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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1800-1850 of 2583 product results
Bioactivity
(Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant rat TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rrTIMP1 (MW: 22.2 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rrTIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161745)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
Timp1; Timp; TIMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown in Figure 1, the EC50 for this effect is 2.82 ug/mL.)
Cluster Of Differentiation 28 (CD28), Active Protein (Cat# AAA161757)
Full Name
Active Cluster Of Differentiation 28 (CD28)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant rat GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant rat GSTa3 is >37000 pmol/min/ug.)
Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161768)
Full Name
Active Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta1; Yc1; Gsta3; Gsta5; GST AA; GST 2-2; GST Yc1; GST A3-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF23, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF23 and recombinant human FGFR1. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FGF23 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.17 ug/mL.)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA161772)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)
Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161662)
Full Name
Active Colony Stimulating Factor 3, Granulocyte (GCSF)
Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF is a monomeric glycoprotein that functions as a cytokine - it is a white blood cell growth factor. It also has some effects on mature cells of the immune system, such as inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GM-CSF and recombinant human CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human GM-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.04 ug/mL.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein (Cat# AAA161668)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Ciliary Neurotrophic Factor (CNTF) is a common extracellular polypeptide hormone who has neuroprotective effects on a variety of central and also peripheral nervous system neurons. It promotes differentiation and maturation of oligodendrocyte precursor ce)
Ciliary Neurotrophic Factor (CNTF), Active Protein (Cat# AAA152949)
Full Name
Active Ciliary Neurotrophic Factor (CNTF)
Gene Names
CNTF; HCNTF
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The chemotactic effect of NAP3 on THP-1 cells)
Chemokine (C-X-C Motif) Ligand 1 (CXCL1), Active Protein (Cat# AAA152959)
Full Name
Active Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(human IL12A is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded b)
Interleukin 12A (IL12A), Active Protein (Cat# AAA152962)
Full Name
Active Interleukin 12A (IL12A)
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.)
Interleukin 2 (IL2), Active Protein (Cat# AAA152964)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.)
Interleukin 2 (IL2), Active Protein (Cat# AAA152965)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; POIL2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells)
Interleukin 2 (IL2), Active Protein (Cat# AAA152966)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; POIL2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(IL-2(Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to r)
Interleukin 2 (IL2), Active Protein (Cat# AAA152967)
Full Name
Active Interleukin 2 (IL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target c)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA152974)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant human MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rhMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 °C for 2 hours. The activated rhMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rhMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human MMP1 is > 140 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA152977)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Gene Names
MMP1; CLG; CLGN
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family .It is a gelatinase A, 72 kDa type IV collagenase which can hydrolyze gelatin under certain conditions.)
Matrix Metalloproteinase 2 (MMP2), Active Protein (Cat# AAA152978)
Full Name
Active Matrix Metalloproteinase 2 (MMP2)
Gene Names
MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upo)
Matrix Metalloproteinase 7 (MMP7), Active Protein (Cat# AAA152979)
Full Name
Active Matrix Metalloproteinase 7 (MMP7)
Gene Names
MMP7; MMP-7; MPSL1; PUMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152986)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFalpha, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152992)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152993)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNF-beta.)
Tumor Necrosis Factor Beta (TNFb), Active Protein (Cat# AAA152995)
Full Name
Active Tumor Necrosis Factor Beta (TNFb)
Gene Names
LTA; TNFB
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasmi)
Plasminogen Activator, Urokinase Receptor (uPAR), Active Protein (Cat# AAA152998)
Full Name
Active Plasminogen Activator, Urokinase Receptor (uPAR)
Gene Names
Plaur; Cd87; uPAR; u-PAR
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Interferon alpha-2, Active Protein (Cat# AAA114580)
Full Name
Recombinant Human Interferon alpha-2
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Tumor-associated Calcium Signal Transducer 2/TROP-2, Active Protein (Cat# AAA177964)
Full Name
Recombinant Human Tumor-associated Calcium Signal Transducer 2/TROP-2 (248AA, C-Fc)
Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Purity
>95% as determined by reducing SDS-PAGE
Pricing
SDS-PAGE
Carbonic Anhydrase IX / CA9, Active Protein (Cat# AAA173576)
Full Name
Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His tag)
Gene Names
CA9; MN; CAIX
Purity
> 95 % as determined by SDS-PAGE
Pricing
SDS-PAGE
IL1R1 / CD121a, Active Protein (Cat# AAA173585)
Full Name
Recombinant Mouse IL1R1 / CD121a Protein (His tag)
Gene Names
Il1r1; IL-iR; CD121a; CD121b; IL-1R1; Il1r-1
Purity
> 95 % as determined by SDS-PAGE
Pricing
Bioactivity
B-Cell Receptor CD22 (CD22), Active Protein (Cat# AAA243725)
Full Name
Recombinant Human B-Cell Receptor CD22 (CD22), Partial (Active)
Gene Names
CD22; SIGLEC2; SIGLEC-2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Tumor Necrosis Factor Receptor Superfamily Member 17 (TNFRSF17), Active Protein (Cat# AAA243734)
Full Name
Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 17 (TNFRSF17), Partial (Active)
Gene Names
TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
(Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 2.5-10 ug/mL.)
BMPR1A/ALK-3, Active Protein (Cat# AAA257883)
Full Name
BMPR1A/ALK-3 Protein, Human, Recombinant (His Tag)
Gene Names
BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
Purity
>85% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit FGF-acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.5-2.5 ng/ml.)
FGFR2, Active Protein (Cat# AAA257933)
Full Name
FGFR2 Protein, Human, Recombinant (His & hFc Tag)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Purity
>90% as determined by SDS-PAGE
Pricing
SDS-PAGE
Hemopexin, Active Protein (Cat# AAA257938)
Full Name
Hemopexin Protein, Human, Recombinant (His Tag)
Gene Names
HPX; HX
Purity
>96% as determined by SDS-PAGE
Pricing
Application Data
Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Active Protein (Cat# AAA146598)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
Interferon Gamma (IFNg), Active Protein (Cat# AAA146601)
Full Name
Active Interferon Gamma (IFNg)
Gene Names
IFNG; IFG; IFI
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
Insulin Like Growth Factor 1 (IGF1), Active Protein (Cat# AAA146604)
Full Name
Active Insulin Like Growth Factor 1 (IGF1)
Gene Names
IGF1; MGF; IGFI; IGF-I
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
Interleukin 6 (IL6), Active Protein (Cat# AAA146610)
Full Name
Active Interleukin 6 (IL6)
Gene Names
Il6; Il-6
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA146615)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
Tumor Necrosis Factor Beta (TNFb), Active Protein (Cat# AAA146616)
Full Name
Active Tumor Necrosis Factor Beta (TNFb)
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Osteopontin (OPN), Active Protein (Cat# AAA146622)
Full Name
Active Osteopontin (OPN)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant HRG, Human;Antibody: Rabbit Anti-Human HRG Ab)
Histidine Rich Glycoprotein (HRG), Active Protein (Cat# AAA146623)
Full Name
Active Histidine Rich Glycoprotein (HRG)
Gene Names
HRG; HPRG; HRGP; THPH11
Reactivity
Homo sapiens (Human)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Pricing
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant LMTK3, Human;Antibody: Rabbit Anti-Human LMTK3 Ab)
Lemur Tyrosine Kinase 3 (LMTK3), Active Protein (Cat# AAA146624)
Full Name
Active Lemur Tyrosine Kinase 3 (LMTK3)
Gene Names
LMTK3; LMR3; TYKLM3; PPP1R101
Reactivity
Homo sapiens (Human)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>95%
Pricing
Application Data
Interleukin 15 (IL15), Active Protein (Cat# AAA148129)
Full Name
Active Interleukin 15 (IL15)
Gene Names
IL15; IL-15
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Interleukin 16 (IL16), Active Protein (Cat# AAA148141)
Full Name
Active Interleukin 16 (IL16)
Gene Names
IL16; LCF; NIL16; PRIL16; prIL-16
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Vascular Endothelial Growth Factor 121 (VEGF121), Active Protein (Cat# AAA148143)
Full Name
Active Vascular Endothelial Growth Factor 121 (VEGF121)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
WB (Western Blot)
(Sample: Recombinant MMP9, Porcine; Antibody: Rabbit Anti-Porcine MMP9 Ab)
Matrix Metalloproteinase 9 (MMP9), Active Protein (Cat# AAA149237)
Full Name
Active Matrix Metalloproteinase 9 (MMP9)
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Sus scrofa; Porcine (Pig)
Applications
Activity Assay, Cell Culture
Purity
>94%
Pricing
SDS-PAGE
Interleukin 2 (IL2), Active Protein (Cat# AAA148221)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
(Figure. Cell proliferation of Jurkat cells after stimulated with IL1a.Interleukin 1 alpha (IL1) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL1 is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. To test the effect of IL1a on cell proliferation, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well with 2% serum standard 1640 including various concentrations of recombinant human IL1a. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Proliferation of Jurkat cells after incubation with IL1a for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL1a for 96h. The result was shown in Figure 2. It was obvious that IL1a significantly increased cell viability of Jurkat cells.(A) Jurkat cells cultured in 1640, stimulated with 10ng/mL IL1a for 96h; (B) Unstimulated Jurkat cells cultured in1640 for 96h.)
Interleukin 1 Alpha, Active Protein (Cat# AAA150058)
Full Name
Active Interleukin 1 Alpha (IL1a)
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS_PAGE
(Sample: Active recombinant SOD3, Human)
Superoxide Dismutase 3, Extracellular, Active Protein (Cat# AAA150072)
Full Name
Active Superoxide Dismutase 3, Extracellular (SOD3)
Gene Names
SOD3; EC-SOD
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
(IL-1 (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It has been elucidated that IL-1 stimulation of cells activates MMP-9 (matrix metalloproteinases-9) secretion by the activation of the dual signalling pathways. Thus, a stimulation assay of IL-1 was conducted using 3T3 cell line, and the MMP-9 activity was detected through gel zymography. Briefly, 1×106 3T3 cells were cultured overnight in 5%DMEM, washed with serum-free medium and then stimulated with different concentrations of IL-1 for 20h, and cell lysates were collected to measure MMP-9 activity. Cell lysates samples were denatured by SDS loading buffer, electrophoresed through sodium dodecyl sulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and coomassiebrilliant blue (CBB)-stained, MMPs hydrolyzed gelatin nearby, indicated by the white binds on the gel. Result Increased MMP-9 activity in 3T3 cells due to the stimulation of IL-1 was shown in Figure 1.Figure. Activation of MMP-9 by IL-1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150093)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
(Figure. Cell proliferation of spleen cells after stimulated with GP39.)
Chitinase-3-like Protein 1, Active Protein (Cat# AAA150109)
Full Name
Active Chitinase-3-like Protein 1 (CHI3L1)
Gene Names
Chi3l1; Chil1; GP-39; CGP-39
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Wingless-type MMTV integration site family, member 16 is a protein that in humans is encoded by the WNT16 gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5 termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. Besides, Tubulin Beta 3 (TUBb3) has been identified as an interactor of WNT16, thus a binding ELISA assay was conducted to detect the interaction of recombinant human WNT16 and recombinant human TUBb3. Briefly, WNT16 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to TUBb3-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-WNT16 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of WNT16 and TUBb3 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of WNT16 with TUBb3.)
Wingless Type MMTV Integration Site Family, Member 16, Active Protein (Cat# AAA150149)
Full Name
Active Wingless Type MMTV Integration Site Family, Member 16 (WNT16)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.