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Active Proteins

AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.


Viewing 200-250 of 2583 product results


gamma-Glutamyl Transferase, Active Protein (Cat# AAA44875)

Full Name
gamma-Glutamyl Transferase Bovine Kidney
Pricing

Procalcitonin (PCT), Active Protein (Cat# AAA44759)

Full Name
Procalcitonin (PCT) antigen
Applications
Immunoassay, Control
Purity
>90% (SDS-PAGE)
Pricing

L-Glutamate Dehydrogenase, Active Protein (Cat# AAA44793)

Full Name
Glutamate Dehydrogenase (GLDH), Bovine Liver
Gene Names
GLUD1; GDH
Pricing

Lactate Dehydrogenase 5, Active Protein (Cat# AAA44813)

Full Name
Lactate Dehydrogenase 5 (LDH-5), Human Liver
Pricing

acidic FGF (FGF-1), Active Protein (Cat# AAA47950)

Full Name
Recombinant Human Acidic FGF1
Gene Names
fgf1; aFGF; ecgf; fgfa; ecgfa; ecgfb; fgf-1; hbgf1; HBGF-1; glio703; ecgf-beta; fgf-alpha
Purity
>90%
Purification: Purified by heparin affinity chromatography and HPLC; single band at 1 ug on silver-stained SDS-PAGE.
Pricing
product-image-AAA75217_SDS_PAGE15.jpg SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)

Alphaenolase, Active Protein (Cat# AAA75217)

Full Name
Alphaenolase protein
Applications
SDS-PAGE
Purity
> 90% pure
Pricing

Beta Defensin -3, Active Protein (Cat# AAA38981)

Full Name
Recombinant Mouse Beta Defensin -3
Gene Names
Defb3; BD-3
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Pricing

Indian Hedgehog, Active Protein (Cat# AAA39005)

Full Name
Recombinant Human Indian Hedgehog
Gene Names
IHH; BDA1; HHG2
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Eotaxin, Active Protein (Cat# AAA39096)

Full Name
Recombinant Rhesus Macaque Eotaxin (CCL11); Eotaxin Rhesus Macaque
Gene Names
CCL11; SCYA11; Eotaxin
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE
Pricing

Glutamic-Oxaloacetic Transaminase 1, Active Protein (Cat# AAA39141)

Full Name
Recombinant Human Glutamic-Oxaloacetic Transaminase 1, Active
Gene Names
GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing

Carboxylesterase 1D, Active Protein (Cat# AAA39142)

Full Name
Recombinant Mouse Carboxylesterase 1D
Gene Names
Ces1d; TGH; Ces3
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing

Fibrinogen, Active Protein (Cat# AAA37797)

Full Name
Mouse Fibrinogen
Gene Names
Fga; Fib; ENSMUSG00000059807
Purity
>95% by SDS-PAGE analysis
Pricing

Superoxide Dismutase-2, Active Protein (Cat# AAA38823)

Full Name
Recombinant Human Superoxide Dismutase-2
Gene Names
SOD2; IPOB; MNSOD; MVCD6
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing

Transforming Growth Factor-Beta 1, Active Protein (Cat# AAA38647)

Full Name
Human Transforming Growth Factor-Beta 1
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing

Artemin, Active Protein (Cat# AAA75568)

Full Name
Artemin protein
Gene Names
ARTN; EVN; NBN; ENOVIN
Purity
> 98% pure
Pricing
product-image-AAA258026_SDS_PAGE15.jpg SDS-PAGE

DNase I, Active Protein (Cat# AAA258026)

Full Name
DNase I Protein, Human, Recombinant (His Tag)
Gene Names
DNASE1; DNL1; DRNI
Purity
>85% as determined by SDS-PAGE
Pricing
product-image-AAA258028_AD13.png Application Data (Measured by its binding ability in a functional ELISA. Immobilized human TMIGD2 at 10 ug/mL (100 uL/well) can bind human HHLA2-Fc, the EC50 of human HHLA2-Fc is 0.3-3ug/mL.)

TMIGD2, Active Protein (Cat# AAA258028)

Full Name
TMIGD2 Protein, Human, Recombinant (His Tag)
Gene Names
TMIGD2; CD28H; IGPR1; IGPR-1
Purity
>85% as determined by Nu-PAGE
Pricing
product-image-AAA214279_AD13.png Application Data

Fractalkine (CX3CL1), Active Protein (Cat# AAA214279)

Full Name
Recombinant Human Fractalkine (CX3CL1)
Gene Names
CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
Purity
>95% as determined by SDS-PAGE
Pricing
product-image-AAA244032_BIOACTIVITY13.png Bioactivity

Spike glycoprotein (S), Active Protein (Cat# AAA244032)

Full Name
Recombinant Human Novel Coronavirus Spike glycoprotein (S), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
product-image-AAA153010_BIOACTIVITY13.jpg Bioactivity (Procollagen II N-Terminal Propeptide is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces, which was are associated wit)

Procollagen II N-Terminal Propeptide (PIINP), Active Protein (Cat# AAA153010)

Full Name
Active Procollagen II N-Terminal Propeptide (PIINP)
Gene Names
COL2A1; AOM; ANFH; SEDC; COL11A3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA161788_BIOACTIVITY13.jpg Bioactivity (PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system through activating its specific membrane-anchored receptor (PRLR). A functional binding ELISA assay was conducted to detect the interaction of recombinant goat PRL and recombinant human PRLR. Briefly, PRL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PRLR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant goat PRL and recombinant human PRLR was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)

Prolactin (PRL), Active Protein (Cat# AAA161788)

Full Name
Active Prolactin (PRL)
Gene Names
PROLACTIN; PRL
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA161811_BIOACTIVITY13.jpg Bioactivity (Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant bovine AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant bovine AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant bovine AP is >3000 U/mg.)

Aprotinin (AP), Active Protein (Cat# AAA161811)

Full Name
Active Aprotinin (AP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA161701_BIOACTIVITY13.jpg Bioactivity (Tissue Inhibitors of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. The activity of recombinant bovine TIMP4 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rbTIMP4 (MW: 24.05 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rbTIMP4 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant bovine TIMP4 significantly decreased rhMMP2 activity. The inhibition IC50 was )

Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), Active Protein (Cat# AAA161701)

Full Name
Active Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing

APRIL, Active Protein (Cat# AAA75589)

Full Name
APRIL protein
Gene Names
TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
Purity
> or = 95% pure by SDS-PAGE gel and HPLC
Pricing
product-image-AAA78521_ACTIVITY15.png Activity (Figure 1 : ACE2 (human)(rec.) (AAA78521) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (AAA78521 ) is coated on an ELISA plate at 1 ug/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) is added (starting at a concentration of 500 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).)

ACE2, Active Protein (Cat# AAA78521)

Full Name
Human ACE2 Protein (DYKDDDDK Tag)
Applications
Functional Assay
Purity
>95% (SDS-PAGE)
Pricing

Apo Transferrin (ATF), Active Protein (Cat# AAA76530)

Full Name
Human Apo Transferrin (ATF)
Reactivity
Human
Purity
Greater than 98.0% as determined by Cellulose Acetate Electrophoresis.
Pricing

Neuron-specific Enolase, Active Protein (Cat# AAA78132)

Full Name
Neuron-specific enolase (NSE)
Gene Names
Eno2; NSE; RNEN3
Applications
ELISA
Purity
Ion-exchange chromatography and gel-filtration.
Pricing
product-image-AAA177979_BIOACTIVITY13.jpg Bioactivity

Angiotensin-Converting Enzyme 2/ACE-2, Active Protein (Cat# AAA177979)

Full Name
Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-Fc)
Purity
>95% as determined by reducing SDS-PAGE
Pricing

Prostate Specific Antigen, Active Protein (Cat# AAA59627)

Full Name
Prostate Specific Antigen, Human
Gene Names
KLK3; APS; PSA; hK3; KLK2A1
Purity
>/= 95% by SDS-PAGE
Pricing

Peroxiredoxin-2, Active Protein (Cat# AAA38698)

Full Name
Recombinant Human Peroxiredoxin-2
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing

Cyclophilin-A, Active Protein (Cat# AAA38559)

Full Name
Recombinant Human Cyclophilin-A
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing

Macrophage Inflammatory protein-3 alpha, Active Protein (Cat# AAA38581)

Full Name
Recombinant Mouse Macrophage Inflammatory protein-3 alpha (CCL20)
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Platelet Derived Growth Factor-AA, Active Protein (Cat# AAA38597)

Full Name
Recombinant Human Platelet Derived Growth Factor-AA, Yeast
Gene Names
PDGFA; PDGF1; PDGF-A
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing

Kinase A regulatory subunit-1 alpha, Active Protein (Cat# AAA38384)

Full Name
Recombinant Human Protein Kinase A regulatory subunit-1 alpha
Gene Names
PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing

Peroxisome Proliferator Activated Receptor Gamma, Active Protein (Cat# AAA38395)

Full Name
Recombinant Human Peroxisome Proliferator Activated Receptor Gamma
Gene Names
PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing

TIE-2 Fc Chimera, Active Protein (Cat# AAA38404)

Full Name
Recombinant Human TIE-2 Fc Chimera
Gene Names
TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
Purity
Greater than 90.0% as determined by(a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
Pricing

Guanylate Kinase 1, Active Protein (Cat# AAA38408)

Full Name
Recombinant Human Guanylate Kinase 1, Active
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing

Placenta Growth Factor-2, Active Protein (Cat# AAA38199)

Full Name
Recombinant Human Placenta Growth Factor-2
Gene Names
PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
Purity
Greater than 80.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Isocitrate Dehydrogenase-1, Active Protein (Cat# AAA38258)

Full Name
Recombinant Yeast Isocitrate Dehydrogenase-1
Gene Names
ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Leptin, Active Protein (Cat# AAA38126)

Full Name
Recombinant Ovine Leptin
Gene Names
LEP; ob
Purity
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Vascular Endothelial Growth Factor, Active Protein (Cat# AAA38128)

Full Name
Recombinant Human Vascular Endothelial Growth Factor
Gene Names
VEGFA; VPF; VEGF; MVCD1
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

soluble CD40 Ligand/TRAP, Active Protein (Cat# AAA38130)

Full Name
Recombinant Human soluble CD40 Ligand/TRAP
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Interleukin-17A, Active Protein (Cat# AAA38132)

Full Name
Recombinant Human Interleukin-17A
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing

Neurotrophin-3, Active Protein (Cat# AAA38136)

Full Name
Recombinant Human Neurotrophin-3
Gene Names
NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Interleukin-4, Active Protein (Cat# AAA38143)

Full Name
Recombinant Mouse Interleukin-4
Gene Names
Il4; Il-4; BSF-1
Purity
Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Flt3-Ligand, Active Protein (Cat# AAA38161)

Full Name
Recombinant Human Flt3-Ligand
Gene Names
FLT3LG; FL; FLT3L
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Interleukin-9, Active Protein (Cat# AAA38171)

Full Name
Recombinant Human Interleukin-9
Gene Names
IL9; P40; HP40; IL-9
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Vascular Endothelial Growth Factor, Active Protein (Cat# AAA38186)

Full Name
Recombinant Rat Vascular Endothelial Growth Factor
Gene Names
Vegfa; VPF; Vegf; VEGF-A; VEGF164
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing

BGrowth Hormone, Active Protein (Cat# AAA38653)

Full Name
Recombinant Bovine Growth Hormone
Gene Names
GH1; GH
Purity
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Pricing

Carboxypeptidase-B, Active Protein (Cat# AAA38666)

Full Name
Recombinant Rat Carboxypeptidase-B
Gene Names
Cpb1; Cpb
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing

What Are Active Proteins?


Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).


If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.


Key Applications of Active Proteins


1. Scientific Research


  • Aid in the study of how proteins function in the body

  • Aid in understanding various disease processes


2. Drug Development


  • Powerful tools to investigate how potential drugs interact with specific proteins

  • Ideal for identifying drug targets


3. Cell Culture


  • Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)

  • Can be used to promote cellular development into specific types (differentiation)


4. Diagnostics


  • Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)

  • Note: All products are strictly for research-use only (RUO).


5. Therapeutics


  • Some active proteins are used directly as treatments (e.g., insulin, enzymes)

  • Note: All products are strictly for research-use only (RUO).


6. Vaccine Development


  • Used to create or test vaccines by mimicking parts of viruses or bacteria


7. Biochemical Assays


  • They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests


Why Buy Active Proteins from AAA Biotech?


  • High biological activity – Verified to perform as expected or indicated on datasheet

  • Strict quality control – We are confident in our active proteins’ reliability and consistency

  • High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components

  • Freeze-dried for stability – Long shelf life and straightforward storage

  • Mostly tag-free – Closer to natural/native protein form


FAQ


1. What are active proteins used for in research?


Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.


2. How are AAA Biotech's active proteins validated?


AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.


3. Are these proteins tested for biological activity?


Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.


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