL), Active Protein (Cat# AAA161845)
Full Name
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) segment and a 41 aa cytoplasmic tail. The ECD contains one C2type and one Vtype Iglike domain. EMMPRIN is expressed in areas of tissue remodeling, including endometrium, placenta, skin, and regions undergoing angiogenesis. It is also expressed on cells with high metabolic activity, such as lymphoblasts, macrophages and particularly tumor cells. A functional ELISA assay was conducted to detect the interaction of recombinant rat EMMPRIN/CD147 and recombinant human SLC3A2. Briefly, CD147 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SLC3A2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD147 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat CD147 and recombinant human SLC3A2 was shown in Figure 1, the EC50 for this effect is 0.82 ug/mL.)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA161852)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin-32 (IL-32) is an interleukin cytokine usually linked to inflammation. The IL-32 transcript is expressed in various human tissues and organs such as the spleen, thymus, leukocyte, lung, small intestine, colon, prostate, heart, placenta, liver, muscle, kidney, pancreas, and brain. Cytokines are critical components of cell signaling pathways that are involved in the regulation of cell growth, metabolism, hormone signaling, immune regulation and a variety of other physiological functions. FGFR1 has been identified as an interactor of IL-32, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL-32 and recombinant human FGFR1. Briefly, IL-32 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL-32 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IL-32 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.15 ug/mL.)
Interleukin 32 (IL32), Active Protein (Cat# AAA161861)
Full Name
Active Interleukin 32 (IL32)
Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(GSTa2 (Glutathione S-transferase a2) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant mouse GSTa2, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant mouse GSTa2 is >50000 pmol/min/ug.)
Glutathione S Transferase Alpha 2 (GSTa2), Active Protein (Cat# AAA161869)
Full Name
Active Glutathione S Transferase Alpha 2 (GSTa2)
Gene Names
Gsta2; Gstc2; Gst2-2; Gstc-2
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Telomerase reverse transcriptase (TERT) is a subunit of the enzyme complex telomerase, which adds nucleotides to the ends of telomeres as they become shortened during cell division .Telomerase complex plays a key role in cancer formation by telomere dependent or independent mechanisms. According to statistics, human telomerase reverse transcriptase (h-TERT) is overexpressed in more than 85% of tumors with diverse histologies, with little expression in normal tissues. Expression of h-TERT correlates with activity of telomerase, which is required for the capacity for limitless replication, a hallmark of cancer. The X-Ray Repair Cross Complementing 6 (XRCC6) is high affinity receptor for TERT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TERT and recombinant rat XRCC6. Briefly, TERT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to XRCC6-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TERT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant TERT and recombinant rat XRCC6 was shown in Figure 1, the EC50 for this effect is 0.009 ug/mL.)
Telomerase Reverse Transcriptase (TERT), Active Protein (Cat# AAA161880)
Full Name
Active Telomerase Reverse Transcriptase (TERT)
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(VCP is an ATPase Associated with diverse cellular Activities (AAA) that has a key role in maintaining cellular homeostasis. VCP is ubiquitously expressed in tissues. At cellular level, VCP functions in many cellular compartments; VCP localizes mainly in the cytoplasm, while a smaller fraction binds to organelles or localizes in the nucleus, where it is implicated in different pathways. VCP assembles and acts as a homo-hexamer, in which each monomer is structured in an N-terminal domain that interacts with adaptors and co-factors; in two ATPase domains, D1 and D2, that by hydrolyzing ATP concur, respectively, in the hexamer formation and in supporting VCP activity; and in a C-terminal domain that binds to a small subset of co-factors and adaptors cooperating with D2 activity. The main role of VCP is to recognize and to extract ubiquitinated proteins from membranes, protein complexes, protein aggregates, or chromatin, modulating their ubiquitination or de-ubiquitination, and to enhance their degradation through the UPS or the autophagic pathway. The activity of recombinant human VCP was measured by its ability to hydrolyze the substrate ATP to phosphate which was detected by the malachite green phosphate detection Kit (Beyotime # S0196M). The reaction was performed in the assay buffer 20 mM HEPES, 13 mM NaCl and 1% glycerol, pH 8.5, initiated by addition 27 uL of various concentrations of VCP (diluted by assay buffer) to 3 uL of 100 mM ATP and 100 mM MgCl2 mixture. The final well serves as a negative control with no VCP, replaced with 27 uL assay buffer. Incubated at 37 degree C for 5min, then stop the reaction by quickly freezing samples in the dry ice and ethanol. 4 ul samples was added to 196 ul assay buffer after thawing at room temperature followed by adding 70 ul phosphate detection agent and incubated at room temperature for 30min, then read at a wavelength of 630 nm. The specific activity of recombinant human VCP is >1000 pmol/min/ug.)
Valosin Containing Protein (VCP), Active Protein (Cat# AAA161891)
Full Name
Active Valosin Containing Protein (VCP)
Gene Names
VCP; p97; TERA; ALS14; IBMPFD
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant sheep TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and roTIMP1 (MW: 23.2 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of roTIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant sheep TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161744)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
TIMP1; TIMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.