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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2500-2550 of 2875 product results
Bioactivity
(A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD() that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 ul assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.)
Lactate Dehydrogenase C (LDHC), Active Protein (Cat# AAA161921)
Full Name
Active Lactate Dehydrogenase C (LDHC)
Gene Names
Ldhc; Ldh3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant rat CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161777)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
IL2, Active Protein (Cat# AAA214344)
Full Name
Recombinant Rat IL2
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase IX (CA9) also known as membrane antigen MN and renal cell carcinoma (RCC) associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. rhCA9 correspond)
Carbonic Anhydrase IX (CA9), Active Protein (Cat# AAA153125)
Full Name
Active Carbonic Anhydrase IX (CA9)
Gene Names
CA9; MN; CAIX
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombinant human BMP-9.)
ALK-1, Active Protein (Cat# AAA258252)
Full Name
ALK-1 Protein, Canine, Recombinant (hFc Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Tumor protein p53 (TP53), also known as p53, cellular tumor antigen p53 has many mechanisms of anticancer function and plays a role in apoptosis, genomic stability, and inhibition of angiogenesis. TP53 act as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It also Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Besides, CREB Binding Protein (CREBBP) has been identified as an interactor of TP53, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TP53 and recombinant rat CREBBP. Briefly, TP53 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to CREBBP-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TP53 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TP53 and CREBBP was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of TP53 with CREBBP.)
Tumor Protein p53, Active Protein (Cat# AAA150105)
Full Name
Active Tumor Protein p53 (P53)
Gene Names
Tp53; p53; Trp53
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153043)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
FGF-2 (basic), Active Protein (Cat# AAA79132)
Full Name
Human FGF-2 (basic)
Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Human
Purity
> 98% by SDS-PAGE & silver stain
Pricing
SDS-PAGE
ICOS / AILIM / CD278, Active Protein (Cat# AAA173569)
Full Name
Recombinant Human ICOS / AILIM / CD278 Protein (His & Fc tag)
Gene Names
ICOS; AILIM; CD278; CVID1
Purity
>90% as determined by SDS-PAGE
Pricing
SDS-PAGE
IL-1 beta / IL1B, Active Protein (Cat# AAA173575)
Full Name
Recombinant Human IL-1 beta / IL1B Protein (pro form, His tag)
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Purity
>95 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Urokinase-type plasminogen activator receptor (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored protein. Besides regulating proteolysis, uPAR could also activate many intracellular signaling pathways that promote cell motility, invasion, proliferation and survival through cooperating with transmembrane receptors. uPAR is overexpressed across a variety of tumors and is associated with cancer invasion and metastasis. ITGb1 has been identified as an interactor of uPAR, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human uPAR and recombinant human ITGb1. Briefly, uPAR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-uPAR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human uPAR and recombinant human ITGb1 was shown in Figure 1, the EC50 for this effect is 0.165 ug/mL.)
Plasminogen Activator, Urokinase Receptor (uPAR), Active Protein (Cat# AAA161708)
Full Name
Active Plasminogen Activator, Urokinase Receptor (uPAR)
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse CD47-His at 10ug/mL (100uL/well) can bind mouse SIRPA-Fc, the EC50 of mouse SIRPA-Fc is 0.07-0.3 ug/mL.)
CD47, Active Protein (Cat# AAA258243)
Full Name
CD47 Protein, Mouse, Recombinant (His Tag)
Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL recombinant Activin A.)
ACVR2A, Active Protein (Cat# AAA257856)
Full Name
ACVR2A Protein, Human, Recombinant (hFc Tag)
Gene Names
ACVR2A; ACVR2; ACTRII
Purity
>97% as determined by SDS-PAGE
Pricing
Bioactivity
(FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A bindi)
Factor Related Apoptosis (FAS), Active Protein (Cat# AAA152951)
Full Name
Active Factor Related Apoptosis (FAS)
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)
Carcinoembryonic Antigen (CEA), Active Protein (Cat# AAA149229)
Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Western Blot Sample: Recombinant APOC3, Mouse; Antibody: Rabbit Anti-Mouse APOC3 Ab)
Apolipoprotein C3 (APOC3), Active Protein (Cat# AAA149252)
Full Name
Active Apolipoprotein C3 (APOC3)
Gene Names
Apoc3; apo-CIII; apoC-III
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 2), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)
Annexin V (ANXA5), Active Protein (Cat# AAA161721)
Full Name
Active Annexin V (ANXA5)
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
DMBT1 / Muclin, Active Protein (Cat# AAA173542)
Full Name
Recombinant Human DMBT1 / Muclin Protein (His tag)
Gene Names
DMBT1; SAG; GP340; SALSA; muclin
Purity
> 95 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
Complement Component 8b (C8b), Active Protein (Cat# AAA161841)
Full Name
Active Complement Component 8b (C8b)
Gene Names
C8B; C82
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07ug/mL in the presence of 10 ng/mL of mouse Wnt3a.)
DKK1, Active Protein (Cat# AAA257836)
Full Name
DKK1 Protein, Human, Recombinant (hFc Tag)
Gene Names
DKK1; SK; DKK-1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Osteopontin (OPN), Active Protein (Cat# AAA146622)
Full Name
Active Osteopontin (OPN)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(Complement Component 3 (C3) is a vital component of the complement system that plays a central role in immune response and inflammation. C3 is a b2 globulin synthesized by the liver, whose structure consists of two polypeptide chains, a and b, and is the most abundant complement component in serum. It has reported that P-Selectin (SELP) can interact with C3, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C3 and recombinant human SELP. Briefly, biotin-linked SELP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. When Recombinant mouse C3 is lmmobilized at 2 ug/mL (100 uL/well), the concentration of human SELP that produces 50% optimal binding response is found to be approximately 3.75 ug/mL.)
Complement Component 3 (C3), Active Protein (Cat# AAA161794)
Full Name
Active Complement Component 3 (C3)
Gene Names
C3; ASP; Plp; HSE-MSF; AI255234
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD58-His at 10 ug/mL (100 uL/well) can bind human CD2-Fc, the EC50 of human CD2-Fc is 100-350 ng/mL.)
CD2, Active Protein (Cat# AAA257956)
Full Name
CD2 Protein, Human, Recombinant (ECD, hFc Tag)
Gene Names
CD2; T11; SRBC; LFA-2
Purity
>90% as determined by SDS-PAGE
Pricing
Cancer Antigen 125, Active Protein (Cat# AAA11499)
Full Name
Cancer Antigen 125 (CA-125), Human
Gene Names
MUC16; CA125
Purity
> 95% (SDS-PAGE)
Pricing
Bioactivity
(Human IL-17E/IL-25 is coated at 2 ug/ml (100 ul/well) can bind Human IL-17 RB (CAT# ATGP4039) in a Functional ELISA assay.)
IL-17E/IL-25, Active Protein (Cat# AAA48395)
Full Name
Recombinant human IL-17E/IL-25 protein
Gene Names
IL25; IL17E
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
Application Data
MCP-2 (CCL8), Active Protein (Cat# AAA214330)
Full Name
Recombinant Mouse MCP-2 (CCL8)
Gene Names
Ccl8; HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
Purity
>95% as determined by SDS-PAGE
Pricing
CD106 (VCAM1), Active Protein (Cat# AAA14416)
Full Name
Human CD106 Recombinant (VCAM1)
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Serum Gonadotropin, Active Protein (Cat# AAA11497)
Full Name
Pregnant Mare Serum Gonadotropin (PMSG)
Pricing
Bioactivity
(Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of recombinant pig LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1 ug/mL LPS, and treated with certain concentrations (0.625 ug/mL-5 ug/mL) of rpLBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with recombinant pig LBP have shown in Figure 1.)
Lipopolysaccharide Binding Protein (LBP), Active Protein (Cat# AAA161848)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
LBP; LPSBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Sequence
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA150971)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
CXCL2, Active Protein (Cat# AAA214329)
Full Name
Recombinant Mouse CXCL2
Gene Names
Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-8 ug/mL.)
Epiregulin, Active Protein (Cat# AAA257906)
Full Name
Epiregulin Protein, Human, Recombinant (hFc Tag)
Gene Names
EREG; ER; Ep; EPR
Purity
>95% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Sample: Recombinant CIRBP, Mouse;Antibody: Rabbit Anti-Mouse CIRBP Ab)
Cold Inducible RNA Binding Protein (CIRBP), Active Protein (Cat# AAA21145)
Full Name
Active Cold Inducible RNA Binding Protein (CIRBP)
Reactivity
Mus musculus (Mouse)
Applications
Cell Culture, Activity Assay
Purity
>95%
Pricing
Application Data
Insulin (INS), Active Protein (Cat# AAA148171)
Full Name
Active Insulin (INS)
Reactivity
Bovine
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Procollagen I N-Terminal Propeptide is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces, which was are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, Langer-Saldino achondrogenesis, SED congenita, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. A functional binding ELISA assay was conducted to detect the interaction of recombinant rat PINP with recombinant human COL5a2. Briefly, biotin-linked PINP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to COL5a2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30 min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat PINP with recombinant human COL5a2 was shown in Figure 1, the EC50 for this effect is 9.18 ug/mL.)
Procollagen I N-Terminal Propeptide (PINP), Active Protein (Cat# AAA161809)
Full Name
Active Procollagen I N-Terminal Propeptide (PINP)
Gene Names
Col1a1; COLIA1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(TGF-? suppresses DC production but has no effect on DC activation.Bar graphs summarize the average production of IL-12, IL-6, and IL-10 from CD11cdnR (n?=?4) and wild-type (n?=?4) cultures.)
Interleukin-12 p40, Active Protein (Cat# AAA14439)
Full Name
Mouse Interleukin-12 p40 Recombinant
Gene Names
Il12b; p40; Il-12b; Il12p40; Il-12p40
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)
Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153090)
Full Name
Active Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 ug/ml (100 ul/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 ug/ml.)
Syndecan-4, Active Protein (Cat# AAA258200)
Full Name
Syndecan-4 Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Sdc4; Synd4; AA959608; AW108331; ryudocan; syndecan-4
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFalpha, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152994)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNF-ALPHA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(VEGF206-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC). HDLECs were stimulated with increasing amounts of human VEGF206.)
VEGF206, Active Protein (Cat# AAA14955)
Full Name
Recombinant Human Vascular Endothelial Growth Factor206
Gene Names
VEGFA; VPF; VEGF; MVCD1
Purity
> 75% by SDS-PAGE & Coomassie stain
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Cyclophilin A, Active Protein (Cat# AAA48477)
Full Name
Cyclophilin A, 1-165aa, Human, E Coli (Bioactivity Validated)
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Application Data
MCP-2 (CCL8), Active Protein (Cat# AAA214243)
Full Name
Recombinant Human MCP-2 (CCL8)
Gene Names
CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
FGF-2 (basic), Active Protein (Cat# AAA79225)
Full Name
Rat FGF-2 (basic)
Gene Names
Fgf2; bFGF; Fgf-2
Reactivity
Rat
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Bioactivity
(Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.Thi)
Lipopolysaccharide Binding Protein (LBP), Active Protein (Cat# AAA153087)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
Lbp; Ly88; Bpifd2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Apolipoprotein M (APOM) is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of lipocalin family proteins. It functions as a component of lipoprotein particles which play essential roles in fatty acid and cholesterol transport and metabolism. Alternative splicing generates a short isoform that lacks the N-terminal 72 amino acids. APOM is produced primarily by hepatocytes but also by renal tubule epithelial cells. The signal peptide is not cleaved and is required for APOM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney. The activity of recombinant mouse APOM was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in APOM. APOM was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 400, 200, 100, 30, 10, 3, 1 and 0.5 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rmAPOM and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rmAPOM and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >300 uM.)
Apolipoprotein M (APOM), Active Protein (Cat# AAA161884)
Full Name
Active Apolipoprotein M (APOM)
Gene Names
Apom; G3a; NG20; 1190010O19Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location. The activity of recombinant human CA5B was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA5B (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase VB, Mitochondrial (CA5B), Active Protein (Cat# AAA161903)
Full Name
Active Carbonic Anhydrase VB, Mitochondrial (CA5B)
Gene Names
CA5B; CA-VB
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 16 (IL-16), formerly known as lymphocyte chemoattractant factor or LCF, is a pro-inflammatory cytokine that is chemotactic for CD4 T lymphocytes, monocytes, and eosinophils. In addition to inducing chemotaxis, IL-16 can upregulate IL-2 receptor and HLA-DR4 expression, inhibit T cell receptor (TcR)/CD3-dependent activation, and promote repression of HIV-1 transcription.Besides,Interleukin 1 Alpha (IL1a) has been identified as an interactor of IL-16, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IL-16 and recombinant dog IL1a. Briefly, IL-16 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL1a-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL-16 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse IL-16 and recombinant dog IL1a was shown in Figure 1, the EC50 for this effect is 1.9 ug/mL.)
Interleukin 16 (IL16), Active Protein (Cat# AAA161675)
Full Name
Active Interleukin 16 (IL16)
Gene Names
Il16; mKIAA4048
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
COVID 19 Spike S1 Coronavirus, Active Protein (Cat# AAA60537)
Full Name
SARS-CoV-2 Spike S1 protein
Purity
>95%
Pricing
SDS-PAGE
BLMH, Active Protein (Cat# AAA48677)
Full Name
BLMH, 1-455aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
BLMH; BH; BMH
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Bioactivity
(Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein's specific biological action and is responsible for the interaction with its receptor. LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol. A functional binding ELISA assay was conducted to detect the interaction of recombinant human CGa/LHb and recombinant human Dopamine Receptor D1 (DRD1). Briefly, biotin-linked CGa/LHb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to DRD1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of CGa/LHb and DRD1 was shown in Figure 1, the EC50 for this effect is 0.644 ug/mL.)
Luteinizing Hormone (LH), Active Protein (Cat# AAA161734)
Full Name
Active Luteinizing Hormone (LH)
Gene Names
CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.