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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2200-2250 of 2875 product results
Application Data
(Measured by its ability to bind human CD32a-His in a functional ELISA.)
IgG2, Active Protein (Cat# AAA258022)
Full Name
IgG2 Protein, Human, Recombinant (257 Ser/Ala)
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1),the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL.)
SARS-CoV Spike/S1, Active Protein (Cat# AAA258071)
Full Name
SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)
Reactivity
SARS
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His (40589-V08B1) is 400-1200 ng/mL.)
COVID 19 Spike S1+S2 ECD-His Coronavirus, Active Protein (Cat# AAA258075)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(Epithelial Neutrophil Activating Peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines. Full-length ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. ENA-78 is expressed by many immune cells, such as macrop)
Epithelial Neutrophil Activating Peptide 78 (ENA78), Active Protein (Cat# AAA153064)
Full Name
Active Epithelial Neutrophil Activating Peptide 78 (ENA78)
Gene Names
Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and he)
Hedgehog Homolog, Sonic (SHH), Active Protein (Cat# AAA153105)
Full Name
Active Hedgehog Homolog, Sonic (SHH)
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Application Data
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA148152)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
SDS-PAGE
Cyclophilin F, Active Protein (Cat# AAA48516)
Full Name
Cyclophilin F (PPIF), 30-207aa, Human,His tag, E Coli (Bioactivity Validated)
Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Applications
SDS-PAGE
Purity
> 95% by SDS PAGE
Pricing
Bioactivity
MERS-CoV Spike/S1 Protein, Active Protein (Cat# AAA177991)
Full Name
Recombinant MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with FBLN2.)
Fibulin 2 (FBLN2), Active Protein (Cat# AAA161909)
Full Name
Active Fibulin 2 (FBLN2)
Gene Names
Fbln2; FIBL-2; 5730577E14Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag) (40150-V05H1), the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag)is 50-120 ng/mL.)
SARS-CoV Spike/S1, Active Protein (Cat# AAA258070)
Full Name
SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag)
Reactivity
SARS
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153042)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
SARS-CoV Spike S1+S2 ECD-His (S577A, Isolate Tor2), Active Protein (Cat# AAA177995)
Full Name
Recombinant SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IP-10 (CXCL10), Active Protein (Cat# AAA23887)
Full Name
Recombinant Human IP-10 (CXCL10)
Gene Names
CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
Purity
>95% as determined by SDS-PAGE
Pricing
Adenylate Kinase 2, Active Protein (Cat# AAA38616)
Full Name
Recombinant Human Adenylate Kinase 2
Gene Names
AK2; ADK2; AK 2
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
Bioactivity
(Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant pig CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant pig CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161784)
Full Name
Active C Reactive Protein (CRP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. 1. Immobilized mouse EphA2 at 2 ug/ml (100 ul/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml. 2. Immobilized mouse EphA2 at 2 ug/ml (100 ul/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/ml.)
EphA2, Active Protein (Cat# AAA258177)
Full Name
EphA2 Protein, Mouse, Recombinant (His Tag)
Gene Names
Epha2; Eck; Myk2; Sek2; Sek-2; AW545284
Purity
>98% as determined by SDS-PAGE
Pricing
Bioactivity
(Human IL-1ra/IL-1F3 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ?10 ng/ml.)
IL-1RA/IL-1F3, Active Protein (Cat# AAA48355)
Full Name
Recombinant human IL-1RA/IL-1F3 protein
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS - PAGE
Pricing
Bioactivity
(Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable y)
Catalase (CAT), Active Protein (Cat# AAA153115)
Full Name
Active Catalase (CAT)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44 66 kDa, variably N and O glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153097)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Transforming growth factor beta 1 (TGF-beta1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation)
Transforming Growth Factor Beta 1 (TGFb1), Active Protein (Cat# AAA152981)
Full Name
Active Transforming Growth Factor Beta 1 (TGFb1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL8 in V- bottom shaped 96-well microtiter plate.)
Galectin 8 (GAL8), Active Protein (Cat# AAA153023)
Full Name
Active Galectin 8 (GAL8)
Gene Names
Lgals8; Lgals-8; AI326142; D13Ertd524e; 1200015E08Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Tumor necrosis factor receptor superfamily member 10B protein (TNFRSF10B), Active Protein (Cat# AAA114240)
Full Name
Recombinant Human Tumor necrosis factor receptor superfamily member 10B protein (TNFRSF10B) (Active)
Gene Names
TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE
Platelet factor 4 protein (Pf4), Active Protein (Cat# AAA114234)
Full Name
Recombinant Mouse Platelet factor 4 protein (Pf4) (Active)
Gene Names
Pf4; Cxcl4; Scyb4
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3, Active Protein (Cat# AAA177934)
Full Name
Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-mFC)
Gene Names
SIGLEC15; CD33L3; HsT1361; SIGLEC-15
Purity
>90% as determined by reducing SDS-PAGE
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant PK2, Human;Antibody: Rabbit Anti-Human PK2 Ab)
Prokineticin 2 (PK2), Active Protein (Cat# AAA149227)
Full Name
Active Prokineticin 2 (PK2)
Gene Names
PROK2; BV8; HH4; PK2; KAL4; MIT1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Sequence
Galectin 8 (GAL8), Active Protein (Cat# AAA152175)
Full Name
Active Galectin 8 (GAL8)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(The standard curve of 4-Nitrophenol.Carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50uL of various concentrations of CES1 (dilute by assay buffer) to 50uL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400nm.One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 1396 pmol/min/ug.Specific Activity (pmol/min/ug) = ΔOD*F/T*NΔOD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T=TimeN=Amount of enzyme)
Carboxylesterase 1 (CES1), Active Protein (Cat# AAA149260)
Full Name
Active Carboxylesterase 1 (CES1)
Gene Names
CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
(Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.82 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161781)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; APCS
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Cubilin (CUBN) is a 460 kDa intrinsic factor-vitamin B12 receptor protein and it is expressed in kidney and small intestine. CUBN is a component of a multiligand endocytic receptor complex, which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Besides CUBN serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac and it plays an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CUBN and recombinant mouse APOA1. Briefly, CUBN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOA1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CUBN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CUBN and recombinant mouse APOA1 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Cubilin (CUBN), Active Protein (Cat# AAA161888)
Full Name
Active Cubilin (CUBN)
Gene Names
CUBN; IFCR; MGA1; gp280
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.4-2 ug/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt-3a.)
SFRP1, Active Protein (Cat# AAA258118)
Full Name
SFRP1 Protein, Mouse, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
SPARC, Active Protein (Cat# AAA258167)
Full Name
SPARC Protein, Mouse, Recombinant (His Tag)
Gene Names
Sparc; ON; BM-40
Purity
>96% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. 1. Immobilized human EphA7 at 2 ug/ml (100 ul/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml. 2. Immobilized mouse EphA7 at 2 ug/ml (100 ul/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml.)
Ephrin A4, Active Protein (Cat# AAA258180)
Full Name
Ephrin A4 Protein, Mouse, Recombinant (hFc Tag)
Purity
>92% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human APOH-his at 2 ug/mL (100 ul/well) can bind biotinylated mouse LDLR-his, The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.)
Apolipoprotein H, Active Protein (Cat# AAA257970)
Full Name
Apolipoprotein H Protein, Human, Recombinant (His Tag)
Gene Names
APOH; BG; B2G1; B2GP1
Purity
>96% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EPHA3-His at 10 ug/ml (100 ul/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6.2-14.6 ng/ml.)
EphA3, Active Protein (Cat# AAA257975)
Full Name
EphA3 Protein, Human, Recombinant (His Tag)
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human human B7-H2 at 1 ug/ml (100 ul/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.)
ICOS, Active Protein (Cat# AAA257874)
Full Name
ICOS Protein, Human, Recombinant (His & hFc Tag)
Gene Names
ICOS; AILIM; CD278; CVID1
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.4-1.6 ug/mL.)
FGF8, Active Protein (Cat# AAA258053)
Full Name
FGF8 Protein, Human, Recombinant
Gene Names
FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(HTRF assay for NEK7 activity 1 uM STK S3 substrate was incubated with different concentrations of NEK7 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ACE2, Active Protein (Cat# AAA177001)
Full Name
Recombinant Human ACE2 Protein (mFc Tag)
Gene Names
ACE2; ACEH
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FS)
Luteinizing Hormone (LH), Active Protein (Cat# AAA153035)
Full Name
Active Luteinizing Hormone (LH)
Gene Names
UCP1; UCP; SLC25A7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS_PAGE
(Sample: Active recombinant SOD1, Human)
Superoxide Dismutase 1 (SOD1), Active Protein (Cat# AAA153106)
Full Name
Active Superoxide Dismutase 1 (SOD1)
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
B7 Homolog 4/B7-H4/VTCN1 Biotinylated, Active Protein (Cat# AAA177958)
Full Name
Recombinant Human B7 Homolog 4/B7-H4/VTCN1 (C-Fc-Avi) Biotinylated
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF23, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF23 and recombinant human FGFR1. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FGF23 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.17 ug/mL.)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA161772)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Transforming Growth Factor-beta Receptor Type II, Active Protein (Cat# AAA174598)
Full Name
Recombinant Human Transforming Growth Factor-beta Receptor Type II Protein
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TBRII; TBR-ii; TGFR-2; TGFbeta-RII
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Glutathione S-Transferase (GST), Active Protein (Cat# AAA48483)
Full Name
Glutathione S-Transferase (GST), 1-218 aa, Schistosoma japonicum E Coli (Bioactivity Validated)
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. Human VGF precursor is 615 amino acids (aa) in length. It contains an 22 aa signal sequence plus a 593 aa mature re)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153075)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
VEGF-E, Active Protein (Cat# AAA79204)
Full Name
Orf Virus VEGF-E
Reactivity
Orf virus
Purity
> 90% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Human CTLA-4 inhibits Human B7 1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range )
CTLA-4, Active Protein (Cat# AAA48323)
Full Name
Recombinant CTLA-4
Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Applications
Bioactivity, SDS-PAGE
Purity
> 95% by SDS - PAGE
Pricing
Bioactivity
(Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor. It is primarily secreted by macrophages and monocytes and can form disulfide-linked multimers. Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP. It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides. It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance. A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion. It also inhibits proteasome activity in activated endothelial cells. The activity of recombinant human PAI-2 was measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) in the assay buffer 50 mM Tris, 0.01% Tween 20, pH 8.5. The 50 ul different concentrations of rhPAI-2 (MW: 48.2 KD) was incubated with 50ul 2ug/ml rhuPA (EPA140Mu61) at room temperature for 15 minutes. Loading 50 uL of the incubated mixtures into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate (Z-GGR-AMC). Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human PAI2 significantly decreased uPA activity. The inhibition IC50 was )
Plasminogen Activator Inhibitor 2 (PAI2), Active Protein (Cat# AAA161739)
Full Name
Active Plasminogen Activator Inhibitor 2 (PAI2)
Gene Names
SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 1, Q Subcomponent A (C1qA) is a protein that plays a crucial role in the innate immune system. It is part of the C1 complex, which is the first component of the classical complement pathway. The C1 complex is composed of one molecule each of C1q, C1r, and C1s. C1q is the recognition subcomponent of the complex and and initiating the complement cascade. C1qA plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. It is reported that C1qA can bind to the C-terminal of CALR through its gelatin-as-region, and this interaction may play an important role in regulating complement activation and cell signaling. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C1qA and recombinant human CALR. Briefly, C1qA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse C1qA and recombinant human CALR was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Complement Component 1, Q Subcomponent A (C1qA), Active Protein (Cat# AAA161911)
Full Name
Active Complement Component 1, Q Subcomponent A (C1qA)
Gene Names
C1qa; C1q; AI255395
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 60-300 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.)
ALK-1, Active Protein (Cat# AAA258143)
Full Name
ALK-1 Protein, Mouse, Recombinant (His & hFc Tag)
Gene Names
Acvrl1; Alk1; Acvrlk1; AI115505; AI427544
Purity
>95% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.