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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1850-1900 of 2875 product results
Bioactivity
(GSTa2 (Glutathione S-transferase a2) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant mouse GSTa2, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant mouse GSTa2 is >50000 pmol/min/ug.)
Glutathione S Transferase Alpha 2 (GSTa2), Active Protein (Cat# AAA161869)
Full Name
Active Glutathione S Transferase Alpha 2 (GSTa2)
Gene Names
Gsta2; Gstc2; Gst2-2; Gstc-2
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a member of the immunoglobulin subfamily. CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. CD80 and CD86 are expressed on antigen presenting cells and CD86 is primary ligand for CD28. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD28 and recombinant human CD86. Briefly, CD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD86-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD28 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.07 ug/mL.)
Cluster Of Differentiation 28 (CD28), Active Protein (Cat# AAA161758)
Full Name
Active Cluster Of Differentiation 28 (CD28)
Gene Names
Cd28; CD28RNA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.6-3 ug/mL.)
FGF17, Active Protein (Cat# AAA258010)
Full Name
FGF17 Protein, Human, Recombinant
Gene Names
FGF17; HH20; FGF-13; FGF-17
Purity
>95% as determined by SDS-PAGE
Pricing
ELISA
(Biotinylated TGFB-1 ELISAImmobilized Biotinylated Human TGF Beta1 at 1ug/ml (100ul/Well). Response curve for TGFR2 with alinear range of 2-100 ng/ml was determined by ELISA)
TGF Beta-1, Active Protein (Cat# AAA76386)
Full Name
Biotinylated Human TGF Beta-1 Recombinant
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Purity
>90%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Figure. The binding activity of PSA with PDIA3.Puromycin Sensitive Aminopeptidase (PSA) also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) is used as a biomarker to detect damage to the kidneys, and that may be used to help idenfity certain kidney disorders. It is found at high levels in the urine when there are kidney problems. PSA has been proposed to function in a variety of processes, including metabolism of neuropeptidase, regulation of the cell cycle, and hydrolysis of proteasomal products to amino acids. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of PSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PSA and recombinant human PDIA3. Briefly, PSA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PSA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PSA and PDIA3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Puromycin Sensitive Aminopeptidase, Active Protein (Cat# AAA150133)
Full Name
Active Puromycin Sensitive Aminopeptidase (PSA)
Gene Names
NPEPPS; PSA; AAP-S; MP100
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
Interleukin 4 (IL4), Active Protein (Cat# AAA146608)
Full Name
Active Interleukin 4 (IL4)
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
SDS-PAGE
(Sample: Avtive recombinant CFL1, Human)
Cofilin 1, Non Muscle (CFL1), Active Protein (Cat# AAA149246)
Full Name
Active Cofilin 1, Non Muscle (CFL1)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
SDS-PAGE
S100 Calcium Binding Protein A8 (S100A8), Active Protein (Cat# AAA149251)
Full Name
Active S100 Calcium Binding Protein A8 (S100A8)
Gene Names
S100a8; Mrp8
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(One unit of enzyme activity is defined as the 1 ug of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37 degree C. The specific activity of recombinant human CA4 is > 15 pmol/min/ug.)
Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161900)
Full Name
Active Carbonic Anhydrase IV (CA4)
Gene Names
CA4; CAIV; Car4; RP17
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Urokinase Plasminogen Activator (uPA), also known as u-plasminogen activator or urokinase, is a highly-specific serine protease from the peptidase S1 family that cleaves plasminogen to form plasmin making it a key player in the plasminogen activator (PA) system. Expression of uPA is minimal in normal cells but is increased several fold in tumor cells by extracellular stimuli elevated in cancer and corresponds to poor outcomes in several types of cancer. Therefore, uPA has been identified as an excellent target for therapeutic development through inhibition of protease activity or though inhibition of uPA-dependent signaling while in complex with uPA receptor (uPAR). The activity assay of uPA was measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The reaction was performed in 50 mM Tris, 0.01% Tween-20, pH 8.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 200 uM Substrate. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse uPA is >2000 pmol/min/ug.)
Plasminogen Activator, Urokinase (uPA), Active Protein (Cat# AAA161706)
Full Name
Active Plasminogen Activator, Urokinase (uPA)
Gene Names
Plau; uPA; u-PA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
ACE2, Active Protein (Cat# AAA119897)
Full Name
Recombinant Human ACE2 protein
Applications
Immunogen
Purity
>90% as determined by SDS-PAGE
Bioactivity
(HTRF assay for PRKCH; activity 1 uM STK S1 substrate was incubated with different concentrations of PRKCH; protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.)
Application Data
CD32B/Fcgr2b, Active Protein (Cat# AAA257858)
Full Name
CD32B/Fcgr2b Protein, Human, Recombinant (ECD, His Tag)
Gene Names
FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Rat ICOS ligand hFc at 2 ug/mL (100 uL/well) can bind Mouse ICOS His, the EC50 of Mouse ICOS His is 150-800 ng/mL.)
ICOS, Active Protein (Cat# AAA258165)
Full Name
ICOS Protein, Mouse, Recombinant (His Tag)
Gene Names
Icos; H4; CCLP; AILIM; CRP-1; Ly115
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD70 at 10 ug/mL (100 ul/well) can bind human CD27 with a linear range of 40-60 ng/ml.)
CD70, Active Protein (Cat# AAA257927)
Full Name
CD70 Protein, Human, Recombinant (hFc Tag)
Gene Names
CD70; CD27L; CD27LG; TNFSF7
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse CD27-His at 10 ug/mL (100 uL/well) can bind mouse CD70, the EC50of mouse CD70 is 10-50 ng/mL.)
CD70, Active Protein (Cat# AAA258236)
Full Name
CD70 Protein, Mouse, Recombinant (mFc Tag)
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7
Purity
>95% as determined by SDS-PAGE
Pricing
Adiponectin, Active Protein (Cat# AAA78131)
Full Name
Adiponectin, human, native
Gene Names
ADIPOQ; ADN; APM1; ACRP30
Purity
Affinity and hydrophobic interaction chromatography
Pricing
Bioactivity
FGL1, Active Protein (Cat# AAA177972)
Full Name
Recombinant Cynomolgus FGL1 (C-6His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant EMILIN1, Human)
Elastin Microfibril Interface Located Protein 1 (EMILIN1), Active Protein (Cat# AAA161733)
Full Name
Active Elastin Microfibril Interface Located Protein 1 (EMILIN1)
Gene Names
EMILIN1; EMI; gp115; EMILIN
Reactivity
Homo sapiens (Human)
Applications
Activity Assay
Purity
>80%
Pricing
Bioactivity
(Figure 2. The chemotactic effect of NAP3 on THP-1 cells)
Chemokine (C-X-C Motif) Ligand 1 (CXCL1), Active Protein (Cat# AAA152959)
Full Name
Active Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
Vascular Endothelial Growth Factor C (VEGFC), Active Protein (Cat# AAA148165)
Full Name
Active Vascular Endothelial Growth Factor C (VEGFC)
Gene Names
VEGFC; VRP; Flt4-L; LMPH1D
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant DNASE1, Human;Antibody: Rabbit Anti-Human DNASE1 Ab)
Deoxyribonuclease I (DNASE1), Active Protein (Cat# AAA148188)
Full Name
Active Deoxyribonuclease I (DNASE1)
Gene Names
DNASE1; DNL1; DRNI
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 ug/ml (100 ul/well) can bind human EphB4 with a linear ranger of 1.56-12.5 ng/ml.)
Ephrin B2, Active Protein (Cat# AAA257942)
Full Name
Ephrin B2 Protein, Human, Recombinant (His Tag)
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
Beta-Defensin 1 (DEFB1), Active Protein (Cat# AAA214324)
Full Name
Recombinant Mouse Beta-Defensin 1 (DEFB1)
Gene Names
Defb1; BD-1; AW260221
Reactivity
Mouse (M. musculus)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
M-CSF (CSF1), Active Protein (Cat# AAA214245)
Full Name
Recombinant Human M-CSF (CSF1)
Gene Names
CSF1; MCSF; CSF-1
Purity
>95% as determined by SDS-PAGE
Pricing
Purity Info
PDE9A, Active Protein (Cat# AAA73107)
Full Name
PDE9A, Active
Gene Names
PDE9A; HSPDE9A2
Purity
>90%
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A1+B1+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A1+B1+G1), Active Protein (Cat# AAA60345)
Full Name
Recombinant AMPK Complex (A1+B1+G1) protein
Purity
>=90%
Pricing
Application Data
(IL-1 (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It has been elucidated that IL-1 stimulation of cells activates MMP-9 (matrix metalloproteinases-9) secretion by the activation of the dual signalling pathways. Thus, a stimulation assay of IL-1 was conducted using 3T3 cell line, and the MMP-9 activity was detected through gel zymography. Briefly, 1×106 3T3 cells were cultured overnight in 5%DMEM, washed with serum-free medium and then stimulated with different concentrations of IL-1 for 20h, and cell lysates were collected to measure MMP-9 activity. Cell lysates samples were denatured by SDS loading buffer, electrophoresed through sodium dodecyl sulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and coomassiebrilliant blue (CBB)-stained, MMPs hydrolyzed gelatin nearby, indicated by the white binds on the gel. Result Increased MMP-9 activity in 3T3 cells due to the stimulation of IL-1 was shown in Figure 1.Figure. Activation of MMP-9 by IL-1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150093)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
Golgi Protein 73 (GP73), Active Protein (Cat# AAA148195)
Full Name
Active Golgi Protein 73 (GP73)
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Coagulation Factor VII (F7), Active Protein (Cat# AAA148198)
Full Name
Active Coagulation Factor VII (F7)
Reactivity
Rat
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant SEMA3A, Human;Antibody: Rabbit Anti-Human SEMA3A Ab)
Semaphorin 3A (SEMA3A), Active Protein (Cat# AAA148218)
Full Name
Active Semaphorin 3A (SEMA3A)
Gene Names
SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Bioactivity
(High Mobility Group Protein 1 (HMG1), also known as high mobility group box 1 proteinbelongs to high mobility group and contains HMG-box domain. HMG1 is one of the most important chromatin proteins. This nuclear protein organizes the DNA and regulates transcription. It supports transcription of many genes in interactions with many transcription factors. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human TP53. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TP53-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of HMG1 and TP53 was shown in Figure 1, and this effect was in a dose dependent manner.)
High Mobility Group Protein 1 (HMG1), Active Protein (Cat# AAA149231)
Full Name
Active High Mobility Group Protein 1 (HMG1)
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Application Data
GM-CSF (CSF2), Active Protein (Cat# AAA214310)
Full Name
Recombinant Mouse GM-CSF (CSF2)
Gene Names
Csf2; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
KGF (FGF7), Active Protein (Cat# AAA214319)
Full Name
Recombinant Mouse KGF (FGF7)
Gene Names
Fgf7; Kgf
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
LIX (CXCL5), Active Protein (Cat# AAA214332)
Full Name
Recombinant Mouse LIX (CXCL5)
Gene Names
Cxcl5; LIX; Cxcl6; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized mouse VEGF164 at 10 ug/ml (100 ul/well) can bind mouse KDR-Fc, The EC50 of mouse KDR-Fc is 0.11-0.27 ug/ml.)
VEGFR2/KDR, Active Protein (Cat# AAA258218)
Full Name
VEGFR2/KDR Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-1 ug/mL in the presence of 10 ng/mL of recombinant mouse NGF.)
TrkA, Active Protein (Cat# AAA258230)
Full Name
TrkA Protein, Mouse, Recombinant (His Tag)
Gene Names
Ntrk1; Tkr; trk; TrkA; C80751
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.1-1 ug/mL.)
Neuregulin-1, Active Protein (Cat# AAA258257)
Full Name
Neuregulin-1 Protein, Canine, Recombinant (EGF Domain, mFc Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-2 ug/mL.)
PDGF-C, Active Protein (Cat# AAA258164)
Full Name
PDGF-C Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Pdgfc; PDGF-C; AI647969; 1110064L01Rik
Purity
>80% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human PD1-His at 10 ug/mL (100 ul/well) can bind mouse PD-L2-Fc, The EC50 of mouse PD-L2-Fc is 0.95 ug/mL.)
PD-L2, Active Protein (Cat# AAA258204)
Full Name
PD-L2 Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Pdcd1lg2; Btdc; B7-DC; PD-L2; F730015O22Rik
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 1.5-7.5 ug/mL.)
BMPRIB, Active Protein (Cat# AAA258113)
Full Name
BMPRIB Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Bmpr1b; Alk6; SKR6; ALK-6; Acvrlk6; BMPR-1B; BMPR-IB; CFK-43a; AI385617; AV355320
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human uPAR at 5 ug/ml (100 ul/well) can bind biotinylated human UPA with a linear ranger of 40-1000 ng/ml.)
uPAR/PLAUR, Active Protein (Cat# AAA257953)
Full Name
uPAR/PLAUR Protein, Human, Recombinant (His Tag)
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Purity
>98% as determined by SDS-PAGE
Pricing
SDS-PAGE
SHP-1, Active Protein (Cat# AAA48446)
Full Name
SHP-1,243-541aa, Human, E Coli (Bioactivity Validated)
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Bioactivity
(Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TIMP4 and recombinant human MMP2. Briefly, TIMP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TIMP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TIMP4 and MMP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Tissue Inhibitors Of Metalloproteinase 4, Active Protein (Cat# AAA150077)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)
Gene Names
TIMP4; TIMP-4
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Bioactivity
(Figure. The binding activity of IFN2 with IFNa/bR2.)
Interferon Alpha 2, Active Protein (Cat# AAA150081)
Full Name
Active Interferon Alpha 2 (IFNa2)
Gene Names
IFNA2; IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Laminin Gamma 1 (LAMC1), also known as LAMB2, is a member of the laminin family of proteins. LAMC1 is expressed in a variety of tissues and organs, including liver, kidney, heart, and brain. Its expression level is usually regulated by a variety of factors, such as hormones, growth factors, and cytokines. At the cellular level, LAMC1 is mainly distributed in the basement membrane and extracellular matrix of cells, and plays the role of cell adhesion, migration, differentiation and signal transduction by binding to cell surface receptors. Besides, Laminin Alpha 2 (LAMa2) has been identified as an interactor of LAMC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human LAMC1 and recombinant mouse LAMa2. Briefly, LAMC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LAMa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-LAMC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human LAMC1 and recombinant mouse LAMa2 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Laminin Gamma 1 (LAMC1), Active Protein (Cat# AAA161877)
Full Name
Active Laminin Gamma 1 (LAMC1)
Gene Names
LAMC1; LAMB2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Fc receptor with low affinity for IgG (Fc gamma RIII, CD16) is encoded by two nearly identical genes, Fc gamma RIII-A and Fc gamma RIII-B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FcgR3A is expressed in almost all white blood cells and is primarily responsible for the ADCC action of NK cells and the clearance of antibody-antigen immune complexes by macrophages. FcgR3B is mainly expressed in neutrophils and can stimulate the release of superoxide in neutrophils. A functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FcgR3A and mouse IgG. Briefly, biotin-linked FcgR3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IgG-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FcgR3A and mouse IgG was shown in Figure 1, the EC50 for this effect is 0.26 ug/mL.)
Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A), Active Protein (Cat# AAA161837)
Full Name
Active Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A)
Gene Names
Fcgr4; Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
GH1, Active Protein (Cat# AAA214221)
Full Name
Recombinant Human GH1
Gene Names
GH1; GH; GHN; GH-N; GHB5; IGHD2; hGH-N; IGHD1A; IGHD1B
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
HCC-2 (CCL15), Active Protein (Cat# AAA214226)
Full Name
Recombinant Human HCC-2 (CCL15)
Gene Names
CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 ug/ml (100 ul/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 ug/ml.)
Midkine, Active Protein (Cat# AAA257854)
Full Name
Midkine Protein, Human, Recombinant
Gene Names
MDK; MK; ARAP; NEGF2
Purity
>95% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.