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Active Proteins

AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.


Viewing 300-350 of 2567 product results


product-image-AAA161881_BIOACTIVITY13.jpg Bioactivity (Afamin (AFM, also known as Alpha -Albumin), a bioactive protein, is expressed in the liver and secreted into the bloodstream. Human afamin is a 87 kDa protein which is encoded by the fourth member of the human albumin gene family. Mouse AFM shares 66% aa sequence identity with Full-length human AFM. This protein can bind and transport vitamin E across the blood-brain barrier in body fluids under conditions where the lipoprotein system is not sufficient, it also can act as a carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse AFM and recombinant human WNT3A. Briefly, AFM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to WNT3A-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AFM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse AFM and recombinant human WNT3A was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL.)

Afamin (AFM), Active Protein (Cat# AAA161881)

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Full Name
Active Afamin (AFM)
Gene Names
Afm; Alf; alpha-Alb
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA177978_BIOACTIVITY13.jpg Bioactivity

Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177978)

No reviews yet
Full Name
Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-10His)
Purity
>95% as determined by reducing SDS-PAGE
Pricing

ALKALINE PHOSPHATASE, Active Protein (Cat# AAA50488)

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Full Name
NATIVE CALF ALKALINE PHOSPHATASE
Gene Names
ALPI; IAP
Applications
ELISA
Pricing
product-image-AAA48522_SDS_PAGE15.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Acid phosphatase 1, Active Protein (Cat# AAA48522)

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Full Name
Acid phosphatase 1,1-158aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
ACP1; HAAP; LMWPTP; LMW-PTP
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
product-image-AAA48568_SDS_PAGE15.jpg SDS-PAGE

AKR7A2, Active Protein (Cat# AAA48568)

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Full Name
AKR7A2, 1-359aa, Human, His tag, E. coli (Bioactivity Validated)
Gene Names
AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing

ApoE2, Active Protein (Cat# AAA79218)

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Full Name
Human ApoE2
Gene Names
APOE; AD2; LPG; LDLCQ5
Reactivity
Human
Purity
> 90% by SDS-PAGE and HPLC analyses
Pricing

Adiponectin (Acrp30), Active Protein (Cat# AAA79239)

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Full Name
Mouse Adiponectin (Acrp30)
Gene Names
Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
Purity
> 98% by SDS-PAGE gel and HPLC analyses.
Pricing

ApoE3, Active Protein (Cat# AAA79280)

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Full Name
Human ApoE3
Gene Names
APOE; AD2; LPG; LDLCQ5
Reactivity
Human
Purity
> 90% by SDS-PAGE and HPLC analyses
Pricing

Adiponectin, Active Protein (Cat# AAA78131)

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Full Name
Adiponectin, human, native
Gene Names
ADIPOQ; ADN; APM1; ACRP30
Purity
Affinity and hydrophobic interaction chromatography
Pricing
product-image-AAA268887_AD13.jpg Application Data

COVID 19 Spike S1+S2 Coronavirus, Active Protein (Cat# AAA268887)

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Full Name
SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag)
Purity
>90 % as determined by SDS-PAG
Pricing
product-image-AAA153003_BIOACTIVITY11.jpg Bioactivity (Figure 2. Cell apoptosis of A549 cells after stimulated with IFNb.)

Interferon Beta (IFNb), Active Protein (Cat# AAA153003)

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Full Name
Active Interferon Beta (IFNb)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing

Creatine Kinase MM (CK-MM) >95% Pure, Active Protein (Cat# AAA58215)

No reviews yet
Full Name
Human Creatine Kinase-MM
Gene Names
CKM; CKMM; M-CK
Purity
1 CK band by agarose gel isoenzyme electrophoresis. Molecular weight of the MM fraction is unknown.
Pricing
product-image-AAA258047_AD13.png Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 ug/ml (100 ul/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL)

RNF43, Active Protein (Cat# AAA258047)

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Full Name
RNF43 Protein, Human, Recombinant (hFc Tag)
Gene Names
RNF43; URCC; RNF124
Purity
>90% as determined by SDS-PAGE
Pricing
product-image-AAA150121_AD11.jpg Application Data (Figure. Cell proliferation of Hela cells after stimulated with ANXA2.)

Annexin A2, Active Protein (Cat# AAA150121)

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Full Name
Active Annexin A2 (ANXA2)
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
product-image-AAA161864_BIOACTIVITY13.jpg Bioactivity (Transglutaminase 2 (TGM2), encoded by the TGM2 gene, is belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions. In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP and has intrinsic kinase activity. TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases. The activity of recombinant human TGM2 is measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH in the assay buffer 200 mM MES, 10 mM DTT, 10 mM CaCl2, 100 mM Hydroxylamine Hydrochloride, pH 6.0. The rhTGM2 is diluted to 12.5 ug/ml in assay buffer. Loading into a clear well plate 50 uL of 12.5 ug/mL rhTGM2 and start the reaction by adding 50 uL of 100 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhTGM2. Incubated at 37 degree C for 2 hours and stop the reaction with 400 ul stop solution of 0.37 M FeCl3, 0.67 M HCl, 0.2 M Trichloroacetic Acid. Centrifuge at 2000 rpm for 2 minutes and then load 200 ul of the supernatant into a plate and read at 525 nm (absorbance) in endpoint mode. The specific activity of recombinant human TGM2 is > 800 pmol/min/ug.)

Transglutaminase 2 (TGM2), Active Protein (Cat# AAA161864)

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Full Name
Active Transglutaminase 2 (TGM2)
Gene Names
TGM2; TG2; TGC; GNAH; G-ALPHA-h
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing

Stromal Cell-Derived Factor-1 alpha, Active Protein (Cat# AAA38080)

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Full Name
Recombinant Human Stromal Cell-Derived Factor-1 alpha (CXCL12)
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

GRO/KC, Active Protein (Cat# AAA38092)

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Full Name
Recombinant Mouse GRO/KC (CXCL1)
Gene Names
Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Interleukin-1 beta, Active Protein (Cat# AAA38108)

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Full Name
Recombinant Human Interleukin-1 beta
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Lactate Dehydrogenase 3, Active Protein (Cat# AAA44811)

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Full Name
Lactate Dehydrogenase 3 (LDH-3), Human Erythrocytes
Pricing

Persephin, Active Protein (Cat# AAA39062)

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Full Name
Recombinant Human Persephin
Gene Names
PSPN; PSP
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing

Glutathione S-Transferase Alpha 4, Active Protein (Cat# AAA39148)

No reviews yet
Full Name
Recombinant Human Glutathione S-Transferase Alpha 4, Active
Gene Names
GSTA4; GTA4; GSTA4-4
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing

Cathepsin-B, Active Protein (Cat# AAA39158)

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Full Name
Recombinant Mouse Cathepsin-B, Active
Gene Names
Ctsb; CB
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing

Peroxiredoxin-4, Active Protein (Cat# AAA38812)

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Full Name
Recombinant Human Peroxiredoxin-4
Gene Names
PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing

Interleukin-15, Active Protein (Cat# AAA39137)

No reviews yet
Full Name
Recombinant Human Interleukin-15, HEK
Gene Names
IL15; IL-15
Purity
Greater than 95.0% as determined by SDS-PAGE.

Cathepsin G, Active Protein (Cat# AAA44787)

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Full Name
Cathepsin G, Human Neutrophil
Gene Names
CTSG; CG; CATG
Purity
> 90% (SDS-PAGE)
Pricing
product-image-AAA268895_AD13.jpg Application Data

COVID 19 Spike S1 Coronavirus, Active Protein (Cat# AAA268895)

No reviews yet
Full Name
SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His tag)
Purity
>90% as determined by SDS-PAGE
Pricing

Fibrinogen, Active Protein (Cat# AAA37797)

No reviews yet
Full Name
Mouse Fibrinogen
Gene Names
Fga; Fib; ENSMUSG00000059807
Purity
>95% by SDS-PAGE analysis
Pricing
product-image-AAA75217_SDS_PAGE15.jpg SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)

Alphaenolase, Active Protein (Cat# AAA75217)

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Full Name
Alphaenolase protein
Applications
SDS-PAGE
Purity
> 90% pure
Pricing

Superoxide Dismutase-2, Active Protein (Cat# AAA38823)

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Full Name
Recombinant Human Superoxide Dismutase-2
Gene Names
SOD2; IPOB; MNSOD; MVCD6
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing

Transforming Growth Factor-Beta 1, Active Protein (Cat# AAA38647)

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Full Name
Human Transforming Growth Factor-Beta 1
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing

Beta Defensin -3, Active Protein (Cat# AAA38981)

No reviews yet
Full Name
Recombinant Mouse Beta Defensin -3
Gene Names
Defb3; BD-3
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing

Indian Hedgehog, Active Protein (Cat# AAA39005)

No reviews yet
Full Name
Recombinant Human Indian Hedgehog
Gene Names
IHH; BDA1; HHG2
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Eotaxin, Active Protein (Cat# AAA39096)

No reviews yet
Full Name
Recombinant Rhesus Macaque Eotaxin (CCL11); Eotaxin Rhesus Macaque
Gene Names
CCL11; SCYA11; Eotaxin
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE
Pricing

Glutamic-Oxaloacetic Transaminase 1, Active Protein (Cat# AAA39141)

No reviews yet
Full Name
Recombinant Human Glutamic-Oxaloacetic Transaminase 1, Active
Gene Names
GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing

Carboxylesterase 1D, Active Protein (Cat# AAA39142)

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Full Name
Recombinant Mouse Carboxylesterase 1D
Gene Names
Ces1d; TGH; Ces3
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing

gamma-Glutamyl Transferase, Active Protein (Cat# AAA44875)

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Full Name
gamma-Glutamyl Transferase Bovine Kidney
Pricing

L-Glutamate Dehydrogenase, Active Protein (Cat# AAA44793)

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Full Name
Glutamate Dehydrogenase (GLDH), Bovine Liver
Gene Names
GLUD1; GDH
Pricing

Lactate Dehydrogenase 5, Active Protein (Cat# AAA44813)

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Full Name
Lactate Dehydrogenase 5 (LDH-5), Human Liver
Pricing

Follicle Stimulating Hormone, Active Protein (Cat# AAA44845)

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Full Name
Follicle Stimulating Hormone (FSH), Human Pituitary - High Purity
Purity
≥ 95% as determined by SDS-PAGE
Immunogen affinity purified

Cancer Antigen 242, Active Protein (Cat# AAA44859)

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Full Name
Cancer Antigen 242 (CA-242), Human
Purity
Control Grade
Pricing

Procalcitonin (PCT), Active Protein (Cat# AAA44759)

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Full Name
Procalcitonin (PCT) antigen
Applications
Immunoassay, Control
Purity
>90% (SDS-PAGE)
Pricing
product-image-AAA258009_AD13.png Application Data (Measured by its binding ability in a functional ELISA. Immobilized MICAh at 10 ug/mL (100 uL/well) can bind S4-Fc3L3-NKG2D, the EC50 of S4-Fc3L3-NKG2D is 200-450 ng/mL.)

MICA, Active Protein (Cat# AAA258009)

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Full Name
MICA Protein, Human, Recombinant (His Tag)
Gene Names
MICA; MIC-A; PERB11.1
Purity
>95% as determined by SDS-PAGE
Pricing
product-image-AAA150048_BIOACTIVITY11.png Bioactivity (Figure 1. The binding activity of BMP2 with NOG.)

Bone Morphogenetic Protein 2, Active Protein (Cat# AAA150048)

No reviews yet
Full Name
Active Bone Morphogenetic Protein 2 (BMP2)
Gene Names
BMP2; BDA2; BMP2A; SSFSC
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
product-image-AAA150079_WB8.jpg WB (Western Blot) (Figure 4. Western BlotSample: Active recombinant CEA, HumanAntibody: Rabbit Anti-Human CEA Ab)

Carcinoembryonic Antigen, Active Protein (Cat# AAA150079)

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Full Name
Active Carcinoembryonic Antigen (CEA)
Gene Names
CEACAM5; CEA; CD66e
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing

acidic FGF (FGF-1), Active Protein (Cat# AAA47950)

No reviews yet
Full Name
Recombinant Human Acidic FGF1
Gene Names
fgf1; aFGF; ecgf; fgfa; ecgfa; ecgfb; fgf-1; hbgf1; HBGF-1; glio703; ecgf-beta; fgf-alpha
Purity
>90%
Purification: Purified by heparin affinity chromatography and HPLC; single band at 1 ug on silver-stained SDS-PAGE.
Pricing
product-image-AAA48534_SDS_PAGE15.jpg SDS-PAGE (#ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

AKR1B10, Active Protein (Cat# AAA48534)

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Full Name
AKR1B10, 1-316aa, Human, E Coli (Bioactivity Validated)
Gene Names
AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
Applications
Enzyme Activity, SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
product-image-AAA48390_BIOACTIVITY13.jpg Bioactivity (SARS-CoV S1 Subunit is coated at 5 ug/ml (100 ul/well) can bind Human ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)

COVID 19 Spike S1 Subunit Coronavirus, Active Protein (Cat# AAA48390)

No reviews yet
Full Name
Recombinant SARS-CoV S1 Subunit protein
Applications
Bioactivity, SDS-PAGE
Purity
>90% by SDS-PAGE
Pricing

Artemin, Active Protein (Cat# AAA75568)

No reviews yet
Full Name
Artemin protein
Gene Names
ARTN; EVN; NBN; ENOVIN
Purity
> 98% pure
Pricing

gamma-Glutamyl Transferase, Active Protein (Cat# AAA44798)

No reviews yet
Full Name
Gamma-glutamyl Transferase (gGT), Porcine Kidney
Gene Names
GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732
Purity
Purified

Plasminogen, Active Protein (Cat# AAA44858)

No reviews yet
Full Name
Plasminogen (PLG), Human Plasma
Gene Names
PLG; LPA
Purity
>95% (SDS-PAGE)
Pricing

What Are Active Proteins?


Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).


If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.


Key Applications of Active Proteins


1. Scientific Research


  • Aid in the study of how proteins function in the body

  • Aid in understanding various disease processes


2. Drug Development


  • Powerful tools to investigate how potential drugs interact with specific proteins

  • Ideal for identifying drug targets


3. Cell Culture


  • Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)

  • Can be used to promote cellular development into specific types (differentiation)


4. Diagnostics


  • Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)

  • Note: All products are strictly for research-use only (RUO).


5. Therapeutics


  • Some active proteins are used directly as treatments (e.g., insulin, enzymes)

  • Note: All products are strictly for research-use only (RUO).


6. Vaccine Development


  • Used to create or test vaccines by mimicking parts of viruses or bacteria


7. Biochemical Assays


  • They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests


Why Buy Active Proteins from AAA Biotech?


  • High biological activity – Verified to perform as expected or indicated on datasheet

  • Strict quality control – We are confident in our active proteins’ reliability and consistency

  • High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components

  • Freeze-dried for stability – Long shelf life and straightforward storage

  • Mostly tag-free – Closer to natural/native protein form


FAQ


1. What are active proteins used for in research?


Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.


2. How are AAA Biotech's active proteins validated?


AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.


3. Are these proteins tested for biological activity?


Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.


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