Search results for "Recombinant Proteins"

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Bioactivity (Serum Amyloid A2 (SAA2) is a multifunctional apolipoprotein that belongs to SAA family. SAA2 is produced by hepatocytes in response to pro-inflammatory cytokines and is the most prominent members of the acute phase response (APR) during which their serum levels rise dramatically after trauma, infection and other stimuli. Besides, Apolipoprotein C3 (APOC3) has been identified as an interactor of SAA2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SAA2 and recombinant human APOC3. Briefly, SAA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to APOC3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SAA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SAA2 and recombinant human APOC3 was shown in Figure 1, and this effect was in a dose dependent manner.)

Serum Amyloid A2 (SAA2), Active Protein (Cat# AAA161860)

Full Name

Active Serum Amyloid A2 (SAA2)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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ELISA (Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1ug/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.)

SARS-CoV-2 (COVID-19) Spike S2, Monoclonal Recombinant Antibody (Cat# AAA40985)

Full Name

SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1B8]

Reactivity

Virus

Applications

ELISA

Purity

SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

Pricing

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WB (Western Blot) (Figure 4. Western BlotSample: Recombinant TUBb, Human;Antibody: Rabbit Anti-Human TUBb Ab)

Tubulin Beta (TUBb), Active Protein (Cat# AAA148197)

Full Name

Active Tubulin Beta (TUBb)

Gene Names

TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56

Reactivity

Homo sapiens (Human)

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

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Bioactivity (Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. GSTM1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant chicken GSTM1, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chicken GSTM1 is >2500 pmol/min/ug.)

Glutathione S Transferase Mu 1 (GSTM1), Active Protein (Cat# AAA161759)

Full Name

Active Glutathione S Transferase Mu 1 (GSTM1)

Applications

Activity Assay, Cell Culture

Purity

Greater than 97% by SDS-PAGE

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Raji cells using anti-Integrin alpha V/ITGAV antibody (AAA125610).Overlay histogram showing Raji cells stained with AAA125610 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha V/ITGAV Antibody (AAA125610,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Integrin alpha V/ITGAV, Polyclonal Antibody (Cat# AAA125610)

Full Name

Anti-Integrin alpha V/ITGAV Antibody

Gene Names

ITGAV; CD51; MSK8; VNRA; VTNR

Reactivity

Human

Applications

Direct ELISA, Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation. The activity of recombinant human CA1 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA1 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

Carbonic Anhydrase I (CA1), Active Protein (Cat# AAA161796)

Full Name

Active Carbonic Anhydrase I (CA1)

Gene Names

CA1; CAB; CA-I; Car1

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Rat Liver Tissue; Primary Ab: 10ug/ml Mouse AntiRat IFNg AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Interferon Gamma (IFNg), Monoclonal Antibody (Cat# AAA151439)

Full Name

Monoclonal Antibody to Interferon Gamma (IFNg)

Gene Names

Ifng; IFNG2

Reactivity

Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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CD73, Monoclonal Antibody (Cat# AAA23926)

Full Name

CD73 (Immuno-Oncology Target)

Gene Names

NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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Bioactivity (Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)

Bioactivity (G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. The activity of G-CSF is usually measured by a cell proliferation assay using M-NFS60 mouse myelogenous leukemia lymphoblast cells. M-NFS60 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant pig G-CSF. After incubated for 4 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 degree C. Proliferation of M-NFS-60 cells after incubation with G-CSF for 4 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant pig G-CSF for 4 days. The result was shown in Figure 2. It was obvious that G-CSF significantly increased cell viability of M-NFS-60 cells. The EC50 is 1 ug/ml)

Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161664)

Full Name

Active Colony Stimulating Factor 3, Granulocyte (GCSF)

Gene Names

CSF3; G-CSF

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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PKM2, Monoclonal Antibody (Cat# AAA320349)

Full Name

PKM2 Mouse mAb

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

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WB (Western Blot) (Western Blot; Sample: Lane1: Mouse Skeletal muscle lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Skeletal muscle lysate; Lane4: Canine Heart lysate; Lane5: Gallus Skeletal muscle lysate; Lane6: Bovine Heart lysate; Lane7: Rabbit Skeletal muscle lysatePrimary Ab: 0.5ug/ml Mouse AntiHuman ACTC1 AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Actin Alpha 1, Cardiac Muscle (ACTC1), Monoclonal Antibody (Cat# AAA21194)

Full Name

Monoclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1)

Gene Names

ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4

Reactivity

Human, Mouse, Rat, Rabbit, Dog, Pig, Bovine, Chicken

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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WB (Western Blot) (Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Porcine Heart lysate Primary Ab: 2ug/ml Mouse AntiHuman Hsp27 Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

Heat Shock Protein 27 (Hsp27), Monoclonal Antibody (Cat# AAA21186)

Full Name

Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Pig

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of U87 cells using anti-NDUFA9 antibody (AAA19886).Overlay histogram showing U87 cells stained with AAA19886 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA9 Antibody (AAA19886, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 13. IHC analysis of NDUFA9 using anti-NDUFA9 antibody (AAA19886).NDUFA9 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA9 Antibody (AAA19886) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: rat RH35 whole cell lysates,Lane 4: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA9 antigen affinity purified polyclonal antibody (#AAA19886) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDUFA9 at approximately 36 kDa. The expected band size for NDUFA9 is at 43 kDa.)

NDUFA9, Polyclonal Antibody (Cat# AAA19886)

Full Name

Anti-NDUFA9 Antibody Picoband

Gene Names

NDUFA9; CC6; CI39k; CI-39k; NDUFS2L; SDR22E1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

ADAM 10, Recombinant Protein (Cat# AAA58771)

Full Name

ADAM 10

Purity

>95% by SDS-PAGE

Pricing

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COVID 19 Spike S1 Coronavirus, Active Protein (Cat# AAA78534)

Full Name

Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)

Applications

Functional Assay

Purity

>90% by SDS-PAGE.

Pricing

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DDX3X, Polyclonal Antibody (Cat# AAA37648)

Full Name

DDX3X Polyclonal Antibody

Gene Names

DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purification

Pricing

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COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA48384)

Full Name

Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein

Applications

Bioactivity, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

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ELISA (Figure 3 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, 35-580. An ELISA was performed using human SARS-CoV nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.)

COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA40899)

Full Name

SARS-CoV-2 (COVID-19) Nucleoprotein

Reactivity

Virus

Applications

Western Blot, Immunofluorescence

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of RAW264.7 cells using CD68 antibody (AAA28140) at dilution of 1:250. Blue: DAPI for nuclear staining.)

CD68, Polyclonal Antibody (Cat# AAA28140)

Full Name

CD68

Gene Names

CD68; GP110; LAMP4; SCARD1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Insulin Like Growth Factor 1 (IGF1), Recombinant Protein (Cat# AAA152133)

Full Name

Eukaryotic Insulin Like Growth Factor 1 (IGF1)

Gene Names

IGF1; IGFI; IGF-I; IGF1A

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

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B-Cell Activating Factor (BAFF), Recombinant Protein (Cat# AAA152211)

Full Name

Recombinant B-Cell Activating Factor (BAFF)

Gene Names

TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

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Bioactivity (Microsomal human cytochrome P450 17A1 (CYP17A1, 17alpha-hydroxylase, 17,20-lyase) belongs to the cytochrome P450 super family, is expressed in the adrenals and gonads, with minor amounts in the brain, placenta, and heart. CYP17A1 enzyme operates at a key juncture of human steroidogenesis, controlling the levels of mineralocorticoids influencing blood pressure, glucocorticoids involved in immune and stress responses, and androgens and estrogens involved in development and homeostasis of reproductive tissues. Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) has been identified as an interactor of CYP17A1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CYP17A1 and recombinant human ErbB2. Briefly, CYP17A1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ErbB2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP17A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CYP17A1 and recombinant human ErbB2 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)

Cytochrome P450 17A1 (CYP17A1), Active Protein (Cat# AAA161874)

Full Name

Active Cytochrome P450 17A1 (CYP17A1)

Gene Names

CYP17A1; CPT7; CYP17; S17AH; P450C17

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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IP (Immunoprecipitation) (MME was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate4 uL anti-MME mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MME mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 85 kDaObserved band size :100 kDa)

CD10/Neprilysin, Monoclonal Antibody (Cat# AAA258847)

Full Name

Anti-CD10/Neprilysin Antibody, Mouse Monoclonal

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Protein A

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

CD137/4-1BB/TNFRSF9, Monoclonal Antibody (Cat# AAA23920)

Full Name

CD137/4-1BB/TNFRSF9

Gene Names

TNFRSF9; ILA; 4-1BB; CD137; CDw137

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IHC (Immunohistochemisry) (DAB staining on IHCP;Sample: Human Spinal cord Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

High Mobility Group Protein 1 (HMGB1), Monoclonal Antibody (Cat# AAA151530)

Full Name

Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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FCM (Flow Cytometry) (Overlay histogram showing A549 cells stained with AAA28644 (green line).The cells were fixedwith 2% paraformaldehyde (10 min).The cells were then icubated in 2% bovine serumalbumin to block non-specific protein-protein interactions followed by the antibody (AAA28644, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC.Isotype control antibody (blue line) was rabbit IgG1(1ug/1x10^6 cells) used under the same conditions.Acquisition of >10, 000 events was performed.)

ROR1, Polyclonal Antibody (Cat# AAA28644)

Full Name

ROR1 Antibody

Gene Names

ROR1; NTRKR1; dJ537F10.1

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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Bioactivity (Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 uL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 degree C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/ug.)

Hyaluronidase (HAase), Active Protein (Cat# AAA161835)

Full Name

Active Hyaluronidase (HAase)

Gene Names

HYAL1; NAT6; LUCA1; HYAL-1

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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Bioactivity (GLB1 is a lysosomal beta -galactosidase that hydrolyzes the terminal beta -galactose from ganglioside and keratan sulfate. Defects in this gene are the causes of lysosomal storage diseases for GM1-gangliosidosis and Morquio B syndrome (also known as mucopolysaccharidosis IVB). In GM1 gangliosidosis, GM1 ganglioside accumulates in the neurons of the central nervous system, because of the deficiency of lysosomal beta -galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display severe skeletal dysostosis multiplex because of an accumulation of keratan sulfate. The activity assay of GLB1 was measured by its ability to cleave a peptide substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The reaction was performed in 50 mM Sodium Citrate, pH 3.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 1.2 mM Substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse GLB1 is >17000 pmol/min/ug.)

Galactosidase Beta (GLb), Active Protein (Cat# AAA161714)

Full Name

Active Galactosidase Beta (GLb)

Gene Names

Glb1; Bge; Bgl; Bgs; Bgt; Bgl-e; Bgl-s; Bgl-t; AW125515; C130097A14Rik

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHCP;Sample: Rat Liver Tissue; Primary Ab: 30ug/ml Mouse AntiRat IL1a AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Interleukin 1 Alpha (IL1a), Monoclonal Antibody (Cat# AAA21178)

Full Name

Monoclonal Antibody to Interleukin 1 Alpha (IL1a)

Gene Names

Il1a; IL-1 alpha

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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Bioactivity (Figure 2. Cell proliferation of M-NFS-60 cells after stimulated with G-CSF.)

Bioactivity (G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. The activity of G-CSF is usually measured by a cell proliferation assay using M-NFS60 mouse myelogenous leukemia lymphoblast cells. M-NFS60 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant mouse G-CSF. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 degree C. Proliferation of M-NFS-60 cells after incubation with G-CSF for 3 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant mouse G-CSF for 3 days. The result was shown in Figure 2. It was obvious that G-CSF significantly increased cell viability of M-NFS-60 cells. The EC50 is 15.8-41 ng/ml.)

Colony Stimulating Factor 3, Granulocyte (GCSF), Active Protein (Cat# AAA161663)

Full Name

Active Colony Stimulating Factor 3, Granulocyte (GCSF)

Gene Names

Csf3; Csfg; G-CSF; MGI-IG

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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WB (Western Blot) (Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Plasma; Lane3: Rat Pancreas lysate; Lane4: Rat Liver lysate Primary Ab: 5ug/ml Mouse AntiRat HRG Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

Histidine Rich Glycoprotein (HRG), Monoclonal Antibody (Cat# AAA21207)

Full Name

Monoclonal Antibody to Histidine Rich Glycoprotein (HRG)

Gene Names

LOC681544; Hrg; RNHRG2

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHCP;Sample: Human Lung cancer Tissue; Primary Ab: 30ug/ml Mouse AntiHuman BNP AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Brain Natriuretic Peptide (BNP), Monoclonal Antibody (Cat# AAA21182)

Full Name

Monoclonal Antibody to Brain Natriuretic Peptide (BNP)

Gene Names

NPPB; BNP

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHCP;Sample: Mouse Stomach Tissue; Primary Ab: 40ug/ml Mouse AntiMouse RETN AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Resistin (RETN), Monoclonal Antibody (Cat# AAA21189)

Full Name

Monoclonal Antibody to Resistin (RETN)

Gene Names

Retn; ADSF; Rstn; Xcp4; Fizz3

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHCP;Sample: Human Ovarian cancer Tissue; Primary Ab: 20ug/ml Mouse AntiHuman CD147 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Cluster Of Differentiation 147 (CD147), Monoclonal Antibody (Cat# AAA21195)

Full Name

Monoclonal Antibody to Cluster Of Differentiation 147 (CD147)

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Protein A + Protein G affinity chromatography

Pricing

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Bioactivity (carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5(Assay Buffer), initiated by addition 50 uL of various concentrations of CES1 (dilute by Assay Buffer) to 50 uL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400 nm.)

Carboxylesterase 1 (CES1), Active Protein (Cat# AAA152179)

Full Name

Active Carboxylesterase 1 (CES1)

Gene Names

CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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SPHK1, Polyclonal Antibody (Cat# AAA19422)

Full Name

Anti-SPHK1 Antibody Picoband

Gene Names

SPHK1; SPHK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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ELISA (Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (AAA40982) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40982 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.)

SARS-CoV-2 (COVID-19) S1 RBD, Monoclonal Recombinant Antibody (Cat# AAA40982)

Full Name

SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T4P5-H12]

Reactivity

Virus

Applications

ELISA

Purity

SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

Pricing

SAA, ELISA Kit (Cat# AAA105651)

Full Name

Mouse SAA ELISA Kit

Gene Names

Saa1; Saa2; Saa-1

Reactivity

Mouse

Applications

Sandwich ELISA

Pricing

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mCherry, Recombinant Protein (Cat# AAA120553)

Full Name

Recombinant mCherry Protein, N-His

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

EGFR, CLIA Kit (Cat# AAA318590)

Full Name

EGFR (Human) LumiAb ELISA Kit

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human

Pricing

RAGE, ELISA Kit (Cat# AAA30737)

Full Name

RAGE (Human) OmniKine ELISA Kit

Gene Names

AGER; RAGE; SCARJ1

Reactivity

Human

Pricing

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Bioactivity (ADP-Glo assay for SMARCA4 (658-1328) activity (Data from ICE Bioscience Inc.) 100 uM ATP and 10 nM DNA was incubated with different concentrations of SMARCA4 (658-1328) protein in a 10 ul reaction system containing 20 mM HEPES pH 7.5,10 mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, 10 ul ADP-Glo Reagent was added to the products and incubated for 1 hour. Then 20 ul Kinase Detection Reagent incubated for 1 hour. All the operations and reactions were performed at RT. Luminescence measurement is collected by BMG.)

SMARCA4, Active Protein (Cat# AAA60347)

Full Name

Recombinant SMARCA4 (658-1328) protein

Pricing

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Bioactivity (HTRF Assay for Recombinant YES1 activity. 1 uM TK substrate was incubated with different concentrations of YES1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)