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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 1550-1600 of 2583 product results
Neurturin, Active Protein (Cat# AAA79278)
Full Name
Human Neurturin
Gene Names
NRTN; NTN
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analysis
Pricing
Application Data
VEGF188, Active Protein (Cat# AAA79300)
Full Name
Mouse VEGF188
Gene Names
Vegfa; Vpf; Vegf
Reactivity
Mouse
Purity
> 95% by SDS-PAGE & silver stain
Pricing
Application Data
(SDS-PAGE analysis of recombinant rat soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
Podoplanin, soluble, Active Protein (Cat# AAA79303)
Full Name
Rat Podoplanin, soluble
Gene Names
Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha
Reactivity
Rat
Purity
> 98% by SDS-PAGE & silver stain
Pricing
Application Data
CXCL2, Active Protein (Cat# AAA214224)
Full Name
Recombinant Human CXCL2
Gene Names
CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Adiponectin (Acrp30), Active Protein (Cat# AAA214306)
Full Name
Recombinant Human Adiponectin (Acrp30)
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL33, Active Protein (Cat# AAA214317)
Full Name
Recombinant Mouse IL33
Gene Names
Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL6, Active Protein (Cat# AAA214318)
Full Name
Recombinant Mouse IL6
Gene Names
Il6; Il-6
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
Beta-Defensin 3 (DEFB3), Active Protein (Cat# AAA214325)
Full Name
Recombinant Mouse Beta-Defensin 3 (DEFB3)
Gene Names
Defb3; BD-3
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
EGF, Active Protein (Cat# AAA214337)
Full Name
Recombinant Rat EGF
Purity
>95% as determined by SDS-PAGE
Pricing
BUB1B, Active Protein (Cat# AAA214346)
Full Name
Recombinant Human BUB1B (N-GST tag)
Gene Names
BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
Reactivity
Human (H. sapiens)
Pricing
Application Data
IL19, Active Protein (Cat# AAA214302)
Full Name
Recombinant Human IL19
Gene Names
IL19; MDA1; NG.1; ZMDA1; IL-10C
Purity
>95% as determined by SDS-PAGE
Pricing
Bioactivity
(FGF22(Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cel)
Fibroblast Growth Factor 22 (FGF22), Active Protein (Cat# AAA153118)
Full Name
Active Fibroblast Growth Factor 22 (FGF22)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Plasminogen Activator Inhibitor 1 (PAI1) is a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI 1 is the principal inhibitor of urokinase type plasminogen activator (uPA) and tissue type PA. As important regulators of extracell)
Plasminogen Activator Inhibitor 1 (PAI1), Active Protein (Cat# AAA153037)
Full Name
Active Plasminogen Activator Inhibitor 1 (PAI1)
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Vitronectin (VTN ) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide)
Vitronectin (VTN), Active Protein (Cat# AAA153067)
Full Name
Active Vitronectin (VTN)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5)
Glutathione S Transferase Pi (GSTp), Active Protein (Cat# AAA153069)
Full Name
Active Glutathione S Transferase Pi (GSTp)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153094)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Ink phagocytosis test.)
Interleukin 34 (IL34), Active Protein (Cat# AAA153108)
Full Name
Active Interleukin 34 (IL34)
Gene Names
Il34; AI593503; 2010004A03Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Monocyte Chemotactic Protein 1 (MCP1), also known as C-C motif chemokine 2(CCL2), is a member of the beta (C-C) subfamily of chemokines that is a chemoattractant for monocytes and basophils. Mouse CCL2 is secreted as a 14 kDa glycoprotein monomer but nonc)
Monocyte Chemotactic Protein 1 (MCP1), Active Protein (Cat# AAA152975)
Full Name
Active Monocyte Chemotactic Protein 1 (MCP1)
Gene Names
Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. It is used for numer)
Trypsin (TRY), Active Protein (Cat# AAA153009)
Full Name
Active Trypsin (TRY)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL8 in V- bottom shaped 96-well microtiter plate.)
Galectin 8 (GAL8), Active Protein (Cat# AAA153023)
Full Name
Active Galectin 8 (GAL8)
Gene Names
Lgals8; Lgals-8; AI326142; D13Ertd524e; 1200015E08Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(SP-D (surfactant protein-D) is a 43 kDa member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that further places it in a subset of an expanded group of proteins termed defense collagens. SP-D is known to bind both SIRP alpha and the calreticulin/CD91 complex on macrophages. When the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRP alpha, generating a signal that downmodulates the inflammatory response. When virtually all CRDs are occupied by ligand, however, free CRDs are not available for SIRP alpha binding. The activity of the recombinant human SPD was measured by its ability to bind fluorescein-conjugated E. coli bioparticles. The rhSPD was diluted to 10 ug/ml in assay buffer of 20 mM Tris, 137 mM NaCl, 1 mM CaC12, pH7.4 and fluorescein-conjugated E. coli was diluted to 7*108 cells/ml. Equal volume of 10 ug/ml rhSPD and fluorescein-conjugated E. coli were mixed and incubated at room temperature for 1h. 10 ul mixture was took onto the slide and observed under the fluorescence microscopy. The result was shown in figure 1, the rhSPD could bind fluorescein-conjugated E. coli bioparticles.)
Surfactant Associated Protein D (SPD), Active Protein (Cat# AAA161827)
Full Name
Active Surfactant Associated Protein D (SPD)
Gene Names
SFTPD; SP-D; PSP-D; SFTP4; COLEC7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissuenonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant mouse ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 (assay buffer), ainitiated by addition 50 ul of 0.3 ug/ml ALPL (diluted by assay buffer) to 50 uL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse ALPL is >11000 pmol/min/ug.)
Alkaline Phosphatase, Tissue-nonspecific (ALPL), Active Protein (Cat# AAA161830)
Full Name
Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
Gene Names
Alpl; ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), is a divergent member of the Transforming Growth Factor beta (TGF-beta) superfamily. Human GDF-15 shares 66% and 68% amino acid sequence identity with the rat and mouse proteins, respectively. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF15 has been shown to have various functions, including inhibition of Tumor Necrosis Factor alpha (TNF-alpha) production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. A functional binding ELISA assay was conducted to detect the interaction of recombinant rat GDF-15 and recombinant human GP9. Briefly, GDF-15 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GP9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GDF-15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GDF-15 and GP9 was shown in Figure 1, the EC50 for this effect is 0.47 ug/mL.)
Growth Differentiation Factor 15 (GDF15), Active Protein (Cat# AAA161875)
Full Name
Active Growth Differentiation Factor 15 (GDF15)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Amine Oxidase, Copper Containing 3 (AOC3) is a copper amine oxidase with a topaquinone cofactor. AOC3 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. AOC3 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. The enzyme is sensitive to inhibition by semicarbazide. AOC3 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver. The activity of recombinant human AOC3 was measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of AOC3 (diluted by assay buffer) to 50 ul substrate mixture of 2 mM Benzylamine, 2 units/mL HRP and 100 uM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.)
Amine Oxidase, Copper Containing 3 (AOC3), Active Protein (Cat# AAA161882)
Full Name
Active Amine Oxidase, Copper Containing 3 (AOC3)
Gene Names
AOC3; HPAO; SSAO; VAP1; VAP-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Apolipoprotein M (APOM) is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of lipocalin family proteins. It functions as a component of lipoprotein particles which play essential roles in fatty acid and cholesterol transport and metabolism. Alternative splicing generates a short isoform that lacks the N-terminal 72 amino acids. APOM is produced primarily by hepatocytes but also by renal tubule epithelial cells. The signal peptide is not cleaved and is required for APOM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney. The activity of recombinant mouse APOM was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in APOM. APOM was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 400, 200, 100, 30, 10, 3, 1 and 0.5 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rmAPOM and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rmAPOM and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >300 uM.)
Apolipoprotein M (APOM), Active Protein (Cat# AAA161884)
Full Name
Active Apolipoprotein M (APOM)
Gene Names
Apom; G3a; NG20; 1190010O19Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA4 is a GPIanchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor. The activity of recombinant rat CA4 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA4 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase IV (CA4), Active Protein (Cat# AAA161902)
Full Name
Active Carbonic Anhydrase IV (CA4)
Gene Names
Car4; Ca4
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Sample: Active recombinant SOD4, Mouse)
Superoxide Dismutase Copper Chaperone, Active Protein (Cat# AAA161750)
Full Name
Active Superoxide Dismutase Copper Chaperone
Gene Names
Ccs; Ccsd
Reactivity
Mus musculus (Mouse)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant rat CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161777)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
Car2; Ca2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Parathyroid hormone (PTH) is an 84-amino-acid peptide hormone that is secreted by the parathyroid gland. PTH plays an osteogenic role by acting directly on mesenchymal stem cells, cells with an osteoblastic lineage, osteocytes, and T cells. It also participates as an osteoclast by indirectly acting on osteoclast precursor cells and osteoclasts and directly acting on T cells. PTH is major endocrine regulators of vitamin D metabolism. It proves, with data, this hormone a potent down-regulator of VDR. A functional binding ELISA assay was conducted to detect the interaction of recombinant human PTH and recombinant mouse VDR. Briefly, PTH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to VDR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PTH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PTH and recombinant mouse VDR was shown in Figure 1, the EC50 for this effect is 0.26 ug/mL.)
Parathyroid Hormone (PTH), Active Protein (Cat# AAA161795)
Full Name
Active Parathyroid Hormone (PTH)
Gene Names
PTH; PTH1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant pig AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 ul 4 mg/mL trypsin diluted by 1mM HCl to 160 ul assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 ul of 1mg/ml BAPNA. Include a substrate blank containing 160 ul assay buffer, 20 ul 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 uL of 1mg/ml substrate. Rapidly mixing at 25 degree C, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant pig AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25 degree C for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant pig AP is >600 U/mg.)
Aprotinin (AP), Active Protein (Cat# AAA161813)
Full Name
Active Aprotinin (AP)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Urokinase Plasminogen Activator (uPA), also known as u-plasminogen activator or urokinase, is a highly-specific serine protease from the peptidase S1 family that cleaves plasminogen to form plasmin making it a key player in the plasminogen activator (PA) system. Expression of uPA is minimal in normal cells but is increased several fold in tumor cells by extracellular stimuli elevated in cancer and corresponds to poor outcomes in several types of cancer. Therefore, uPA has been identified as an excellent target for therapeutic development through inhibition of protease activity or though inhibition of uPA-dependent signaling while in complex with uPA receptor (uPAR). The activity assay of uPA was measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The reaction was performed in 50 mM Tris, 0.01% Tween-20, pH 8.5 (Assay Buffer), ainitiated by addition 50 uL of 1.5 ug/ml uPA (diluted by Assay Buffer) to 50 uL of 200 uM Substrate. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse uPA is >2000 pmol/min/ug.)
Plasminogen Activator, Urokinase (uPA), Active Protein (Cat# AAA161706)
Full Name
Active Plasminogen Activator, Urokinase (uPA)
Gene Names
Plau; uPA; u-PA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant human Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 2), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant human Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.)
Annexin V (ANXA5), Active Protein (Cat# AAA161721)
Full Name
Active Annexin V (ANXA5)
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 degree C for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-beta-D-glucosaminidase. The enzyme activity of N-acetyl-beta-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-beta-D-glucosaminidase release.)
Complement Component 5a (C5a), Active Protein (Cat# AAA161727)
Full Name
Active Complement Component 5a (C5a)
Gene Names
Hc; C5; He; C5a
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
Calpain 1 (CAPN1), Active Protein (Cat# AAA161457)
Full Name
Active Calpain 1 (CAPN1)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant chicken BMP4)
Bone Morphogenetic Protein 4 (BMP4), Active Protein (Cat# AAA161653)
Full Name
Active Bone Morphogenetic Protein 4 (BMP4)
Gene Names
BMP4; BMP-4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
CD40, Active Protein (Cat# AAA75431)
Full Name
CD40 protein (Mouse)
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Purity
> 98% pure
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 ug/ml (100 ul/well) can bind human EFNB1 Fc chimera with a linear ranger of 1.56-25 ng/ml.)
Ephrin-B1, Active Protein (Cat# AAA257947)
Full Name
Ephrin-B1 Protein, Human, Recombinant (His & hFc Tag)
Gene Names
EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1--1 ug/mL in the presence of 2.5 ug/mL of rmKlotho beta.)
FGF21, Active Protein (Cat# AAA257950)
Full Name
FGF21 Protein, Human, Recombinant (His Tag)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.04-0.4 ug/mL in the presence of 14 ng/mL human IGFII.)
IGFBP4, Active Protein (Cat# AAA257955)
Full Name
IGFBP4 Protein, Human, Recombinant (His Tag)
Gene Names
IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 ug/mL.)
Transferrin, Active Protein (Cat# AAA257958)
Full Name
Transferrin Protein, Human, Recombinant (His Tag)
Gene Names
TF; TFQTL1; PRO1557; PRO2086
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD7-His at 10 ug/ml (100 ul /well) can bind biotinylated SECTM1-His, The EC50 of biotinylated SECTM1-His is 11-26ng/ml.)
CD7, Active Protein (Cat# AAA257959)
Full Name
CD7 Protein, Human, Recombinant (His Tag)
Gene Names
CD7; GP40; TP41; Tp40; LEU-9
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-0.8 ug/ml in the presence of 10 ng/mL of Recombinant human NGF.)
TrkA, Active Protein (Cat# AAA257961)
Full Name
TrkA Protein, Human, Recombinant (aa 285-413, His Tag)
Gene Names
NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200 at 1 ug/ml (100 ul/well) can bind human CD200R1 / Fc Chimera with a linear range of 0.12-16 ng/ml.)
CD200R, Active Protein (Cat# AAA257969)
Full Name
CD200R Protein, Human, Recombinant (His & hFc Tag)
Gene Names
CD200R1; OX2R; MOX2R; CD200R; HCRTR2
Purity
>94% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human EPHA3-His at 10 ug/ml (100 ul/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6.2-14.6 ng/ml.)
EphA3, Active Protein (Cat# AAA257975)
Full Name
EphA3 Protein, Human, Recombinant (His Tag)
Gene Names
EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 1-6 ng/mL.)
FGF6, Active Protein (Cat# AAA257976)
Full Name
FGF6 Protein, Human, Recombinant
Gene Names
FGF6; HST2; HBGF-6
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human human B7-H2 at 1 ug/ml (100 ul/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.)
ICOS ligand, Active Protein (Cat# AAA257977)
Full Name
ICOS ligand Protein, Human, Recombinant (ECD, His Tag)
Gene Names
ICOSLG; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Purity
>98% as determined by SDS-PAGE
Pricing
Application Data
(1. Immobilized Human ERBB3 His at 2 ug/ml (100 ul/well) can bind Human NRG1 Fc, the EC50 of Human NRG1 Fc is 8-40 ng/mL. 2. Immobilized Rhesus ERBB3 His at 2 ug/ml (100 ul/well) can bind Human NRG1 Fc, the EC50 of Human NRG1 Fc is 20-80 ng/mL.)
NRG1 Beta 1, Active Protein (Cat# AAA257981)
Full Name
NRG1 Beta 1 Protein, Human, Recombinant (ECD, Fc Tag)
Gene Names
NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131
Purity
>85% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.)
SIRP alpha, Active Protein (Cat# AAA257985)
Full Name
SIRP alpha Protein, Human, Recombinant (G75A, ECD, His Tag)
Gene Names
SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
Purity
>98% as determined by SDS-PAGE
Pricing
SDS-PAGE
RAGE, Active Protein (Cat# AAA257986)
Full Name
RAGE Protein, Human, Recombinant (His Tag)
Gene Names
AGER; RAGE; SCARJ1
Purity
>98% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.