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ICC (Immunocytochemistry) (ICC staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Syndecan 1 on NIH-3T3 cells lysates using anti-Syndecan 1 antibody at 1/500 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDB2 mAb. [Lot No. 2246C4a-1])
WB (Western Blot) (Detection of human DDB2 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2246C4a-1]Predicted molecular weight: 47 kDa)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GBA on diffewrent cells lysates using anti-GBA antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: 293T)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa NE.)
Application Data (Detection limit for recombinant GST tagged CHEK2 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PCNA transfected lysate using PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody Lane 1: PCNA transfected lysate (28.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCNA monoclonal antibody Western Blot analysis of PCNA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (54.45kD).)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged MCM2 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MCM2 transfected lysate using MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody. Lane 1: MCM2 transfected lysate (101.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MCM2 monoclonal antibody Western Blot analysis of MCM2 expression in HeLa NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (SWAP70 monoclonal antibody Western Blot analysis of SWAP70 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (34.25kD).)
Application Data (Detection limit for recombinant GST tagged SWAP70 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SWAP70 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SWAP70 expression in transfected 293T cell line by SWAP70 monoclonal antibody Lane 1: SWAP70 transfected lysate (69kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SWAP70 monoclonal antibody Western Blot analysis of SWAP70 expression in NIH/3T3)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPL19 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to RPL19 on formalin-fixed paraffin-embedded human small Intestine tissue. [antibody concentration 1~10ug/ml])
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in NIH/3T3.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in Jurkat.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in Raw 264.7.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in PC-12.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in HeLa.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in LNCaP.)
Application Data (Detection limit for recombinant GST tagged MAN1B1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAN1B1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in A-431.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody (AAA28517). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (AAA28517) at a dilution of 1:1000.)
ICC (Immunocytochemistry) (Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
ICC (Immunocytochemistry) (Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse herat tissue using anti-EDG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of EDG1 on SH-SY5Y cells lysates using anti-EDG1 antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat-liver tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-spleen tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.c-Myc Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). CYCS Monoclonal Antibody(4B10)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS Monoclonal Antibody.)
WB (Western Blot) (Western blot analysis of lysates from 1) Hela. 2) 293T. 3) 3T3. cells. ?Green? primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. ?Red? GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot) (Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) 293T. 3) 3T3. 4) Mouse Liver Tissue. 5) Rat Liver Tissue. 6) Rat Kidney Tissue using CYCS Monoclonal Antibody.)
WB (Western Blot) (ERG Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERG.)
WB (Western Blot) (CDK6 monoclonal antibody, Western Blot analysis of CDK6 expression in Jurkat.)
WB (Western Blot) (CDK6 monoclonal antibody. Western Blot analysis of CDK6 expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK2 and CDK6 HeLa cells were stained with CDK2 rabbit purified polyclonal 1:1200 and CDK6 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody. Lane 1: CDK6 transfected lysate (36.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.41kD).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Survivin Mouse mAb (AAA30823) diluted at 1:200 (4 degree overnight). 2. High-pressure and temperature Citric acid. pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot) (Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (AAA30823. 1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot) (Western blot analysis of 1) Hela. 2) 293. 3) PC12 using Survivin Monoclonal Antibody.)
Application Data (Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)
IP (Immunoprecipitation) (Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)
WB (Western Blot) (Western Blot detection against Immunogen (54.71kD).)
WB (Western Blot) (TARDBP monoclonal antibody Western Blot analysis of TARDBP expression in A-431.)
WB (Western Blot) (Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TARDBP is 0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of TARDBP transfected lysate using TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody. Lane 1: TARDBP transfected lysate (44.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS5 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody. Lane 1: RPS5 transfected lysate (22.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in NIH/3T3.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in Raw 264.7.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in PC-12.)
WB (Western Blot) (RPS5 monoclonal antibody, Western Blot analysis of RPS5 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Smad3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Smad3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad3 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Smad3 on A549 cell lysates using anti-Smad3 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining AQP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AQP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AQP1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-AQP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of AQP1 on different cells lysates using anti-AQP1 antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2:Jurkat Line 3: Human kidney)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX8 mAb. [Lot No. PAX8R1-3])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PAX8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. PAX8R1-3])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-PAX8 mAb. [Lot No. PAX8R1-3]Predicted molecular weight: 48 kDa)
WB (Western Blot) (Detection of PAX8 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. PAX8R1-3]Predicted molecular weight: 48 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-PAX8 monoclonal antibody.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (68.53kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in different cell lines.)
WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in Hela S3 NE.)
CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (Biotin)
IF (Immunofluorescence) (Immunofluorescence analysis of rat-spleen tissue. 1.Cyclophilin B Monoclonal Antibody(2B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of human-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.Calmodulin Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of 1) Jurkat. 2)293T. 3)Rat Liver Tissue. 4)3T3 with Cyclophilin B Mouse mAb diluted at 1:2.000.)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD43: FITC)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Medium power)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD43)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD43:Biotin)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD43:Azide Free)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Medium power)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)
WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody. Lane 1: CLK3 transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody. Lane 1: AKR1B10 transfected lysate (36kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (33.59kD).)
Application Data (Detection limit for recombinant GST tagged TCEA3 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (TCEA3 monoclonal antibody (M08), clone 4E11 Western Blot analysis of TCEA3 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCEA3 on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining CDK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CDK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-CDK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CDK1 on Jurkat cells lysates using anti-CDK1 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FGFR3 on Hela cells lysates using anti-FGFR3 antibody at 1/500 dilution.)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)
WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (AKT1 monoclonal antibody Western Blot analysis of AKT1 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody. Lane 1: AKT1 transfected lysate (55.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AKT1 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AKT1 transfected lysate using AKT1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AKT1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (78.54kD).)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)
Application Data (Detection limit for recombinant GST tagged CHUK is 0.03 ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (CHUK monoclonal antibody (M03), clone 4B8 Western Blot analysis of CHUK expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)
WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Prohibitin (PHB/3194). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IF (Immunofluorescence) (Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); Reddot is used to label the nuclei.)
SDS-PAGE (SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (PHB/3194). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).)
Application Data (Detection limit for recombinant GST tagged BUB1B is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody. Lane 1: BUB1B transfected lysate (119.5kD). Lane 2: Non-transfected lysate.)
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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