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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2150-2200 of 2583 product results
Bioactivity
(Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors. Human growth hormone is a 191 amino acid single-chain polypeptide produced via the anterior pituitary of the brain in the acidophilic, somatotrophic cells. Its production is tightly regulated through several complex feedback mechanisms in response to stress, exercise, nutrition, sleep, and growth hormone itself. Annexin A2 (ANXA2) has been shown to play multiple roles in growth, development, metabolism and ANXA2 is a high affinity receptor for GH. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GH and recombinant human ANXA2. Briefly, GH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ANXA2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GH and recombinant human ANXA2 was shown in Figure 1, the EC50 for this effect is 2.1 ug/mL.)
Growth Hormone (GH), Active Protein (Cat# AAA161667)
Full Name
Active Growth Hormone (GH)
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of sixIGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structuresand bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Withintissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and blocktheir access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.IGFBP3 also interacts with cell-surface proteins, affecting cell signaling fromoutside the cell or after internalization, and also enters the cell nucleus where itbinds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)has been identified as an interactor of IGFBP3, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human IGFBP3 andrecombinant human FN.Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100ul were then transferred to FN-coated microtiter wells andincubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1hwith anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78ng/ml.)
Insulin Like Growth Factor Binding Protein 3 (IGFBP3), Active Protein (Cat# AAA161673)
Full Name
Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
Gene Names
IGFBP3; IBP3; BP-53
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 alpha (IL-1alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1alphais produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. To test the effect of IL-1alpha on cell apoptosis, MCF7 cells were seeded into 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant goat IL-1alpha. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37 degree C. Proliferation of MCF7 cells after incubation with IL-1alpha for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant goat IL-1alpha for 72h. The result was shown in Figure 2. It was obvious that IL-1alpha significantly inhibit cell viability of MCF7cells. The ED50 is 2.13 ug/ml.)
Interleukin 1 Alpha (IL1a), Active Protein (Cat# AAA161676)
Full Name
Active Interleukin 1 Alpha (IL1a)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(IL1alpha(Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1alpha results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1alpha. MCF-7 cells were seeded overnight at a density of 4,000 cells/well, and then treated with or without various concentrations of IL1alpha for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours in at 37 degree C. Inhibition of MCF-7 cell proliferation after incubation with IL1alpha for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1alpha for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1alpha significantly decreased cell viability of MCF-7 cells and the EC50 was 4.69 ug/ml.)
Interleukin 1 Alpha (IL1a), Active Protein (Cat# AAA161677)
Full Name
Active Interleukin 1 Alpha (IL1a)
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of recombinant human IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.)
Interleukin 5 (IL5), Active Protein (Cat# AAA161678)
Full Name
Active Interleukin 5 (IL5)
Gene Names
IL5; EDF; TRF; IL-5
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. The activity of LIF is usually measured by a cell proliferation assay using TF-1 cells. TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant human LIF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with LIF for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with rhLIF for 3 days. The result was shown in Figure 2. It was obvious that rhLIF significantly increased cell viability of TF-1 cells. The ED50 is 1.86 ng/ml.)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA161682)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant bovine MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rbMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 degree C for 2 hours. The activated rbMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rbMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rbMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant bovine MMP1 is > 150 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA161684)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Gene Names
MMP1; MMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant sheep MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rsMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 degree C for 2 hours. The activated rsMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rsMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rsMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant sheep MMP1 is > 40 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA161685)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant pig MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rpMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 degree C for 2 hours. The activated rpMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rpMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rpMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant pig MMP1 is > 10 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA161686)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Gene Names
MMP1; Mmp1a
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.)
Nerve Growth Factor (NGF), Active Protein (Cat# AAA161688)
Full Name
Active Nerve Growth Factor (NGF)
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. The activity of recombinant bovine TIMP3 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rbTIMP3 (MW: 23.25 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rbTIMP3 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant bovine TIMP3 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 3 (TIMP3), Active Protein (Cat# AAA161700)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)
Gene Names
TIMP3; TIMP-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 3a (C3a) is a component of the complement system, this protein is also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. It's been identified that C3A can interact with Complement Factor I (CFI), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C3a and recombinant human CFI. Briefly, C3a was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CFI-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C3a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C3a and recombinant human CFI was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Complement Component 3a (C3a), Active Protein (Cat# AAA161726)
Full Name
Active Complement Component 3a (C3a)
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation. It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The activity of recombinant human TFPI was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 20 ul different concentrations of recombinant human TFPI (MW: 55.58 KD) was incubated with 20 ul diluted trypsin at 37 degree C for 15 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 20 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TFPI significantly decreased trypsin activity. The inhibition IC50 was )
Tissue Factor Pathway Inhibitor (TFPI), Active Protein (Cat# AAA161731)
Full Name
Active Tissue Factor Pathway Inhibitor (TFPI)
Gene Names
TFPI; EPI; TFI; LACI; TFPI1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant EMILIN1, Human)
Elastin Microfibril Interface Located Protein 1 (EMILIN1), Active Protein (Cat# AAA161733)
Full Name
Active Elastin Microfibril Interface Located Protein 1 (EMILIN1)
Gene Names
EMILIN1; EMI; gp115; EMILIN
Reactivity
Homo sapiens (Human)
Applications
Activity Assay
Purity
>80%
Pricing
Bioactivity
(Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein's specific biological action and is responsible for the interaction with its receptor. LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol. A functional binding ELISA assay was conducted to detect the interaction of recombinant human CGa/LHb and recombinant human Dopamine Receptor D1 (DRD1). Briefly, biotin-linked CGa/LHb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to DRD1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of CGa/LHb and DRD1 was shown in Figure 1, the EC50 for this effect is 0.644 ug/mL.)
Luteinizing Hormone (LH), Active Protein (Cat# AAA161734)
Full Name
Active Luteinizing Hormone (LH)
Gene Names
CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor. It is primarily secreted by macrophages and monocytes and can form disulfide-linked multimers. Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP. It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides. It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance. A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion. It also inhibits proteasome activity in activated endothelial cells. The activity of recombinant mouse PAI-2 was measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) in the assay buffer 50 mM Tris, 0.01% Tween 20, pH 8.5. The 50 ul different concentrations of rmPAI-2 (MW: 47.9 KD) was incubated with 50ul 2ug/ml rhuPA (EPA140Mu61) at room temperature for 15 minutes. Loading 50 uL of the incubated mixtures into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate (Z-GGR-AMC). Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that PAI2 significantly decreased uPA activity. The inhibition IC50 was )
Plasminogen Activator Inhibitor 2 (PAI2), Active Protein (Cat# AAA161740)
Full Name
Active Plasminogen Activator Inhibitor 2 (PAI2)
Gene Names
Serpinb2; PAI-2; Planh2; ovalbumin
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast Growth Factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, ce)
Fibroblast Growth Factor 9 (FGF9), Active Protein (Cat# AAA152957)
Full Name
Active Fibroblast Growth Factor 9 (FGF9)
Gene Names
Fgf9; Eks
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-3 on TF-1 cells)
Interleukin 3 (IL3), Active Protein (Cat# AAA152968)
Full Name
Active Interleukin 3 (IL3)
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of HepG2 cells proliferation after stimulated with TGF-beta1.)
Transforming Growth Factor Beta 1 (TGFb1), Active Protein (Cat# AAA152982)
Full Name
Active Transforming Growth Factor Beta 1 (TGFb1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152991)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFa
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Neutrophil elastase (NE), also known as polymorphonuclear leukocyte elastase, is a major protease in the primary granules of neutrophils, is involved in microbicidal activity. It is located primarily in the azurophil granules of polymorphonuclear leukocyt)
Neutrophil Elastase (NE), Active Protein (Cat# AAA153000)
Full Name
Active Neutrophil Elastase (NE)
Gene Names
Elane; NE; Ela2; F430011M15Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Myeloid cell surface antigen CD33 (CD33), Active Protein (Cat# AAA114015)
Full Name
Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE
C-C motif chemokine 3 protein (Ccl3), Active Protein (Cat# AAA114236)
Full Name
Recombinant Rat C-C motif chemokine 3 protein (Ccl3) (Active)
Gene Names
Ccl3; Scya3; MIP-1a
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE
Fibroblast growth factor 23 protein (FGF23), Active Protein (Cat# AAA114242)
Full Name
Recombinant Human Fibroblast growth factor 23 protein (FGF23) (Active)
Gene Names
FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Siglec-10, Active Protein (Cat# AAA177947)
Full Name
Recombinant Human Siglec-10 (C-Fc)
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
CD28/TP44 Biotinylated, Active Protein (Cat# AAA177952)
Full Name
Recombinant Human/Cynomolyus CD28/TP44 (C-Fc-Avi) Biotinylated
Gene Names
CD28; Tp44
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3, Active Protein (Cat# AAA177967)
Full Name
Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
Bioactivity
SARS-CoV Spike Protein, Active Protein (Cat# AAA177982)
Full Name
Recombinant SARS-CoV Spike Protein (RBD, mFc Tag)(Active)
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
MERS-CoV Spike Protein, Active Protein (Cat# AAA177989)
Full Name
Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)
Purity
>85% as determined by SDS-PAGE
Pricing
Insulin growth factor-like family member 1 (IGFL1), Active Protein (Cat# AAA116918)
Full Name
Recombinant Human Insulin growth factor-like family member 1 (IGFL1)
Gene Names
IGFL1; UNQ644; APRG644
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE
T-cell immunoreceptor with Ig and ITIM domains, Active Protein (Cat# AAA117496)
Full Name
Recombinant Human T-cell immunoreceptor with Ig and ITIM domains
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
SDS-PAGE
Kallikrein 7, Active Protein (Cat# AAA174597)
Full Name
Recombinant Human Kallikrein 7/KLK7 Protein (His Tag)
Gene Names
KLK7; hK7; SCCE; PRSS6
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Transforming Growth Factor-beta Receptor Type II, Active Protein (Cat# AAA174598)
Full Name
Recombinant Human Transforming Growth Factor-beta Receptor Type II Protein
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TBRII; TBR-ii; TGFR-2; TGFbeta-RII
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
(> 95 % as determined by reducing SDS-PAGE.)
S100 Calcium Binding Protein B, Active Protein (Cat# AAA174599)
Full Name
Recombinant Human S100 Calcium Binding Protein B Protein
Gene Names
S100B; NEF; S100; S100-B; S100beta
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Carnosine Dipeptidase 1, Active Protein (Cat# AAA174600)
Full Name
Recombinant Human Carnosine Dipeptidase 1 Protein
Gene Names
CNDP1; CN1; CPGL2; HsT2308
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Cathepsin S, Active Protein (Cat# AAA174601)
Full Name
Recombinant Mouse Cathepsin S Protein
Gene Names
Ctss; Cats
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
(SDS-PAGE)
TrkB/NTRK2, Active Protein (Cat# AAA174698)
Full Name
Recombinant Human TrkB/NTRK2 Protein (His Tag)(Active)
Gene Names
NTRK2; OBHD; TRKB; trk-B; EIEE58; GP145-TrkB
Applications
SDS-PAGE
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
HLA Class II Histocompatibility Antigen gamma Chain, Active Protein (Cat# AAA174708)
Full Name
Recombinant Mouse HLA Class II Histocompatibility Antigen gamma Chain Protein
Gene Names
CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
CD16a / FCGR3A, Active Protein (Cat# AAA173505)
Full Name
Recombinant Human CD16a / FCGR3A Protein (176 Val, His & AVI tag)
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Purity
> 90 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
B7-H6 / B7H6, Active Protein (Cat# AAA173515)
Full Name
Recombinant Human B7-H6 Protein (His Tag)(Active)
Gene Names
NCR3LG1; B7H6; B7-H6; DKFZp686O24166
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
DMBT1 / Muclin, Active Protein (Cat# AAA173542)
Full Name
Recombinant Human DMBT1 / Muclin Protein (His tag)
Gene Names
DMBT1; SAG; GP340; SALSA; muclin
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
cIAP1 / HIAP2, Active Protein (Cat# AAA173550)
Full Name
Recombinant Human cIAP1 / HIAP2 Protein (AVI tag)
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Purity
> 92 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
FGFR2, Active Protein (Cat# AAA173557)
Full Name
Recombinant Human FGFR2 Protein (His & Fc tag)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Purity
> 90 % as determined by SDS-PAGE
Pricing
Application Data
(SDS-PAGE)
PDGFRa / CD140a, Active Protein (Cat# AAA173562)
Full Name
Recombinant Human PDGFRa / CD140a Protein (His tag)
Gene Names
PDGFRA; CD140A; PDGFR2; PDGFR-2
Purity
> 97 % as determined by SDS-PAGE
Pricing
LDLR / LDL Receptor, Active Protein (Cat# AAA173570)
Full Name
Recombinant Human LDLR Protein (His Tag)(Active)
Gene Names
LDLR; FH; FHC; LDLCQ2
Purity
> 85 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
Transferrin / CD176 / Serotransferrin, Active Protein (Cat# AAA173584)
Full Name
Recombinant Mouse Transferrin / CD176 / Serotransferrin Protein (Fc tag)
Gene Names
Trf; HP; Tf; Tfn; hpx; Cd176; AI266983
Purity
>90 % as determined by SDS-PAGE
Pricing
SDS-PAGE
SHP2 / PTPN11, Active Protein (Cat# AAA173592)
Full Name
Recombinant Mouse SHP2 / PTPN11 Protein (His tag)
Gene Names
Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
Purity
> 90 % as determined by SDS-PAGE
Pricing
Programmed cell death 1 ligand 1 (CD274), Active Protein (Cat# AAA233604)
Full Name
Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial (Active)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Syndecan-2 (SDC2), Active Protein (Cat# AAA235618)
Full Name
Recombinant Human Syndecan-2 (SDC2), Partial
Gene Names
SDC2; HSPG; CD362; HSPG1; SYND2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
Fibronectin Type III Domain Containing Protein 5 (FNDC5), Active Protein (Cat# AAA146626)
Full Name
Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Gene Names
FNDC5; FRCP2; irisin
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.