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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2400-2450 of 2567 product results
Bioactivity
(IFNgamma R2 (Interferon gamma receptor 2; also called IFN gamma R beta IFN gamma RII, or AF1) is a 6064 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecule). It is highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. IFNgammaR2 is a cell-surface receptor that is required for interferon-gamma signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. IFNgR2 have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFNgamma, the complex contains two each of IFNgamma R1, R2 and Jak1 molecules. Binding of IFNgamma to IFNgamma R1 recruits Jak2 to IFN-gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis. A functional binding ELISA assay was conducted to detect the interaction of recombinant human IFNgR2 and recombinant rat JAK2. Briefly, biotin-linked IFNgR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of IFNgR2 and JAK2 was shown in Figure 1, the EC50 for this effect is 1.02 ug/mL.)
Interferon Gamma Receptor 2 (IFNgR2), Active Protein (Cat# AAA161936)
Full Name
Active Interferon Gamma Receptor 2 (IFNgR2)
Gene Names
IFNGR2; AF-1; IFGR2; IFNGT1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), also known as DT-diaphorase, is a widely-distributed FAD-dependent flavoprotein that promotes 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes. As a result it prevents the one electron reduction of quinones that results in the production of radical species. NQO1 is a highly-inducible enzyme that is regulated by the Keap1/Nrf2/ARE pathway. The increase and decrease of NQO1 levels are associated with decreased and increased susceptibilities to oxidative stress, respectively. Thus, NQO1 is a marker cytoprotective enzyme in oxidative stress. Independently of its catalytic function, NQO1 plays a role in regulating the proteosomal degradation of p53, p73a, and p33. NQO1 physically interacts with p53 and p73 in an NADH-dependent manner and protects them from 20S proteasomal degradation in a ubiquitin independent pathway. The activity assay of recombinant rat NQO1 was measured by its ability to oxidize the substrate resazurin to resorufin. The rrNQO1 was diluted to 0.05 ug/ml in the assay buffer 50 mM HEPES, 0.2 M NaCl, 5 uM FAD, 0.05% Tween 20, pH 7.5. 50 ul 0.05 ug/ml rhNQO1 was added into the microplate and start the reaction by adding 50 ul substrate mixture of 400 uM beta-NADH and 20 uM resazurin which was diluted in assay buffer. Read at excitation and emission wavelengths of 540 nm and 585 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant rat NQO1 is >45000 pmol/min/ug.)
NADH Dehydrogenase, Quinone 1 (NQO1), Active Protein (Cat# AAA161939)
Full Name
Active NADH Dehydrogenase, Quinone 1 (NQO1)
Gene Names
Nqo1; Dia4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Uracil Phosphoribosyltransferase (UPRT) is an important enzyme involved in the pyrimidine salvage pathway, which is a critical metabolic pathway for the synthesis of nucleotides in various organisms, including humans. This enzyme plays a key role in the conversion of uracil, a pyrimidine base, into uridine-5'-phosphate (UMP), a precursor for the synthesis of RNA and DNA. Besides, Purine Nucleoside Phosphorylase (PNP) has been identified as an interactor of UPRT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human UPRT and recombinant mouse PNP. Briefly, UPRT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PNP-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-UPRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human UPRT and recombinant mouse PNP was shown in Figure 1, the EC50 for this effect is 0.017 ug/mL.)
Uracil Phosphoribosyltransferase (UPRT), Active Protein (Cat# AAA161941)
Full Name
Active Uracil Phosphoribosyltransferase (UPRT)
Gene Names
UPRT; UPP; FUR1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells.The ED50 for this effect is typically 2.9 ng/ml.)
Stem Cell Factor (SCF), Active Protein (Cat# AAA161694)
Full Name
Active Stem Cell Factor (SCF)
Gene Names
Kitlg; Mgf; SCF; Kitl
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant human TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rhTIMP2 (MW: 51.75 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rhTIMP2 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161698)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Gene Names
TIMP2; DDC8; CSC-21K
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant rat TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rrTIMP2 (MW: 22.98 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rrTIMP2 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161699)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tissue Inhibitors of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. The activity of recombinant rat TIMP4 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rrTIMP4 (MW: 22.79 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rrTIMP4 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TIMP4 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), Active Protein (Cat# AAA161703)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Collagen Type IV Alpha 1 (COL4a1), a secreted glycoprotein,is a member of the type IV collagen family. COL4a1 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. It can inhibit angiogenesis and tumor formation, and specifically inhibit endothelial cell proliferation, migration and tubule formation. COL4A1 mutations can remain asymptomatic or cause devastating disease. Neonates and children may present with porencephaly, intracerebral hemorrhage, or hemiparesis, whereas adults tend to develop intracranial aneurysms or retinal arteriolar tortuosities. Glycoprotein VI (GP6) has been identified as an interactor of COL4a1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human COL4a1 and recombinant human GP6. Briefly, COL4a1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GP6-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-COL4a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human COL4a1 and recombinant human GP6 was shown in Figure 1, the EC50 for this effect is 0.05 ug/mL.)
Collagen Type IV Alpha 1 (COL4a1), Active Protein (Cat# AAA161709)
Full Name
Active Collagen Type IV Alpha 1 (COL4a1)
Gene Names
COL4A1; ICH; HANAC; POREN1; arresten
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Carcinoembryonic antigen (CEA), as one of the common tumor markers, is a human glycoprotein involved in cell adhesion and is expressed during human fetal development. Since the birth of human, CEA expression is largely inhibited, with only low levels in the plasma of healthy adults. Generally, CEA will overexpressed in many cancers, including gastric, breast, ovarian, lung, and pancreatic cancers, especially colorectal cancer. The lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is also a secreted, which was first identified as cancer and metastasis associated protein. LGALS3BP has been identified as an interactor of CEA, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CEA and recombinant human LGALS3BP. Briefly, biotin-linked CEA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LGALS3BP-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of CEA and LGALS3BP was shown in Figure 1, and this effect was in a dose dependent manner.)
Carcinoembryonic Antigen (CEA), Active Protein (Cat# AAA161710)
Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Bone Morphogenetic Protein Receptor 2 (BMPR2) is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. BMPR2 is a TGFbeta type II receptor expressed on cumulus cells. Growth Differentiation Factor 9 (GDF9) can bind to BMPR2 through different type I receptors, thereby regulating downstream metabolic reactions. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human BMPR2 and recombinant human GDF9. Briefly, biotin-linked BMPR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GDF9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human BMPR2 and recombinant human GDF9 was shown in Figure 1, the EC50 for this effect is 42.87 ng/mL.)
Bone Morphogenetic Protein Receptor 2 (BMPR2), Active Protein (Cat# AAA161656)
Full Name
Active Bone Morphogenetic Protein Receptor 2 (BMPR2)
Gene Names
BMPR2; BMR2; PPH1; BMPR3; BRK-3; T-ALK; BMPR-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 16 (IL-16), formerly known as lymphocyte chemoattractant factor or LCF, is a pro-inflammatory cytokine that is chemotactic for CD4 T lymphocytes, monocytes, and eosinophils. In addition to inducing chemotaxis, IL-16 can upregulate IL-2 receptor and HLA-DR4 expression, inhibit T cell receptor (TcR)/CD3-dependent activation, and promote repression of HIV-1 transcription.Besides,Interleukin 1 Alpha (IL1a) has been identified as an interactor of IL-16, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IL-16 and recombinant dog IL1a. Briefly, IL-16 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL1a-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL-16 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse IL-16 and recombinant dog IL1a was shown in Figure 1, the EC50 for this effect is 1.9 ug/mL.)
Interleukin 16 (IL16), Active Protein (Cat# AAA161675)
Full Name
Active Interleukin 16 (IL16)
Gene Names
Il16; mKIAA4048
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant human IL6. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with rhIL6 for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human IL6 for 3 days. The result was shown in Figure 2. It was obvious that recombinant human IL6 significantly increased cell viability of TF-1 cells, the EC50 was 0.016 ug/ml.)
Interleukin 6 (IL6), Active Protein (Cat# AAA161680)
Full Name
Active Interleukin 6 (IL6)
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA161683)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
CSF1; MCSF; CSF-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells.The ED50 for this effect is typically 0.54 ng/ml.)
Stem Cell Factor (SCF), Active Protein (Cat# AAA161692)
Full Name
Active Stem Cell Factor (SCF)
Gene Names
KITLG; CSF; MGF; 710-712
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44 66 kDa, variably N and O glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153097)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44 66 kDa, variably N and O glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153099)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerizati)
Thymosin Beta 4 (TMSB4X), Active Protein (Cat# AAA153102)
Full Name
Active Thymosin Beta 4 (TMSB4X)
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), is a divergent member of the Transforming Growth Factor beta (TGF-beta ) superfamily. Human GDF-15 shares 66% and 68% amino acid sequence identity with the ra)
Growth Differentiation Factor 15 (GDF15), Active Protein (Cat# AAA153111)
Full Name
Active Growth Differentiation Factor 15 (GDF15)
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 21 (FGF21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family which possess broad mitogenic and cell survival activities, and are in)
Fibroblast Growth Factor 21 (FGF21), Active Protein (Cat# AAA153123)
Full Name
Active Fibroblast Growth Factor 21 (FGF21)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 37, also known as Interleukin 1 Zeta (IL1z), is an anti-inflammatory cytokine. Interleukines are cytokines that make an important part of immune signaling. It belongs to the interleukin-1 family. IL-37 plays a role in protecting the body again)
Interleukin 1 Zeta (IL1z), Active Protein (Cat# AAA153127)
Full Name
Active Interleukin 1 Zeta (IL1z)
Gene Names
IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate)
Galectin 2 (GAL2), Active Protein (Cat# AAA153013)
Full Name
Active Galectin 2 (GAL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(PECAM-1 (platelet-endothelial cell adhesion molecule-1; also known as CD31) is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily. Expression is restricted to cells involved in circulation, especially endotheli)
Platelet/Endothelial Cell Adhesion Molecule (PECAM1), Active Protein (Cat# AAA153034)
Full Name
Active Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153040)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(PLA2G-VII which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s,)
Phospholipase A2 Group VII (LpPLA2), Active Protein (Cat# AAA153065)
Full Name
Active Phospholipase A2 Group VII (LpPLA2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. Human VGF precursor is 615 amino acids (aa) in length. It contains an 22 aa signal sequence plus a 593 aa mature re)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153074)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
TNF, Active Protein (Cat# AAA214338)
Full Name
Recombinant Rat TNF
Gene Names
Tnf; Tnfa; RATTNF; TNF-alpha
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL1B, Active Protein (Cat# AAA214339)
Full Name
Recombinant Rat IL1B
Gene Names
Il1b; IL-1F2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CXCL1, Active Protein (Cat# AAA214340)
Full Name
Recombinant Rat CXCL1
Gene Names
Cxcl1; Gro1; CINC-1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
GRO gamma (CXCL3), Active Protein (Cat# AAA214342)
Full Name
Recombinant Rat GRO gamma (CXCL3)
Gene Names
Cxcl3; Cinc2; Cinc-2; Gm1960
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL2, Active Protein (Cat# AAA214344)
Full Name
Recombinant Rat IL2
Purity
>95% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant BMP2, Human;Antibody: Rabbit Anti-Human BMP2 Ab)
Bone Morphogenetic Protein 2 (BMP2), Active Protein (Cat# AAA149222)
Full Name
Active Bone Morphogenetic Protein 2 (BMP2)
Gene Names
BMP2; BDA2; BMP2A; SSFSC
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
SDS-PAGE
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Active Protein (Cat# AAA149224)
Full Name
Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Gene Names
Cx3cl1; Cx3c; Scyd1
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
SDS-PAGE
Glial Cell Line Derived Neurotrophic Factor (GDNF), Active Protein (Cat# AAA149225)
Full Name
Active Glial Cell Line Derived Neurotrophic Factor (GDNF)
Gene Names
Gdnf; ATF; AI385739
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant PK2, Human;Antibody: Rabbit Anti-Human PK2 Ab)
Prokineticin 2 (PK2), Active Protein (Cat# AAA149227)
Full Name
Active Prokineticin 2 (PK2)
Gene Names
PROK2; BV8; HH4; PK2; KAL4; MIT1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)
Carcinoembryonic Antigen (CEA), Active Protein (Cat# AAA149229)
Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS-PAGE
High Mobility Group Protein 1 (HMGB1), Active Protein (Cat# AAA149232)
Full Name
Active High Mobility Group Protein 1 (HMGB1)
Gene Names
Hmgb1; p30; Hmg1; HMG-1; SBP-1
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
SDS-PAGE
Plasminogen Activator, Tissue (tPA), Active Protein (Cat# AAA149233)
Full Name
Active Plasminogen Activator, Tissue (tPA)
Gene Names
Plat; tPA; AU020998; AW212668; D8Ertd2e
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
SDS-PAGE
Pregnancy Associated Plasma Protein A (PAPPA), Active Protein (Cat# AAA149240)
Full Name
Active Pregnancy Associated Plasma Protein A (PAPPA)
Gene Names
PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Standard Curve (Sample)
C ReProtein (CRP), Active Protein (Cat# AAA149242)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
Purity: >98% as determined by SDS-PAGE.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Pricing
SDS-PAGE
Nitric Oxide Synthase 2, Inducible (NOS2), Active Protein (Cat# AAA149243)
Full Name
Active Nitric Oxide Synthase 2, Inducible (NOS2)
Gene Names
Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
SDS-PAGE
(Sample: Avtive recombinant CFL1, Human)
Cofilin 1, Non Muscle (CFL1), Active Protein (Cat# AAA149246)
Full Name
Active Cofilin 1, Non Muscle (CFL1)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant MSTN, Mouse;Antibody: Rabbit Anti-Mouse MSTN Ab)
Myostatin (MSTN), Active Protein (Cat# AAA149248)
Full Name
Active Myostatin (MSTN)
Gene Names
Mstn; Cmpt; Gdf8
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
SDS-PAGE
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA149251)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100a8; Mrp8
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(The standard curve of 4-Nitrophenol.Carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), initiated by addition 50uL of various concentrations of CES1 (dilute by assay buffer) to 50uL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400nm.One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 1396 pmol/min/ug.Specific Activity (pmol/min/ug) = ΔOD*F/T*NΔOD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T=TimeN=Amount of enzyme)
Carboxylesterase 1 (CES1), Active Protein (Cat# AAA149260)
Full Name
Active Carboxylesterase 1 (CES1)
Gene Names
CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
WB (Western Blot)
(Sample: Recombinant ITIH4, Human;Antibody: Rabbit Anti-Human ITIH4 Ab)
Inter Alpha-Globulin Inhibitor H4 (ITIH4), Active Protein (Cat# AAA149264)
Full Name
Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)
Gene Names
ITIH4; H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing
Bioactivity
(Figure. The binding activity of IFN2 with IFNa/bR2.)
Interferon Alpha 2, Active Protein (Cat# AAA150081)
Full Name
Active Interferon Alpha 2 (IFNa2)
Gene Names
IFNA2; IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure. The binding activity of TNFSF13B with ITGb1.Tumor necrosis factor ligand superfamily member 13B protein (TNFSF13B) also known as B-cell activating factor (BAFF) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Besides, Integrin Beta 1 (ITGb1) has been identified as an interactor of TNFSF13B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFSF13B and recombinant human ITGb1. Briefly, TNFSF13B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ITGb1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TNFSF13B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFSF13B and ITGb1 was shown in Figure 1, and this effect was in a dose dependent manner.)
B-Cell Activating Factor, Active Protein (Cat# AAA150113)
Full Name
Active B-Cell Activating Factor (BAFF)
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
(Figure. The binding activity of TAT with HSPA8)
Tyrosine Aminotransferase, Active Protein (Cat# AAA150132)
Full Name
Active Tyrosine Aminotransferase (TAT)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure. Cell proliferation of MCF-7 cells after stimulated with IGF1.)
Insulin Like Growth Factor 1, Active Protein (Cat# AAA150053)
Full Name
Active Insulin Like Growth Factor 1 (IGF1)
Gene Names
IGF1; IGF; MGF; IGFI; IGF-I
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
Glypican 3 (GPC3), Active Protein (Cat# AAA190412)
Full Name
Recombinant Human Glypican 3 (GPC3) Protein
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.