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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1700-1750 of 2875 product results
Bioactivity
Siglec-10, Active Protein (Cat# AAA177946)
Full Name
Recombinant Human Siglec-10 (C-mFc)
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Purity
>90% as determined by reducing SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs. The activity of recombinant human CA13 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA13 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase XIII (CA13), Active Protein (Cat# AAA161906)
Full Name
Active Carbonic Anhydrase XIII (CA13)
Gene Names
CA13; CAXIII
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant Human HVEM-His at 10 ug/ml (100 ul/well) can bind human BTLA-Fc with a linear range of 1.28-20 ug/ml.)
HVEM, Active Protein (Cat# AAA257872)
Full Name
HVEM Protein, Human, Recombinant (ECD, His Tag)
Gene Names
TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 ug/ml (100 ul/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/ml.)
CD200R, Active Protein (Cat# AAA258144)
Full Name
CD200R Protein, Mouse, Recombinant (His & hFc Tag)
Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 ug/ml in the presence of 50 ng/mL of recombinant human BMP4.)
NBL1, Active Protein (Cat# AAA257835)
Full Name
NBL1 Protein, Human, Recombinant (His Tag)
Gene Names
NBL1; NB; DAN; NO3; DAND1; D1S1733E
Purity
>98% as determined by SDS-PAGE
Pricing
Application Data
(The purity of PDE1C was determined to be >90% by densitometry. Approx MW 99kDa)
PDE1C, Active Protein (Cat# AAA73095)
Full Name
PDE1C, Active
Gene Names
PDE1C; Hcam3; hCam-3; cam-PDE 1C
Purity
>90%
Pricing
Application Data
Tumor Necrosis Factor Beta (TNFb), Active Protein (Cat# AAA146616)
Full Name
Active Tumor Necrosis Factor Beta (TNFb)
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
SDS-PAGE
Interleukin 17D (IL17D), Active Protein (Cat# AAA148227)
Full Name
Active Interleukin 17D (IL17D)
Gene Names
IL17D; IL-17D
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Application Data
(Generation of conditional TAK1-deficient mice.Colony formation by BM cells from Map3k7)
Granulocyte Colony Stimulating Factor, Active Protein (Cat# AAA76390)
Full Name
Mouse Granulocyte Colony Stimulating Factor Recombinant
Gene Names
Csf3; Csfg; G-CSF; MGI-IG
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Human RAET1L/ULBP6 is coated at 10 ug/ml (100 ul/well) can bind Human NKG2D (CAT# ATGP3662) in a Functional ELISA assay.)
ULBP-6/RAET1L, Active Protein (Cat# AAA48398)
Full Name
Recombinant human ULBP-6/RAET1L Protein
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
DuSP3, Active Protein (Cat# AAA48723)
Full Name
DuSP3, 1-185aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
DUSP3; VHR
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Bioactivity
(VCP is an ATPase Associated with diverse cellular Activities (AAA) that has a key role in maintaining cellular homeostasis. VCP is ubiquitously expressed in tissues. At cellular level, VCP functions in many cellular compartments; VCP localizes mainly in the cytoplasm, while a smaller fraction binds to organelles or localizes in the nucleus, where it is implicated in different pathways. VCP assembles and acts as a homo-hexamer, in which each monomer is structured in an N-terminal domain that interacts with adaptors and co-factors; in two ATPase domains, D1 and D2, that by hydrolyzing ATP concur, respectively, in the hexamer formation and in supporting VCP activity; and in a C-terminal domain that binds to a small subset of co-factors and adaptors cooperating with D2 activity. The main role of VCP is to recognize and to extract ubiquitinated proteins from membranes, protein complexes, protein aggregates, or chromatin, modulating their ubiquitination or de-ubiquitination, and to enhance their degradation through the UPS or the autophagic pathway. The activity of recombinant human VCP was measured by its ability to hydrolyze the substrate ATP to phosphate which was detected by the malachite green phosphate detection Kit (Beyotime # S0196M). The reaction was performed in the assay buffer 20 mM HEPES, 13 mM NaCl and 1% glycerol, pH 8.5, initiated by addition 27 uL of various concentrations of VCP (diluted by assay buffer) to 3 uL of 100 mM ATP and 100 mM MgCl2 mixture. The final well serves as a negative control with no VCP, replaced with 27 uL assay buffer. Incubated at 37 degree C for 5min, then stop the reaction by quickly freezing samples in the dry ice and ethanol. 4 ul samples was added to 196 ul assay buffer after thawing at room temperature followed by adding 70 ul phosphate detection agent and incubated at room temperature for 30min, then read at a wavelength of 630 nm. The specific activity of recombinant human VCP is >1000 pmol/min/ug.)
Valosin Containing Protein (VCP), Active Protein (Cat# AAA161891)
Full Name
Active Valosin Containing Protein (VCP)
Gene Names
VCP; p97; TERA; ALS14; IBMPFD
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Mouse EPHA5 at 2 ug/ml (100 ul/well) can bind mouse EFNA4 with a linear ranger of 1.28-32 ng/ml.)
Ephrin A5, Active Protein (Cat# AAA258181)
Full Name
Ephrin A5 Protein, Mouse, Recombinant (His Tag)
Gene Names
Efna5; AL-1; Epl7; RAGS; EFL-5; LERK-7; AV158822; Ephrin-A5
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL.)
SCF, Active Protein (Cat# AAA257884)
Full Name
SCF Protein, Human, Recombinant (aa 1-189, His Tag)
Gene Names
KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
Purity
>92% as determined by SDS-PAGE
Pricing
alpha Amylase enzyme, Active Protein (Cat# AAA75393)
Full Name
alpha Amylase enzyme
Bioactivity
(Folic Acid-BSA is coated at 10ug/ml (100 ul/well) can bind Human FOLR2. The ED50 range )
FOLR2, Active Protein (Cat# AAA48385)
Full Name
Recombinant human FOLR2 protein
Gene Names
FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing
Bioactivity
Membrane Cofactor (CD46), Active Protein (Cat# AAA243727)
Full Name
Recombinant Human Membrane Cofactor Protein (CD46)
Gene Names
CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
Monocyte Chemotactic Protein 4 (MCP4), Active Protein (Cat# AAA146618)
Full Name
Active Monocyte Chemotactic Protein 4 (MCP4)
Gene Names
CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate)
Galectin 2 (GAL2), Active Protein (Cat# AAA153013)
Full Name
Active Galectin 2 (GAL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant BMP2, Human;Antibody: Rabbit Anti-Human BMP2 Ab)
Bone Morphogenetic Protein 2 (BMP2), Active Protein (Cat# AAA149222)
Full Name
Active Bone Morphogenetic Protein 2 (BMP2)
Gene Names
BMP2; BDA2; BMP2A; SSFSC
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
WB (Western Blot)
(Sample: Recombinant IL2Rb, Mouse;Antibody: Rabbit Anti-Mouse IL2Rb Ab)
Interleukin 2 Receptor Beta (IL2Rb), Active Protein (Cat# AAA148239)
Full Name
Active Interleukin 2 Receptor Beta (IL2Rb)
Gene Names
Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Reactivity
Mus musculus (Mouse)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>92%
Pricing
Bioactivity
(Galectin-12 is a member of a family of mammalian lectins known as galectins. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin-12 is predominantly expressed in adipose tissue and detected also in macrophages and other leukocytes. It plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Po GAL12 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL12 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL12, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL12 is 0.195 ug/mL.)
Galectin 12 (GAL12), Active Protein (Cat# AAA152176)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
Integrin Associated Protein (IAP), Active Protein (Cat# AAA148184)
Full Name
Active Integrin Associated Protein (IAP)
Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Reactivity
Mouse
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Complement Component 3 (C3) is a vital component of the complement system that plays a central role in immune response and inflammation. C3 is a beta? globulin synthesized by the liver, whose structure consists of two polypeptide chains, alpha and beta, and is the most abundant complement component in serum. It's been identified that C3 can interact with Complement Factor B (CFB), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C3 and recombinant mouse CFB. Briefly, CFB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CFB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. When Recombinant C3 is immobilized at 2 ug/mL (100 uL/well), the concentration of CFB that produces 50% optimal binding response is found to be approximately 5.96 ug/mL.)
Complement Component 3 (C3), Active Protein (Cat# AAA161793)
Full Name
Active Complement Component 3 (C3)
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Glypican 1 (GPC1) is one of the six glycosylphosphatidylinositol-anchored, cell surface heparan sulfate proteoglycans that acts as a growth factor signaling. Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells. The function of GPC-1 is complex and varied, it can modulate various signaling pathways including Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), fibroblast growth factors (FGF), vascular endothelial growth factor-A (VEGF-A), transforming growth factor-beta (TGF-beta), Wnt, Hedgehog (Hh), and bone morphogenic protein (BMP) through specific interactions with pathway ligands and receptors. Besides, Syndecan 4 (SDC4) has been also identified as an interactor of GPC1), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC1 and recombinant chicken SDC4. Briefly, GPC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SDC4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse GPC1 and recombinant chicken SDC4 was shown in Figure 1, the EC50 for this effect is 0.04 ug/mL.)
Glypican 1 (GPC1), Active Protein (Cat# AAA161825)
Full Name
Active Glypican 1 (GPC1)
Gene Names
Gpc1; AI462976
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
GRO gamma (CXCL3), Active Protein (Cat# AAA214342)
Full Name
Recombinant Rat GRO gamma (CXCL3)
Gene Names
Cxcl3; Cinc2; Cinc-2; Gm1960
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human PDGFRB at 2 ug/ml (100 ul/well) can bind Mouse PDGF-B His, the EC50 of Mouse PDGF-B His is 4-24 ng/mL.)
PDGF-B, Active Protein (Cat# AAA258203)
Full Name
PDGF-B Protein, Mouse, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to bind recombinant human EphrinB2 in a functional ELISA.)
EphB2, Active Protein (Cat# AAA257925)
Full Name
EphB2 Protein, Human, Recombinant (His & hFc Tag)
Gene Names
EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; MGC87492
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153019)
Full Name
Active Galectin 4 (GAL4)
Gene Names
Lgals4; L-36; L36LBI
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)
Galectin 12 (GAL12), Active Protein (Cat# AAA153027)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)
Galectin 12 (GAL12), Active Protein (Cat# AAA153029)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Figure 1. The binding activity of GDNF with GFRa2.)
Glial Cell Line Derived Neurotrophic Factor, Active Protein (Cat# AAA150051)
Full Name
Active Glial Cell Line Derived Neurotrophic Factor (GDNF)
Gene Names
GDNF; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant CTSA, Rat;Antibody: Rabbit Anti-Rat CTSA Ab)
Cathepsin A, Active Protein (Cat# AAA150088)
Full Name
Active Cathepsin A (CTSA)
Reactivity
Rattus norvegicus (Rat)
Applications
Activity Assay, Cell Culture
Purity
>92%
Pricing
Application Data
Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Active Protein (Cat# AAA146599)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
Gene Names
Csf2; Csfgm; GMCSF; Gm-CSf; MGI-IGM
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Application Data
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9), Active Protein (Cat# AAA148133)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9)
Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Neuraminidase (NEU), Active Protein (Cat# AAA148148)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Human EPHB1 is coated at 2 ug/ml (100 ul/well) can bind Human EFNB1 (CAT# ATGP3800) in a Functional ELISA assay.)
EphB1, Active Protein (Cat# AAA48404)
Full Name
Recombinant human EphB1 protein
Gene Names
EPHB1; ELK; NET; Hek6; EPHT2
Applications
Bioactivity, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
CD16a / FCGR3A, Active Protein (Cat# AAA173505)
Full Name
Recombinant Human CD16a / FCGR3A Protein (176 Val, His & AVI tag)
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Purity
> 90 % as determined by reducing SDS-PAGE.
Pricing
Bioactivity
(Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)
Janus Kinase 1 (JAK1), Active Protein (Cat# AAA161890)
Full Name
Active Janus Kinase 1 (JAK1)
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Taste receptor type 1 member 3 (TAS1R3), responsible for mediating taste perception, is a G protein-coupled receptor (GPCR) expressed in gustatory cells located within the taste buds on the tongue. It can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TAS1R3 and recombinant mouse TAS1R1. Briefly, biotin-linked TAS1R3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TAS1R1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TAS1R3 and recombinant mouse TAS1R1 was shown in Figure 1, the EC50 for this effect is 0.31 ug/mL.)
Taste Receptor Type 1 Member 3 (TAS1R3), Active Protein (Cat# AAA161923)
Full Name
Active Taste Receptor Type 1 Member 3 (TAS1R3)
Gene Names
TAS1R3; T1R3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 8-32 ng/mL.)
Oncostatin M/OSM, Active Protein (Cat# AAA258129)
Full Name
Oncostatin M/OSM Protein, Mouse, Recombinant
Gene Names
Osm; OncoM
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
BACE1, Active Protein (Cat# AAA257824)
Full Name
BACE1 Protein, Human, Recombinant
Gene Names
BACE1; ASP2; BACE; HSPC104
Purity
>95% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Sample: Recombinant TNFRSF12A, Human; Antibody: Rabbit Anti-Human TNFRSF12A Ab)
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein (Cat# AAA148180)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Applications
Activity Assay, Cell Culture
Purity
>85%
Pricing
Application Data
(Binding and in vitro activity of murine 18V4F hybridoma antibody.Directly coated chemokines were used here for direct comparisons, however in other experiments CCL3 showed a significantly enhanced signal when biotinylated and coated on streptavidin plates.)
MIP-1 alpha (CCL-3), Active Protein (Cat# AAA76392)
Full Name
Mouse MIP-1 alpha (CCL-3) Recombinant
Gene Names
Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(MERS-CoV Spike RBD is coated at 2 ug/ml (100 ul/well) can bind human DPPIV/CD26 in a functional ELISA assay.)
MERS-CoV Spike, Active Protein (Cat# AAA48981)
Full Name
Recombinant MERS-CoV Spike RBD protein
Applications
Bioactivity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Alpha-2 Antiplasmin, Active Protein (Cat# AAA44856)
Full Name
Alpha-2 Antiplasmin, Human Plasma
Gene Names
SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
Purity
>= 95% (SDS-PAGE)
Pricing
Bioactivity
(Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161783)
Full Name
Active C Reactive Protein (CRP)
Gene Names
Crp; AI255847
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Cystatin A (CSTA) is a member of family 1 of the cystatin superfamily. Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L. For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes. The ratio of cathepsin B and Cystatin A can be used in the differential identification and treatment of patients with prostate carcinoma. The activity of recombinant mouse Cystatin A was measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC in the assay buffer 50 mM Tris, pH 7.0. Papain was diluted to 500 ug/ml in activation buffer 50 mM Tris, 5 mM DTT, pH 7.0 and incubated at room temperature for 15 minutes. The activated papain was diluted to 100 ug/ml in the assay buffer and 20 ul different concentrations of recombinant mouse Cystatin A (MW: 40.9 KD) was incubated with 20 ul 100 ug/ml papain at 37 degree C for 10 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant mouse Cystatin A significantly decreased papain activity. The inhibition IC50 was )
Cystatin A (CSTA), Active Protein (Cat# AAA161736)
Full Name
Active Cystatin A (CSTA)
Gene Names
Csta1; Csta
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
CD40 Ligand (CD40LG), Active Protein (Cat# AAA214215)
Full Name
Recombinant Human CD40 Ligand (CD40LG)
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CCL3, Active Protein (Cat# AAA214246)
Full Name
Recombinant Human CCL3
Gene Names
CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
Purity
>95% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.