Cynthia's Blogs
A Comprehensive Guide to ELISA Sensitivity and Specificity
Key Takeaways
Sensitivity in ELISA is critical for demonstrating the extent of true positives.
Specificity is used to measure the extent to...
Mitigating Cross-Reactivity In Antibody Testing: Precise Strategies For Reliable Results
Key Takeaways
Cross-reactivity in antibody testing is caused by unintended binding to chemically or structurally similar antigens, undermining assay and diagnostic...
Colorimetric vs Fluorometric ELISA: Which Detection Method is Better?
The detection method is a major choice when it comes to designing an ELISA. Two
commonly used methods are colorimetric and...
Bispecific Antibodies: What They Are and Their Applications
Key Takeaways
Bispecific antibodies enable a single molecule to bind two different targets
at once (compared to a traditional antibody that can...
Common ELISA Mistakes and How to Avoid Them
Enzyme-Linked Immunosorbent Assays (ELISAs) are one of the most useful tools in
life science research due to their sensitivity and specificity....
How to Design an ELISA Experiment: Step-by-Step Guide
Key Takeaways
ELISA experiment design demands precision throughout, from antibody pair
selection to sample prep and signal detection.
Sandwich ELISA offers the highest...
Sandwich ELISA Explained: Principle, Protocols, and Best Practices
Key TakeawaysSandwich ELISA is a highly specific and sensitive protein quantification method utilizing two antibodies that bind to different epitopes...
All Types of Antibody Isotypes Explained: IgG, IgA, IgM, IgE, and IgD
Key Takeaways
IgG is the most abundant, long-lasting antibody isotype. Also, it is able
to cross the placenta for facilitating fetal...
Direct ELISA Vs Indirect ELISA: When to Use Each Method
Key Takeaways
Direct ELISA uses one labeled primary antibody; Indirect ELISA uses a
primary + reporter-labeled secondary antibody.
Direct ELISA: faster, simpler, lower...
Antinuclear Antibodies (ANA): Meaning, Test, and What Results Show
Summary Box
ANA (Antinuclear Antibodies) are autoantibodies targeting cell nuclei,
central to autoimmune disease diagnostics.
Multiple subtypes (e.g., anti-dsDNA, anti-Ro, anti-Scl-70) correlate with
different...
What Are Antigens? What are Its Types, Functions and Importance
Understanding Antigens: Types, Functions, & Importance
Key TakeawaysAntigens are substances that trigger your immune system to respond and defend your body. They...
ELISA vs. Western Blot: Which Is Superior In The “Protein Science” Battle?
When you need to detect or quantify a specific protein—such as LC3, troponin, or alkaline phosphatase as some examples—two powerhouse...
What Are The Differences Between “Monoclonal” Antibodies And “Recombinant” Antibodies?
Today, antibodies are the unsung heroes that are ubiquitous in modern research. These are used in everything - from cancer...
Role of Activated Protein Kinases in Biomedical Research
Protein kinases play a critical role in cell communication. They serve as
molecular “switches”, primarily by donating phosphate groups to...