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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2050-2100 of 2875 product results
Application Data
Fractalkine (CX3CL1), Active Protein (Cat# AAA214279)
Full Name
Recombinant Human Fractalkine (CX3CL1)
Gene Names
CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant rat GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant rat GSTa3 is >37000 pmol/min/ug.)
Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161768)
Full Name
Active Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta1; Yc1; Gsta3; Gsta5; GST AA; GST 2-2; GST Yc1; GST A3-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(C reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant dog CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant dog CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.82 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161778)
Full Name
Active C Reactive Protein (CRP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), Active Protein (Cat# AAA243733)
Full Name
Recombinant Human Tumor-Associated Calcium Signal Transducer 2 (TACSTD2), Partial (Active)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
(Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 ug/mL (100 uL/well) can bind Recombinant Recombinant SARS-CoV-2 (BA.2.3.20) Spike RBD Protein (His Tag)(Cat: AAA258589), the EC50 is 6-18 ng/mL.)
COVID 19 (BA.2.3.20) Spike RBD Coronavirus, Active Protein (Cat# AAA258589)
Full Name
SARS-CoV-2 (BA.2.3.20) Spike RBD Protein (His Tag)
Purity
>95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
Pricing
Application Data
(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 1-6 ug/mL in the presence of 20 ng/mL of Wnt3a.)
SFRP4, Active Protein (Cat# AAA257921)
Full Name
SFRP4 Protein, Human, Recombinant (His Tag)
Gene Names
SFRP4; FRP-4; FRPHE; sFRP-4
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human uPA at 5 ug/ml (100 ul/well) can bind mouse PLAUR with a linear ranger of 1.6-40 ng/ml.)
uPAR/PLAUR, Active Protein (Cat# AAA258134)
Full Name
uPAR/PLAUR Protein, Mouse, Recombinant (His & hFc Tag)
Gene Names
Plaur; Cd87; uPAR; u-PAR
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 ug/mL (100 uL/well) can bind canine EFNB2-Fc3. The EC50 of canine EFNB2-Fc3 is 25.8-60.2 ng/mL.)
Ephrin B2, Active Protein (Cat# AAA258255)
Full Name
Ephrin B2 Protein, Canine, Recombinant (hFc Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for RPS6KA1/RSK1 activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA1/RSK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)
RPS6KA1/RSK1, Active Protein (Cat# AAA60316)
Full Name
Recombinant RPS6KA1/RSK1 protein
Purity
>=90%
Pricing
Application Data
5'-Nucleotidase, Ecto (NT5E), Active Protein (Cat# AAA148190)
Full Name
Active 5'-Nucleotidase, Ecto (NT5E)
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant chicken TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rgTIMP2 (MW: 23 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rgTIMP2 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant chicken TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161696)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS_PAGE
(Sample: Active recombinant CAPN1, Human)
Calpain 1 (CAPN1), Active Protein (Cat# AAA161456)
Full Name
Active Calpain 1 (CAPN1)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Application Data
TIGIT, Active Protein (Cat# AAA258213)
Full Name
TIGIT Protein, Mouse, Recombinant (mFc Tag)
Gene Names
Tigit; Vstm3
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. 1. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL. 2. Immobilized mouse BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.)
Bcl-XL, Active Protein (Cat# AAA258116)
Full Name
Bcl-XL Protein, Mouse, Recombinant (aa 1-212, His Tag)
Gene Names
Bcl2l1; BclX; Bcl2l; bcl-x; Bcl-XL; Bcl(X)L; bcl2-L-1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). (Conn, G. et al.,1990, Proc. Natl. Acad. Sci. USA 87:1323.) The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.)
FLT1, Active Protein (Cat# AAA257831)
Full Name
FLT1 Protein, Human, Recombinant (His Tag)
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Purity
>90% as determined by SDS-PAGE
Pricing
ELISA
(SARS COV2 RBD B.1.1.529/omicron ACE functional binding ELISA assay. COV2 RBD (coating at 0.1ug-well) binding with Human ACE2-Fc .The linear range was found to be 100 ng/ml)
COVID 19 B.1.1.529 (Omicron) Spike RBD Coronavirus, Active Protein (Cat# AAA76129)
Full Name
COV2 Spike protein RBD Recombinant B.1.1.529 (Omicron)-His
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(Human NRG1/HRG1 (CAT# ATGP3990) is coated at 10 ug/ml (100 ul/well) can bind Human ERBB4. The ED50 range )
ErbB4/Her4, Active Protein (Cat# AAA48406)
Full Name
Recombinant human ErbB4/Her4 protein
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant LBP, Human)
Lipopolysaccharide Binding Protein, Active Protein (Cat# AAA21164)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
LBP; BPIFD2
Applications
Cell Culture, Activity Assay
Purity
>80%
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. Human VGF precursor is 615 amino acids (aa) in length. It contains an 22 aa signal sequence plus a 593 aa mature re)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153074)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of TF-1 cells after stimulated with GM-CSF.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein (Cat# AAA152961)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. It is used for numer)
Trypsin (TRY), Active Protein (Cat# AAA153009)
Full Name
Active Trypsin (TRY)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Besides, Programmed Cell Death Protein 1 (PDCD1) has been identified as an interactor of PDCD1LG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDCD1LG1 and recombinant human PDCD1. Briefly, PDCD1LG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDCD1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PDCD1LG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PDCD1LG1 and PDCD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of PDCD1LG1 with PDCD1.)
Programmed Cell Death Protein 1 Ligand 1, Active Protein (Cat# AAA150101)
Full Name
Active Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
(The dose-effect curve of NRG1 was shown in Figure 2. It was obvious that NRG1 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 5.240 to 9.112 ng/mL.Figure. The dose-effect curve of NRG1 on MCF-7 cells.)
Neuregulin 1, Active Protein (Cat# AAA150117)
Full Name
Active Neuregulin 1 (NRG1)
Gene Names
NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
PTP1B, Active Protein (Cat# AAA107757)
Full Name
PTP1B protein
Gene Names
ptpn1; ptp1b; wu:fk54h03
Applications
SDS-PAGE
Purity
> 95% pure
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200 at 1 ug/ml (100 ul/well) can bind human CD200R1 / Fc Chimera with a linear range of 0.12-16 ng/ml.)
CD200R, Active Protein (Cat# AAA257969)
Full Name
CD200R Protein, Human, Recombinant (His & hFc Tag)
Gene Names
CD200R1; OX2R; MOX2R; CD200R; HCRTR2
Purity
>94% as determined by SDS-PAGE
Pricing
SDS-PAGE
(Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 ug α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 ug MMP-11, (0 hours); 5. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 ug α1-antitrypsin incubated with 1 ug MMP-11 (0 hours); 10. 1.5 yg α1-antitrypsin incubated with 1 ug MMP-11 (24 hours); 11. 1.5 ug α1-antitrypsin (24 hours); 12. Marker.)
MMP-11, Active Protein (Cat# AAA77048)
Full Name
MMP-11 (catalytic domain) (human), (recombinant)
Purity
>95% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Pricing
SDS-PAGE
ACE2, Active Protein (Cat# AAA177000)
Full Name
Recombinant Human ACE2 Protein (Avi-His Tag)
Gene Names
ACE2; ACEH
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
Somatotropin (GH1), Active Protein (Cat# AAA243730)
Full Name
Recombinant Human Somatotropin (GH1) (Active)
Gene Names
GH1; GH; GHN; GH-N; GHB5; IGHD2; hGH-N; IGHD1A; IGHD1B
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target c)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA152974)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of woundsIt can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Ra GAL7 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL7 is 25 ug/mL.)
Galectin 7 (GAL7), Active Protein (Cat# AAA152173)
Full Name
Active Galectin 7 (GAL7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
D-Lactate dehydrogenase, Active Protein (Cat# AAA78979)
Full Name
D-Lactate dehydrogenase, recombinant from bacteria
Pricing
Application Data
(Fig 2: Immobilized Recombinant 2019-nCoV Spike S1-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL.)
COVID 19 Spike S1 Coronavirus, Active Protein (Cat# AAA78533)
Full Name
Recombinant SARS-CoV-2 Spike S1 Protein with His tag and Avi
Applications
Functional Assay
Purity
>95% by SDS-PAGE.
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth. The activity of recombinant human CA3 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA3 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase III, Muscle Specific (CA3), Active Protein (Cat# AAA161899)
Full Name
Active Carbonic Anhydrase III, Muscle Specific (CA3)
Gene Names
CA3; Car3; CAIII
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Netrin 1 (Ntn1) is included in a family of laminin-related secreted proteins. Mouse Ntn1 is a 66-76 kDa glycoprotein that is well known for its involvement in axonal guidance during embryonic development and as an enhancer of cancer cell metastasis. Ntn1 is also involved in outgrowth and migration orientation in the developing CNS and plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas. Slit Homolog 1 (Slit1) can acts as a potent promoter of both Netrin-1 attractive and repulsive activities on distinct neuronal cell types, thereby opening novel perspectives on the role of combinations of cues in brain wiring. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse Ntn1 and recombinant human Slit1. Briefly, Ntn1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to Slit1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Ntn1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse Ntn1 and recombinant human Slit1 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Netrin 1 (Ntn1), Active Protein (Cat# AAA161863)
Full Name
Active Netrin 1 (Ntn1)
Gene Names
Ntn1; AI561871; BC019633; Netrin-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2(BA.4/BA.5) S1+S2 trimer Protein (ECD, His & AVI Tag) (Cat:AAA258581), the EC50 is 2.7-8.1 ng/mL.)
COVID 19 (BA.4/BA.5) Spike S1+S2 trimer Coronavirus, Active Protein (Cat# AAA258581)
Full Name
SARS-CoV-2 (BA.4/BA.5) Spike S1+S2 trimer Protein (ECD, His & AVI Tag), Biotinylated
Purity
>90% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
Pricing
Application Data
(Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25 ng/mL.)
LIGHT, Active Protein (Cat# AAA257879)
Full Name
LIGHT Protein, Human, Recombinant (rFc Tag)
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >5 pmols/min/ug.)
COVID 19 3CLpro/3C-like protease-His Coronavirus, Active Protein (Cat# AAA258110)
Full Name
SARS-CoV-2 (2019-nCoV) 3CLpro/3C-like protease-His Recombinant Protein
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Regenerating Islet Derived Protein 3 Beta (REG3b), Active Protein (Cat# AAA148217)
Full Name
Active Regenerating Islet Derived Protein 3 Beta (REG3b)
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
WB (Western Blot)
(Sample: Recombinant C1qA, Human;Antibody: Rabbit Anti-Human C1qA Ab)
Complement Component 1, Q Subcomponent A (C1qA), Active Protein (Cat# AAA149262)
Full Name
Active Complement Component 1, Q Subcomponent A (C1qA)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant TGFb1, Mouse;Antibody: Rabbit Anti-Mouse TGFb1 Ab)
Transforming Growth Factor Beta 1, Active Protein (Cat# AAA21163)
Full Name
Active Transforming Growth Factor Beta 1 (TGFb1)
Gene Names
Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Reactivity
Mus musculus (Mouse)
Applications
Cell Culture, Activity Assay
Purity
>98%
Pricing
Application Data
FGF-2 (basic), Active Protein (Cat# AAA79309)
Full Name
Rat FGF-2 (basic)
Gene Names
Fgf2; bFGF; Fgf-2
Reactivity
Rat
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Interleukin-32 (IL-32) is an interleukin cytokine usually linked to inflammation. The IL-32 transcript is expressed in various human tissues and organs such as the spleen, thymus, leukocyte, lung, small intestine, colon, prostate, heart, placenta, liver, muscle, kidney, pancreas, and brain. Cytokines are critical components of cell signaling pathways that are involved in the regulation of cell growth, metabolism, hormone signaling, immune regulation and a variety of other physiological functions. FGFR1 has been identified as an interactor of IL-32, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL-32 and recombinant human FGFR1. Briefly, IL-32 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL-32 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IL-32 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.15 ug/mL.)
Interleukin 32 (IL32), Active Protein (Cat# AAA161861)
Full Name
Active Interleukin 32 (IL32)
Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Telomerase reverse transcriptase (TERT) is a subunit of the enzyme complex telomerase, which adds nucleotides to the ends of telomeres as they become shortened during cell division .Telomerase complex plays a key role in cancer formation by telomere dependent or independent mechanisms. According to statistics, human telomerase reverse transcriptase (h-TERT) is overexpressed in more than 85% of tumors with diverse histologies, with little expression in normal tissues. Expression of h-TERT correlates with activity of telomerase, which is required for the capacity for limitless replication, a hallmark of cancer. The X-Ray Repair Cross Complementing 6 (XRCC6) is high affinity receptor for TERT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TERT and recombinant rat XRCC6. Briefly, TERT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to XRCC6-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TERT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant TERT and recombinant rat XRCC6 was shown in Figure 1, the EC50 for this effect is 0.009 ug/mL.)
Telomerase Reverse Transcriptase (TERT), Active Protein (Cat# AAA161880)
Full Name
Active Telomerase Reverse Transcriptase (TERT)
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant mouse GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant mouse GSTa3 is >60,000 pmol/min/ug.)
Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161767)
Full Name
Active Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta3; Gst2-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Spike glycoprotein (S), Active Protein (Cat# AAA244033)
Full Name
Recombinant Human Novel Coronavirus Spike glycoprotein (S), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
(Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 4-16 ng/mL.)
IL28B, Active Protein (Cat# AAA257997)
Full Name
IL28B Protein, Human, Recombinant (His Tag)
Gene Names
IFNL3; IL28B; IL28C; IL-28B
Purity
>96% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (BA.4/BA.5) Spike S1 Protein (His Tag)(Cat:AAA258579), the EC50 is 30-90ng/mL.)
COVID 19 (BA.4/BA.5) Spike S1 Coronavirus, Active Protein (Cat# AAA258579)
Full Name
SARS-CoV-2 (BA.4/BA.5) Spike S1 Protein (His Tag)
Purity
>95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
Pricing
Application Data
(CyaA accelerates cell detachment and migration of TLR-activated DCs.Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.)
MIP-3 beta (CCL19), Active Protein (Cat# AAA76385)
Full Name
Mouse MIP-3 beta (CCL19) Recombinant
Gene Names
Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
Purity
>98%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
VEGF121, Active Protein (Cat# AAA79142)
Full Name
Human VEGF121
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
SDS-PAGE
(SDS-PAGE analysis of recombinant human Galectin-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
Galectin-1, Active Protein (Cat# AAA79150)
Full Name
Human Galectin-1
Gene Names
LGALS1; GBP; GAL1
Reactivity
Human
Purity
>98% by SDS-PAGE & Coomassie stain
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.