Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1750-1800 of 2567 product results
Bioactivity
(Parkinson Disease Protein 7 (PARK7), a small multifunctional protein (20 kDa) containing 189 amino acids, which participates in transcriptional regulation and mitochondrial regulation, and acts as a molecular chaperone, oxidative stress sensor, and glyoxalase. It has also been described as a protein and nucleotide deglycase. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease. Leucine Rich Repeat Kinase 2 (LRRK2) has been identified as an interactor of PARK7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PARK7 and recombinant human LRRK2. Briefly, PARK7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LRRK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PARK7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PARK7 and recombinant human LRRK2 was shown in Figure 1, the EC50 for this effect is 0.16 ug/mL.)
Parkinson Disease Protein 7 (PARK7), Active Protein (Cat# AAA161933)
Full Name
Active Parkinson Disease Protein 7 (PARK7)
Gene Names
PARK7; DJ1; DJ-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant rat TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rrTIMP1 (MW: 22.2 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rrTIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )
Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161745)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
Gene Names
Timp1; Timp; TIMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown in Figure 1, the EC50 for this effect is 2.82 ug/mL.)
Cluster Of Differentiation 28 (CD28), Active Protein (Cat# AAA161757)
Full Name
Active Cluster Of Differentiation 28 (CD28)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant rat GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant rat GSTa3 is >37000 pmol/min/ug.)
Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161768)
Full Name
Active Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta1; Yc1; Gsta3; Gsta5; GST AA; GST 2-2; GST Yc1; GST A3-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 23 or FGF23 is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate and vitamin D metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 decreases the reabsorption and increases excretion of phosphate and suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF23, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF23 and recombinant human FGFR1. Briefly, FGF23 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF23 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FGF23 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.17 ug/mL.)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA161772)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)
Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161662)
Full Name
Active Colony Stimulating Factor 3, Granulocyte (GCSF)
Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF is a monomeric glycoprotein that functions as a cytokine - it is a white blood cell growth factor. It also has some effects on mature cells of the immune system, such as inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GM-CSF and recombinant human CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human GM-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.04 ug/mL.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein (Cat# AAA161668)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
Complement Component 8b (C8b), Active Protein (Cat# AAA161841)
Full Name
Active Complement Component 8b (C8b)
Gene Names
C8B; C82
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of recombinant pig LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1 ug/mL LPS, and treated with certain concentrations (0.625 ug/mL-5 ug/mL) of rpLBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with recombinant pig LBP have shown in Figure 1.)
Lipopolysaccharide Binding Protein (LBP), Active Protein (Cat# AAA161848)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
LBP; LPSBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(S100 calcium-binding protein 9 (S100A9) also known as calgranulin B, is a member of the S100 family of secreted calcium binding proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Lactoferrin (LTF) is one of targets of S100A9, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse S100A9 and recombinant human LTF. Briefly, S100A9 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LTF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse S100A9 and recombinant human LTF was shown in Figure 1, the EC50 for this effect is 0.44 ug/mL.)
S100 Calcium Binding Protein A9 (S100A9), Active Protein (Cat# AAA161859)
Full Name
Active S100 Calcium Binding Protein A9 (S100A9)
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Complement Factor D (CFD) is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity. The full-length (amino acid residues 1-263) of rat CFD was expressed which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 50 mM Tris, 1 M NaCl, pH 7.5 (Assay Buffer), initiated by addition 50 uL of various concentrations of CFD (diluted by Assay Buffer) to 50 ul substrate mixture of 0.2mM Z-Lys-SBzl•HCl and 0.2 mM DTNB. The final well serves as a negative control with no CFD, replaced with 50 ul assay buffer. Then read in kinetic mode for 5 minutes at an absorbance of 405 nm. The specific activity of recombinant rat CFD is > 10000 pmol/min/ug.)
Complement Factor D (CFD), Active Protein (Cat# AAA161865)
Full Name
Active Complement Factor D (CFD)
Gene Names
Cfd; Df; Adn; EVE
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2 dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant mouse PRF1 with 0.9% NaCl, add 50ul a serial dilution of PRF1, 10ul 0.1M CaCl2 to each well, then add 50ul 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 50ul 0.9% NaCl to replace PRF1 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 1. It was obvious that the minimal effective concentration of PRF1 is 5ug/ml.)
Perforin 1 (PRF1), Active Protein (Cat# AAA152181)
Full Name
Active Perforin 1 (PRF1)
Gene Names
Prf1; Pfn; Pfp; Prf-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1beta can induced IL-8 production in A549 cells. To test the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with certain concentrations of IL-1beta for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1beta have shown in Figure1.)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA152227)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Ciliary Neurotrophic Factor (CNTF) is a common extracellular polypeptide hormone who has neuroprotective effects on a variety of central and also peripheral nervous system neurons. It promotes differentiation and maturation of oligodendrocyte precursor ce)
Ciliary Neurotrophic Factor (CNTF), Active Protein (Cat# AAA152949)
Full Name
Active Ciliary Neurotrophic Factor (CNTF)
Gene Names
CNTF; HCNTF
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The chemotactic effect of NAP3 on THP-1 cells)
Chemokine (C-X-C Motif) Ligand 1 (CXCL1), Active Protein (Cat# AAA152959)
Full Name
Active Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(human IL12A is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded b)
Interleukin 12A (IL12A), Active Protein (Cat# AAA152962)
Full Name
Active Interleukin 12A (IL12A)
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.)
Interleukin 2 (IL2), Active Protein (Cat# AAA152964)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.)
Interleukin 2 (IL2), Active Protein (Cat# AAA152965)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; POIL2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells)
Interleukin 2 (IL2), Active Protein (Cat# AAA152966)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; POIL2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(IL-2(Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to r)
Interleukin 2 (IL2), Active Protein (Cat# AAA152967)
Full Name
Active Interleukin 2 (IL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target c)
Leukemia Inhibitory Factor (LIF), Active Protein (Cat# AAA152974)
Full Name
Active Leukemia Inhibitory Factor (LIF)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant human MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rhMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 °C for 2 hours. The activated rhMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 uL of 1 ug/mL rhMMP-1 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human MMP1 is > 140 pmol/min/ug.)
Matrix Metalloproteinase 1 (MMP1), Active Protein (Cat# AAA152977)
Full Name
Active Matrix Metalloproteinase 1 (MMP1)
Gene Names
MMP1; CLG; CLGN
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family .It is a gelatinase A, 72 kDa type IV collagenase which can hydrolyze gelatin under certain conditions.)
Matrix Metalloproteinase 2 (MMP2), Active Protein (Cat# AAA152978)
Full Name
Active Matrix Metalloproteinase 2 (MMP2)
Gene Names
MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upo)
Matrix Metalloproteinase 7 (MMP7), Active Protein (Cat# AAA152979)
Full Name
Active Matrix Metalloproteinase 7 (MMP7)
Gene Names
MMP7; MMP-7; MPSL1; PUMP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152986)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFalpha, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152992)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152993)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNF-beta.)
Tumor Necrosis Factor Beta (TNFb), Active Protein (Cat# AAA152995)
Full Name
Active Tumor Necrosis Factor Beta (TNFb)
Gene Names
LTA; TNFB
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasmi)
Plasminogen Activator, Urokinase Receptor (uPAR), Active Protein (Cat# AAA152998)
Full Name
Active Plasminogen Activator, Urokinase Receptor (uPAR)
Gene Names
Plaur; Cd87; uPAR; u-PAR
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153017)
Full Name
Active Galectin 4 (GAL4)
Gene Names
Lgals4; gal-4
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153018)
Full Name
Active Galectin 4 (GAL4)
Gene Names
LGALS4; L36LBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153019)
Full Name
Active Galectin 4 (GAL4)
Gene Names
Lgals4; L-36; L36LBI
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CR)
Galectin 7 (GAL7), Active Protein (Cat# AAA153021)
Full Name
Active Galectin 7 (GAL7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Galectin 9 (GAL9) is a member of the beta-galactoside-binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused o)
Galectin 9 (GAL9), Active Protein (Cat# AAA153025)
Full Name
Active Galectin 9 (GAL9)
Gene Names
Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)
Galectin 12 (GAL12), Active Protein (Cat# AAA153029)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FS)
Luteinizing Hormone (LH), Active Protein (Cat# AAA153035)
Full Name
Active Luteinizing Hormone (LH)
Gene Names
UCP1; UCP; SLC25A7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153041)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at co)
Caspase 3 (CASP3), Active Protein (Cat# AAA153051)
Full Name
Active Caspase 3 (CASP3)
Gene Names
Casp3; Lice; Yama; CPP32-beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 i)
Toll Like Receptor 4 (TLR4), Active Protein (Cat# AAA153056)
Full Name
Active Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 i)
Toll Like Receptor 4 (TLR4), Active Protein (Cat# AAA153057)
Full Name
Active Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages a)
C ReProtein (CRP), Active Protein (Cat# AAA153061)
Full Name
Active C Reactive Protein (CRP)
Gene Names
Crp; Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not know)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153076)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Gene Names
Vgf; Gm1052
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not know)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153077)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Gene Names
Vgf; Gm1052
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B)
B-Lymphocyte Activation Antigen B7-1 (LAB7-1), Active Protein (Cat# AAA153083)
Full Name
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human)
Neutrophil gelatinase-associated lipocalin (NGAL), Active Protein (Cat# AAA153086)
Full Name
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Gene Names
Lcn2; 24p3; Sip24; AW212229
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)
Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153090)
Full Name
Active Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant GNLY, Human)
Granulysin (GNLY), Active Protein (Cat# AAA153093)
Full Name
Active Granulysin (GNLY)
Gene Names
GNLY; 519; LAG2; NKG5; LAG-2; D2S69E; TLA519
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>80%
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153095)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and he)
Hedgehog Homolog, Sonic (SHH), Active Protein (Cat# AAA153105)
Full Name
Active Hedgehog Homolog, Sonic (SHH)
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide r)
Superoxide Dismutase 1 (SOD1), Active Protein (Cat# AAA153107)
Full Name
Active Superoxide Dismutase 1 (SOD1)
Gene Names
Sod1; Ipo1; SODC; Ipo-1; Sod-1; CuZnSOD; Cu/Zn-SOD; MGC107553; B430204E11Rik; Sod1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.