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ICC (Immunocytochemistry) (ICC staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FOXO1A on different lysates using anti-FOXO1A antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. High power)
Application Data (Rat splenocytes stained with Mouse anti Rat CD8 Beta:FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)
Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Medium power)
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)
WB (Western Blot) (Western Blot analysis of TUBB2A expression in transfected 293T cell line by TUBB2A monoclonal antibody. Lane 1: TUBB2A transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1.5ug/ml])
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in Jurkat.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in Raw 264.7.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (74.47kD).)
WB (Western Blot) (SF3B2 monoclonal antibody. Western Blot analysis of SF3B2 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SF3B2 on HeLa cell. [antibody concentration 60ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SF3B2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml].)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in NIH/3T3.)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (32.05kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in different cell lines.)
WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in Hela S3 NE.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1, 000 dilution.)
SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1(ABT-MLH1) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)
IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)
WB (Western Blot) (Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody. Lane 1: ARNT transfected lysate (86.6kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ARNT is ~0.03ng/ml as a capture antibod)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARNT monoclonal antibody Western Blot analysis of ARNT expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged SDCBP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SDCBP transfected lysate using SDCBP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SDCBP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SDCBP on HepG2 cell. [antibody concentration 35ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SDCBP on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SDCBP expression in transfected 293T cell line by SDCBP monoclonal antibody. Lane 1: SDCBP transfected lysate (32.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SDCBP monoclonal antibody, Western Blot analysis of SDCBP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between PTK2 and PLCG1 HeLa cells were stained with PTK2 rabbit purified polyclonal 1:1200 and PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PLCG1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLCG1 on HeLa cell. [antibody concentration 40ug/ml].)
WB (Western Blot) (PLCG1 monoclonal antibody. Western Blot analysis of PLCG1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1, 000 dilution.)
WB (Western Blot) (ATP2C1 monoclonal antibody Western Blot analysis of ATP2C1 expression in HeLa.)
WB (Western Blot) (Western blot analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATP2C1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ATP2C1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ATP2C1 transfected lysate using ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ATP2C1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody. Lane 1: ATP2C1 transfected lysate (100.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (42.61kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (GABPA monoclonal antibody (M07), clone 5C8 Western Blot analysis of GABPA expression in Hela S3 NE.)
WB (Western Blot) (CBX3/HP1 Gamma Antibody-Immunoprecipitation analysis of HeLa cell lysates using HP1-gamma mouse monoclonal antibody.)
WB (Western Blot) (CBX3/HP1 Gamma Antibody-Western blot detection of HP1-gamma in HeLa, 3T3, C6, COS7 and CHO-K1 cell lysates using HP1-gamma mouse monoclonal antibody (1:1000 dilution). Predicted band size: 22KDa. Observed band size:22KDa.)
ICC (Immunocytochemistry) (CBX3/HP1 Gamma Antibody-Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse monoclonal antibody (dilution 1:200).)
WB (Western Blot) (Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody. Lane 1: CRKL transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with PTK2 rabbit purified polyclonal 1:600 and CRKL mouse monoclonal antibody 1:100. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with GAB1 rabbit purified polyclonal 1:1200 and CRKL mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged CRKL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CRKL on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IP (Immunoprecipitation) (Immunoprecipitation of TRIM24 transfected lysate using TRIM24 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TRIM24 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM24 on HeLa cell. [antibody concentration 10ug/ml.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody Lane 1: TRIM24 transfected lysate (116.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TRIM24 monoclonal antibody Western Blot analysis of TRIM24 expression in Hela NE.)
WB (Western Blot) (TRIM24 monoclonal antibody Western Blot analysis of TRIM24 expression in IMR-32.)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in PC-12)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in Raw 264.7)
Application Data (Detection limit for recombinant GST tagged PGRMC2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGRMC2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml])
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in NIH/3T3)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)
WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)
WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)
Application Data (Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
WB (Western Blot) (ROCK2 monoclonal antibody (M02), clone 1E12 Western Blot analysis of ROCK2 expression in Hela S3 NE.)
ICC (Immunocytochemistry) (ICC staining DRD1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DRD1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DRD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DRD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DRD1 on mouse kidney cells lysates using anti-DRD1 antibody at 1/500 dilution.)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)
Application Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)
WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)
WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)
WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)
WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (HMGB2 monoclonal antibody (M04), clone 3D2 Western Blot analysis of HMGB2 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TPX2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 3164C6a-1])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TPX2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 3164C6a-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TPX2 mAb. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
WB (Western Blot) (Detection of human TPX2 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 3164C6a-1]Predicted molecular weight: 85 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-TPX2 monoclonal antibody.)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p120 catenin (ABT-p120) antibody. The HRP-conjugated)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Invasive ductal breast carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast Antibody was diluted at 1:200(4 degree overnight).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immmunohistochemistry) (Human ductal carcinoma of breast tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-p120 catenin (ABT-p120) Antibody)
IHC (Immunohistchemistry) (ENPEP/Aminopeptidase A Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry) (ENPEP/Aminopeptidase A Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot) (ENPEP/Aminopeptidase A Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENPEP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENPEP.)
ICC (Immunocytochemistry) (ENPEP/Aminopeptidase A Antibody-Anti-ENPEP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ENPEP.)
FCM (Flow Cytometry) (ENPEP/Aminopeptidase A Antibody-HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ENPEP antibody, and then analyzed by flow cytometry.)
FCM (Flow Cytometry) (ENPEP/Aminopeptidase A Antibody-Flow cytometry of HeLa cells, using anti-ENPEP antibody (Red), compared to a nonspecific negative control antibody (Blue).)
WB (Western Blot) (CSK monoclonal antibody. Western Blot analysis of CSK expression in Hela NE.)
WB (Western Blot) (Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody. Lane 1: CSK transfected lysate (50.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CSK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CSK transfected lysate using CSK monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CSK rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.74kD).)
WB (Western Blot) (PHGDH monoclonal antibody. Western Blot analysis of PHGDH expression in human liver.)
IP (Immunoprecipitation) (Immunoprecipitation of PHGDH transfected lysate using PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1-10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1-10ug/ml])
WB (Western Blot) (Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody. Lane 1: PHGDH transfected lysate (56.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FOXA1 monoclonal antibody (M02), clone 1B1 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining PKC alpha in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: 293)
ICC (Immunocytochemistry) (ICC staining CYP2E1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CYP2E1 in 293-T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CYP2E1 on human liver cells lysates using anti-CYP2E1 antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IP (Immunoprecipitation) (Immunoprecipitation of ALPPL2 transfected lysate using ALPPL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALPPL2 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody Lane 1: ALPPL2 transfected lysate (57.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ALPPL2 monoclonal antibody Western Blot analysis of ALPPL2 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)
WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A6 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to S100A6 on formalin-fixed paraffin-embedded human stomach carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of S100A6 expression in transfected 293T cell line by S100A6 monoclonal antibody. Lane 1: S100A6 transfected lysate (10.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (S100A6 monoclonal antibody. Western Blot analysis of S100A6 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
S100A6 (Protein S100-A6, S100 Calcium-binding Protein A6, Calcyclin, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) (Biotin)
Gene Names
S100A6; 2A9; PRA; 5B10; CABP; CACY
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot) (Anti-NP rabbit monoclonal antibody at 1:2000 dilution. Sample: Reocombinant Protein 10 ng Lane 1: H1N1 (A/Brevig Mission/1/1918) NP Lane 2: H1N1 (A/Brisbane/02/2018 NP Lane 3: H1N1 (A/California/07/2009) NP Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP Lane 5: H1N1 (A/Hawaii/70/2019) NP Lane 6: H1N1 (A/Michigan/45/2015 NP Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP Lane 9: H2N2 (A/Ann Arbor/6/1960) NP Lane 10: H3N2 (A/Aichi/2/1968) NP Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP Lane 12: H3N2 (A/Hong Kong/1/1968) NP Lane 13: H3N2 (A/Hong Kong/2671/2019) NP Lane 14: H3N2 (A/Hong Kong/45/2019) NP Lane 15: H3N2 (A/Hong Kong/4801/2014 NP Lane 16: H3N2 (A/Kansas/14/2017 NP Lane 17: H3N2 (A/Switzerland/9715293/2013) NP Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP Lane 19: H7N9 (A/Shanghai/2/2013) NP Lane 20: Influenza B (B/Brisbane/60/2008 NP Lane 21: Influenza B (B/Colorado/06/2017 NP Lane 22: Influenza B (B/Florida/4/2006) NP Lane 23: Influenza B (B/Phuket/3073/2013) NP Lane 24: Influenza B (B/Washington/02/2019) NP Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.)
Pan Influenza B Nucleoprotein Antibody, Rabbit MAb
Applications
Western Blot (WB), ELISA (EIA)
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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