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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1750-1800 of 2583 product results
Application Data
Interleukin 17 Receptor C (IL17RC), Active Protein (Cat# AAA148123)
Full Name
Active Interleukin 17 Receptor C (IL17RC)
Gene Names
IL17RC; CANDF9; IL17RL; IL17-RL
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC), Active Protein (Cat# AAA148128)
Full Name
Active V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Pricing
Application Data
Neuraminidase (NEU), Active Protein (Cat# AAA148148)
Full Name
Active Neuraminidase (NEU)
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 90%
Pricing
Bioactivity
(Figure 2. Cell proliferation of 3T3 cells after stimulated with AGE.)
Advanced Glycation End Product (AGE), Active Protein (Cat# AAA148250)
Full Name
Active Advanced Glycation End Product (AGE)
Applications
Activity Assay, Cell Culture
Purity
Purity: >90% as determined by SDS-PAGE.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
Pricing
Application Data
(Figure. The chemotactic effect of recombinant human MIP1-on Jurkat cells.)
Macrophage Inflammatory Protein 1 Beta, Active Protein (Cat# AAA150067)
Full Name
Active Macrophage Inflammatory Protein 1 Beta (MIP1b)
Gene Names
CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Figure. The binding activity of TIMP3 with MMP2.Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TIMP3 and recombinant mouse MMP2. Briefly, TIMP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-TIMP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TIMP3 and MMP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Tissue Inhibitors Of Metalloproteinase 3, Active Protein (Cat# AAA150076)
Full Name
Active Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)
Gene Names
Timp3; Timp-3
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Bioactivity
(Figure. The binding activity of IL1b with IL1R1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150094)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; IL-1F2
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
WB (Western Blot)
(Western Blot Sample: Recombinant PIIINP, Human;Antibody: Rabbit Anti-Human PIIINP Ab)
Procollagen III N-Terminal Propeptide, Active Protein (Cat# AAA150095)
Full Name
Active Procollagen III N-Terminal Propeptide (PIIINP)
Gene Names
COL3A1; EDS4A; EDSVASC; PMGEDSV
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Pricing
Bioactivity
(Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) is a member of the TGF beta protein family. Myostatin is a secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MSTN with BMP1.)
Myostatin, Active Protein (Cat# AAA150112)
Full Name
Active Myostatin (MSTN)
Gene Names
MSTN; GDF8; MSLHP
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Fibroblast Growth Factor 10 (FGF10), Active Protein (Cat# AAA150560)
Full Name
Active Fibroblast Growth Factor 10 (FGF10)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
(Semaphorin-3F (SEMA3F) is a member of semaphorins family. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Semaphorins not only guide axons in development, but also have major roles in immune function (classes 4, 6, and 7) and the development of bones. Class 3 semaphorins are one of the most versatile semaphorin classes, in which Sema3a is the most studied. Besides, SEMA3F is deleted or downregulated in many metastatic tumors. To test the effect of SEMA3F on inhibit cell proliferation, human lung carcinoma cells A549 were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of recombinant human SEMA3F. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Proliferation of A549 cells after incubation with SEMA3F for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant SEMA3F for 48h. The result was shown in Figure 2. It was obvious that SEMA3F significantly increased cell viability of A549 cells. (A) A549 cells cultured in DMEM, stimulated with 100ng/mL SEMA3F for 48h; (B) Unstimulated A549 cells cultured in DMEM for 48h.Figure. Cell proliferation of A549 cells inhibtion by stimulating with SEMA3F.)
Semaphorin 3F, Active Protein (Cat# AAA150147)
Full Name
Active Semaphorin 3F (SEMA3F)
Gene Names
SEMA3F; SEMA4; SEMAK; SEMA-IV
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2 dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant mouse PRF1 with 0.9% NaCl, add 50ul a serial dilution of PRF1, 10ul 0.1M CaCl2 to each well, then add 50ul 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 50ul 0.9% NaCl to replace PRF1 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 1. It was obvious that the minimal effective concentration of PRF1 is 5ug/ml.)
Perforin 1 (PRF1), Active Protein (Cat# AAA152181)
Full Name
Active Perforin 1 (PRF1)
Gene Names
Prf1; Pfn; Pfp; Prf-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), Active Protein (Cat# AAA244030)
Full Name
Recombinant Human Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), partial
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
Ephrin-A5 (EFNA5), Active Protein (Cat# AAA243728)
Full Name
Recombinant Human Ephrin-A5 (EFNA5) (Active)
Gene Names
EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Bioactivity
(Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit doe)
Interleukin 3 Receptor Alpha (IL3Ra), Active Protein (Cat# AAA153090)
Full Name
Active Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant GNLY, Human)
Granulysin (GNLY), Active Protein (Cat# AAA153093)
Full Name
Active Granulysin (GNLY)
Gene Names
GNLY; 519; LAG2; NKG5; LAG-2; D2S69E; TLA519
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>80%
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153095)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and he)
Hedgehog Homolog, Sonic (SHH), Active Protein (Cat# AAA153105)
Full Name
Active Hedgehog Homolog, Sonic (SHH)
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide r)
Superoxide Dismutase 1 (SOD1), Active Protein (Cat# AAA153107)
Full Name
Active Superoxide Dismutase 1 (SOD1)
Gene Names
Sod1; Ipo1; SODC; Ipo-1; Sod-1; CuZnSOD; Cu/Zn-SOD; MGC107553; B430204E11Rik; Sod1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin-29 (IL-29) is a member of the helical cytokine family and is a type III interferon. It is also known as IFNlambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 plays an important role in host)
Interleukin 29 (IL29), Active Protein (Cat# AAA153110)
Full Name
Active Interleukin 29 (IL29)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(DHCR7 (7-dehydrocholesterol reductase) is a rate-limiting enzyme in cholesterol synthesis, which is mainly involved in the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol. In the cholesterol biosynthetic pathway, this protease can reduce the C7-)
7-Dehydrocholesterol Reductase (DHCR7), Active Protein (Cat# AAA153112)
Full Name
Active 7-Dehydrocholesterol Reductase (DHCR7)
Gene Names
DHCR7; SLOS
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase IX (CA9) also known as membrane antigen MN and renal cell carcinoma (RCC) associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. rhCA9 correspond)
Carbonic Anhydrase IX (CA9), Active Protein (Cat# AAA153125)
Full Name
Active Carbonic Anhydrase IX (CA9)
Gene Names
CA9; MN; CAIX
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 37, also known as Interleukin 1 Zeta (IL1z), is an anti-inflammatory cytokine. Interleukines are cytokines that make an important part of immune signaling. It belongs to the interleukin-1 family. IL-37 plays a role in protecting the body again)
Interleukin 1 Zeta (IL1z), Active Protein (Cat# AAA153128)
Full Name
Active Interleukin 1 Zeta (IL1z)
Gene Names
IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferati)
Interleukin 1 Family, Member 9 (IL1F9), Active Protein (Cat# AAA153138)
Full Name
Active Interleukin 1 Family, Member 9 (IL1F9)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153017)
Full Name
Active Galectin 4 (GAL4)
Gene Names
Lgals4; gal-4
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153018)
Full Name
Active Galectin 4 (GAL4)
Gene Names
LGALS4; L36LBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153019)
Full Name
Active Galectin 4 (GAL4)
Gene Names
Lgals4; L-36; L36LBI
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CR)
Galectin 7 (GAL7), Active Protein (Cat# AAA153021)
Full Name
Active Galectin 7 (GAL7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Galectin 9 (GAL9) is a member of the beta-galactoside-binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused o)
Galectin 9 (GAL9), Active Protein (Cat# AAA153025)
Full Name
Active Galectin 9 (GAL9)
Gene Names
Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)
Galectin 12 (GAL12), Active Protein (Cat# AAA153029)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FS)
Luteinizing Hormone (LH), Active Protein (Cat# AAA153035)
Full Name
Active Luteinizing Hormone (LH)
Gene Names
UCP1; UCP; SLC25A7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153041)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at co)
Caspase 3 (CASP3), Active Protein (Cat# AAA153051)
Full Name
Active Caspase 3 (CASP3)
Gene Names
Casp3; Lice; Yama; CPP32-beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 i)
Toll Like Receptor 4 (TLR4), Active Protein (Cat# AAA153056)
Full Name
Active Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 i)
Toll Like Receptor 4 (TLR4), Active Protein (Cat# AAA153057)
Full Name
Active Toll Like Receptor 4 (TLR4)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages a)
C ReProtein (CRP), Active Protein (Cat# AAA153061)
Full Name
Active C Reactive Protein (CRP)
Gene Names
Crp; Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not know)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153076)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Gene Names
Vgf; Gm1052
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not know)
VGF Nerve Growth Factor Inducible (VGF), Active Protein (Cat# AAA153077)
Full Name
Active VGF Nerve Growth Factor Inducible (VGF)
Gene Names
Vgf; Gm1052
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B)
B-Lymphocyte Activation Antigen B7-1 (LAB7-1), Active Protein (Cat# AAA153083)
Full Name
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human)
Neutrophil gelatinase-associated lipocalin (NGAL), Active Protein (Cat# AAA153086)
Full Name
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Gene Names
Lcn2; 24p3; Sip24; AW212229
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1beta can induced IL-8 production in A549 cells. To test the bioactivity of IL-1beta, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with certain concentrations of IL-1beta for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1beta have shown in Figure1.)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA152227)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Fibulin 2 (FBLN2) is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined. Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin. It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors. To test the effect of FBLN2 on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant rat FBLN2 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1h at 37 degree C. Proliferation of A549 cells after incubation with FBLN2 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant rat FBLN2 for 72h. The result was shown in Figure 2. It was obvious that FBLN2 significantly inhibit cell viability of A549 cells. The ED50 is 0.97 ug/mL.)
Fibulin 2 (FBLN2), Active Protein (Cat# AAA161910)
Full Name
Active Fibulin 2 (FBLN2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to KAL-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.)
Kallistatin (KAL), Active Protein (Cat# AAA161912)
Full Name
Active Kallistatin (KAL)
Gene Names
SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Ephrin A3 (EFNA3), also known as EFL2, EPLG3, LERK3, Ehk1-L, is a member of the Eph/ephrin tyrosine kinase family. EFNA3 is localized in cell membranes and was highly expressed in brain, skin, esophagus, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, peripheral blood leukocytes. EFNA3, like most genes in the ephrin family, plays a central role in embryonic development can associate with multiple signaling pathways involved in cell growth and tumor cell metastasis. Aberrant regulation of EFNA3 is associated with the occurrence and development of various types of cancer. Ephrin Type A Receptor 4 (EPHA4) is a receptor for members of the ephrin-A family. It can bind to EFNA3. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA3 and recombinant human EPHA4. Briefly, EFNA3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EPHA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNA3 and recombinant human EPHA4 was shown in Figure 1, the EC50 for this effect is 0.4 ug/mL.)
Ephrin A3 (EFNA3), Active Protein (Cat# AAA161916)
Full Name
Active Ephrin A3 (EFNA3)
Gene Names
EFNA3; EFL2; EPLG3; LERK3; Ehk1-L
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Parkinson Disease Protein 7 (PARK7), a small multifunctional protein (20 kDa) containing 189 amino acids, which participates in transcriptional regulation and mitochondrial regulation, and acts as a molecular chaperone, oxidative stress sensor, and glyoxalase. It has also been described as a protein and nucleotide deglycase. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease. Leucine Rich Repeat Kinase 2 (LRRK2) has been identified as an interactor of PARK7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PARK7 and recombinant human LRRK2. Briefly, PARK7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LRRK2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PARK7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PARK7 and recombinant human LRRK2 was shown in Figure 1, the EC50 for this effect is 0.16 ug/mL.)
Parkinson Disease Protein 7 (PARK7), Active Protein (Cat# AAA161933)
Full Name
Active Parkinson Disease Protein 7 (PARK7)
Gene Names
PARK7; DJ1; DJ-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
Complement Component 8b (C8b), Active Protein (Cat# AAA161841)
Full Name
Active Complement Component 8b (C8b)
Gene Names
C8B; C82
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of recombinant pig LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1 ug/mL LPS, and treated with certain concentrations (0.625 ug/mL-5 ug/mL) of rpLBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with recombinant pig LBP have shown in Figure 1.)
Lipopolysaccharide Binding Protein (LBP), Active Protein (Cat# AAA161848)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
LBP; LPSBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(S100 calcium-binding protein 9 (S100A9) also known as calgranulin B, is a member of the S100 family of secreted calcium binding proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Lactoferrin (LTF) is one of targets of S100A9, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse S100A9 and recombinant human LTF. Briefly, S100A9 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LTF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse S100A9 and recombinant human LTF was shown in Figure 1, the EC50 for this effect is 0.44 ug/mL.)
S100 Calcium Binding Protein A9 (S100A9), Active Protein (Cat# AAA161859)
Full Name
Active S100 Calcium Binding Protein A9 (S100A9)
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Complement Factor D (CFD) is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity. The full-length (amino acid residues 1-263) of rat CFD was expressed which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 50 mM Tris, 1 M NaCl, pH 7.5 (Assay Buffer), initiated by addition 50 uL of various concentrations of CFD (diluted by Assay Buffer) to 50 ul substrate mixture of 0.2mM Z-Lys-SBzl•HCl and 0.2 mM DTNB. The final well serves as a negative control with no CFD, replaced with 50 ul assay buffer. Then read in kinetic mode for 5 minutes at an absorbance of 405 nm. The specific activity of recombinant rat CFD is > 10000 pmol/min/ug.)
Complement Factor D (CFD), Active Protein (Cat# AAA161865)
Full Name
Active Complement Factor D (CFD)
Gene Names
Cfd; Df; Adn; EVE
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs. The activity of recombinant human CA13 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA13 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase XIII (CA13), Active Protein (Cat# AAA161906)
Full Name
Active Carbonic Anhydrase XIII (CA13)
Gene Names
CA13; CAXIII
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.